DNA fluorochrome staining with Hoechst 33258 bisbenzimide is commonly used for detection of mycoplasma contamination in cell cultures. Photobleaching of Hoechst 33258 is pronounced under the conditions of intense illumination, high magnification and resolution required for detection of mycoplasmas. To reduce photobleaching we investigated the effects of some antioxidant molecules, p-phenylenediamine (PPD), n-propyl gallate (NPG) and 1,4-diazabicyclo(2,2,2)octane (DABCO), which are known to reduce the fading rate of fluorescein. Mycoplasma-contaminated cell monolayers were stained with Hoechst 33258 and mounted in glycerol containing different amounts of antioxidant additives. The cells were examined in an epifluorescence microscope, and the emitted light intensity was recorded. Results showed that PPD and, to a lower degree, NPG, retarded the photobleaching of Hoechst 33258-stained cells, whereas DABCO was not effective. However, fluorescence half-life was increased about three-fold by NPG and almost 20-fold by PPD. The rate of fluorescence fading of Hoechst 33258 can therefore be retarded by PPD, with obvious advantages for reading and photographic recording of results.
Abstract Objective The purpose of this study was to describe lung ultrasound (LUS) findings at baseline and 48 hours after the beginning of treatment and evaluate how they correlate with outcome Design We prospectively analyzed patients from 1 month to 17 years of age with community acquired pneumonia (CAP) evaluated at a tertiary level pediatric hospital. At baseline and 48 hours after the beginning of treatment, history, clinical examination, laboratory testing, chest X‐ray, and LUS were performed. Results One hundred one children were enrolled in the study (13 with complicated CAP). At baseline those who developed complications presented a larger size of the subpleural pulmonary parenchymal lesions ( P = .001) often associated with a complex pleural effusion (63.6%, P = .013). Those with an uncomplicated CAP presented an air, arboriform, superficial and dynamic bronchogram, as opposed to complicated CAP which had an air and liquid bronchogram, deep, fixed ( P = .001). At the 48‐hour control in the noncomplicated CAP group, bronchogram was more frequently superficial and dynamic ( P = .050). Pleural effusion disappeared in half cases ( P = .050). In all patients, neutrophilic leucocytosis with increased C‐reactive protein was detected and decreased at control ( P = .001). The linear regression analyses showed the switch from a deep to a superficial bronchogram as the only explanatory variable ( r = 0.97, R 2 = 0.94, P = .001, t = 10.73). Conclusions Our study describe early LUS features of CAP that might be able to predict the development of complicated CAP.
Abstract A nosocomial outbreak of rotavirus gastroenteritis involving 52 newborns occurred between June and September 1988 at the University Children's Hospital of Freiburg, Federal Republic of Germany. Stools from 27 representative patients were examined for rotavirus serotypes, using a monoclonal antibody‐based enzyme‐linked immunosorbent assay. The electropherotype was also examined by polyacrylamide gel electrophoresis of genomic RNA. As many as 18 patients were found to be infected by serotype 4, subtype 4B strain, and in all of them the same electropherotype was detected. Although rotavirus from the remaining nine patients could not be typed, the electropherotype in four was identical to that of the serotype 4, subtype 4B strain. Thus, most of the patients in the outbreak were infected by the same rotavirus strain. Retrospective epidemiological studies showed that the 4B strain began to circulate at the hospital in January 1988, whereas only rotavirus serotypes 1, 3, and 4A were detected in 1985–1987. The primary case of the outbreak was presumably a newborn with acute gastroenteritis, admitted to the hospital from a small maternity unit in the same urban area. During the outbreak, 12 of 44 healthy newborns in the nurseries of the Children's Hospital and other maternity hospitals were found to be asymptomatic rotavirus carriers, and in three of the newborns the same 4B strain was detected. This is the first reported outbreak caused by a serotype 4, subtype 4B strain.
El articulo “Evaluacion de mezclas de pulpa de cafe con olote de maiz. Para la produccion de hongos comestibles ( Pleorotus ostreatus )“ es una de las publicaciones tecnicas elaborado por el Programa Cafe y Caffe con el objetivo de beneficiar a los tecnicos, los productores de cafe de calidad y los operadores del sector del cafe.
El Programa Red Regional para el apoyo a las Asociaciones de pequenos productores de cafe de la America Central y Caribe implica seis Paises: Guatemala, Republica Dominicana, El Salvador, Honduras, Costa Rica y Nicaragua.
Este Programa tiene por objetivo mejorar la calidad de vida de los pequenos productores de cafe de las comunidades rurales de Montana y hacer sostenible el cultivo de cafe de calidad.
El Programa cuenta con la financiacion del gobierno de Italia a traves de la Direccion General para la Cooperacion al Desarrollo del Ministerio de Relaciones Exteriores y del Common Fund for Commodities (CFC), que designa como entidad para la supervision tecnica la International Coffee Organization (ICO).
El Istituto Agronomico per l’Oltremare de Florencia (IAO) es la institucion principal responsable de la ejecucion y la coordinacion de las actividades que se realizan en colaboracion con la Fundacion Slow Food para la Biodiversidad e la Ong Italiana Ucodep.
El articulo “Produccion de abono organico por medio del cultivo de lombriz coqueta roja ( Eisenia foetida )” es una de las publicaciones tecnicas elaborado por el Programa Cafe y Caffe con el objetivo de beneficiar a los tecnicos, los productores de cafe de calidad y los operadores del sector del cafe.
El Programa Red Regional para el apoyo a las Asociaciones de pequenos productores de cafe de la America Central y Caribe implica seis Paises: Guatemala, Republica Dominicana, El Salvador, Honduras, Costa Rica y Nicaragua.
Este Programa tiene por objetivo mejorar la calidad de vida de los pequenos productores de cafe de las comunidades rurales de Montana y hacer sostenible el cultivo de cafe de calidad.
El Programa cuenta con la financiacion del gobierno de Italia a traves de la Direccion General para la Cooperacion al Desarrollo del Ministerio de Relaciones Exteriores y del Common Fund for Commodities (CFC), que designa como entidad para la supervision tecnica la International Coffee Organization (ICO).
El Istituto Agronomico per l’Oltremare de Florencia (IAO) es la institucion principal responsable de la ejecucion y la coordinacion de las actividades que se realizan en colaboracion con la Fundacion Slow Food para la Biodiversidad e la Ong Italiana Ucodep.
