Ching‐Yi Tsai

Kaohsiung Medical University

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Tse‐Hua Tan

National Health Research Institutes

Huai‐Chia Chuang

National Health Research Institutes

Kuo‐Pin Chuang

National Pingtung University of Science and Technology

Edward J. Usherwood

Dartmouth College

Lemmuel L. Tayo

Mapúa University

Chia‐Hsin Hsueh

National Health Research Institutes

Weijun Zhang

Army Medical University

Harvey M. Santos

University of Queensland

Vijay Bhoj

University of Pennsylvania

Yi‐Ming Chen

Taichung Veterans General Hospital

Cooperative Institutions

Federation of American Societies for Experimental Biology

Kaohsiung Medical University

University of Pennsylvania

Kaohsiung Medical University Chung-Ho Memorial Hospital

National Health Research Institutes

National Taiwan University

National Yang Ming Chiao Tung University

National Pingtung University of Science and Technology

National Institutes of Health

China Medical University

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FASEB BioAdvances (2023)

Yousef Abu‐Amer Igor Bado Kim E. Barrett Chris Curran Glyn Dawson

10.1096/fba.1327

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Strain-Dependent Requirement for IFN-γ for Respiratory Control and Immunotherapy in Murine Gammaherpesvirus Infection

Viral Immunology (2011)

Ching‐Yi Tsai Zhuting Hu Weijun Zhang Edward J. Usherwood

Interferon-γ (IFN-γ) and perforin (pfp) are important effector mechanisms used by CD8 T cells to clear virus-infected cells. In this study, we used IFN-γ/pfp double knockout mice to address if these two effector molecules play redundant roles in the control of acute infection with murine gammaherpesvirus-68 (MHV-68) in BALB/C mice. Perforin knockout (KO) mice and wild-type mice cleared infectious virus from the lungs, even following high-dose infection. However, the IFN-γ KO and IFN-γ/pfp double knockout (DKO) groups had higher virus titers in the lungs at day 10 post-infection, and both groups had higher mortality rates. In IFN-γ/pfp DKO mice, the virus titer and mortality rate were significant higher than in IFN-γ KO mice, indicating a role for perforin in protection from disease. WT mice given IFN-γ blocking antibody also showed significantly higher viral titers. In contrast, IFN-γ KO mice on a C57BL/6 background controlled respiratory infection comparably to wild-type mice. These data show that perforin plays a redundant role in the control of virus replication, but IFN-γ plays an essential role in BALB/C mice infected with MHV-68. We conclude that there is a marked strain-dependent difference in the effector mechanisms needed to control acute MHV-68 infection between C57BL/6 and BALB/C mice. In addition we show that immune therapy that re-establishes viral control after spontaneous reactivation in CD4-deficient mice depends upon perforin in C57BL/6 mice but IFN-γ in BALB/C mice.

Knockout mouse

10.1089/vim.2011.0004

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Citations (19)

Postoperative Stroke in Patients After Spinal Anesthesia and Disparate Response Patterns of Carotid or Cerebral Blood Flow and Baroreflex Functionality to Spinal Bupivacaine in Rats

Research Square (Research Square) (2021)

Yan‐Yuen Poon Yueh‐Wei Liu Ya‐Hui Huang Samuel H.H. Chan Ching‐Yi Tsai

Abstract Spinal anesthesia is generally accepted as an effective and safe practice. Three rare incidents of postoperative cerebral infarction after surgery under spinal anesthesia with bupivacaine prompted us to assess whether spinal bupivacaine may impact carotid or cerebral blood flow and baroreflex functionality. We found that all three patients shared common pathology of stenosis or atheromatous lesions in the carotid or middle cerebral artery. In a companion animal study, we further observed that subarachnoid application of bupivacaine that reached low thoracic spinal cord in male Sprague‐Dawley rats elicited an initial (Phase I) reduction in mean arterial pressure (MAP), carotid blood flow (CBF) and baroreflexmediated sympathetic vasomotor tone, all of which returned to baseline in Phase II. Whereas heart rate (HR) exhibited sustained reduction, cardiac vagal baroreflex, baroreflex efficiency index (BEI) and tissue perfusion and oxygen in cerebral cortex supplied by middle cerebral artery remained unaltered during both phases. However, in one‐third of animals studied, Phase II gave way to a Phase III characterized by secondary hypotension and depressed baroreflex‐mediated sympathetic vasomotor tone, along with continuous decline in HR, sustained cardiac vagal baroreflex, decreased BEI, and reduction in CBF and tissue perfusion or oxygen in cerebral cortex. We conclude that carotid and cerebral blood flow can be compromised after spinal anesthesia, and impaired baroreflex‐mediated sympathetic vasomotor tone that leads to hypotension plays a contributory role.

Vasomotor

10.21203/rs.3.rs-142324/v1

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Activation of spinal nociceptin receptors induces cardiovascular depression and antinociception in an independent manner in mice

Proceedings for Annual Meeting of The Japanese Pharmacological Society (2018)

Ya‐Hui Huang Yan‐Yuen Poon Ching‐Yi Tsai

Nociceptin receptor was discovered in 1994 and was designated opioid-like receptor (ORL-1) because it shares a high homology of amino acid sequence with opioid receptors although its affinities for opioid ligands are greatly reduced. The overall effect of ORL-1 activation leads to reduced neuronal excitability. Anatomical studies showed that ORL-1 is localized in brain or spinal nuclei that are related to cardiovascular or nociceptive responses, although the effects of nociceptin on those two responses are equivocal. Taking advantage that intrathecal application of nociceptin could simultaneously activate ORL-1 present in dorsal horn neurons and sympathetic preganglionic neurons in the intermediolateral (IML) column, the present study was designed to decipher whether the cardiovascular and antinociceptive responses elicited by nociceptin in the spinal cord are inter-related. We also investigated the participation of the baroreflex in nociceptin-induced cardiovascular responses.

Nociceptin receptor

10.1254/jpssuppl.wcp2018.0_po3-3-7

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LpxD gene knockout elicits protection to Litopenaeus vannamei, white shrimp, against Vibrio parahaemolyticus infection

Aquaculture International (2019)

Ching‐Yi Tsai Harvey M. Santos Shao‐Yang Hu Cheng Yu Sang Ciara Alyssa S. Yanuaria

Litopenaeus

Penaeus

Gene knockout

White spot syndrome

10.1007/s10499-019-00398-y

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Citations (10)

Retinoic acid modulation of CD8 effector and memory differentiation. (110.4)

The Journal of Immunology (2012)

S. Rameeza Allie Weijun Zhang Ching‐Yi Tsai Yu‐Chi Lee Randolph J. Noelle

Abstract A plethora of work implicates important effects of the Vitamin A derivative, retinoic acid (RA), in myeloid differentiation, while fewer studies explore the role of RA on lymphoid cells. Most work has looked at the role of RA on CD4 T cells, focusing on regulatory CD4 T cell differentiation and maintenance of this phenotype along with some Th1 functions. There is little information about the role of RA in CD8 T cell differentiation, and even less on cell-intrinsic effects in the CD8 T cell. In this study we explored the role of RA on effector and memory differentiation in a direct and cell intrinsic manner in the context of vaccinia virus infection. We used dominant negative RA alpha-receptor (RARα DN) expressing mice and bone marrow chimeric mice containing both WT and RARα DN compartments. We observed the loss of the short-lived effector cell phenotype and an enhanced memory precursor phenotype at the effector and contraction phase of the CD8 response in the absence of RA signaling. Further, the lack of RA increased the proportion of central memory CD8s. Vitamin A is used in conjunction with various vaccines (e.g. Measles vaccine), these results will elucidate the effect of RA in shaping the effector and memory response helping to tailor the supplement to better enhance the outcome of vaccination.

10.4049/jimmunol.188.supp.110.4

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Chemotherapy Treatment Patterns, Health Care Costs, and Mortality of Lung Cancer Patients in Taiwan – A Longitudinal Study Between 2000 and 2008

Value in Health (2013)

Fu‐Wen Liang S.Y Chen Ching‐Yi Tsai Min‐Hua Jen Chung‐Yi Li

Vinorelbine

Interquartile range

10.1016/j.jval.2013.08.468

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Immunogenicity and Protective Activity of Pigeon Circovirus Recombinant Capsid Protein Virus-Like Particles (PiCV rCap-VLPs) in Pigeons (Columba livia) Experimentally Infected with PiCV

Vaccines (2021)

Huai-Ying Huang Benji Brayan Ilagan Silva Shen-Pang Tsai Ching‐Yi Tsai Yu‐Chang Tyan

Pigeon circovirus (PiCV) is the most recurrent virus diagnosed in pigeons and is among the major causative agents of young pigeon disease syndrome (YPDS). Due to the lack of an established laboratory protocol for PiCV cultivation, development of prophylaxis is hampered. Alternatively, virus-like particles (VLPs), which closely resemble native viruses but lack the viral genetic material, can be generated using a wide range of expression systems and are shown to have strong immunogenicity. Therefore, the use of VLPs provides a promising prospect for vaccine development. In this study, transfected human embryonic kidney (HEK-293) cells, a mammalian expression system, were used to express the PiCV capsid protein (Cap), which is a major component of PiCV and believed to contain antibody epitopes, to obtain self-assembled VLPs. The VLPs were observed to have a spherical morphology with diameters ranging from 12 to 26 nm. Subcutaneous immunization of pigeons with 100 µg PiCV rCap-VLPs supplemented with water-in-oil-in-water (W/O/W) adjuvant induced specific antibodies against PiCV. Observations of the cytokine expression and T-cell proliferation levels in spleen samples showed significantly higher T-cell proliferation and IFN- γ expression in pigeons immunized with VLPs compared to the controls (p < 0.05). Experimentally infected pigeons that were vaccinated with VLPs also showed no detectable viral titer. The results of the current study demonstrated the potential use of PiCV rCap-VLPs as an effective vaccine candidate against PiCV.

Circovirus

10.3390/vaccines9020098

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Citations (8)

Influence of pigeon interferon alpha (PiIFN-α) on pigeon circovirus (PiCV) replication and cytokine expression in Columba livia

Veterinary Microbiology (2020)

Harvey M. Santos Chia Chi Chen Ching‐Yi Tsai Yao Ching Hsish Feng Chiao Chung

Bursa of Fabricius

Circovirus

10.1016/j.vetmic.2020.108591

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Citations (16)