José Paulo Gagliardi Leite

Fundação Oswaldo Cruz

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Marize Pereira Miagostovich

Fundação Oswaldo Cruz

Túlio Machado Fumian

Fundação Oswaldo Cruz

Alexandre Madi Fialho

Fundação Oswaldo Cruz

Filipe Aníbal Carvalho‐Costa

Fundação Oswaldo Cruz

Rosane Maria Santos de Assis

Fundação Oswaldo Cruz

Marcelle Figueira Marques da Silva

Universidade Federal de Goiás

Eduardo de Mello Volotão

Fundação Oswaldo Cruz

Mariela Martínez Gómez

World Health Organization Regional Office for the Americas

Márcia Terezinha Baroni de Moraes

Fundação Oswaldo Cruz

Joana D’Arc Pereira Mascarenhas

Ministério da Saúde

Cooperative Institutions

Fundação Oswaldo Cruz

219

Ministério da Saúde

Universidade Federal Fluminense

Universidade Federal do Rio de Janeiro

Centers for Disease Control and Prevention

Instituto Evandro Chagas

Christian Medical College & Hospital

Faculdades Oswaldo Cruz

University of Virginia

Universidade Federal do Ceará

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Analysis of human P[4]G2 rotavirus strains isolated in Brazil reveals codon usage bias and strong compositional constraints

Infection Genetics and Evolution (2011)

Mariela Martínez Gómez Luis Fernando López Tort Eduardo de Mello Volotão Ricardo Recarey Gonzalo Moratorio

CpG site

Genetic Code

10.1016/j.meegid.2011.01.006

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Citations (15)

Epidemiology of rotavirus A diarrhea in Chókwè, Southern Mozambique, from February to September, 2011

Journal of Medical Virology (2016)

Jerónimo S. Langa R.C.A. Thompson Paulo Arnaldo Hugo Reis Resque Tatiana L. Rose

Acute diarrhea disease caused by Rotaviruses A (RVA) is still the leading cause of morbidity and mortality in children ≤5 years old in developing countries. An exploratory cross‐sectional study was conducted between February and September, 2011 to determine the proportion of acute diarrhea caused by RVA. A total of 254 stool specimens were collected from children ≤5 years old with acute diarrhea, including outpatients (222 children) and inpatients (32 children), in three local health centers in Chókwè District, Gaza Province, South of Mozambique. RVA antigens were detected using enzyme immunoassay (EIA); the RVA G (VP7) and P (VP4) genotypes were determined by RT‐PCR or analysis sequencing. Sixty (24%) out of 254 fecal specimens were positive for RVA by EIA; being 58 (97%) from children ≤2 years of age. RVA prevalence peaks in June and July (coldest and drier months) and the G[P] binary combination observed were G12P[8] (57%); G1P[8] (9%); G12P[6] (6%); and 2% for each of the following genotypes: G1P[6], G2P[6] G4P[6], and G9P[8]. Non‐Typeable (NT) G and/or P genotypes were observed as follows: G12P [NT] (6%); G1P [NT], G3P[NT] and GNTP[NT] (4%). Considering the different GP combinations, G12 represented 67% of the genotypes. This is the first data showing the diversity of RVA genotypes in Mozambique highlighting the epidemiological importance of these viruses in acute diarrhea cases in children ≤2 years old. In addition, these findings will provide a baseline data before the introduction of the RVA monovalent (Rotarix ® ) vaccine in the National Immunization Program in September 2015. J. Med. Virol. 88:1751–1758, 2016 . © 2016 Wiley Periodicals, Inc.

Acute gastroenteritis

Group A

10.1002/jmv.24531

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Citations (22)

Detection of Rocahepevirus ratti in Bivalve Mollusks from São Luís Island, Maranhão, Brazil: A Potential Transmission Route of an Emerging Zoonotic Pathogen?

Food and Environmental Virology (2025)

Andreza Soriano Figueiredo Ida Negreiros Aldaleia do Nascimento e Silva Caroline Roberta Soares Salgado N. L. dos SANTOS

Hepatitis E Virus

10.1007/s12560-024-09624-0

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Citations (0)

Rotavirus genotypes in children under five years hospitalized with diarrhea in low and middle-income countries: Results from the WHO-coordinated Global Rotavirus Surveillance Network

PLOS Global Public Health (2023)

Sébastien Antoni Tomoka Nakamura Adam L. Cohen Jason M. Mwenda Goitom Weldegebriel

Rotavirus is the most common pathogen causing pediatric diarrhea and an important cause of morbidity and mortality in low- and middle-income countries. Previous evidence suggests that the introduction of rotavirus vaccines in national immunization schedules resulted in dramatic declines in disease burden but may also be changing the rotavirus genetic landscape and driving the emergence of new genotypes. We report genotype data of more than 16,000 rotavirus isolates from 40 countries participating in the Global Rotavirus Surveillance Network. Data from a convenience sample of children under five years of age hospitalized with acute watery diarrhea who tested positive for rotavirus were included. Country results were weighted by their estimated rotavirus disease burden to estimate regional genotype distributions. Globally, the most frequent genotypes identified after weighting were G1P[8] (31%), G1P[6] (8%) and G3P[8] (8%). Genotypes varied across WHO Regions and between countries that had and had not introduced rotavirus vaccine. G1P[8] was less frequent among African (36 vs 20%) and European (33 vs 8%) countries that had introduced rotavirus vaccines as compared to countries that had not introduced. Our results describe differences in the distribution of the most common rotavirus genotypes in children with diarrhea in low- and middle-income countries. G1P[8] was less frequent in countries that had introduced the rotavirus vaccine while different strains are emerging or re-emerging in different regions.

Rotavirus vaccine

Acute diarrhea

10.1371/journal.pgph.0001358

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Citations (9)

Development of a rapid and sensitive latex agglutination-based method for detection of group A rotavirus

Journal of Virological Methods (2008)

A.C. Goes Márcia Terezinha Baroni de Moraes Waldemir de Castro Silveira Irene Trigueiros Araújo Jean-Claude de H’alluin

Latex fixation test

Agglutination (biology)

10.1016/j.jviromet.2007.11.013

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Citations (10)

Detection and molecular characterization of caliciviruses (vesivirus and norovirus) in an outbreak of acute diarrhea in kittens from Brazil

The Veterinary Journal (2015)

Tatiana Xavier de Castro Rita de Cássia Nasser Cubel Garcia Túlio Machado Fumian Erika Moutinho Costa Renata Mello

Astrovirus

Feline calicivirus

Caliciviridae

Canine parvovirus

10.1016/j.tvjl.2015.06.014

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Citations (14)

Genetic diversity of porcine enteric caliciviruses in pigs raised in Rio de Janeiro State, Brazil

Archives of Virology (2010)

Juliana Bragazzi Cunha Marcos César Lima de Mendonça Marize Pereira Miagostovich José Paulo Gagliardi Leite

Sapovirus

Caliciviridae

Astrovirus

10.1007/s00705-010-0695-z

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Citations (33)

Protection From Natural Immunity Against Enteric Infections and Etiology-Specific Diarrhea in a Longitudinal Birth Cohort

The Journal of Infectious Diseases (2020)

Elizabeth T. Rogawski McQuade Jie Liu Gagandeep Kang Margaret Kosek Aldo Â. M. Lima

Abstract Background The degree of protection conferred by natural immunity is unknown for many enteropathogens, but it is important to support the development of enteric vaccines. Methods We used the Andersen-Gill extension of the Cox model to estimate the effects of previous infections on the incidence of subsequent subclinical infections and diarrhea in children under 2 using quantitative molecular diagnostics in the MAL-ED cohort. We used cross-pathogen negative control associations to correct bias due to confounding by unmeasured heterogeneity of exposure and susceptibility. Results Prior rotavirus infection was associated with a 50% lower hazard (calibrated hazard ratio [cHR], 0.50; 95% confidence interval [CI], 0.41–0.62) of subsequent rotavirus diarrhea. Strong protection was evident against Cryptosporidium diarrhea (cHR, 0.32; 95% CI, 0.20–0.51). There was also protection due to prior infections for norovirus GII (cHR against diarrhea, 0.67; 95% CI, 0.49–0.91), astrovirus (cHR, 0.62; 95% CI, 0.48–0.81), and Shigella (cHR, 0.79; 95% CI, 0.65–0.95). Minimal protection was observed for other bacteria, adenovirus 40/41, and sapovirus. Conclusions Natural immunity was generally stronger for the enteric viruses than bacteria, potentially due to less antigenic diversity. Vaccines against major causes of diarrhea may be feasible but likely need to be more immunogenic than natural infection.

Sapovirus

Astrovirus

Seroconversion

Subclinical infection

10.1093/infdis/jiaa031

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Citations (35)

Distinct evolutionary origins of G12P[8] and G12P[9] group A rotavirus strains circulating in Brazil

Infection Genetics and Evolution (2014)

Mariela Martínez Gómez Hugo Reis Resque Eduardo de Mello Volotão Tatiana Lundgren Rose Marcelle Figueira Marques da Silva

G12 group A rotavirus (RVA) are currently recognized as a globally emerging genotype and have been described in combination with several P-types. In Brazil, G12 RVA strains have been described in the Southern (2003) and Northern (2008-2010) regions, in combination with the P[9] and P[6] genotype, respectively. To date, few complete genomes of G12 RVA strains have been described (none from Brazilian strains), considering G12P[9] genotype just one strain, RVA/Human-tc/THA/T152/1998/G12P[9], has their 11 gene segments characterized. This study aims to determine the genomic constellation of G12P[9] and G12P[8] RVA strains detected in Brazil between 2006 and 2011. Therefore, the eleven gene segments of five Brazilian G12 RVA strains were amplified and sequenced, and the genotype of each gene segment was assigned using phylogenetic analysis. Complete genome analyses of G12 RVA strain circulating between 2006 and 2011 in Brazil revealed a conserved Wa-like genomic constellation for three G12P[8] RVA strains; whereas the two G12P[9] strains possessed distinct reassorted AU-1-like genomic constellations, closely related to the reference strain RVA/Human-tc/THA/T152/1998/G12P[9] in most genes. The results obtained in the current study suggest that G12P[9] (AU-1-like) and G12P[8] (Wa-like) strains detected in different regions of Brazil do not share a common origin. Moreover, while Brazilian G12P[8] RVA strains showed a complete Wa-like human constellation, both G12P[9] strains possessed an NSP1 gene of bovine origin (NSP1), and RVA/Human-wt/BRA/PE18974/2010/G12P[9] also possessed a VP3 gene of canine/feline origin.

Strain (injury)

10.1016/j.meegid.2014.04.007

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Citations (31)

Detection and molecular characterization of group A rotavirus from hospitalized children in Rio de Janeiro, Brazil, 2004

Memórias do Instituto Oswaldo Cruz (2006)

Filipe Aníbal Carvalho‐Costa Rosane Maria Santos de Assis Alexandre Madi Fialho Márcio Neves Bóia Daniele Pires Dias Alves

Rotavirus is a major cause of infantile acute diarrhea, causing about 440,000 deaths per year, mainly in developing countries. The World Health Organization has been recommending the assessment of rotavirus burden and strain characterization as part of the strategies of immunization programs against this pathogen. In this context, a prospective study was made on a sample of 134 children with acute diarrhea and severe dehydration admitted to venous fluid therapy in two state hospitals in Rio de Janeiro, Brazil, from February to September 2004. Rotavirus where detected by polyacrylamide gel electrophoresis (PAGE) and by an enzyme-linked immunoassay to rotavirus and adenovirus (EIARA) in 48% of the children. Positive samples for group A rotavirus (n = 65) were analyzed by reverse transcription/heminested multiplex polymerase chain reaction to determine the frequency of G and [P] genotypes and, from these, 64 samples could be typed. The most frequent G genotype was G1 (58%) followed by G9 (40%). One mixed infection (G1/G9) was detected. The only [P] genotype identified was [8]. In order to estimate the rotavirus infection frequency in children who acquired diarrhea as hospital infection in those hospitals, we studied 24 patients, detecting the pathogen in 41% of them. This data suggest that genotype G9 is an important genotype in Rio de Janeiro, with implications to the future strategies of vaccination against rotavirus, reinforcing the need of continuous monitoring of circulating strains of the pathogen, in a surveillance context.

Acute diarrhea

Multiplex

Group A

10.1590/s0074-02762006000300012

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Citations (36)