Zhilei Li

Southern University of Science and Technology

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Wensheng Xiang

Chinese Academy of Agricultural Sciences

Xiangjing Wang

Northeast Agricultural University

Chongxi Liu

Northeast Agricultural University

Junwei Zhao

Northeast Agricultural University

Yuxin Cui

First Affiliated Hospital of Xi'an Jiaotong University

Wen G. Jiang

Cardiff University

Jane Lane

Cardiff University

Yanpeng Li

Southern Medical University

Kutty Selva Nandakumar

Southern Medical University

Shouyi Wu

Lanzhou University

Cooperative Institutions

Capital Medical University

Northeast Agricultural University

Chinese Academy of Sciences

Southern Medical University

Lanzhou University

Beijing Tian Tan Hospital

Shanghai Jiao Tong University

Chinese Academy of Agricultural Sciences

First Affiliated Hospital of Zhengzhou University

Lanzhou University Second Hospital

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Streptomyces formicae sp. nov., a novel actinomycete isolated from the head of Camponotus japonicus Mayr

Antonie van Leeuwenhoek (2015)

Lu Bai Chongxi Liu Lifeng Guo Chenyu Piao Zhilei Li

10.1007/s10482-015-0628-7

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Citations (25)

Elucidation of the ferrichrome siderophore biosynthetic pathway in albomycin-producing Streptomyces sp. ATCC 700974

Journal of Biological Chemistry (2023)

Zhilei Li Lang He Xia Wang Qingwen Huo Guosong Zheng

Sideromycins are a unique subset of siderophores comprising of a siderophore conjugated to an antimicrobial agent. The "Trojan horse" antibiotic albomycins are unique sideromycins consisting of a ferrichrome-type siderophore conjugated to a peptidyl nucleoside antibiotic. They exhibit potent antibacterial activities against many model bacteria and a number of clinical pathogens. Earlier studies have provided significant insight into the biosynthetic pathway of the peptidyl nucleoside moiety. We herein decipher the biosynthetic pathway of the ferrichrome-type siderophore in Streptomyces sp. ATCC 700974. Our genetic studies suggested that abmA, abmB, and abmQ are involved in the formation of the ferrichrome-type siderophore. Additionally, we performed biochemical studies to demonstrate that a flavin-dependent monooxygenase AbmB and an N-acyltransferase AbmA catalyze sequential modifications of L-ornithine to generate N5-acetyl-N5-hydroxyornithine. Three molecules of N5-acetyl-N5-hydroxyornithine are then assembled to generate the tripeptide ferrichrome through the action of a nonribosomal peptide synthetase AbmQ. Of special note, we found out that orf05026 and orf03299, two genes scattered elsewhere in the chromosome of Streptomyces sp. ATCC 700974, have functional redundancy for abmA and abmB, respectively. Interestingly, both orf05026 and orf03299 are situated within gene clusters encoding putative siderophores. In summary, this study provided new insight into the siderophore moiety of albomycin biosynthesis and shed light on the contingency of multiple siderophores in albomycin-producing Streptomyces sp. ATCC 700974.

Ferrichrome

Enterobactin

10.1016/j.jbc.2023.104573

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[Percutaneous transforaminal endoscopic discectomy combined with Coflex interspinous process dynamic reconstruction system in treatment of youth lumbar disc herniation].

Zhongguo xiufu chongjian waike zazhi (2017)

Yingjie Hao Tao Liu Lei Yu Caoyuan Ma Yawei Liu

To evaluate the effectiveness of percutaneous transforaminal endoscopic discectomy (PTED) combined with Coflex interspinous process dynamic reconstruction system for the treatment of youth lumbar disc herniation (LDH).

Lumbar disc herniation

Discectomy

10.7507/1002-1892.201608062

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Human NCF190H Variant Promotes IL-23/IL-17–Dependent Mannan-Induced Psoriasis and Psoriatic Arthritis

Antioxidants (2023)

Yanpeng Li Zhilei Li Kutty Selva Nandakumar Rikard Holmdahl

Recently, a major single nucleotide variant on the NCF1 gene, leading to an amino acid replacement from arginine to histidine at position 90 (NCF1R90H), associated with low production of reactive oxygen species (ROS), was found to be causative for several autoimmune diseases. Psoriasis in the skin (PsO) and psoriatic arthritis (PsA) were induced with mannan by intraperitoneal injection or epicutaneous application, evaluated by visual and histology scoring. Immunostaining was used to identify macrophages, NCF1, and keratinocytes. The population of immune cells was quantified by flow cytometry, gene expression was analyzed by RT-qPCR, and the JAK/STAT signaling pathway was investigated by immunohistochemical staining and western blot. We found that the low ROS responder NCF190H variant promotes PsO and PsA (the MIP model). The NCF190H-expressing mice had hyperactivated macrophages, expanded keratinocytes, and dramatically increased numbers of γδT17 cells with upregulated IL-17A, IL-23, and TNF-α. In addition, the JAK1/STAT3 signaling pathway was also upregulated in cells in the psoriatic skin tissues of Ncf190H mice. To summarize, a defined SNP (NCF1-339, also named NCF190H) was found to activate the IL-23/IL-17 axis and JAK-STAT signaling pathways, leading to hyperactivation of macrophages and keratinocytes and causing mouse psoriasis and psoriatic arthritis.

10.3390/antiox12071348

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Streptosporangium jiaoheense sp. nov. and Streptosporangium taraxaci sp. nov., actinobacteria isolated from soil and dandelion root (Taraxacum mongolicum Hand.-Mazz.)

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY (2016)

Junwei Zhao Lifeng Guo Zhilei Li Chenyu Piao Yao Li

Two novel actinobacteria, designated strains NEAU-Jh1-4T and NEAU-Wp2-0T, were isolated from muddy soil collected from a riverbank in Jiaohe and a dandelion root collected from Harbin, respectively. A polyphasic study was carried out to establish the taxonomic positions of these two strains. The phylogenetic analysis based on the 16S rRNA gene sequences of strains NEAU-Jh1-4T and NEAU-Wp2-0T indicated that strain NEAU-Jh1-4T clustered with Streptosporangium nanhuense NEAU-NH11T (99.32 % 16S rRNA gene sequence similarity), Streptosporangium purpuratum CY-15110T (98.30 %) and Streptosporangium yunnanense CY-11007T (97.95 %) and strain NEAU-Wp2-0T clustered with 'Streptosporangium sonchi ' NEAU-QS7 (99.39 %), 'Streptosporangium kronopolitis' NEAU-ML10 (99.26 %), 'Streptosporangium shengliense' NEAU-GH7 (98.85 %) and Streptosporangium longisporum DSM 43180T (98.69 %). Moreover, morphological and chemotaxonomic properties of the two isolates also confirmed their affiliation to the genus Streptosporangium. However, the low level of DNA-DNA hybridization and some phenotypic characteristics allowed the isolates to be differentiated from the most closely related species. Therefore, it is proposed that strains NEAU-Jh1-4T and NEAU-Wp2-0T represent two novel species of the genus Streptosporangium, for which the name Streptosporangium jiaoheense sp. nov. and Streptosporangium taraxaci sp. nov. are proposed. The type strains are NEAU-Jh1-4T (=CGMCC 4.7213T=JCM 30348T) and NEAU-Wp2-0T (=CGMCC 4.7217T=JCM 30349T), respectively.

Strain (injury)

10.1099/ijsem.0.001040

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Citations (12)

The systemic lupus erythematosus-associated NCF190H allele synergizes with viral infection to cause mouse lupus but also limits virus spread

Nature Communications (2025)

Yanpeng Li Ana Coelho Zhilei Li Malin Alsved Qixing Li

Abstract Studying how single nucleotide polymorphisms (SNPs) crosstalk with non-autologous factors to cause complex autoimmune diseases is challenging. An amino acid replacement in the neutrophil cytosolic factor 1 ( NCF1 -339/ NCF1 R90H ) leading to lower reactive oxygen species induction has been reported as the major SNP for systemic lupus erythematosus (SLE). Here we show that infection with the murine norovirus (MNV) contributes to the induction of lupus in Ncf1 90H mice. Mutant NCF1 90H upregulates the IFN-α/JAK1/STAT1 pathway in macrophages and anti-MNV-antibody production. In parallel, the MNV infection of NCF1 90H mice upregulates Toll-like receptor 7 in macrophages, plasmacytoid dendritic cells and B220 + splenocytes, thereby promoting germinal center formation and lupus-associated autoantibodies production. These compounded effects lead to protection against MNV infection but also glomeruloneph ritis with proteinuria and lupus arthritis in the absence of chemical inducers such as pristane. Our data thus suggest that this SLE-associated SNP, NCF1 90H , synergizes with MNV infection to induce the development of mouse lupus.

10.1038/s41467-025-56857-z

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Engineering nucleoside antibiotics toward the development of novel antimicrobial agents

The Journal of Antibiotics (2019)

Guoqing Niu Zhilei Li Pengju Huang Huarong Tan

10.1038/s41429-019-0230-8

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Citations (15)

Research on the potential mechanism of Chuanxiong Rhizoma on treating Diabetic Nephropathy based on network pharmacology

International Journal of Medical Sciences (2020)

Shanshan Hu Siteng Chen Zhilei Li Yuhang Wang Yong Wang

Background: Chuanxiong Rhizoma is one of the traditional Chinese medicines which have been used for years in the treatment of diabetic nephropathy (DN).However, the mechanism of Chuanxiong Rhizoma in DN has not yet been fully understood.Methods: We performed network pharmacology to construct target proteins interaction network of Chuanxiong Rhizoma.Active ingredients were acquired from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform.DRUGBANK database was used to predict target proteins of Chuanxiong Rhizoma.Gene ontology (GO) biological process analyses and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were also performed for functional prediction of the target proteins.Molecular docking was applied for evaluating the drug interactions between hub targets and active ingredients.Results: Twenty-eight target genes fished by 6 active ingredients of Chuanxiong Rhizoma were obtained in the study.The top 10 significant GO analyses and 6 KEGG pathways were enriched for genomic analysis.We also acquired 1366 differentially expressed genes associated with DN from GSE30528 dataset, including five target genes: KCNH2, NCOA1, KDR, NR3C2 and ADRB2.Molecular docking analysis successfully combined KCNH2, NCOA1, KDR and ADRB2 to Myricanone with docking scores from 4.61 to 6.28.NR3C2 also displayed good docking scores with Wallichilide and Sitosterol (8.13 and 8.34, respectively), revealing good binding forces to active compounds of Chuanxiong Rhizoma.Conclusions: Chuanxiong Rhizoma might take part in the treatment of DN through pathways associated with steroid hormone, estrogen, thyroid hormone and IL-17.KCNH2, NCOA1, KDR, ADRB2 and NR3C2 were proved to be the hub targets, which were closely related to corresponding active ingredients of Chuanxiong Rhizoma.

KEGG

DrugBank

Docking (animal)

Systems pharmacology

10.7150/ijms.47555

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Citations (10)

Research on the Potential Mechanism of Chuanxiong Rhizoma on Treating Diabetic Nephropathy Based on Network Pharmacology

Research Square (Research Square) (2020)

Shanshan Hu Siteng Chen Zhilei Li Yuhang Wang Yong Wang

Abstract Background: Chuanxiong Rhizoma is one of the traditional Chinese medicines which have been used for years in the treatment of diabetic nephropathy (DN). However, the mechanism of Chuanxiong Rhizoma in DN has not yet been fully understood. Methods: We performed network pharmacology to construct targeted proteins interaction network of Chuanxiong Rhizoma. Active ingredients were acquired from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform. DRUGBANK database was used to predict targeted proteins of Chuanxiong Rhizoma. Gene ontology (GO) biological process analyses and KEGG pathway enrichment analyses were also performed for functional prediction of the targeted proteins. Molecular docking was applied for evaluating the drug interactions between hub targets and active ingredients. Results: Twenty-eight targets fished by 6 active ingredients of Chuanxiong Rhizoma were obtained in the study. The top 10 significant GO analyses, as well as 6 KEGG pathways, were enriched for genomic analysis. We also acquired 1366 differentially expressed genes associated with DN from GSE30528 dataset, including five targeted genes: KCNH2, NCOA1, KDR, NR3C2 and ADRB2. Molecular docking analysis successfully combined KCNH2, NCOA1, KDR and ADRB2 to Myricanone with docking scores from 4.61 to 6.28. NR3C2 also displayed good binding forces (8.13 and 8.34) with Wallichilide and Sitosterol, revealing good binding forces to active compounds of Chuanxiong Rhizoma. Conclusions: Chuanxiong Rhizoma might take part in the treatment of DN through pathways associated with steroid hormone, estrogen, thyroid hormone and IL-17. KCNH2, NCOA1, KDR, ADRB2 and NR3C2 were proved to be the hub targets closely related to corresponding active ingredients of Chuanxiong Rhizoma.

DrugBank

KEGG

Docking (animal)

10.21203/rs.3.rs-18492/v1

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Citations (2)

Peucedanum praeruptorum Dunnの根圏から分離した放線菌の新種Streptomyces castaneus

Archives of Microbiology (2017)

Shuyu Zhou Zhilei Li Bai Lu Kai Yan Zhao Jun-wei

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