Xian Zou

Guangdong Academy of Agricultural Sciences

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Dewu Liu

South China Agricultural University

Yaokun Li

South China Agricultural University

Guangbin Liu

South China Agricultural University

Yongqing Guo

Capital University of Physical Education and Sports

Baoli Sun

South China Agricultural University

Ming Deng

South China Agricultural University

Chenglong Luo

Guangdong Academy of Agricultural Sciences

Jian Ji

Soochow University

Dingming Shu

Guangdong Academy of Agricultural Sciences

Hao Qu

Guangdong Academy of Agricultural Sciences

Cooperative Institutions

South China Agricultural University

Guangdong Academy of Agricultural Sciences

Key Laboratory of Guangdong Province

Central South University

Chinese Academy of Medical Sciences & Peking Union Medical College

Ministry of Agriculture and Rural Affairs

Soochow University

Nantong University

Chinese Academy of Sciences

First Affiliated Hospital of Soochow University

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Exploring the Rumen and Cecum Microbial Community from Fetus to Adulthood in Goat

Animals (2020)

Xian Zou Guangbin Liu Fanming Meng Linjun Hong Yaokun Li

The present study aimed to investigate the colonization process of epithelial bacteria attached to the rumen and intestinal tract tissue during the development of goats after birth. However, this process from fetus to adulthood was very limited. In goats, the rumen and cecum are two important fermentation organs, and it is important to study the acquisition and development of the neonatal microbiome, as well as the difference between these two organs. To characterize the microbial establishment and dynamic changes in the rumen and cecum from fetus to adulthood, we performed 16S rRNA gene sequencing for 106 samples from 47 individuals of nine pregnant mother-fetus pairs and 16 kids from birth up to 6 months. The diversity, structure and composition of the microbial communities were distinct between the rumen and cecum after birth, while they were similar in the fetal period. The study showed a rapid loss and influx of microbes at birth, followed by slight selection after drinking colostrum, and then a strong selection after weaning, suggesting that the establishment and dynamic fluctuations of the gut microbiome undergoes three distinct phases of microbiome progression in life: a conserved phase (during late pregnancy in the fetus), a transitional phase (newborn until weaning), and a stable phase (from weaning to adulthood). The results supported the view that microbes exist in the fetus, and revealed the establishment and dynamic fluctuations of the gut microbiome from fetus to adulthood in goats.

Cecum

10.3390/ani10091639

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Comprehensive Analysis of mRNAs and miRNAs in the Ovarian Follicles of Uniparous and Multiple Goats at Estrus Phase

Research Square (Research Square) (2019)

Xian Zou Tingting Lu Zhifeng Zhao Guangbin Liu Zhiquan Lian

Abstract Background Fertility is an important economic trait in production of meat goat, and follicular development plays an important role in fertility. Despite many mRNAs and microRNAs (miRNAs) have been found in playing critical roles in ovarian biological processes, the interactions between mRNAs and miRNAs in follicular development is not yet completely understood. In addition, less attention has been given to the single follicle (dominant or atretic follicle) in goat. The study was aimed to identify mRNAs, miRNAs and signaling pathways as well as their interaction networks in the ovarian follicles (large follicles and small follicles) of multiple and uniparous goats (Chuanzhong Black Goats) at estrus phase by using a deep RNA-sequencing (RNA-seq) method.Results The result showed that there were more large follicles in multiple than in uniparous goats ( P <0.05), while no difference was observed in small follicles between them ( P >0.05). For the small follicles of multiple and uniparous goats at estrus phase, 289 differentially expressed mRNAs (DEmRNAs) and 16 DEmiRNAs were identified; and for the large follicles, 195 DEmRNAs and 7 DEmiRNAs were identified. Ovarian steroidogenesis and steroid hormone biosynthesis were significantly enriched in small follicles, while ABC transporters and steroid hormone biosynthesis in large follicles. The results of qRT-PCR were generally consistent with the RNA-seq data. The mRNA-miRNA interaction network showed that CD36 (miR-122, miR-200a, miR-141), TNFAIP6 (miR-141, miR-200a, miR-182), CYP11A1 (miR-122), SERPINA5 (miR-1, miR-206, miR-133a-3p, miR-133b) and PTGFR (miR-182, miR-122) might be related to fertility, but need further verification.Conclusion This study provides the first identification of the DEmRNAs and DEmiRNAs as well as their interactions in the follicles of multiple and uniparous goats at estrus phase by using RNA-seq technology. These analyses provide new clues to uncover molecular mechanisms and signaling networks of goat reproduction which could be potentially used to increase ovulation rate and kidding rate in goat.

10.21203/rs.2.15819/v1

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Optimization of transfection conditions of chicken primordial germ cells.

Xian Zou Yanhua He Jingyi He Yan Wang Shu DingMing

To improve the transfection efficiency of chicken primordial germ cells (PGCs), the present study evaluated the plasmid dosage and cell number on the efficiencies of three transfection reagents (Lipofectamine 2000, 3000 and LTX & Plus Reagent). PGCs was isolated from embryonic gonads of Huiyang bearded chicken. After 60 days of culture in vitro, the cells were transfected by using Lipofectamine transfection reagents with piggyBac vectors coding for the green fluorescence protein (GFP). PGCs were passaged in culture and fluorescent cells were screened and selected by flow cytometry at three days after transfection. At three weeks post transfection, about 2000 cells were injected into the stage 16 Hamburger and Hamilton (HH) embryos and incubated until stage 30 HH. The results showed that Lipofectamine 3000 was the best for transfection of PGCs. The highest transfection efficiency of PGCs could be achieved with a combination of 3 μg plasmid, 4 μL Lipofectamine 3000 transfection reagent and 0.5×10 4PGCs cells. Flow cytometry analysis showed a 23.4% efficiency of stable transfection of PGCs using Lipofectamine 3000 with piggyBac vector, which was improved 2 times or more over current commonly used methods. After reinjecting PGCs into recipient chicken embryos, GFP-positive cells were observed in the gonads of the recipient chicken embryo by fluorescence microscopy. The study comprehensively evaluated the factors of transfection reagents, plasmid dosage and cell number to optimize the transfection of PGCs, thereby providing a foundation for the efficient preparation of transgenic and gene-edited chickens.

Lipofectamine

10.16288/j.yczz.20-212

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Author Correction: Transcriptome analysis of mRNA and miRNA in the development of LeiZhou goat muscles

Scientific Reports (2024)

Junjie Fu Jie Liu Xian Zou Ming Deng Guangbin Liu

10.1038/s41598-024-67345-7

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Advances in Studies on the Insecticidal Plant and its Applications and Prospects

Xian Zou

The advances of study on the plant-based pesticides and the insecticidal plant type, the acting way andeffective component of some insecticidal plants are introduced , of which includes more than 10 families such asMeliaceae, Celastraeae, Leguminosae, Compositae, Piperaceae, Stemonaceae, Thymelaeaceae etc. The prospect ofapplications and development of insecticidal plant is involved in the present work.

Thymelaeaceae

Agrochemical

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Identification of mRNAs and lncRNAs Involved in the Regulation of Follicle Development in Goat

Frontiers in Genetics (2020)

Zhifeng Zhao Xian Zou Tingting Lu Ming Deng Yaokun Li

Follicular development and maturation has a significant impact on goat reproductive performance, and it is therefore important to understand the molecular basis of this process. The importance of long non-coding RNAs (lncRNAs) in mammalian reproduction has been established, but little is known about the roles of lncRNAs in different follicular stages, especially in goats. In this study, RNA sequencing (RNA-seq) of large follicles (>10 mm) and small follicles (<3 mm) of Chuanzhong black goats was performed to investigate the regulatory mechanisms of lncRNAs and mRNAs in follicular development and maturation. A total of 8 differentially expressed lncRNAs (DElncRNAs) and 1,799 DEmRNAs were identified, and the majority of these were upregulated in small follicles. MRO , TC2N , CDO1 , and NTRK1 were potentially associated with follicular maturation. KEGG pathway analysis showed that the DEmRNAs involved in ovarian steroidogenesis ( BMP6 , CYP11A1 , CYP19A1 , 3BHSD , STAR , LHCGR , and CYP51A1 ) and cAMP signaling play roles in regulating follicular maturation and developmental inhibition respectively. Five target pairs of DElncRNA-DEmRNA, namely, ENSCHIT00000001255- OTX2 , ENSCHIT00000006005- PEG3 , ENSCHIT00000009455- PIWIL3 , ENSCHIT00000007977- POMP , and ENSCHIT00000000834- ACTR3 in co-expression analysis provide a clue in follicular development and maturation of lncRNA-mRNA interaction. Our findings provide a valuable resource for lncRNA studies, and could potentially provide a deeper understanding of the genetic basis and molecular mechanisms of goat follicular development and maturation.

KEGG

10.3389/fgene.2020.589076

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Transcriptomics-based identification and characterization of 11 CYP450 genes of <italic>Panax ginseng</italic> responsive to MeJA

Acta Biochimica et Biophysica Sinica (2018)

Xu Zeng Tiao Luo Jijia Li Li Gui Donghua Zhou

Cytochromes P450 (CYP450s), a superfamily of mono-oxygenases, are essential to generate highly functionalized secondary metabolites in plants and contribute to the diversification of specialized triterpenoid biosynthesis in eudicots. However, screening and identifying the exact CYP450 genes in ginsenoside biosynthesis is extremely challenging due to existence of large quantities of members in CYP450 superfamily. Therefore, to screen the CYP450 genes involved in ginsenoside biosynthesis, transcriptome dataset of Panax ginseng was created in our previous work using the technique of the next-generation sequencing. On the basis of bioinformatics analysis, 16 putative CYP450 genes with significant differential expression were screened from the dataset and submitted to GenBank, in which 11 of them have been cloned. Methyl jasmonate (MeJA) was used as an elicitor to analyze the expression profiles of candidate CYP450 genes by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The results of qRT-PCR analysis revealed that the expression of some CYP450 genes were strongly induced by MeJA and showed different transcription levels at different treatment time points. Homology analysis indicated that each putative CYP450 protein of P. ginseng has a conserved domain consisting of E-E-R-F-P-R-G. The CYP450 genes were screened and cloned here to enrich the resources of CYP450 genes, and the results of bioinformatics analysis provided a foundation to further identify the function of CYP450s involved in ginsenoside biosynthesis. Furthermore, this study facilitated the construction of microbial cell factories for increasing the production of ginsenosides by means of metabolic engineering.

Methyl jasmonate

10.1093/abbs/gmy120

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<p>Long Noncoding RNA LINC00460 Modulates MMP-9 to Promote Cell Proliferation, Invasion and Apoptosis by Targeting miR-539 in Papillary Thyroid Cancer</p>

Cancer Management and Research (2020)

Xian Zou Zhi Guo Qun Li Pei Song Wang

Increasing evidence shows that Long non-coding RNAs (lncRNAs) involve in the development and progression processes of various cancers, including papillary thyroid cancer (PTC). In this study, we focused on the regulation function of lncRNA LINC00460 in the development of PTC.Expression of LINC00460 was detected using quantitative real-time PCR (qRT-PCR) and Western blot assay. Cell proliferation, cell apoptosis and cell invasion were determined through CCK-8 assay, flow cytometry, and Transwell assay, respectively. In addition, target sites were detected by the dual-luciferase reporter gene assay.LINC00460 expression was markedly up-regulated in PTC tissues and cells compared to their corresponding controls by quantitative real-time PCR (qRT-PCR). Meanwhile, LINC00460 knockdown notably inhibited the proliferation capacity, accelerated the apoptosis and down-regulated the invasion-related proteins (MMP-2, MMP-9, ZEB1) expression. In addition, bioinformatics tools predicted that miR-539 both targeted with the 3'-UTR of LINC00460 and MMP-9, which was confirmed by luciferase reporter assay and Western blot.These findings indicated that LINC00460 can modulate MMP-9 expression to promote cell proliferation, invasion and apoptosis through targeting miR-539, suggesting act as an oncogenic RNA in PTC and provide a new therapeutic perspective.

10.2147/cmar.s222085

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Research progress on pharmacological activities of chelidonine

Xiandai yaowu yu linchuang (2014)

Xian Zou

Chelidonine, a benzophen anthridine alkaloid, is one of the main effective constituents of Chelidonium majus, which is applied as a painkiller in clinical treatment. The further studies on the pharmacological activities in recent years have demonstrated that chelidonine plays a role in antitumor, analgesic, antibacterial, spasmolysis activities, etc. And the antitumor activity is paid more and more attention. In this paper, research progress on pharmacological activities and mechanisms of chelidonine home and abroad in recent years are reviewed, which could provide a theoretical basis for the further study and application of C. majus.

Chelidonium

Sanguinarine

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[Comparison of therapeutic effects between acupoint-injection and intramuscular-injection of Bacillus Calmette-Guerin polysaccharide nucleic acid for bronchial asthma].

PubMed (2015)

Xian Zou Jing Zhu Xin-zhong Kang Wei-yong Hu Kang Wang

To observe the curative effect of acupoint-injection and intramuscular-injection of Bacillus Calmette-Guerin (BCG) Polysaccharide Nucleic Acid for bronchial asthma.Sixty patients with bronchial asthma were equally randomized into acupoint-injection group and intramuscular-injection group. For patients of the acupoint-injection group, 2 mL of BCG was injected into bilateral Feishu (BL 13, 1 mL for one side) once per day in the first 15 days, and once every other day in the rest 2.5 months except weekends. Intramuscular-injection was conducted at the lateral sites of the left or right buttock, 2 mL/time for each site, and the injection frequency was the same to that of the acupoint-injection. The therapeutic effect was assessed according to the standards of Guide Principles for Clinical Research of New Chinese Herbal Drugs (2002) and Asthma Group of Breathing Diseases of China Medical Association (2008). Changes of FEV 1 (forced expiratory volume in the first second)/FVC (forced vital capacity) and PEF% (peak expiratory flow) were detected using a pulmonary function detector. Serum IgA, IgM, IgG and IgE contents were assayed by using an autonomic biochemical analyzer.After 3 months' treatment, the scores of patients' symptoms and signs and serum IgE and IgG levels were significantly decreased in both muscular-injection and acupoint-injection groups (P<0.01), while asthma controlled test (ACT) scores FEV 1/FVC% and PEF% values were considerably increased in both groups compared to pre-treatment in the same one group (P<0.01). The effects of acupoint-injection were markedly superior to those of the intramuscular-injection in reducing clinical symptom-sign score, and serum IgE content, and in up-regulating ACT score, FEV 1/FVC% and PEF% levels (P<0.05, P<0.01). There were no significant differences in serum IgG, IgA and IgM levels between the two groups 3 months following the treatment (P>0.05).Acupoint-injection of BCG polysaccharide nucleic acid can effectively improve bronchial asthma patients' clinical symptoms and sings and pulmonary function, which may be closely associated with its function in down-regulating serum IgE and IgG levels.

Intramuscular injection

Vital capacity

Therapeutic effect

Subcutaneous injection

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