Heated glucose is mutagenic to Salmonella typhimurium TA 100 in the absence of S-9 mix. For identifying unknown mutagens in heated glucose (dry solid, 200 degrees C, 20 min), reaction with isopropylideneguanosine (IPG) was followed by isolation and characterization of the mutagen-IPG adduct. Two adducts, glyoxal-IPG and 8-hydroxy-IPG, were identified in the reaction mixture by this technique. To elucidate the mechanism of this hydroxylation reaction, we investigated the abilities of various agents to hydroxylate deoxyguanosine or guanine base in DNA. Various reducing agents, metals, asbestoses, polyphenols, aminophenols, and X-ray were effective for hydroxylation, and an oxygen radical seems to be the reactive species. For sensitive detection of 8-hydroxyguanine, a monoclonal antibody for it was prepared.

Hydroxylation

Deoxyguanosine

Glyoxal

10.1289/ehp.8667111

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Citations (150)

Nucleotide Sequence of the Regulatory Region of malB Operons in E. coli

The Journal of Biochemistry (1983)

Mariko Ohsumi Takao Sekiya Susumu Nishimura Misao Ohki

The nucleotide sequence of a cloned section of the Escherichia coli chromosome containing the promoter regions of the malB divergent operons was determined. The region of the proximal gene, malE of the malEFG operon, was identified on the basis of the known amino acid sequence of the precursor molecule of maltose-binding protein. The region of malK, the proximal gene of the malKlamB operon, was deduced from the observation that a cloned segment contains an amino-terminal portion of the malK gene. The non-coding region between malE and malK is 299 base pairs long and contains two long GC clusters. Another feature of this region that may be related to the regulation of gene expression is the presence of two palindromic structures between the GC clusters. The DNA regions binding to cyclic AMP binding protein were determined by a method using polyacrylamide gel electrophoresis. The sites are thought to be located close to GC clusters.

Coding region

Gene cluster

10.1093/oxfordjournals.jbchem.a134335

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Citations (6)

CCDC 194811: Experimental Crystal Structure Determination

The Cambridge Structural Database (2003)

M. Tanaka Susumu Nishimura Makoto Oba Yusuke Demizu Masaki Kurihara

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Crystal (programming language)

10.5517/cc6jq7n

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Citations (0)

Purification and characterization of AUA specific isoleucine transfer ribonucleic acid from Escherichia coli B

Biochemistry (1974)

Fumio Harada Susumu Nishimura

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTPurification and characterization of AUA specific isoleucine transfer ribonucleic acid from Escherichia coli BFumio Harada and Susumu NishimuraCite this: Biochemistry 1974, 13, 2, 300–307Publication Date (Print):January 15, 1974Publication History Published online1 May 2002Published inissue 15 January 1974https://pubs.acs.org/doi/10.1021/bi00699a011https://doi.org/10.1021/bi00699a011research-articleACS PublicationsRequest reuse permissionsArticle Views135Altmetric-Citations73LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose Get e-Alerts

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Isoleucine

Proxy (statistics)

10.1021/bi00699a011

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Citations (85)

Selective modification of 4-thiouridylate residue in Escherichia coli transfer RNA with cyanogen bromide

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis (1970)

Mineo Saneyoshi Susumu Nishimura

Cyanogen bromide

Cytidine

Inosine

Cyanogen

10.1016/0005-2787(70)90158-9

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Citations (51)