Objective To evaluate on measuring vaginal sialidase activity to identify bacterial vaginosis(BV) by using 5-bromo-4-chloro-3-indolyl-alpha-D-N-acetylneuraminic acid(X-Neu5Ac) as the substrate.Methods The study population comprised 45 of them with clinical signs of BV(BV+) and 30 of them without BV(BV-).Bacterial vaginosis was diagnosed by clinical criteria.The vaginal fluids from suspected patients were evaluated by sialidase levels and collected for quantitative determination,including Lactobacillus spp,Bacteroides spp,Escherichia coli,Staphylococcus spp,Streptococcus spp,Gardnerella vaginalis.Sialidase activity using X-Neu5Ac as the substrate,specific activity was expressed as nanomoles of methoxyphenol produced.The method of measuring vaginal sialidase activity was compared to the standard method for diagnosing BV(Amsel criteria).Results The sensitivity and specificity of the test for prediction of BV were 88.9% and 90%,respectively,with no significant differences(P0.05) from those by Amsel criteria.Positive and negative predictive values were 93% and 84.3%,respectively.The mean Gardnerella vaginalis count in the sialidase-positive group was 6.95 log colony-forming units(CFU)/g,which was significantly higher(P0.01) than that(2.05 log CFU/g) in the sialidase-negative group.The mean count of hydrogen peroxide-producing Lactobacilli(LB+) species was significantly higher(P0.05) in the sialidase-negative group than in the positive counterpart(4.26 vs 8.66).In contrast,there was no difference in the mean total aerobe count between the two groups,including Staphylococcus spp,Enterococcus spp and Escherichia coli.Conclusions The vaginal sialidase activity for identifing bacterial vaginosis(BV) by using X-Neu5Ac as the substrate was a useful diagnostic tool of BV.Elevation of vaginal sialidase activity,an increase in the anaerobe count,and decreases in LB+ count could be good predictors of the deterioration of bacterial vaginosis.
A new fluorescence quenching method for the determination of trace mercury has been established.This method is based on the facts that 2,3-dihydro-9,10-dihydroxy-1,4-anthraquinone(R)can emit strong and stable fluorescence in HCl solution with α,α'-dipyridine,and α,α'-dipyridine can activate Hg~(2+) catalyzing the reaction of H_(2)O_(2)oxidizing R to non-fluorescence complex which cause the fluorescence quenching.The regression equation of working curve can be expressed as △I_(f) = 10.19 + 199.9 C _(Hg2+)(ng/mL)(r = 0.9994,n =5).The limit detection(LD) is 7.2 × 10~(-13)g Hg~(2+)/mL(n=11).The sample containing 0.0040 and 0.40ng/ml Hg~(2+) are repeatedly determined for 11 times.RSD are 2.2% and 3.4% respectively.R is synthesized in this paper.Meanwhile,the structure is determined by ~(1)H-NMR,IR~(),MS and UV-Vis spectroscopy.
Objective To produce the mouse models of vaginal microflora dysbiosis induced by antibiotic,and study the adjustment of Lactobacillus on vaginal infection.Method 50 mg/d ceftriaxone was given to the IRC mice intragastrically for 5 days and inoculated intra-vaginally with 20 μl of a suspension of Candida albicans to make mouse vaginal candidias albican imbalance model.The model mice were then inoculated intra-vaginally with 20 μl of a suspension of 2×108CFU/ml Lactobacillus.After treatment the Candida albicans and Lactobacillus were quantified by culture of vaginal lavage fluid and Papanicolaou staining of vaginal tissue.Result The amounts of Lactobacillus in the vaginal of model group decreased significantly(P0.01).White blood cells and Candida albicans were found through vaginal secretion examination by microscopy.After treatment,the amount of Lactobacillus in the vagina of the mice treated by Lactobacillus increased and recovered to normal level(P0.05),while the amount of Candida albicans decreased significantly.We also found that the rebuilt viginal microbiota could exclude Candida albicans dramatically,which indicated that the colonization resistance of the flora was recovered(P0.01).Interestingly,all of the efficiency was impressed with the Lactobacilus pre-treated group.Conclusion The adhesion of Candidias albican to vaginal mucous membrane could be prevented by Lactobacilus imported from outside the vagina.The vaginal microecosystem could be adjusted and the vaginal mucous membrane could be mended by Lactobacilus.
Objective To clone the functional domains of Streptococcus mutans Glucan-Binding Protein B(GbpB) and express it′s fusion protein in Lactoboccus lactis.Method First,the GbpB gene of Streptococcus mutans was cloned into the prokaryotic espressive vector pNI1.Second,the recombinant vector GbpB-pNI1 was transformated into Lactoboccus lactis strain YF02 to express GbpB protein.Then the recombinant GbpB was induced to express and was identified by SDS-PAGE.Result The functional domains of Glucan-Binding Protein B was cloned correctly and it′s fusion protein was expressed in Lactoboccus lactis.Conclusion PCR(polymerase chain reaction) and construction of fusion protein technique are effective methods to get the aimed genes and it′s fusion protein.This experiment has provided a base to the further research.
The South-East Asian Journal of Medical Education (SEAJME) is the culmination of the SEARAME's vision to bring together medical educationists in the region to promote collaborative efforts toward uplifting the standards of medical education in the region and beyond through dissemination of knowledge. This journal will be the ideal platform to disseminate research findings and stimulate discussion among experts in the field. Open access policy of the journal will facilitate this ideal.From November 2019, The South-East Asian Journal of Medical Education (SEAJME) is indexed in EuroPub
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