Fang Wu

Sichuan Agricultural University

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Duanqing Pei

Westlake University

Jiekai Chen

Chinese Academy of Sciences

Mark E. Bowen

Stony Brook University

Lin Guo

Guangzhou Institutes of Biomedicine and Health

Andrew P. Hutchins

Southern University of Science and Technology

Sujit Basak

GITAM University

Feng Ding

Shenzhen Third People’s Hospital

Xiaoshan Wang

Chinese Academy of Fishery Sciences

George L. Hamilton

Clemson University

Nabanita Saikia

Clemson University

Cooperative Institutions

Chinese Academy of Sciences

Guangzhou Institutes of Biomedicine and Health

Guangzhou Medical University

University of Chinese Academy of Sciences

Hebei Agricultural University

University of Hong Kong

Guangzhou Regenerative Medicine and Health Guangdong Laboratory

Sichuan Agricultural University

Sun Yat-sen University

Southern Medical University

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Cardioprotective effect of Notch signaling on the development of myocardial infarction complicated by diabetes mellitus

Experimental and Therapeutic Medicine (2017)

Fang Wu Bo Yu Xu Zhang Yuelan Zhang

The present study aimed to elucidate the role of Notch signaling in the development of myocardial infarction (MI) concomitant with diabetes in vivo and in vitro and evaluated the therapeutic effect of the Notch signaling in vitro. Streptozotocin-induced diabetic rats were subjected to 25 min of ischemia and 2 h of reperfusion. Cardiac troponin T (cTnT) and creatine kinase-MB (CK-MB) isoenzyme levels were detected. Infarct size was measured by 2,3,5-triphenyltetrazolium chloride staining. Myocardial apoptosis and fibrosis were examined by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling and Masson Trichrome staining, respectively. The mRNA and protein levels of Notch signaling components, including Notch1, Notch4, Delta-like 1, Jagged1, Mastermind-like protein 1 and p300, were quantified by reverse transcription-quantitative polymerase chain reaction and western blotting analyses, respectively. H9c2 cells were treated with/without 33 mM high glucose (HG) and/or subjected to hypoxia in the presence/absence of Jagged1. Cell viability and apoptosis were determined by MTT assay and Annexin V-fluorescein isothiocyanate/propidium iodide assay. Levels of the Notch signaling pathway members were examined. The present findings revealed that diabetes elevated CK-MB and cTnT, increased infarct size, induced myocardial apoptosis and inhibited the Notch signaling pathway in vivo after ischemia/reperfusion. Ischemia/reperfusion augmented the severity of MI in diabetic rats. Furthermore, HG reduced cell viability and induced cell apoptosis in H9c2 cells after hypoxia exposure, which was inhibited by Jagged1. We also found that HG inhibited Notch signaling in H9c2 cells after hypoxia, whereas Jagged1 exerted its cardioprotective effect on hypoxic injury (in HG environments or not) by activating the Notch signaling pathway. In conclusion, these findings suggest that diabetes promoted the progression of MI in vivo and in vitro via the inhibition of the Notch signaling pathway. Jagged1 may protect against MI in in vitro models by activating Notch signaling.

10.3892/etm.2017.4932

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Citations (23)

FGL1 facilitates rather than suppresses anticancer immunity against microsatellite instable gastric cancer

Genes and Immunity (2024)

Zhenyuan Qian Xufan Cai Jianzhang Wu Kun Ke Zai‐yuan Ye

Microsatellite Instability

10.1038/s41435-024-00314-2

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Citations (0)

Fuzzy Supertertiary Interactions within PSD-95 Enable Ligand Binding

Zenodo (CERN European Organization for Nuclear Research) (2022)

George L. Hamilton Nabanita Saikia Sujit Basak Franceine S. Welcome Fang Wu

Data associated with the publication of the same title for single-molecule FRET experiments used to characterize conformational ensembles of the PSG supramodule of PSD-95. Folder 1. Single Molecule Experimental Data .ht3 and .ptu files corresponding to time-tagged photon records collected using single-photon detectors with Hydra Harp TCSPC software and hardware. Folders are provided for each sample. Selection criteria and correction parameters are detailed in the manuscript. DO data were taken from bursts with S_PIE<0.1 while AO data were S_PIE>0.9. 1.1 BG Background files from which background signals were calculated for each sample. 1.2 IRF IRF data files used for generation of IRF curves for fluorescence decay histogram analysis and generation of fFCS filters. Fluorescence Decay Histograms Data files for photon counts in each time bin following donor excitation pulse for Donor-only (DO), FRET (DA), and Instrument Response Function (IRF) curves used in fluorescence decay histogram analysis. Filtered FCS Curves and Filter Files Data corresponding to four fFCS curves per sample: low-FRET-high-FRET (lohi), high-FRET-low-FRET (hilo), low-FRET-low-FRET (lolo), and high-FRET-high-FRET (hihi), as well as filter files generated in the Kristine software package using the .ht3 and .ptu data and IRF files from Single Molecule Experimental Data. Simulation AV-Derived Distance Values Structures from DMD simulations and AV-derived interdye distances used for structural classification against experimental FRET data. Also includes references files for associating distances correctly with structures and FRET pairs.

10.5281/zenodo.6001898

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Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash

PeerJ (2022)

Chuanqiang Xu Fang Wu Jieying Guo Shuan Hou Xiaofang Wu

The plant graft healing process is an intricate development influenced by numerous endogenous and environmental factors. This process involves the histological changes, physiological and biochemical reactions, signal transduction, and hormone exchanges in the grafting junction. Studies have shown that applying exogenous plant growth regulators can effectively promote the graft healing process and improve the quality of grafted plantlets. However, the physiological and molecular mechanism of graft healing formation remains unclear. In our present study, transcriptome changes in the melon and cucurbita genomes were analyzed between control and NAA treatment, and we provided the first view of complex networks to regulate graft healing under exogenous NAA application. The results showed that the exogenous NAA application could accelerate the graft healing process of oriental melon scion grafted onto squash rootstock through histological observation, increase the SOD, POD, PAL, and PPO activities during graft union development and enhance the contents of IAA, GA 3 , and ZR except for the IL stage. The DEGs were identified in the plant hormone signal-transduction, phenylpropanoid biosynthesis, and phenylalanine metabolism through transcriptome analysis of CK vs. NAA at the IL, CA, and VB stage by KEGG pathway enrichment analysis. Moreover, the exogenous NAA application significantly promoted the expression of genes involved in the hormone signal-transduction pathway, ROS scavenging system, and vascular bundle formation.

Squash

Plant hormone

KEGG

Melon

Phenylpropanoid

10.7717/peerj.13980

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Citations (6)

Species-Specific Rewiring of Definitive Endoderm Developmental Gene Activation via Endogenous Retroviruses Through TET1 Mediated Demethylation

SSRN Electronic Journal (2022)

Fang Wu Zhongqi Liufu Yujian Liu Lin Guo Jian Wu

Transposable elements (TEs) are the major sources of lineage-specific genomic innovation and make up nearly half of the human genome, but most of their function remain unclear. Here we identify that a series of endogenous retroviruses (ERVs), a TE subclass, regulate the transcriptome at the definitive endoderm stage by in vitro differentiation model from human embryonic stem cell. Notably, these ERVs perform as enhancer and contain binding sites for critical transcriptional factors for endoderm lineage specification. Genome-wide methylation analysis shows most of these ERVs are induced by TET1 mediate DNA demethylation. LTR6B, a representative definitive endoderm activating ERV, contains binding sites for FOXA2 and GATA4 and governs the primate-specific expression of its neighboring developmental genes such as ERBB4 in definitive endoderm. Together, our study proposes evidence that recently evolved ERVs represent potent de novo developmental regulatory elements, which, in turn, fine-tune species-specific transcriptomes during endoderm and embryonic development.

Endogenous retrovirus

Demethylation

10.2139/ssrn.4099069

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Immunological Anti-Tumor Activity of Icariside I by Regulating Gut Microbiota and Its Derived Metabolites in a Melanoma Mouse Model

Research Square (Research Square) (2020)

Gui Chen Zheng Cao Zunji Shi Hehua Lei Chuan Chen

Abstract Background Emerging evidence has shown that gut microbiome plays important roles in antitumor immunotherapy by shaping systemic immune responses. Specifically, Lactobacillus spp. and Bifidobacterium spp. were previously reported to enhance host immunity and inhibit tumor growth. Furthermore, microbiota-derived metabolites such as short-chain fatty acids (SCFAs) and indole derivatives are gradually regarded as immunomodulatory molecules involved in metabolic signaling pathways. Methods A B16F10 melanoma model was selected as a tumor-bearing animal for the investigation of immunological anti-tumor activity of Icariside I. Gut microbiota structure and composition in cecal content of mice was firstly analyzed by 16S rRNA gene sequence with the goal of screening those bacteria highly associated with tumor immunotherapy. Subsequently, targeted metabolomics approach was employed to quantitatively determine microbiota-derived metabolites such as SCFAs and indole derivatives. The population of multiple lymphocyte subsets in peripheral blood were measured with flowcytometry to evaluate changes of host immunity. Results Icariside I significantly inhibited B16F10 melanoma growth in vivo through regulation of gut microbiota and host immune. Oral administration of icariside I improved the altered gut microbiota community with marked restoration of Lactobacillus spp. and Bifidobacterium spp. abundance. Icariside I was also able to improve the levels of microbiota-derived metabolites such as short-chain fatty acids (SCFAs) and indole derivatives, consequently repairing intestinal barrier and systemic inflammation of tumor-bearing mice. Furthermore, icariside I up-regulated multiple lymphocyte subsets including CD4 + and CD8 + T cells or NK and NKT cells in peripheral blood. Conclusions These results suggested that icariside I may be developed as a novel and natural anticancer adjuvant via microbiome remodeling and host immune regulation.

10.21203/rs.3.rs-66227/v1

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Detection of Distracted Driving Based on MultiGranularity and Middle-Level Features

Min Tang Fang Wu Lili Zhao Qi-Peng Liang Jianwu Lin

A so-called MGMN (Multiple-Granularity- Middle Network) algorithm is proposed to improve the detection accuracy of distracted driving, based on multigranularity features and middle-level features. The algorithm is derived from the ResNet50 neural network and is the first time to use multi-granularity features and mid-level features of images in the field of distracted driving detection. The multigranularity feature extraction module consists of three branches: the global branch to learn the global features without local information, the second branch to divide the image level into two parts and later to learn the local features of the upper and lower parts, and the third branch to divide the image level into three parts, and later to learn the local features of the upper, middle and lower parts. By extracting the features of the middle layer of the image, the feature extraction of the algorithm is enriched. While the multi-granularity features are individually input to the cross-entropy loss function, the multi-granularity features of the image and the middle-level features are combined and input into the cross-entropy loss function. The proposed algorithm has an accuracy of 99.8% on the dataset "Districted-Driver- Detection" published by State Farm Company, which is 1.5% to 3% higher than existing algorithms, and an improved accuracy of 98.7% on the dataset "AUC-Districted-Driver-Detection".

Granularity

Cross entropy

Feature (linguistics)

Middle level

10.1109/cac51589.2020.9327078

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Citations (5)

Salicylic acid treatment delays apricot (Prunus armeniaca L.) fruit softening by inhibiting ethylene biosynthesis and cell wall degradation

Scientia Horticulturae (2022)

Yaling Li Huan He Yuanyuan Hou Abulimiti Kelimu Fang Wu

Pectin

Hemicellulose

10.1016/j.scienta.2022.111061

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Citations (32)

Genome-Wide Characteristics of GH9B Family Members in Melon and Their Expression Profiles under Exogenous Hormone and Far-Red Light Treatment during the Grafting Healing Process

International Journal of Molecular Sciences (2023)

Yulei Zhu Jieying Guo Fang Wu Hanqi Yu Jiahuan Min

β-1,4-glucanase can not only promote the wound healing of grafted seedlings but can also have a positive effect on a plant's cell wall construction. As a critical gene of β-1,4-glucanase, GH9B is involved in cell wall remodeling and intercellular adhesion and plays a vital role in grafting healing. However, the GH9B family members have not yet been characterized for melons. In this study, 18 CmGH9Bs were identified from the melon genome, and these CmGH9Bs were located on 15 chromosomes. Our phylogenetic analysis of these CmGH9B genes and GH9B genes from other species divided them into three clusters. The gene structure and conserved functional domains of CmGH9Bs in different populations differed significantly. However, CmGH9Bs responded to cis elements such as low temperature, exogenous hormones, drought, and injury induction. The expression profiles of CmGH9Bs were different. During the graft healing process of the melon scion grafted onto the squash rootstock, both exogenous naphthyl acetic acid (NAA) and far-red light treatment significantly induced the upregulated expression of CmGH9B14 related to the graft healing process. The results provided a technical possibility for managing the graft healing of melon grafted onto squash by regulating CmGH9B14 expression.

Melon

10.3390/ijms24098258

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Citations (6)

Detect ships using saliency in infrared images with sea-sky background

Proceedings of SPIE, the International Society for Optical Engineering/Proceedings of SPIE (2015)

Fang Wu Chengfei Zhu Wenfang Xue

Nowadays, ship detection in sea-sky background is not only useful in maritime visual surveillance, but also helpful in maritime search and rescue. Since ships are salient objects in infrared images with sea-sky background, we present a novel and effective algorithm based on saliency for ship detection in this situation. Our algorithm adopts global saliency, local saliency and background prior to generate saliency maps. Ships are finally segmented in saliency maps. Our algorithm is compared with four classic salient object detection algorithms. And experimental results show our algorithm outperforms the other four algorithms in qualitative and quantitative terms.

10.1117/12.2197021

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Citations (5)