Infectious Bursal Disease (IBD) is an important disease of young chickens having significant economic implications for poultry industry worldwide. The exact target molecule for attachment of IBD virus (IBDV) to chicken B-cells is not known. Three monoclonal antibodies (T7, Q11 and Q13) against markers of immature B-cells of chicken were used to treat the bursal B-cells of young chickens (6 weeks old) before incubating them with IBDV. Immunoperoxidase staining of IBDV was used to assess the level of IBDV binding to B-cells without or after treatment with any of the three antibodies. The percentages of IBDV positive B-cells without and after treatment of B-cells with the T7, Q11 and Q13 antibodies were 93.42±0.47, 89.42±0.69, 91.75±0.35 and 91.58±0.57, respectively. The differences of mean values between the groups without antibody treatment and after antibody treatment were very significant in case of Q11 and Q13 antibodies (p = 0.001) and extremely significant (p<0.0001) in case of T7 antibody. ANOVA revealed an extremely significant (F = 9.624, p<0.0001) difference among the control and the three antibodies. A comparison of means between the various groups by Tukey's HSD test revealed significant differences between the control group and the three antibodies. The results suggest that the antibody T7 may be recognizing some epitope which may possibly be the target or located on or adjacent to the site of IBDV binding on B-cells.
Infectious Bursal Disease (IBD) is an acute contagious disease of young chickens with high morbidity and low mortality causing heavy losses to poultry industry worldwide. The disease is caused by IBD virus (IBDV), an Avibirnavirus which infects immature bursal B-cells of young chicken. However, the target molecule of B cells for binding of IBDV is not known. This review attempts to discuss the various possibilities and the current research in this direction.
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