Peter F. Lemkin

University of the Potomac

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Lewis E. Lipkin

National Cancer Institute

Gregory C. Thornwall

Science Applications International Corporation (United States)

George Carman

Rutgers, The State University of New Jersey

Djamel Medjahed

National Cancer Institute

Jai Evans

Lockheed Martin (United States)

David J. Munroe

Columbia University

Bruce A. Shapiro

National Cancer Institute

Gary W. Smythers

National Cancer Institute

MORTON SCHULTZ

National Cancer Institute

Eric P. Lester

University of Chicago

Cooperative Institutions

National Institutes of Health

Frederick National Laboratory for Cancer Research

National Cancer Institute

University of Manchester

Institute of Electrical and Electronics Engineers

Johns Hopkins University

University of Liverpool

Wellcome Trust

Science Applications International Corporation (United States)

University of Chicago

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The protein disease database

Electrophoresis (1995)

Peter F. Lemkin G A Orr M. Goldstein Joseph Creed Edward Whitley

Creed

Section (typography)

10.1002/elps.11501601194

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Citations (10)

MAExplorer – microarray exploratory data analysis

Nature Genetics (1999)

Peter F. Lemkin Greggory Thornwall Ulli Wagner Lothar Henninghausen

Microarray databases

Exploratory analysis

Exploratory data analysis

10.1038/14346

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Citations (1)

Human leukemias: A preliminary 2-D electrophoretic analysis

Electrophoresis (1982)

Eric P. Lester Peter F. Lemkin Joyce F. Lowery Lewis E. Lipkin

Abstract We have investigated the qualitative and quantitative differences in the two‐dimensional (2‐D) gel fluorographic patterns of biosynthetically pulse‐labeled cellular proteins from the malignant cells of patients with various leukemias (acute myelogenous, acute lymphoblastic, chronic lymphocytic, and hairy cell). Significant similarities and differences between the major types of leukemias were found. An initial small data base was constructed using our GELLAB computer analysis system for 2‐D gels in order to define methods for data base definition, normalization, and visual verification of results. Search strategies to find significant spot differences, both qualitative and quantitative, between currently accepted classes of leukemias were explored. Potential methods of expressing in a summary statistic the overall similarities and differences between gels were also investigated. A series of proteins whose relative synthetic rates may serve as potential markers of states of differentiation in human leukemias were identified in this initial data base and are reported here.

Normalization

Chronic myelogenous leukemia

Base (topology)

Statistic

Quantitative Analysis

Acute lymphocytic leukemia

10.1002/elps.1150030612

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Citations (16)

A World Wide Web (WWW) server database engine for an organelle database, MitoDat

Electrophoresis (1996)

Peter F. Lemkin M. Chipperfield Carl R. Merril Steven J. Zullo

We describe a simple database search engine "dbEngine" which may be used to quickly create a searchable database on a World Wide Web (WWW) server. Data may be prepared from spreadsheet programs (such as Excel, etc.) or from tables exported from relationship database systems. This Common Gateway Interface (CGI-BIN) program is used with a WWW server such as available commercially, or from National Center for Supercomputer Algorithms (NCSA) or CERN. Its capabilities include: (i) searching records by combinations of terms connected with ANDs or ORs; (ii) returning search results as hypertext links to other WWW database servers; (iii) mapping lists of literature reference identifiers to the full references; (iv) creating bidirectional hypertext links between pictures and the database. DbEngine has been used to support the MitoDat database (Mendelian and non-Mendelian inheritance associated with the Mitochondrion) on the WWW.

Hypertext

Database server

Interface (matter)

Gateway (web page)

Hyperlink

10.1002/elps.1150170327

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Citations (19)

Xconf: A Network-Based Image Conferencing System

Computers and Biomedical Research (1993)

Peter F. Lemkin

Videoconferencing

Teleconference

Collaborative software

Computer-supported cooperative work

10.1006/cbmr.1993.1001

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Citations (9)

An approach to region splitting

Computer Graphics and Image Processing (1979)

Peter F. Lemkin

10.1016/0146-664x(79)90007-8

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Citations (3)

HIGH RESOLUTION SHADING CORRECTION

Journal of Histochemistry & Cytochemistry (1974)

MORTON SCHULTZ Lewis E. Lipkin MARTA J. WADE Peter F. Lemkin George Carman

Quantitative cytology requires accurate representation of a specimen's optical densities. As the requirements for measurement precision increase, instrument-induced errors become increasingly more difficult to reduce to the point at which their effect on experimental data is insignificant compared to the measured parameters. Shading induces a significant amount of amplitude ambiguity to data obtained from a scanning system. A method of shading correction on single pixels is introduced as a new way to reduce some errors that currently plague scanning systems.

Shading

Photometric Stereo

10.1177/22.7.751

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Citations (17)

Proteome Knowledge Bases in the Context of Cancer

CRC Press eBooks (2005)

Djamel Medjahed Peter F. Lemkin

Proteome

Human proteome project

10.1201/9781420027624.ch6

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Citations (0)

Two‐dimensional protein electrophoresis and multiple hypothesis testing to detect potential serum protein biomarkers in children with fetal alcohol syndrome

Electrophoresis (1995)

Mary K. Robinson James E. Myrick L. Omar Henderson Claire D. Coles Mary K. Powell

Abstract Fetal alcohol syndrome (FAS) surveillance and intervention efforts are hampered by the lack of a specific biochemical test for diagnosis of the syndrome. Based on the hypothesis that abnormalities in growth and development (key features of FAS) involve altered protein metabolism, we analyzed serum proteins by two‐dimensional gel electrophoresis and image analysis to search for potential protein biomarkers of FAS. Serum samples from 12 participants in whom FAS had been diagnosed and 8 sex‐ and age‐matched participants whose mothers did not consume alcohol were analyzed in duplicate to determine whether the integrated intensities of matched proteins are significantly altered in children with FAS. Multiple hypothesis testing on 34 of the gels consisting of more than 1700 spots per gel revealed 21 proteins that we classified as potential protein biomarkers of FAS on the basis of significant t ‐test differences at p < 0.02. We classified 8 of the proteins as candidate biomarkers on the basis of significant concentration differences between case and control subjects at p < 0.01. One of the proteins is clearly an isoform of retinol binding protein; two appear in the area of the gel where alcohol dehydrogenase is expected to appear; one appears to be an isoform of alpha‐1‐antitrypsin; three appear to be isoforms of the beta‐chain of haptoglobin; three may be forms of immunoglobulin light chains; and several others have not been associated with known proteins. No single protein differentiated all case subjects from control subjects, but stepwise canonical discriminant analyses revealed four groups of spots that distinguished between FAS case and control subjects with no misclassifications. Because of the small number of samples analyzed, we have not positively identified the proteins. It will be necessary to confirm our observations using additional samples before identifying each protein.

Fetal Alcohol Syndrome

Prenatal Alcohol Exposure

Fetal alcohol

10.1002/elps.11501601195

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Citations (29)

Representations of Protein Patterns From Two-Dimensional Gel Electrophoresis Databases

WORLD SCIENTIFIC eBooks (1995)

Peter F. Lemkin

10.1142/9789812832054_0006

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Citations (0)