Objective:Inhibition of alpha-glucosidase and antioxidation of different extracts from the branches and leaves of Taxus chinensis var mairei were studied in vitro to supply the separating base of the effective chemical components.Method:The inhibitory activities of α-glucoside were assayed in external.The antioxidant activities of these extracts were assessed bytwo complementary test systems,namely 1,1-diphenyl-2-picrylhydrazyl(DPPH) radical scavenging activity and ferric reducing antioxidant power(FRAP) assay.Result:The ethyl acetate extract showed strong activity in the inhibitory activities of α-glucoside.(IC50 27.854 1 mg · L-1) The ethanol extract(IC50 29.215 5 mg · L-1)and n-butanol extract(IC50 38.913 0 mg · L-1) displayed lower activity.Petroleum ether fraction(IC50 128.729 5 mg · L-1) and water fraction(IC50 74.505 9 mg · L-1) have a little activity.The antioxidant activities:ethanol extract(DPPH IC50 6.370 7 mg · L-1,FRAP 4.266 mmol · g-1)ethyl acetate extract(DPPH IC50 9.535 8 mg · L-1,FRAP 3.275 mmol · g-1)n-butanol extract(DPPH IC50 20.461 3 mg · L-1,FRAP 1.498 mmol · g-1)water fraction(DPPH IC50 26.685 5 mg · L-1,FRAP 0.678 mmol · g-1)petroleum ether fraction.Conclusion: The components of inhibition α-glucoside could be testified in the ethyl acetate extract and n-butanol extract.Antioxidative components were in the ethyl acetate extract.
Objective To optimize the extraction technology of alginic acid in Laminaria japonica Aresch. Methods The influence of the amount of solvent, the extraction time and temperature (3 factors) on the alginic acid content and yield was investigated by orthogonal test L9(34), and carried on to inspect the methodology. Result The optimum extraction condition was A1B1C2, that was adding 30 times solvent (1%Na2CO3), whisk extracting for one hour at 60 ℃. Conclusion The temperature is the most important factor. The content of alginic acid decrease obviously at above 60 ℃. The optimum extraction technology is reasonable.
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