Isabelle Denis

Physiologie de la Nutrition et du Comportement Alimentaire

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Monique Lavialle

Département Alimentation Humaine

Gaëlle Champeil‐Potokar

Physiologie de la Nutrition et du Comportement Alimentaire

Sylvie Vancassel

Université de Bordeaux

Philippe Guesnet

Société Française d'Endoscopie Digestive

Olivier Rampin

Physiologie de la Nutrition et du Comportement Alimentaire

Alain Pointillart

Centre de Recherche en Nutrition Humaine Rhône-Alpes

Marie Hennebelle

Wageningen University & Research

Gaelle Champeil Potokar

Neurobiologie de l'Olfaction et Modélisation en Imagerie

Jean Marc J. M. Alessandri

Institut National de la Recherche Agronomique

Vincent Bombail

Scotland's Rural College

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Physiologie de la Nutrition et du Comportement Alimentaire

Centre National de la Recherche Scientifique

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Inserm

Institut National de la Recherche Agronomique

Université Paris-Saclay

AgroParisTech

Université Paris Cité

Université Claude Bernard Lyon 1

Neurobiologie de l'Olfaction et Modélisation en Imagerie

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Évaluation du rôle biais cognitifs des parents dans la transmission intergénérationnelle de l'anxiété chez de jeunes enfants

Isabelle Denis

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Rôle des omega-3 dans la plasticité cérébrale : des astrocytes aux rythmes circadiens

HAL (Le Centre pour la Communication Scientifique Directe) (2004)

Isabelle Denis Saba Aïd Jean Marc J. M. Alessandri Gaëlle Champeil‐Potokar Philippe Guesnet

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Effet de la supplémentation en DHA (acide docosahexaéonique) sur la composition lipidique membranaire d'astrocytes en culture

HAL (Le Centre pour la Communication Scientifique Directe) (2001)

Gaëlle Champeil‐Potokar Isabelle Denis Bénédicte Goustard‐Langelier Monique Lavialle Jean Marc J. M. Alessandri

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CRE mice exhibit hyperactive and impulsive behavior affecting their learning and retention performances

Frédéric Desor Aseel El Hajj Améziane Herzine Fathia Djelti Vincent Bombail

10.1101/2024.02.27.582228v1

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CRE mice exhibit hyperactive and impulsive behavior affecting their learning and retention performances

bioRxiv (Cold Spring Harbor Laboratory) (2024)

Frédéric Desor Aseel El Hajj Améziane Herzine Fathia Djelti Vincent Bombail

Abstract CRE recombinase is a protein that recognizes and mediates site-specific recombination between loxP site sequences. The Cre/loxP recombination system has become a useful tool for genetic manipulation. Spatial regulation of recombination can be achieved by using cell type-specific promoters that drive expression of CRE in the tissue of interest. The temporal regulation can be obtained with CreER recombinase, which consists of Cre fused to mutated hormone-binding domain of the estrogen receptor (ER). In the more improved versions of the construct, the CRE - mediated gene regulation can be controlled both spatially and temporally, by combining tissue-specific expression of a CreER recombinase with its tamoxifen-dependent activity. We recently generated and characterized an astrocyte specific mutant of the lipolysis-stimulated lipoprotein receptor lsr gene by crossing Glast ERT2 mice with floxed lsr mice (El Hajj et al., 2022). During the behavioral analysis of generated mice, we identified specific hyperactive traits in the Glast ERT2 mice (CRE mice) that prevented them from being used as a control group. Here we further assessed the hyperactive trait of those CRE mice using a battery of behavioral tests. We showed that CRE mice exhibited hyperactive behavior combined with attention-deficit, sleep disturbance and impulsivity that affect their learning and memorization performances. These mice may therefore serve as a model to study attention deficit / hyperactivity disorder. Our work also pointed out the need for proper behavioral analysis of control groups in transgenic animal generation to avoid misinterpretation and misattribution of behavioral traits.

Cre recombinase

10.1101/2024.02.27.582228

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Influence des apports alimentaires en Oméga-3 sur les processus cognitifs et la physiologie cérébrale

HAL (Le Centre pour la Communication Scientifique Directe) (2011)

Isabelle Denis Sylvie Vancassel Monique Lavialle

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Influence of high concentrations of extracellular calcium on the proliferation and differentiation of porcine osteoblasts in culture

annales de biologie animale biochimie biophysique (1997)

E. Eklou-Kalonji Isabelle Denis Alain Pointillart

10.1051/rnd:19970382

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Astrocytes in culture require docosahexaenoic acid to restore the n‐3/n‐6 polyunsaturated fatty acid balance in their membrane phospholipids

Journal of Neuroscience Research (2003)

Gaëlle Champeil‐Potokar Isabelle Denis Bénédicte Goustard‐Langelier Jean‐Marc Alessandri Philippe Guesnet

Abstract Docosahexaenoic acid (DHA), the main n‐3 polyunsaturated fatty acid (PUFA) in membranes, is particularly abundant in brain cells. Decreased cerebral concentrations of DHA, resulting from dietary n‐3 deficiency, are associated with impaired cognitive function. Because the cellular causes of this impairment are still unknown, we need in vitro models that mimic the variations in n‐3/n‐6 PUFA seen in vivo. We have compared the PUFA profiles of hamster astrocytes cultured in medium supplemented with long‐chain PUFA [DHA and/or arachidonic acid (AA)] with those of brain tissue from hamsters fed an n‐6/n‐3 PUFA‐balanced diet or one lacking n‐3 PUFA. Astrocytes were obtained from the brain cortex of newborn hamsters and cultured in minimum essential medium + 5% fetal calf serum (FCS) supplemented with DHA and/or AA for 10 days. The astrocytes cultured in medium + FCS had low n‐3 PUFA contents, comparable to those of brain tissue from hamsters fed an n‐3‐deficient diet. We have shown that astrocytes grown in medium supplemented with DHA and/or AA, plus α‐tocopherol to prevent lipid peroxidation, incorporated large amounts of these long‐chain PUFA, so that the n‐6/n‐3 PUFA compositions of the phosphatidylethanolamine and phosphatidylcholine, the two main classes of membrane phospholipids, were greatly altered. Astrocytes cultured in medium plus DHA had a more physiological n‐3 status, grew better, and retained their astrocyte phenotype. Thus astrocytes in culture are likely to be physiologically relevant only when provided with adequate DHA. This reliable method of altering membrane phospholipid composition promises to be useful for studying the influence of n‐6/n‐3 imbalance on astrocyte function. © 2003 Wiley‐Liss, Inc.

Phosphatidylethanolamine

10.1002/jnr.10817

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Cadillac

Oxford Art Online (2003)

Isabelle Denis

10.1093/gao/9781884446054.article.t012940

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La douleur, un phénomène complexe

Guillaume Foldes‐Busque Isabelle Denis

10.1515/9782763734156-003

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