ObjectiveTo measure the expression level of T Lymphocyte subsets in peripheral blood in order to investigate the change of immunity in children with infectious mononucleosis(IM).T Lymphocyte subsets of peripheral blood were measured by flow cytometry in 37 cases with IM. They were compared with 20 normal children.The IM patients showed an obvious decrease in percentage of CD 4 cells as well as ratio of CD 4 +/CD 8 +, but obvious elevation of CD 3、CD 8 cells.[Conclusion]Abnormal immunity resulted from Epstein-Barr virus(EBV) infection plays an important part in children with IM.
Double GCRV outer capsid protein VP7 genes(0.9 kb)and β-actin promoter(0.56 kb)from Megalobrama amblycephala were amplified and cloned into gene transfer vector pFastBacTM Dual to obtain recombinant pFastBac-β-VP71-VP72.Immune experiment and immunological effect were studied.The recombinant plasmids were divided into three groups based on doses:10,30,60 μg.30 μg pFastBacTM Dual group and control group were designed in addition.Immune efficacy was determined by means of transcriptions of VP7,antibody detection by indirect agglutination reaction and challenge experiment on the 14th,21st,28th,and 49th days after immunization.The results showed that pFastBac-β-VP71-VP72 was transformed into body cell,VP7 gene could be controlled for expression constantly by β-actin promoter,and transcription of VP7 was still in process on the 49th day.Antibody was produced and the titers reached their peak on the 21th day after immunization.The mortality rates were 0%,0% and 5% respectively in 10 μg,30 μg and 60 μg vaccine immunization groups,30% in pFastBacTM Dual group,and 100% in control group after GCRV injection.All results suggested that pFastBac-β-VP71-VP72 had good immuno-protective efficacy as DNA vaccine against GCRV.Therefore,the study provided important academic basis for research,development and application of the GCRV genetic vaccine.
Objective To study the occurrence and distribution of mumps in primary school in Shenzhen.Methods Serum samples were collected from th ree primary schools(including one private school)according to the cross-sectional sampling method.Mumps specific IgG and IgM antibodies were detected by ELISA.Results We examined477school children and the results s howed that the total mumps IgG antibody positive rate was 78.2%with avera ge antiboby level of 489.8U /ml.The total mumps I gM antibody positive rate was 3.1%.I gG antibody positive rate of the perm anent resident school children was 81.3%w ith average antiboby level of 426.0U /ml.IgG antibody positive rate of te mporary resident school children was 71.8%w ith average antibody level of 621.2U/ml.There is a significant differen ce between two groups(χ 2 =5.587,P0.05).There is no significant different i n the antibody level in different age groups (χ 2 =7.155,P0.05).Conclusions The immunity against mumps was considerably high in the primary school ch ildren of Shenzhen,but antibody level in the p ermanent resident school children i s lower than the temporary resident s chool children.It is necessary to implement vaccination program to enhance the immunity level.
Freedom in love affairs and marital relationship,as it is,has been considered as of great significant in modern Chinese enlightenment movement in May-Fourth liberation discourse.Nevertheless,as for such freedom advocated and defined merely by the male,has it become a safe channel for the male to express his own sexual desires? Or is it just the male's wishful thinking to help the female to speak out their sexual depression? Reading through all the love novels written by the male writers during the May-fourth period,we have found out that the male writers tend to rampantly let out their sexual desire under the disguise of female liberation accepted and supported by the female.Hence,we conclude that the male' s liberation of sexual desire is the real reason for the female's tragedy,because the female's ideal for romantic love has been buried by the male's immoderate sexual desires.
Objective To investigate the changes of NGF mRNA expression and the effect of exogenous interleukin 1β(IL 1β) on it in rats after traumatic brain injury(TBI) and to explore the mechanism of NGF and IL 1β in TBI. Methods A brain trauma model of fluid percussion in rats was established. Changes of NGF mRNA expression and effect of exogenous IL 1β on it were observed by RT PCR, molecular hybridization and immunocytochemical techniques. Results NGF mRNA expression in the brain injury site and tissues adjacent to it began to increase at 12 h after trauma and increased markedly at 24 h and reached the peak value on the 3rd. Then it decreased gradually, but still higher than that of the control. NGF mRNA expression increased at 3 h after trauma in IL 1β treatment group and was significantly higher than that in simple trauma and control groups( P 0.01) and peaked at 24 h following brain trauma. Conclusion High expression of NGF mRNA after TBI may be related to the mechanisms of neuron protection and repair. Exogenous IL 1β can increase NGF mRNA expression level following brain trauma.
Objective Fibrinolytic enzymes(FLE) which wildly exist in many kinds of snake venoms as a kind of novel powerful thrombotic agent can directly lysis fibrin or fibrinogen.It has the highest value in anti-thrombotic diseases.Methods:The FLE sequence,cloned from Gloydius intermedius venom gland,which was cutted by double enzyme digestion from pMD18T-FLE plasmid was subcloned to pFASTBACHTa eukaryotic expressive vector.Recombinant eukaryotic expressive plasmid,pFASTBACHTa-FLE,was constructed after transformation,selection and identification.The recombinant plasmid was injected into mice in large quantity and large volume in a short time through tail vein.Conclusion: Recombinant FLE was examined in liver tissue by SDS-PAGE and Western blot.Immunohistochemistry showed that there were a great of recombinant FLE proteins could be found in liver tissue.The fibrinolytic activity of recombinant protein revealed by the modified fibrin plate method demonstrated that it had efficiently fibrinolytic activity to be dose-dependent and time-relative.Therefore,a solid foundation for using snake venom FLE from Gloydius intermedius was built.
Objective:Identification of the antigenic determinants of hALR.Methods:Theoretic antigenic determinants of hALR was predicted by using HoppWodds method and and Goldkey software package.The four polypeptides according to the amino acid sequences of the predictive linear epitopes of hALR were synthesized and were used to analyze the antigenic determinants of hALR recognized by antibodies.Results:The polypeptides corresponding to residues 6～5,68～80 and 105～112 of hALR were its antigenic determinants.Conclusion:Prediction of the protein antigenic determinants by computer program and detection of them by competitive ELISA with synthesized polypeptides is a useful method of identification of the antigenic determinants.
This study aimed to evaluate the immunological and the protective efficacy of the yeast recombinant hepatitis B vaccine(YRV)(China made)in clinical trials. 166 newborns whose mothers were HBsAg and/or HBeAg positive and 491 infants whose mothers had no HBV M were vaccinated with YRV of 5μg each dose by 0 1 6 or 0 1 2 month schedules. The YRV was proven safe and no serious side effect. The anti-HBs positive seroconversion rates in 0 1 6 group and 0 1 2 group(both were born from HBV M negative mothers)were 92.79% and 91.04% respectively at 6th month after three doses. The anti HBs positive seroconversion rates of infants whose mothers were HBsAg and/or HBe Ag positive in 0 1 6 group and 0 1 2 group were 82.97% and 81.94%, respectively. The rates of protective efficacy in 0-1-6 and 0-1-2 groups were 81.08% and 81.48% respectively. The results demonstrate the better immunogenicity and higher protective efficacy of China made YRV.
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