Elenice Moreira Lemos

Universidade Federal do Espírito Santo

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Reynaldo Dietze

Universidade Federal do Espírito Santo

Fausto Edmundo Lima Pereira

Universidade Vila Velha

Sílvio Fernando Guimarães de Carvalho

Universidade Estadual de Montes Claros

Andréa Teixeira‐Carvalho

Fundação Oswaldo Cruz

Olindo Assis Martins‐Filho

Fundação Oswaldo Cruz

Geisa Baptista Barros

Universidade Federal do Espírito Santo

Suely G. Figueiredo

Universidade Federal do Espírito Santo

Olindo Assis Martins-Filho

Fundação Oswaldo Cruz

Alexandre Barbosa Reis

Universidade Federal de Ouro Preto

José Roberto Mineo

Universidade Federal de Uberlândia

Cooperative Institutions

Fundação Oswaldo Cruz

Universidade Federal do Espírito Santo

Universidade Federal de Minas Gerais

Universidade de São Paulo

Universidade Federal de Uberlândia

Universidade Federal de Ouro Preto

Universidade Estadual de Montes Claros

Universidade Federal do Rio de Janeiro

Universidade Federal de Goiás

Universidade Federal de São Paulo

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Proteomic analysis of the soluble proteomes of miltefosine-sensitive and -resistant Leishmania infantum chagasi isolates obtained from Brazilian patients with different treatment outcomes

Journal of Proteomics (2014)

Juliana B. T. Carnielli Hélida Monteiro de Andrade Simone F. Pires Alexander Chapeaurouge Jonás Perales

Miltefosine

Proteome

10.1016/j.jprot.2014.05.010

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Citations (34)

Anti-Toxocara antibodies detected in children attending elementary school in Vitoria, State of Espírito Santo, Brazil: prevalence and associated factors

Revista da Sociedade Brasileira de Medicina Tropical (2011)

Roberta Paranhos Fragoso Mariza Buriche Macedo Monteiro Elenice Moreira Lemos Fausto Edmundo Lima Pereira

INTRODUCTION: The aim of this study was to evaluate the frequency of anti-Toxocara antibodies in serum from 7-year-old children attending elementary school in Vitória-ES, Brazil and to correlate these antibodies with socio-demographic factors, the presence of intestinal helminths, blood eosinophil numbers, past history of allergy or asthma, and clinical manifestations of helminth infections. METHODS: The detection of anti-Toxocara antibodies was performed using an ELISA (Cellabs Pty Ltd)on serum from 391 children who had already been examined by fecal examination and blood cell counts. Data from clinical and physical examinations were obtained for all children. RESULTS: The prevalence of anti-Toxocara antibodies was 51.6%, with no gender differences. No significant differences were observed between positive serology and the presence or absence of intestinal worms (60.3 and 51.7%, respectively; p = 0.286). The only variables significantly related to positive serology were onycophagy and the use of unfiltered water. Although eosinophilia (blood eosinophil count higher than 600/mm³) was significantly related to the presence of a positive ELISA result, this significance disappeared when we considered only children without worms or without a past history of allergy or asthma. No clinical symptoms related to Toxocara infection were observed. CONCLUSIONS: There is a high prevalence of anti-Toxocara antibodies in children attending elementary schools in Vitória, which may be partially related to cross-reactivity with intestinal helminths or to a high frequency of infection with a small number of Toxocara eggs.

10.1590/s0037-86822011000400012

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Leishmania (Viannia) braziliensis: Immunoblotting analysis for the detection of IgG subclasses in the diagnosis of symptomatic and asymptomatic dogs

Veterinary Parasitology (2010)

Marcos Santos Zanini Kelvinson Fernandes Viana Alexandre Barbosa Reis Diefrey Ribeiro Campos Jamili Maria Suhet Mussi

During a seroepidemiological survey 2004-2006 from areas in Brazil endemic for American cutaneous leishmaniasis (ACL), serum samples from 10 dogs with ulcerated cutaneous lesions (S-ACL) and 52 asymptomatic dogs (AS-ACL) of unknown age and breed living in areas endemic for ACL were monitored for 1 year for ulcerated cutaneous lesions and immunoblotting using peroxidase-conjugated secondary anti-IgG, anti-IgG1 and anti-IgG2 dog antibodies. We reported that antibodies against Leishmania (Viannia) braziliensis in the sera of 22/52 dogs with asymptomatic disease showed intense reactivity to peptides larger than 66 kDa. We believe that dogs harboring subclinical amastigotes show an immunoblotting profile similar to that of symptomatic animals because a dog with self-healing presented antigens greater than 66 kDa. Such patterns can be exploited for diagnostic and epidemiological research for leishmaniasis.

10.1016/j.vetpar.2010.06.002

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Citations (4)

Upgrading the flow-cytometric analysis of anti-Leishmania immunoglobulins for the diagnosis of American tegumentary leishmaniasis

Journal of Immunological Methods (2008)

Jauber Fornaciari Pissinate Izabelle Teixeira Gomes Vanessa Peruhype-Magalhães Reynaldo Dietze Olindo Assis Martins‐Filho

10.1016/j.jim.2008.04.018

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Citations (20)

Laboratorial algorithm for serological diagnosis of visceral leishmaniasis using rK39-ICT, DAT-LPC and FC-Simplex IgG1

Journal of Immunological Methods (2020)

Isabela Natália Pascoal Campos do Vale Juliana Wilke Saliba Giuliana Schmidt França Fonseca Vanessa Peruhype-Magalhães Fernanda Fortes de Araújo

Simplex

10.1016/j.jim.2020.112765

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Citations (4)

Comparative proteomic analysis of miltefosine-sensitive and miltefosine-resistant leishmania chagasi clinical isolates

Proteómica: revista de la Sociedad Española de Proteómica (2013)

Juliana B. T. Carnielli Simone F. Pires Hélida Monteiro de Andrade Reynaldo Dietze Suely G. Figueiredo

Miltefosine

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Flow cytometry-based algorithm to analyze the anti-fixed Toxoplasma gondii tachyzoites IgM and IgG reactivity and diagnose human acute toxoplasmosis

Journal of Immunological Methods (2012)

Priscila Pinto e Silva-dos-Santos Geisa Baptista Barros José Roberto Mineo Deise Aparecida de Oliveira Silva Mauro Hygino Weinert Menegaz

Toxoplasmosis

Avidity

10.1016/j.jim.2012.02.001

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Citations (10)

Serological reactivity of different antigenic preparations of Leishmania (Leishmania) amazonensis and the Leishmania braziliensis complex

Revista da Sociedade Brasileira de Medicina Tropical (2008)

Adriano Gomes-Silva Maria Aparecida de Souza Sandra Regina Afonso-Cardoso Lívia Resende Andrade Reynaldo Dietze

Total antigen from Leishmania (Leishmania) amazonensis and isolates from the Leishmania braziliensis complex, along with their respective antigenic fractions obtained by affinity chromatography on concanavalin-A-Sepharose and jacalin-agarose columns evaluated using immunoenzymatic ELISA assay. For this, serum samples from 229 patients were used, grouped as American tegmental leishmaniasis (nº=58), visceral leishmaniasis (nº=28), Chagas disease (nº=49), malaria (nº=32), tuberculosis (nº=13) and healthy volunteers (nº=49). Samples from American tegmentary leishmaniasis showed higher reactivity with antigens isolated from the Leishmania braziliensis complex than with antigens from Leishmania amazonensis (p<0.001). ELISA assays showed a sensitivity range from 60% to 95% with antigens isolated from the Leishmania braziliensis complex. There was marked nonspecific reactivity among serum samples with the use of antigenic fractions binding with concanavalin-A and jacalin from both Leishmania complexes, in comparison with other antigens (p<0.001). The results presented in this study suggest that the use of homologous antigens increases the efficiency of anti-Leishmania immunoglobulin detection, which may be very valuable for diagnostic purposes.

Leishmania mexicana

Leishmania braziliensis

Cross-reactivity

Kinetoplastida

10.1590/s0037-86822008000200001

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Citations (28)

First description of autochthonous canine visceral leishmaniasis in the metropolitan region of Vitória, State of Espírito Santo, Brazil

Revista da Sociedade Brasileira de Medicina Tropical (2012)

Marco André Loureiro Tonini Elenice Moreira Lemos Alexandre Barbosa Reis Wendel Coura Vital Edelberto Santos Dias

We investigated autochthonous canine visceral leishmaniasis (CVL) in the metropolitan region of Vitória (MRV), an area in which a human case was previously reported.Serological, parasitological, and molecular tests were performed in 201 dogs.Twenty-six (13%) and 12 (6%) dogs were identified as positive using in-house enzyme-linked immunosorbent assay (ELISA) and rK39 tests, respectively. Two dogs had a positive culture for Leishmania chagasi, and 4 were polymerase chain reaction (PCR)-positive for Leishmania spp. One positive dog belonged to the aforementioned patient.Although the responsible vector was not found, our results provide evidence of autochthonous CVL in the MRV, a non-endemic area for VL.

10.1590/s0037-86822012000600019

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Citations (10)

Avaliação do desempenho da pesquisa de anticorpos anti-Toxoplasma por citometria de fluxo no diagnóstico da toxoplasmose aguda humana

Elenice Moreira Lemos Deise Aparecida de Oliveira Silva Fausto Edmundo Lima Pereira

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