M. Delarue

Centre National de la Recherche Scientifique

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Jean‐Claude Boucaut

Centre National de la Recherche Scientifique

Thierry Darribère

Laboratoire de Biologie du Développement

Christian Aimar

Université de Lorraine

Ahmed Haouz

Centre National de la Recherche Scientifique

Kurt E. Johnson

George Washington University

Hélène Munier‐Lehmann

Institut Pasteur

Thomas Ursby

Lund University

Jean‐François Riou

Institut Curie

Dominique Bourgeois

Institut de Biologie Structurale

E. Fioravanti

European Synchrotron Radiation Facility

Cooperative Institutions

Centre National de la Recherche Scientifique

Sorbonne Université

Inserm

Institut Pasteur

Université Paris Cité

Laboratoire de Biologie du Développement

Commissariat à l'Énergie Atomique et aux Énergies Alternatives

Institut de génétique et de biologie moléculaire et cellulaire

Université de Strasbourg

Sorbonne Paris Cité

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Anteroposterior segregation of superficial and deep cells during gastrulation inPleurodeles waltl andRana pipiens embryos

Journal of Experimental Zoology (1996)

M. Delarue Kurt E. Johnson Jean‐Claude Boucaut

Segregation of dorsal superficial and deep cells was analyzed in early Pleurodeles waltl (urodele) and Rana pipiens (anuran) gastrulae in order to gain new comparative insights into anterior and posterior specification of mesodermally fated cells in species other than Xenopus. We used a novel technique of orthotopic transplantation of double-labeled grafts into defined regions. This technique involves labeling all cells in grafts with a rhodamine-lysinated dextran (RLDx) cell lineage tracer (by injecting uncleaved eggs) and subsequently labeling superficial cells with 125I. Small defined grafts of the dorsal marginal zone (DMZ) were placed orthotopically into unlabeled host embryos. RLDx was also used to label individual superficial cells in the early gastrula dorsal midline region as well as large blastomeres in the presumptive DMZ at the 32-cell stage. Labeled progeny were studied in gastrulae and neurulae. The distribution of dorsal mesodermal cells depends on segregation between superficial and deep cells which occurs along the anteroposterior axis during involution in both species studied here. Deep cells are the first to involute. Then they divide into two subpopulations during gastrulation. The first anterior subpopulation migrates with only a few superficial cells. The second, more posterior, subpopulation involutes later and codistributes with most of the superficial cell population. Our results indicate that at the beginning of gastrulation, much greater mixing between superficial and deep cells occurs in Pleurodeles than in Rana. These results may have bearing on studies of the mechanism of anteroposterior specification of amphibian mesoderm. Differences in the timing and topology of expression of cell:cell and cell:substrate adhesion molecules between these amphibian species in early development could account for observed differences in morphogenetic cell movements. © 1996 Wiley-Liss, Inc.

Pleurodeles

Blastoderm

10.1002/(sici)1097-010x(19961201)276:5<345::aid-jez5>3.0.co;2-o

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Citations (7)

Medial cell mixing during axial morphogenesis of the amphibian embryo requires cadherin function

Developmental Dynamics (1998)

M. Delarue Francisco José Sáez Jean‐Claude Boucaut Jean Paul Thiery Florence Broders

A truncated form of Xenopus E-cadherin (ΔE-cad) comprising the cytoplasmic and transmembrane domains was overexpressed generating a dominant negative mutation in the urodelan amphibian embryo Pleurodeles waltl. ΔE-cad mRNA and rhodamine-lysinated-dextran (RLDx) cell lineage tracer were microinjected into 32-cell stage blastomeres which contribute principally to the notochord and central nervous system. ΔE-cad expression causes defects in forebrain and hindbrain formation coupled with the development of supernumerary vesicles. Duplication of the notochord also occurs due to the retardation of medial cell intercalation with correlated duplications of spinal cord and somites. These results emphasize the role of cadherins in mediating cell-cell adhesion in early amphibian embryogenesis. They extend to Pleurodeles the observations made in Xenopus, illustrating that despite differences in morphogenetic processes, the molecular mechanisms are conserved in these two species. Dev. Dyn. 1998;213:248–260. © 1998 Wiley-Liss, Inc.

Notochord

Pleurodeles

Hindbrain

Forebrain

Otic vesicle

Morpholino

10.1002/(sici)1097-0177(199811)213:3<248::aid-aja2>3.3.co;2-6

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Citations (1)

Crystal Structure of Murine Terminal Deoxynucleotidyl Transferase complexed with ddATP

M. Delarue Jean-Baptiste Boulé Julien Lescar Nicole Expert-Bezançon N. Jourdan

Terminal deoxynucleotidyl transferase

Transferase

10.2210/pdb1kej/pdb

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Synthesis and distribution of laminin-related polypeptides in early amphibian embryos

Cell and Tissue Research (1986)

Thierry Darribère Jean‐François Riou Li Shi M. Delarue Jean‐Claude Boucaut

Blastula

10.1007/bf00218997

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Citations (52)

Mycobacterium tuberculosisthymidylate kinase: structural studies of the enzymatic reaction

Acta Crystallographica Section A Foundations of Crystallography (2002)

E. Fioravanti Ahmed Haouz Thomas Ursby Hélène Munier‐Lehmann M. Delarue

Catalase-1 (Cat1) of Neurospora crassa was crystallized by the hanging drop method and a crystal was diffracted at the Stanford Synchrotron Radiation Laboratory.X-ray reflection data were analyzed with denzo, xdisp and CCP4 programs.The crystal belongs to the space group C2, having unit cell parameters of a = 130.007, b = 182.242,c = 90.364Å, β = 133.413°and a dimer in the asymmetric unit.Cat1 structure was determined at 1.75 A resolution using the molecular replacement method and a Escherichia coli catalase (HPII) as starting model.The final R and Rfree values were 18.64 and 20.29, respectively.Cat1 is a large catalase with a C-terminal flavodoxin-like domain, similar in structure to the HPII.Heme in Cat1 was a mixture of protoheme IX (57%) and a heme with two hydroxyl groups in ring D, one of which formed a spirolactone with the propionyl group (43%).HPII also has a heme with a spirolactone.In HPII, the Tyr that coordinates the heme Fe(III) proximally makes a covalent bond with a neighbor His.Interestingly, the equivalent Tyr in Cat-1 makes a covalent bond with a Cys, instead.The corresponding bond being formed between Cys 356 sulfur and Tyr 379 Cb.

10.1107/s0108767302088992

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Amphibian Gastrulation: The Molecular Bases of Mesodermal Cell Migration in Urodele Embryos

Springer eBooks (1991)

Jean‐Claude Boucaut Thierry Darribère De Shi Jean‐François Riou Kurt E. Johnson

Germ layer

Epiblast

10.1007/978-1-4684-6027-8_10

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Citations (8)

Medial cell mixing during axial morphogenesis of the amphibian embryo requires cadherin function