Haibin Wang

Nanjing Agricultural University

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Clifford J. Woolf

Boston Children's Hospital

Jingfang Fan

Potsdam Institute for Climate Impact Research

Li‐Jun Duan

Ningbo University

Yuling Wang

Hebei Medical University

Sudhansu K. Dey

University of Science and Technology Chittagong

Huanwei Zheng

Jining Traditional Chinese Medicine Hospital

Sumei Chen

Ministry of Agriculture and Rural Affairs

Fumimasa Amaya

Kyoto Prefectural University of Medicine

Shengbo Zhang

Inner Mongolia University

Jianhua Lu

Qidong Liver Cancer Prevention Research

Cooperative Institutions

Nanjing Agricultural University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Sciences

Hebei Medical University

China Agricultural University

Peking University

Shanghai Jiao Tong University

Chinese Academy of Medical Sciences & Peking Union Medical College

Shandong First Medical University

Capital Medical University

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Protective effects of Purendan superfine powder on retinal neuron apoptosis in a rat model of type 2 diabetes mellitus.

PubMed (2012)

Zhijun Dong Xiang-yi Tao Xiaoxiao Fu Haibin Wang Donghua Wang

This study sought to investigate the effects of Purendan superfine powder comprised of Momordica charantia, Radix Ginseng, and Radix Salviae Miltiorrhiae on neuronal apoptosis and expression of bcl-2, bax, and caspase-3, which are retinal apoptosis-associated factors in rats with diabetes mellitus induced by continuous intraperitoneal injection of streptozotocin. The results showed that Purendan superfine powder could upregulate the expression of bcl-2 protein and mRNA, and downregulate the expression of bax and caspase-3 in the retina of diabetes mellitus rats. In addition, Purendan superfine powder was shown to reduce the number of apoptotic neurons. Our experimental findings indicate that Purendan superfine powder can inhibit neuronal apoptosis in the retina of diabetes mellitus rats and has protective effects on diabetic retinopathy.

Intraperitoneal injection

10.3969/j.issn.1673-5374.2012.03.008

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Citations (5)

[Effect of vascular endothelial growth factor 165 gene transfection on repair of bone defect: experiment with rabbits].

PubMed (2007)

Dongmei Zhao Jia-feng Yang Shiqing Wu Liping Qiu Jun‐Li Liu

To investigate the influence of vascular endothelial growth factor (VEGF) 165 gene transfection on the repair of bone defect.38 New Zealand rabbits underwent resection of a segment 1 cm in length in bilateral radii filled with absorbable gelatin sponge. Dilated solution of the plasmid pcDNA3.1/VEGF165 was injected into the bone defect of one side and normal saline was injected into the contralateral bone defect. 1, 2, 4, 6, 8, and 12 weeks later X ray examination was conducted to observe the repair of bone defect, and then 5 rabbits were killed at each time points to take out the bone defects. HE staining was used to observe the bone repair. The levels of microvessel density (MVD) 1 and 2 weeks after the operation were observed. RT-PCR was used to detect the mRNA expression of VEGF in the bone defect. Based on the results of RT-PCR the tissue mRNA expression of VEGF65 was detected by real-time quantitative polymerase chain reaction (RQ-PCR).X-ray examination showed that there was no significant difference in the wound healing between the two group 1 week after the operation in all rabbits. Some callus could be seen in the experimental group 2 weeks after. Twelve weeks after the operation the reconstruction of bone cortex was completed. Similar process occurred in the control sides but more lately. The MVD level 7 days after of the experimental group was 47.0 +/- 7.5, significantly higher than that of the control group (42.2 +/- 6.4, t = 2.4519, P = 0.0179), and the MVD level 14 days after of the experiment group was 69.1 +/- 5.4, significantly higher than that of the control group (56.1 +/- 6.1, t = 8.0347, P = 0.0000). In the experimental group the mRNA expression amounts of VEGF165 could be found 1 week after, gradually increased and peaked 3 weeks after, then decreased, and became stable 6 weeks after. The mRNA expression amounts of VEGF165 in the control group were lower than those of the experimental group.Local application of PcDNA3.1/VEGF(165) vector promotes the expression of VEGF165, and enhances the quantity of the angiogenesis, extra cellular matrix and healing of bone defect.

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Citations (3)

Single-cell immune profiling reveals distinct immune response in asymptomatic COVID-19 patients

Signal Transduction and Targeted Therapy (2021)

Xiang-Na Zhao Yue You Xiaoming Cui Huixia Gao Guolin Wang

Abstract While some individuals infected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) present mild-to-severe disease, many SARS-CoV-2-infected individuals are asymptomatic. We sought to identify the distinction of immune response between asymptomatic and moderate patients. We performed single-cell transcriptome and T-cell/B-cell receptor (TCR/BCR) sequencing in 37 longitudinal collected peripheral blood mononuclear cell samples from asymptomatic, moderate, and severe patients with healthy controls. Asymptomatic patients displayed increased CD56 bri CD16 − natural killer (NK) cells and upregulation of interferon-gamma in effector CD4 + and CD8 + T cells and NK cells. They showed more robust TCR clonal expansion, especially in effector CD4 + T cells, but lack strong BCR clonal expansion compared to moderate patients. Moreover, asymptomatic patients have lower interferon-stimulated genes (ISGs) expression in general but large interpatient variability, whereas moderate patients showed various magnitude and temporal dynamics of the ISGs expression across multiple cell populations but lower than a patient with severe disease. Our data provide evidence of different immune signatures to SARS-CoV-2 in asymptomatic infections.

Profiling (computer programming)

2019-20 coronavirus outbreak

10.1038/s41392-021-00753-7

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Citations (43)

Extraction of Melanin in Apricot Kernel Shell and the Optimization of Chelating Process of Its Metal Chelates

DOAJ (DOAJ: Directory of Open Access Journals) (2022)

Li Gao Linlin Liu Panpan Liu Hongyu Xu Haibin Wang

Kernel (algebra)

10.13386/j.issn1002-0306.2021100255

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Clinical Study on Treatment to Ankylosing Spondylitis with Fengshi Qutong Capsule-Diclofenac Sodium Combination

Journal of Clinical and Nursing Research (2021)

Haibin Wang Ying Wang Xin Tian Shizhang Liu Le Ji

Objective: To discuss the clinical study on Fengshi Qutong capsules combined with diclofenac sodium in the treatment of ankylosing spondylitis. Methods: 80 patients who were treated for ankylosing spondylitis from June 2019 to June 2020 were selected and divided into two groups. The experimental group was treated with Fengshi Qutong capsules combined with diclofenac sodium, and the control group was treated with sulfasalazine enteric-coated tablets. Results: The treatment efficacy, VAS score, BASDAI score, BASFI score, CRP level, TNF-? level, IL-I? level, and the incidence of adverse reactions between the two groups were significantly different (P<0.05). Conclusion: The application of Fengshi Qutong capsules combined with diclofenac sodium in the treatment of patients with ankylosing spondylitis is beneficial to improve the treatment efficacy, reduce the levels of CRP, TNF-?, and IL-I?, reduce the incidence of adverse reactions, and reduce the VAS, BASDAI and BASFI scores, rendering it of important clinical value.

BASFI

Diclofenac Sodium

Diclofenac

Spondylitis

10.26689/jcnr.v5i3.2002

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Longitudinal single-cell immune profiling revealed distinct innate immune response in asymptomatic COVID-19 patients

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Xiang-Na Zhao Yue You Guolin Wang Huixia Gao Xiao-Ming Cui

SUMMARY Recent studies have characterized the single-cell immune landscape of host immune response of coronavirus disease 2019 (COVID-19), specifically focus on the severe condition. However, the immune response in mild or even asymptomatic patients remains unclear. Here, we performed longitudinal single-cell transcriptome sequencing and T cell/B cell receptor sequencing on 3 healthy donors and 10 COVID-19 patients with asymptomatic, moderate, and severe conditions. We found asymptomatic patients displayed distinct innate immune responses, including increased CD56 bri CD16 − NK subset, which was nearly missing in severe condition and enrichment of a new Th2-like cell type/state expressing a ciliated cell marker. Unlike that in moderate condition, asymptomatic patients lacked clonal expansion of effector CD8 + T cells but had a robust effector CD4 + T cell clonal expansion, coincide with previously detected SARS-CoV-2-reactive CD4 + T cells in unexposed individuals. Moreover, NK and effector T cells in asymptomatic patients have upregulated cytokine related genes, such as IFNG and XCL2 . Our data suggest early innate immune response and type I immunity may contribute to the asymptomatic phenotype in COVID-19 disease, which could in turn deepen our understanding of severe COVID-19 and guide early prediction and therapeutics.

CD16

10.1101/2020.09.02.276865

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Citations (9)

The Heterologous Expression of a Chrysanthemum nankingense TCP Transcription Factor Blocks Cell Division in Yeast and Arabidopsis thaliana

International Journal of Molecular Sciences (2019)

Xiangyu Qi Yixin Qu Ri Gao Jiafu Jiang Weimin Fang

Both the presence of, and the important contribution to growth and development made by TCP transcription factors, have been established in various plant species. Here, a TCP4 homolog isolated from Chrysanthemum nankingense was shown to be more strongly transcribed in the diploid than in the autotetraploid form of the species. CnTCP4 was shown to encode a member of the class II TCP family and to be transcribed most strongly in the leaf and ligulate flowers. Its transcription was found to be substantially inhibited by spraying the plant with the synthetic cytokinin 6-benzylaminopurine. The transient expression of CnTCP4 in onion epidermal cells showed that its product localized to the nucleus, and a yeast one hybrid assay suggested that its product had transcriptional activation ability. The constitutive expression of CnTCP4 in fission yeast suppressed cell proliferation, inducing the formation of longer and a higher frequency of multinuclated cells. Its constitutive expression in Arabidopsis thaliana reduced the size of the leaves. The presence of the transgene altered the transcription of a number of cell division-related genes. A yeast one hybrid assay identified a second TCP gene (CnTCP2) able to interact with the CnTCP4 promoter. A transient expression experiment in Nicotiana benthamiana leaves showed that CnTCP2 was able to activate the CnTCP4 promoter. Like CnTCP4, CnTCP2 was shown to encode a member of the class II TCP family, to be transcribed most strongly in the leaf and ligulate flowers, and to be suppressed by exogenous 6-benzylaminopurine treatment. The CnTCP2 protein also localized to the nucleus, but had no transcriptional activation ability. Its constitutive expression in A. thaliana had similar phenotypic consequences to those induced by CnTCP4.

Transcription

10.3390/ijms20194848

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Citations (10)

The Charcot–Leyden crystal protein revisited—A lysopalmitoylphospholipase and more

Journal of Leukocyte Biology (2020)

Peter F. Weller Haibin Wang Rossana C. N. Melo

Abstract The Charcot–Leyden crystal protein (CLC-P), a constituent of human and not mouse eosinophils, is one of the most abundant proteins within human eosinophils. It has a propensity to form crystalline structures, Charcot–Leyden crystals, which are hallmarks in their distinctive extracellular crystalline forms as markers of eosinophilic inflammation. The functions of CLC-P within eosinophils have been uncertain. Although the action of CLC-P as a lysophospholipase has been questioned, assays of chromatographically purified CLC-P and crystal-derived CLC-P as well as studies of transfected recombinant CLC-P have consistently documented that CLC-P endogenously expresses lysophospholipase activity, releasing free palmitate from substrate lysopalmitoylphosphatidylcholine. Rather than acting solely as a hydrolytic enzyme to release palmitate from a lysolipid substrate, some other lysophospholipases function more dominantly as acyl-protein thioesterases (APTs), enzymes that catalyze the removal of thioester-linked, long chain fatty acids, such as palmitate, from cysteine residues of proteins. As such APTs participate in palmitoylation, a post-translational modification that can affect membrane localization, vesicular transport, and secretion. CLC-P has attributes of an APT. Thus, whereas CLC-P expresses inherent lysophospholipase activity, like some other lysophospholipase enzymes, it likely also functions in regulating the dynamic palmitoylation cycle, including, given its dominant subplasmalemmal location, at the human eosinophil’s plasma membrane.

Lysophospholipase

Palmitoylation

Lipid-anchored protein

Thioester

10.1002/jlb.3mr0320-319rr

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Citations (15)

Zymosan-induced glycerylprostaglandin and prostaglandin synthesis in resident peritoneal macrophages: roles of cyclo-oxygenase-1 and -2

Biochemical Journal (2006)

Carol A. Rouzer Susanne Tranguch Haibin Wang Hao Zhang Sudhansu K. Dey

COX [cyclo-oxygenase; PG (prostaglandin) G/H synthase] oxygenates AA (arachidonic acid) and 2-AG (2-arachidonylglycerol) to endoperoxides that are converted into PGs and PG-Gs (glycerylprostaglandins) respectively. In vitro, 2-AG is a selective substrate for COX-2, but in zymosan-stimulated peritoneal macrophages, PG-G synthesis is not sensitive to selective COX-2 inhibition. This suggests that COX-1 oxygenates 2-AG, so studies were carried out to identify enzymes involved in zymosan-dependent PG-G and PG synthesis. When macrophages from COX-1−/− or COX-2−/− mice were treated with zymosan, 20–25% and 10–15% of the PG and PG-G synthesis observed in wild-type cells respectively was COX-2 dependent. When exogenous AA and 2-AG were supplied to COX-2−/− macrophages, PG and PG-G synthesis was reduced as compared with wild-type cells. In contrast, when exogenous substrates were provided to COX-1−/− macrophages, PG-G but not PG synthesis was reduced. Product synthesis also was evaluated in macrophages from cPLA2α (cytosolic phospholipase A2α)−/− mice, in which zymosan-induced PG synthesis was markedly reduced, and PG-G synthesis was increased approx. 2-fold. These studies confirm that peritoneal macrophages synthesize PG-Gs in response to zymosan, but that this process is primarily COX-1-dependent, as is the synthesis of PGs. They also indicate that the 2-AG and AA used for PG-G and PG synthesis respectively are derived from independent pathways.

Zymosan

10.1042/bj20060615

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Citations (27)

Spatiotemporal expression of endogenous opioid processing enzymes in mouse uterus at peri-implantation

Cell and Tissue Research (2015)

Weiwei Wu Shuangbo Kong Bingyan Wang Yongjie Chen Haibin Wang

Endogenous opioid

10.1007/s00441-015-2259-8

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Citations (1)