Supplementary Figure 5 from EGFR Signals Downregulate Tumor Suppressors miR-143 and miR-145 in Western Diet–Promoted Murine Colon Cancer: Role of G<sub>1</sub> Regulators
Bleeding and thrombosis in critically ill infants and children is a vexing clinical problem. Despite the relatively low incidence of bleeding and thrombosis in the overall pediatric population relative to adults, these critically ill children face unique challenges to hemostasis due to extreme physiologic derangements, exposure of blood to foreign surfaces and membranes, and major vascular endothelial injury or disruption. Caring for pediatric patients on extracorporeal support, recovering from solid organ transplant or invasive surgery, and after major trauma is often complicated by major bleeding or clotting events. As our ability to care for the youngest and sickest of these children increases, the gaps in our understanding of the clinical implications of developmental hemostasis have become increasingly important. We review the current understanding of the development and function of the hemostatic system, including the complex and overlapping interactions of coagulation proteins, platelets, fibrinolysis, and immune mediators from the neonatal period through early childhood and to young adulthood. We then examine scenarios in which our ability to effectively measure and treat coagulation derangements in pediatric patients is limited. In these clinical situations, adult therapies are often extrapolated for use in children without taking age-related differences in pediatric hemostasis into account, leaving clinicians confused and impacting patient outcomes. We discuss the limitations of current coagulation testing in pediatric patients before turning to emerging ideas in the measurement and management of pediatric bleeding and thrombosis. Finally, we highlight opportunities for future research which take into account this developing balance of bleeding and thrombosis in our youngest patients.
Crude oil refining is a global insert responsible for the production of a wide array of petroleum based products crucial for various sectors including energy, transportation, and manufacturing. However, the artisanal form of crude oil refining, often characterized by its small scale and limited technological infrastructure, has emerged as a major environmental concern in several regions around the world. Illegal refining of crude oil has led to oil spillages which in turn lead to a disruption of the microbial population of the soil. Hence, the aim of this study was to isolate and characterize the heterotrophic and hydrocarbon utilizing bacteria and fungi from an artisanal crude oil refining site. A total of thirty six (36) soil samples were collected from three (3) artisanal crude oil refining sites at iwofe, ogbogoro and ibaa community all in Rivers State, Nigeria. The samples were collected using soil auger and subjected to standard microbiological procedures such as culturing, isolation and identification. The results revealed significantly elevated levels of heterotrophic and hydrocarbon utilizing bacteria and fungi in the soil samples, with notable variations (p≤0.05) across the different artisanal crude oil refining sites. Total Heterotrophic Bacteria (THB) counts ranged from 3.75x105CFU/g in Iwofe to 2.70x105CFU/g in Ibaa, Total heterotrophic Fungal (THF) counts ranged from 2.80x104CFU/g recorded in Ogbogoro to 1.45x104CFU/g recorded in Iwofe, Hydrocarbon Utilizing Bacteria (HUB) counts ranged from 2.25x103CFU/g in Ibaa to 1.30x103CFU/g in Iwofe and Hydrocarbon Utilizing Fungi (HUF) counts ranged from 2.50x102CFU/g recorded in Ogbogoro to 1.40x102CFU/g recorded in Ibaa community with significant differences (p≤0.05). Bacteria isolated and characterized from the soil include Enterobacter, Pseudomonas putida, Serratia and Bacillus amyloliquefaciens while fungi include Fusarium, Aspergillus, Penicillium and Mucor. The microorganisms identified in this study have been reported to play key roles in the utilization of crude oil. Pseudomonas and Bacillus were the most dominant bacterial isolates and their dominance could imply that the soil could support the growth of crops. Thus, while they utilize the hydrocarbons, they also serve as plant-growth promoting microorganisms. Although some of the microorganisms in the present study have been reported to promote the growth of plant, the dumping of crude oil products legal or illegal should be minimized to avoid the extinction of plant-promoting microorganisms.
Abstract There are more than 70 distinct sarcomas, and this diversity complicates the development of precision-based therapeutics for these cancers. Prospective comprehensive genomic profiling could overcome this challenge by providing insight into sarcomas’ molecular drivers. Through targeted panel sequencing of 7494 sarcomas representing 44 histologies, we identify highly recurrent and type-specific alterations that aid in diagnosis and treatment decisions. Sequencing could lead to refinement or reassignment of 10.5% of diagnoses. Nearly one-third of patients (31.7%) harbor potentially actionable alterations, including a significant proportion (2.6%) with kinase gene rearrangements; 3.9% have a tumor mutational burden ≥10 mut/Mb. We describe low frequencies of microsatellite instability (<0.3%) and a high degree of genome-wide loss of heterozygosity (15%) across sarcomas, which are not readily explained by homologous recombination deficiency (observed in 2.5% of cases). In a clinically annotated subset of 118 patients, we validate actionable genetic events as therapeutic targets. Collectively, our findings reveal the genetic landscape of human sarcomas, which may inform future development of therapeutics and improve clinical outcomes for patients with these rare cancers.
Aims & Objectives: A proportion of children with life-threatening conditions (LTC) are potentially eligible to be organ and/or tissue donors. While research has focused on donation rates and best practice, relatively little is known about parents' experiences of requests (or not) for donation. Increased understanding of parents' experiences will hopefully enable improved decision-making and support. Methods Bereaved parents and parents of a child with LTC were interviewed to investigate experiences of requests for organ and tissue donation. Recruited via 2 NICU, 2 PICU, 1 Cardiac ICU and 1 children's hospice. Parents asked about donation, specifically whether they were asked, and their experiences related to the request and the donation (if applicable). Thematic analysis carried out to generate overarching themes. Results 24 parents of 20 children interviewed: 21 bereaved, 3 child with LTC. 7 parents/children were asked about donation (13 not) - 4 agreed, 2 donated. Five overarching themes identified: 1) difficulty of timing of request, 2) importance of altruism and child's legacy around decision-making, 3) request, or lack of request, as judgement/indication of child's value, 4) emotional cost to staff and 5) negative aspects, e.g. paperwork, brain-death tests. Conclusions Parents are aware of the cost to staff in asking, and that there is an emotional cost for parents when not asked, as they interpret this as judgement about their child's value. No parent reported insensitive or inappropriate approaches by health professionals. A request should be made, if eligibility allows, as parents can derive comfort from the thought that their child might be suitable for donation.
Supplementary Figure 3 from EGFR Signals Downregulate Tumor Suppressors miR-143 and miR-145 in Western Diet–Promoted Murine Colon Cancer: Role of G<sub>1</sub> Regulators
Objective A proportion of children die, making them potentially eligible to be organ/tissue donors. Not all are approached for donation, and experiences of those parents are not well understood. The objective was to investigate to what extent organ and tissue donation (OTD) is discussed as part of end-of-life care and to explore parents’ and healthcare professionals’ (HCPs) experiences. Design A retrospective qualitative study. Setting Multicentre study with participants recruited through two neonatal intensive care units (ICUs), two paediatric ICUs, a cardiac ICU and a children’s hospice. Patients Bereaved parents, parents of a child with a long-term condition (LTC) and HCPs. Interventions None. Main outcomes and measures Parents’ and HCPs’ views and experiences of discussions about OTD. Results 24 parents of 20 children were interviewed: 21 bereaved parents and 3 parents of a child with a LTC. Seven parents were asked about donation (13 not asked), four agreed and two donated. 41 HCPs were interviewed. Themes: complexity of donation process, OTD as a coping strategy, the importance of asking, difficulty of raising the topic,\ and parents’ assumptions about health of organs (when donation is not discussed). Conclusions The findings add new knowledge about parents’ assumptions about the value of their child’s organs when discussions about OTD are not raised, and that HCPs do not routinely ask, are sometimes hesitant to ask in fear of damaging relationships, and the reality of the complexity of the donation process. Given the current levels of awareness around OTD, the topic should be raised.
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