A 60-day feeding trial was conducted in a static indoor rearing system in 12 glass aquaria to investigate the possibility of using soybean meal protein in lieu of fishmeal as dietary protein source for Labeo rohita fry. Four iso-nitrogenous (30% protein) and iso-caloric (19 kJ/g GE) experimental diets were prepared as: diet 1 (100% fishmeal protein), diet 2 (75% fishmeal protein + 25% soybean meal protein), diet 3 (50% fishmeal protein + 50% soybean meal protein) and diet 4 (25% fishmeal protein + 75% soybean meal protein) and were fed to triplicate groups of 10 fish (per aquarium). Fish were fed two times daily at a rate of 5% of their body weight during the entire experimental period. The feeding ration was adjusted every 10th day through sampling of fish. Growth rate, feed conversion ratio (FCR) and protein efficiency ratio (PER) were significantly higher (P<0.05) in fish fed with diet 2, but those were similar to the diets 1 and 3. Analysis of proximate composition of the whole fish fed with different diets did not show any significant difference (P>0.05). The growth rates, FCR and PER as obtained from the different treatments indicate that fishmeal protein could be replaced up to 50% by soybean meal protein in the diet of L. rohita fry without supplementation of amino acids. DOI: http://dx.doi.org/10.3329/agric.v10i2.13143 The Agriculturists 2012; 10(2) 68-76
Histopathology is promising field for research in aquatic toxicology as it provides the real picture of the toxic effects of xenobiotics in vital functions of a living organism. The present study aims to evaluate the toxic effect of pesticide namely profenofos on silver barb. Liver and kidney of silver barb were examined histologically after exposure to sublethal concentrations (0.01 ppm, 10% of LC50 and 0.05 ppm, 50% of LC50) of profenofos for 0, 7, 15 and 30 days. Histological recovery was also studied by maintaining the pesticide‐exposed fish in a freshwater system for an additional 7, 15 and 30 day. Kidney and liver of exposed individuals exhibited some remarkable changes in their histology in comparison to control and recovery group. Hepatic lesions in the liver tissues of fish were characterized by cloudy swelling of hepatocytes, lipoid vacuoles, pycnotic nuclei and focal necrosis. Epithelial hypertrophy, narrowing of the tubular lumen, atrophy of the glomerulus, broader Bowman's capsule, necrosis in the epithelial cells and pycnosis in the hematopoietic tissue were observed in kidney tissues of experimental fish. These lesions grew with increasing concentration. Although some of the changes were reversible, the rest were less pronounced after a recovery period.Progressive Agriculture 28 (3): 240-248, 2017
Studies on Gonadosomatic index (GSI), ova diameter and histology of the gonad were performed to understand reproductive biology of Feather back, Chital (Notopterus chitala) for a period of 6 months from January to June 2010. Thirty live chital fish were used in this study. The mean GSI values for female chital were found to range between 0.20±0.013 and 4.63±0.50. The highest GSI value was found in June. The smallest diameter of ovum was recorded 0.04 mm (January) and the largest was 4.00 mm (June). During the experimental period, the fecundity was ranged from 8,238 to 18,569 (mean 13,052±4607) in fish samples with body weight range from 1,296 to 2,360 (mean 1,742.50±474.44 g) while the relative fecundity was 5.65 to 14.33. Histological study revealed that the percentage of late perinucleolus (LPN) stage was highest in April and Cortical alveoli (CA) stage appeared from April and reached to maximum in May. Vitellogenic stage (VG) appeared in the month of May and chronologically increased through June. Vitellogenic stage (VG) of oocyte as well as highest ova diameter reached to the peak in June. The variations in the gonad weight and GSI of the female fish reached to the peak during June indicating maturity of ovary and definite spawning season. DOI: http://dx.doi.org/10.3329/ijarit.v2i1.13991 Int. J. Agril. Res. Innov. & Tech. 2 (1): 26-31, June, 2012
Production performance of two tilapia (Oreochromis niloticus) strain viz. Chitralada and GIFT (Genetically Improved Farmed Tilapia) in fish-prawn polyculture system was compared, and possible effect of tilapia inclusion in freshwater prawn (Macrobrachium rosenbergii) culture was evaluated in 120 days of grow-out phase. There were three treatments, i.e. only freshwater prawn as control (T1), prawn + Chitralada (T2), and prawn + GIFT (T3) with three replications of each. Stocking densities for prawn juvenile was 30,000 ha-1 in all the treatments and for both tilapia strains were 10,000 ha-1. Floating feed containing 28% protein for tilapia and pelleted sinking feed for prawn were provided twice daily at a rate of 10-5% bw adjusted after each month of sampling. Water transparency was significantly lower (P<0.05) in treatment T1 with significantly higher chlorophyll-? content that increased with progression of time. Along with, plankton abundance was significantly higher (P<0.05) in T1 indicating prawn-tilapia mixed culture is better than traditional single species culture of prawn in terms of water quality management. Average individual weight, final weight, survival and production of prawn did not differ significantly (P>0.05) among the treatments. In case of tilapia, average individual weight was significantly higher in Chitralada (254.22 g) than that of GIFT (201.52 g). However, survival of GIFT was significantly higher (92.50%) than that of Chitralada (65.83%). Gross and net production of tilapia did not vary significantly. It was concluded that prawn-tilapia mixed culture is advantageous over traditional mono culture regarding production augmentation, and tilapia inclusion improving water quality.J. Bangladesh Agril. Univ. 14(1): 127-134, June 2016
An on-farm experiment was undertaken in nine earthen ponds to evaluate the growth and production potentials of stinging catfish shing, Heteropneustes fossilis for the period of six months from March to August 2010. Three stocking densities such as 1,25,000 (T1), 1,87,500 (T2) and 2,50,000 ha-1 (T3) were tested with three replications each. Fish were fed with commercial pelleted feed containing 35% crude protein. After six months rearing, the mean harvesting weights of shing were 69.42±6.20, 58.74±3.85 and 49.50±4.52g in T1, T2 and T3, respectively. Significant (P<0.05) highest mean harvesting weight was found in T1. The best survival was found in T1 (87%) among the treatments. The calculated mean production of shing (H. fossilis) in three treatments such as T1, T2 and T3 were 7549±52, 9031±71 and 8786±60 kg ha-1, respectively, which were significantly different (p<0.05) from each other. DOI: http://dx.doi.org/10.3329/ijarit.v2i2.14009 Int. J. Agril. Res. Innov. & Tech. 2 (2): 9-14 December, 2012
The study was performed for evaluating the growth and production performances of crossbred climbing perch (Anabas testudineus) during August to October 2009. The crossbred groups of fish were produced by crossing between native climbing perch and Thai climbing perch (A. testudineus). Growth and production performances was compared at grow out condition between crossbred groups in the two reciprocal crosses of treatments viz. T1: Native climbing perch (?) × Thai climbing perch (?) and T2: Thai climbing perch (?)×Native climbing perch (?. At the same time, they were compared also with their respective control F1 pure line groups i.e. T3: Native climbing perch (?) × Native climbing perch (?) and T4: Thai climbing perch (?) × Thai climbing perch (?). It was evident from the results that among the treatments in grow out system, the highest mean harvesting weight was 69.25±9.01 g found in T4 (Thai climbing perch), which was significantly different (P<0.05) from all other treatments. Whereas, T3 (native climbing perch) also showed the lowest mean harvesting weight 33.38±8.74 g and showed significant differences (P<0.05) among all the treatments. The mean harvesting weight of both the crossbred groups i.e. T1 and T2 were 50.83±6.65 and 59.94±7.83 g, respectively and these results were statistically insignificant (P>0.05) between them but significant among all other treatments. The highest (93.60%) and lowest (83.06%) survival rates were observed in Thai koi (T4) and native Koi (T3) parental stocks, respectively. Wider variations in terms of productions performances were observed among the treatments, where the gross production of T1, T2, T3 and T4 were 2,753±96.67, 3,401±73.26, 1,733±168.47 and 4,051±185 kg/ha, respectively. The productions obtained from crossbred groups were at intermediate compared to their control pure line groups. Higher FCR mean value was observed in native control line (T3), while, lower FCR mean value was observed in Thai control line (T4). DOI: http://dx.doi.org/10.3329/ijarit.v2i1.13990 Int. J. Agril. Res. Innov. & Tech. 2 (1): 19-25, June, 2012
