Qian Guo

Zhejiang University of Technology

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Qi Shen

University of Science and Technology of China

W. Wu

University of Nottingham

Ruijuan Wang

Nanjing Tech University

Ya‐Ping Xue

Zhejiang University of Technology

S. De Jong

Unilever (Netherlands)

Yu‐Guo Zheng

Zhejiang University of Technology

Hailong Yu

Shanghai Academy of Agricultural Sciences

C. Boucon

Unilever Foods Innovation Centre

Zhiyong Feng

Xi'an Jiaotong University

Feng Cheng

Bozhou People's Hospital

Cooperative Institutions

Shanghai Academy of Agricultural Sciences

Zhejiang University of Technology

Chinese Academy of Sciences

Vrije Universiteit Brussel

Ministry of Agriculture and Rural Affairs

Zhejiang University

Shanghai Jiao Tong University

University of Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Zhejiang Gongshang University

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The serine/threonine protein kinase VSKM1 promotes cold stress resistance of the postharvest Volvariella volvacea by enhancing mitoribosome activity

Postharvest Biology and Technology (2023)

Ming Gong Tingting Zheng Ying Wang Ying‐Ying Wu Qian Guo

Volvariella volvacea

10.1016/j.postharvbio.2023.112465

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Citations (3)

Advances in the Study of Agaricus blazei

Shiyongjun xuebao (2004)

Qian Guo Changyan Zhou Chunyan Song

Advances in the study of Agaricus blazei including the biological characteristics, the cultivation technology, the nutritent value and medicinal effeciency, as well as the pollution of cadmiun were revviewed in this paper.

Agaricus

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Citations (1)

Evaluation of air cleanliness and microbial loads in designated clean areas within a Pleurotus eryngii commercial cultivation facility.

Shiyongjun xuebao (2010)

Lin Wei-wei Wu Xiaojin Qian Guo Mingwen Zhao Ruijuan Wang

The numbers of dust particles of different diameter and the microbial loads in seven designated clean areas within a Pleurotus eryngii commercial cultivation facility were determined and compared to the ISO 14644 standard of air cleanliness.We also compared the level of contamination occurring among the mushroom cultivation bottles and the microbial loads within the facility during the production period from March to September,2009.Our data indicated a relatively high level of cleanliness in the different clean areas during the test period.Significant positive correlations were found between the number of dust particles,mould counts,the sampling elevation and ambient temperature.A significant positive correlation was observed between bacterial and mould counts and the number of dust particles.Microbial counts increased with increasing outside air temperature,and the level of contamination among the mushroom cultivation bottles peaked during June.Clean areas should be maintained using cooling methods,dust removal and disinfection in order to control microbial counts especially during seasons when outside air temperatures are high.Our data provide a useful reference when the levels of cleanliness in designated clean areas in mushroom growing facilities are set at achieving contamination rates 8‰.

Pleurotus eryngii

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Citations (7)

Effects of Temperature on Pleurotus eryngii Fruit Body Development During Artificial Cultivation in Bottles

Shiyongjun xuebao (2009)

Hailong Yu Qian Guo Yang Juan Zhiyong Feng

The effects of temperature on three stages of Pleurotus eryngii fruit body development(mycelial recovery following removal of the substrate surface layer,primordium formation and fruit body maturation) were determined during artificial cultivation in bottles.The rates of mycelial recovery and primordium formation were faster with increasing temperature but the number of primordia produced was unaffected.Fruit body development was more rapid at 17 ℃,but highest yields were recorded at 16 ℃.

Pleurotus eryngii

Primordium

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[Classification of Children with Attention-Deficit/Hyperactivity Disorder and Typically Developing Children Based on Electroencephalogram Principal Component Analysis and k-Nearest Neighbor].

PubMed (2016)

Jiaojiao Yang Qian Guo Wenjie Li Suhong Wang Ling Zou

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Streamlining Design, Engineering, and Applications of Nicotinamide Riboside Kinase for Sustainable Biosynthesis of Nicotinamide Mononucleotide in Flow

ACS Sustainable Chemistry & Engineering (2023)

Feng Cheng Xiaohu Wu Huan Li Kexin Li Qian Guo

The market demand for β-nicotinamide mononucleotide (NMN), an antiaging health product, is rapidly expanding. Consequently, there is an urgent requirement for an environment-friendly and economical approach to synthesize NMN. In this study, we develop a biocatalytic synthesis method for the production of valuable NMN from chemically synthesized nicotinamide riboside (NR). To enhance the efficiency of the process, the biocatalyst human nicotinamide riboside kinase (HS-NRK) was engineered by introducing a beneficial substitution (G13S) near the ATP-binding site. As a result, a 100% conversion of 50 g L–1 NR was achieved by using the engineered variant NRKG13S. However, the NMN yield was limited to 78% (39 g L–1) due to biodegradation by endogenous enzymes present in Escherichia coli. To overcome this challenge, we identified and knocked out the deoD gene for NMN biodegradation. Through protein engineering and metabolic engineering, we achieved a high NMN yield of 93% within 2 h using an effective whole-cell biocatalysis approach. Finally, an immobilized biocatalyst in continuous operation was developed, which facilitated the enzymatic process and enabled the cost-effective and environmentally attractive production of NMN.

Nicotinamide mononucleotide

Metabolic Engineering

Biocatalysis

Commodity chemicals

Riboside

Niacinamide

10.1021/acssuschemeng.3c02833

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Citations (6)

Exploratory chemometric analysis of the classification of pharmaceutical substances based on chromatographic data

Journal of Chromatography A (2000)

Ann Detroyer Vera Schoonjans Frederik Questier Yvan Vander Heyden András Borosy

Chemometrics

Projection pursuit

10.1016/s0021-9673(00)00803-7

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Citations (64)

Improvement of an enzymatic cascade synthesis of nicotinamide mononucleotide via protein engineering and reaction‐process reinforcement

Biotechnology Journal (2024)

Feng Peng Jian Hong Jie Cui Ya‐Ni An Qian Guo

Enzymatic synthesis of β-nicotinamide mononucleotide (NMN) from D-ribose has garnered widespread attention due to its cheap material, the use of mild reaction conditions, and the ability to produce highly pure products with the desired optical properties. However, the overall NMN yield of this method is impeded by the low activity of rate-limiting enzymes. The ribose-phosphate diphosphokinase (PRS) and nicotinamide phosphoribosyltransferase (NAMPT), that control the rate of the reaction, were engineered to improve the reaction efficacy. The actives of mutants PRS-H150Q and NAMPT-Y15S were 334% and 57% higher than that of their corresponding wild-type enzymes, respectively. Furthermore, by adding pyrophosphatase, the byproduct pyrophosphate which can inhibit the activity of NAMPT was degraded, leading to a 6.72% increase in NMN yield. Following with reaction-process reinforcement, a high yield of 8.10 g L

Nicotinamide mononucleotide

Protein Engineering

10.1002/biot.202300748

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Citations (8)

Structure preserving feature selection in PARAFAC using a genetic algorithm and Procrustes analysis

Chemometrics and Intelligent Laboratory Systems (2002)

W. Wu Qian Guo D.L. Massart C. Boucon S. De Jong

Procrustes analysis

Data set

Feature (linguistics)

10.1016/s0169-7439(02)00105-3