Pierre Sanchez

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

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Pierre‐André Cazenave

Université de Lille

Jean Martínez

Durango Institute of Technology

Anne‐Marie Drapier

Institut Pasteur

Gilles Subra

Centre National de la Recherche Scientifique

Christine Enjalbal

Institut des Biomolécules Max Mousseron

Marie‐Odile Blanc

Marine Biodiversity Exploitation and Conservation

Jean‐Louis Aubagnac

Université de Montpellier

Alfredo Blanco Quirós

Universidad de Valladolid

Frédéric Clota

Marine Biodiversity Exploitation and Conservation

Alain Vergnet

Ifremer

Cooperative Institutions

Centre National de la Recherche Scientifique

Université de Montpellier

Institut des Biomolécules Max Mousseron

Inserm

École Nationale Supérieure de Chimie de Montpellier

Institut Pasteur

Sorbonne Université

Université Paris Cité

University of Milano-Bicocca

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

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Available λ B cell repertoire in the mouse: Evidence of positive selection by environmental factors

European Journal of Immunology (1993)

Bertrand Nadel Anne‐Marie Drapier Pierre‐André Cazenave Pierre Sanchez

Abstract We have recently shown that, from two BALB/c mice treated with rabbit anti‐Cλ2/Cλ3 antibodies coupled to lipopolysaccharide, variable heavy chain (V H ) family repertoires associated with λ2 or λ3 light chains can differ from one λ. subtype to another and from one individual mouse to another. Indeed, 4 out of 6 λ2 (VxJ2) hybridomas from one mouse preferentially expressed the V H 10 family while 3 out of 8 λ2 (V2J2) and 5 out of 8 λ2 (VxJ2) hybridomas from a second mouse preferentially expressed the S107 and VGAM3.8 V H families, respectively. In this report, we describe the structural basis of such preferential pairings by sequence analysis of the 12 λ2 hybridomas. The sequence comparison of their V H regions show that each preferential association of a VH family to one Vλ region is restricted to the use of a single member or very closely related members inside a V H family and that a great variability of CDR3 of heavy chain is observed. We, therefore, suggest that environmental factors can modify the available XλB cell repertoire through a positive selection of particular VH/Vλ pairings. Moreover, our data support that this selection does not require clonal expansion and punctual somatic mutation.

Positive selection

Sequence (biology)

Heavy chain

10.1002/eji.1830230236

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Citations (10)

Surface Enhanced Raman Spectroscopy (SERS) as an emerging tool to monitor protease activity : Synthesis of peptide-Adenine conjugates as SERS probes

HAL (Le Centre pour la Communication Scientifique Directe) (2018)

Nicolas Masurier Feryel Soualmia Pierre Sanchez Valérie Lefort Mia Roué

Surface-Enhanced Raman Spectroscopy

Conjugate

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Supported oligomethionine sulfoxide and Ellman’s reagent for cysteine bridges formation

Amino Acids (2012)

Luisa Ronga Pascal Verdié Pierre Sanchez Christine Enjabal Amélie Maurras

Sulfoxide

10.1007/s00726-012-1397-5

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Citations (7)

Dictionnaire des graveurs, illustrateurs et affichistes français et étrangers (1673-1950)

Gaïte Dugnat Pierre Sanchez Christian Galantaris

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V_λ‐J_λ rearrangements are restricted within a V‐J‐C recombination unit in the mouse

European Journal of Immunology (1991)

Pierre Sanchez Bertrand Nadel Pierre‐André Cazenave

Abstract The murine λ gene locus is organized as follows: V λ 2‐V λ x‐J λ 2C λ 2‐ΨJ λ 4C λ 4‐V λ 1‐J λ 3C λ 3‐J λ 1C λ 1 where all segments have the same transcriptional orientation. The combinatorial process of gene recombination should allow the generation of eight distinct immunoglobulin light chains. We have therefore investigated the probability of obtaining such chains among the mature λ B cell repertoire. We analyze serum λ immunoglobulins and λ B cell clones induced by treatment with rabbit anti‐Δ antibodies coupled to LPS. Confirming previous data obtained by others, our results indicate that the rearrangements of Δ segments take place within each V λ ‐J λ ‐C λ cluster, thereby defining a unit of recombination. Our results also provide no evidence for the use of undescribed segments as has been recently suggested by the finding of the V λ x segment.

Immunoglobulin heavy chain

Gene rearrangement

V(D)J recombination

Immunoglobulin gene

10.1002/eji.1830210408

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Citations (50)

Various V‐J rearrangement efficiencies shape the mouse λ B cell repertoire

European Journal of Immunology (1995)

Pierre Boudinot Dominique Rueff‐Juy Anne‐Marie Drapier Pierre‐André Cazenave Pierre Sanchez

The diversity of the B cell repertoire of C kappa knockout mice is limited by the expression of four lambda light chain types. Among the spleen B cells, lambda 1 is expressed by the majority (58%) of cells, and lambda 3 by the minority (8%), while lambda 2 (V2) and lambda 2 (Vx) are expressed in intermediate quantities (18% and 16%, respectively). To assess the influence of mechanistic pressures on the lambda subtype distribution, the proportions of the different lambda rearrangements were determined in various B cell subpopulations divided on the basis of the lambda subtype expressed, and the V lambda J lambda junction sequences were studied at different steps of B cell differentiation (pre-B, immature and mature B cells). The data show that (1) the ratio of productive/non-productive VJ junctions is determined by the nature of the lambda segments that are rearranged as can be observed in the pre-B cells, (2) V1-J1 non-productive rearrangements are often found in the lambda 1-negative B cells in the periphery, and (3) V1J3 junctions are often non-productive regardless of the nature of the cells analyzed. Our results, therefore, suggest that a strong probability of initiating a V1-J1 rearrangement and a weak probability of giving a productive V1J3 junction are responsible for the lambda 1 dominance and the lambda 3 under-expression, respectively. The intermediate proportion of lambda 2(V2) subtype is most likely due to a probability of obtaining a productive joint that is better than that for V1J3 and a probability of initiating a rearrangement that is lower than that for V1J1. However, the lambda 2(Vx) cell proportion cannot be determined only by these parameters.

Gene rearrangement

Dominance (genetics)

10.1002/eji.1830250914

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Citations (11)

Synthesis of Peptide–Adenine Conjugates as a New Tool for Monitoring Protease Activity

European Journal of Organic Chemistry (2018)

Nicolas Masurier Feryel Soualmia Pierre Sanchez Valérie Lefort Mia Roué

We took advantage of the powerful adenine SERS (Surface Enhanced Raman Spectroscopy) probe to design peptide–adenine conjugates as candidates for use as serine protease substrates. Whereas the direct introduction of the peptide sequence on the adenine exocyclic N6 amine gave an imidazopurinone derivative, the introduction of an aminoethyl linker between the adenine group and the peptide chain led to the expected candidate probes. These potential substrates were then evaluated for monitoring the hydrolytic activity of trypsin, used as a model protease, by HPLC and by SERS. We demonstrated that the Boc–VPR–adenine conjugate is a substrate of trypsin and constitutes a good starting point to design optimized substrates to monitor protease activity by SERS.

Linker

Conjugate

10.1002/ejoc.201801490

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Citations (2)

R-phycoerythrin-cyanine 5 tandem discerns CD72 polymorphism

Immunogenetics (2001)

Magali Doucet Neirouz Soussi Anne‐Marie Crain‐Denoyelle Marie‐Claude Gendron Pierre Sanchez

Cyanine

10.1007/s002510100331

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Citations (8)

Utilization of charge and mass labeling for the structural identification of heterocyclic quaternary salts by mass spectrometry

International Journal of Mass Spectrometry (2002)

Christine Enjalbal Pierre Sanchez Jean Martínez Jean‐Louis Aubagnac Dionísia Sanz

Characterization

Top-down proteomics

Ion-mobility spectrometry–mass spectrometry

Identification

10.1016/s1387-3806(02)00695-4

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Citations (5)

The λ B Cell Repertoire of K-Deficient Mice

International Reviews of Immunology (1996)

Pierre Sanchez Dominique Rueff‐Juy Pierre Boudinot Soulef Hachemi‐Rachedi Pierre‐André Cazenave

Analysis of the B cell repertoire is complicated by the huge diversity inherent in the germ line determined combinatory. Making use of knockout technology, K-deficient mice have been obtained. They constitute a shrewd model to follow the expression of an Ig minilocus, such as the λ one, in the normal condition compared with classical transgenic models. Indeed, in contrast to wild type mice, in which only 5% of λ B cells are produced, these mutant mice exclusively produce λ positive B cells. Although, the λ locus is well characterized and has a relatively simple organization, the mechanistic and selective pressures that govern its utilization are still poorly understood. The analysis of the λ B cell repertoire in K-deficient mice, should therefore bring more conclusive informations. Here we present the λ subtype distribution in the various cellular compartments of the K-deficient mice, and discuss the rules that can be responsible for this distribution. Our recent data indicate that the λ subtype proportions in the bone marrow and the spleen result, for the major part, from mechanistic processes (i.e., recombinase accessibility, production of V-J functional joint and H/L pairings) while the λ proportions found in the peritoneal cavity ensue from selective processes. Finally, the capacity to respond to various antigens is discussed from such a generated λ B cell repertoire.

10.3109/08830189609061758

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Citations (11)