Anastasia Krivoruchko

North-Caucasus Federal University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Jens Nielsen

Chalmers University of Technology

Verena Siewers

Chalmers University of Technology

Kenneth B. Storey

Carleton University

O. A. Yatsyk

Federal Agrarian Scientific Center of the North-East named after NV Rudnitsky

Yongjin J. Zhou

Dalian Institute of Chemical Physics

A. A. Kanibolotskaya

North-Caucasus Federal University

Yun Chen

Zhejiang Chinese Medical University

АА

А. А. Лиховид

North-Caucasus Federal University

Jiufu Qin

Sichuan University

T. Yu. Saprikina

North-Caucasus Federal University

Cooperative Institutions

Chalmers University of Technology

Stavropol State Agrarian University

North-Caucasus Federal University

Technical University of Denmark

Novo Nordisk Foundation

Federal Agrarian Scientific Center of the North-East named after NV Rudnitsky

Eskişehir City Hospital

Anadolu University

Agricultural Research Center

Stavropol Research Institute of Animal Production and Feed Production

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Activation of antioxidant defenses in response to freezing in freeze-tolerant painted turtle hatchlings

Biochimica et Biophysica Acta (BBA) - General Subjects (2010)

Anastasia Krivoruchko Kenneth B. Storey

Hatchling

Painted turtle

Glutathione reductase

GSTP1

10.1016/j.bbagen.2010.03.015

Cite

Citations (52)

Expanding the product portfolio of fungal type I fatty acid synthases

Nature Chemical Biology (2017)

Zhiwei Zhu Yongjin J. Zhou Anastasia Krivoruchko Martin Grininger Zongbao K. Zhao

Acyl carrier protein

Heterologous expression

Heterologous

10.1038/nchembio.2301

Cite

Citations (105)

Screening of 2A peptides for polycistronic gene expression in yeast

FEMS Yeast Research (2018)

Tatiana M. Souza-Moreira Clara Navarrete Xin Chen Cleslei Fernando Zanelli Sandro Roberto Valentini

A complexity of pathway expression in yeast compared to prokaryotes is the need for separate promoters and terminators for each gene expressed. Single transcript expression and separated protein production is possible via the use of 2A viral peptides, but detailed characterization to assess their suitability and applications is needed. The present work aimed to characterize multiple 2A peptide sequences to determine suitability for metabolic engineering applications in Saccharomyces cerevisiae. We screened 22 peptides placed between fluorescent protein sequences. Cleaving efficiency was calculated by western blot intensity of bands corresponding to the cleaved and uncleaved forms of the reporter. Three out of the 22 sequences showed high cleavage efficiency: 2A peptide from Equine rhinitis B virus (91%), Porcine teschovirus-1 (85%) and Operophtera brumata cypovirus-18 (83%). Furthermore, expression of the released protein was comparable to its monocistronic expression. As a proof-of-concept, the triterpene friedelin was successfully produced in the same yeast strain by expressing its synthase with the truncated form of HMG1 linked by the 2A peptide of ERBV-1, with production titers comparable to monocistronic expression (via separate promoters). These results suggest that these peptides could be suitable for expression and translation of multiple proteins in metabolic engineering applications in S. cerevisiae.

10.1093/femsyr/foy036

Cite

Citations (59)

Yeast cell factories for production of taxadiene

Hülya Karaca Anastasia Krivoruchko Jens Nielsen

27th International Conference on Yeast Genetics and Molecular Biology (ICYGMB) -- SEP 06-12, 2015 -- Fondazione Edmund Mach, Levico Terme, ITALY

Source

Cite

Citations (0)

Functional pyruvate formate lyase pathway expressed with two different electron donors in Saccharomyces cerevisiae at aerobic growth

FEMS Yeast Research (2015)

Yiming Zhang Zongjie Dai Anastasia Krivoruchko Yun Chen Verena Siewers

Pyruvate formate lyase (PFL) is characterized as an enzyme functional at anaerobic conditions, since the radical in the enzyme's active form is sensitive to oxygen. In this study, PFL and its activating enzyme from Escherichia coli were expressed in a Saccharomyces cerevisiae strain lacking pyruvate decarboxylase and having a reduced glucose uptake rate due to a mutation in the transcriptional regulator Mth1, IMI076 (Pdc−MTH1-ΔT ura3–52). PFL was expressed with two different electron donors, reduced ferredoxin or reduced flavodoxin, respectively, and it was found that the coexpression of either of these electron donors had a positive effect on growth under aerobic conditions, indicating increased activity of PFL. The positive effect on growth was manifested as a higher final biomass concentration and a significant increase in transcription of formate dehydrogenases. Among the two electron donors reduced flavodoxin was found to be a better electron donor than reduced ferredoxin.

Flavodoxin

Pyruvate decarboxylase

Exergonic reaction

Electron acceptor

10.1093/femsyr/fov024

Cite

Citations (18)

Modular pathway rewiring of Saccharomyces cerevisiae enables high-level production of L-ornithine

Nature Communications (2015)

Jiufu Qin Yongjin J. Zhou Anastasia Krivoruchko Mingtao Huang Lifang Liu

Abstract Baker’s yeast Saccharomyces cerevisiae is an attractive cell factory for production of chemicals and biofuels. Many different products have been produced in this cell factory by reconstruction of heterologous biosynthetic pathways; however, endogenous metabolism by itself involves many metabolites of industrial interest, and de-regulation of endogenous pathways to ensure efficient carbon channelling to such metabolites is therefore of high interest. Furthermore, many of these may serve as precursors for the biosynthesis of complex natural products, and hence strains overproducing certain pathway intermediates can serve as platform cell factories for production of such products. Here we implement a modular pathway rewiring (MPR) strategy and demonstrate its use for pathway optimization resulting in high-level production of L -ornithine, an intermediate of L -arginine biosynthesis and a precursor metabolite for a range of different natural products. The MPR strategy involves rewiring of the urea cycle, subcellular trafficking engineering and pathway re-localization, and improving precursor supply either through attenuation of the Crabtree effect or through the use of controlled fed-batch fermentations, leading to an L -ornithine titre of 1,041±47 mg l −1 with a yield of 67 mg (g glucose) −1 in shake-flask cultures and a titre of 5.1 g l −1 in fed-batch cultivations. Our study represents the first comprehensive study on overproducing an amino-acid intermediate in yeast, and our results demonstrate the potential to use yeast more extensively for low-cost production of many high-value amino-acid-derived chemicals.

Bioproduction

Metabolic pathway

Metabolic Engineering

10.1038/ncomms9224

Cite

Citations (107)

MOESM2 of Adaptive mutations in sugar metabolism restore growth on glucose in a pyruvate decarboxylase negative yeast strain

Figshare (2015)

Yiming Zhang Guodong Liu Martin K. M. Engqvist Anastasia Krivoruchko BjĂśrn HallstrĂśm

Additional file 2. Â Genome sequencing results of Pdc negative strain E1 and its evolved strains E1A, E1B, E1C.

Pyruvate decarboxylase

Strain (injury)

Carbohydrate Metabolism

10.6084/m9.figshare.c.3596153_d3

Cite

Citations (0)

Turtle anoxia : biochemistry and gene regulation in an anaerobic extremist

Anastasia Krivoruchko

lnternet, preter, distribuer et vendre des theses partout dans le monde, a des fins commerciales ou autres, sur support microforme, papier, electronique et/ou autres formats.

10.22215/etd/2010-09691

Cite

Citations (1)

Anoxia-responsive regulation of the FoxO transcription factors in freshwater turtles, Trachemys scripta elegans

Biochimica et Biophysica Acta (BBA) - General Subjects (2013)

Anastasia Krivoruchko Kenneth B. Storey

Transcription

10.1016/j.bbagen.2013.06.034

Cite

Citations (30)

Genome-wide association study (GWAS) with productivity in Romanov sheep breed

Proceedings of the National Academy of Sciences of Belarus Agrarian Series (2021)

Anastasia Krivoruchko O. A. Yatsyk T. Yu. Saprikina D. D. Petukhova

Genetic technologies used in breeding of small ruminants requires searching for new molecular markers of productive traits. The most effective for this is genome-wide association study (GWAS) of single nucleotide polymorphisms (SNP) with economically valuable traits. The paper presents results of study of associations of the frequency of single nucleotide polymorphisms with a rank assessment according to complex of productive traits (super-elite) in Romanov sheep using DNA biochips Ovine Infinium HD BeadChip 600K. Eleven SNPs have been found having significant correlation with the animals belonging to the “super-elite” group. Five substitutions are located in the genes introns, six are related to intergenic polymorphisms. The highest reliability of association with productivity was observed in substitution rs410516628 (р = 3,14 · 10 -9 ) located on the 3rd chromosome. Substitution rs422028000 on 2nd chromosome differs with the fact that in the “super-elite” group it was found in 90 % of haplotypes. Polymorphisms rs411162754 (1st chromosome) and rs417281100 (10th chromosome) in our study turned out to be the rarest – only in “super-elite” group and only in a quarter of haplotypes. The genes located near the identified SNPs are mainly associated with metabolic and regulatory processes. Our study has identified several new candidate genes with polymorphism probably associated with the ranking in terms of productivity in Romanov sheep: LTBP1, KCNH8, LMX1B, ZBTB43, MSRA, CHPF, PID1 and DNER. The results obtained create a theoretical basis for further study of candidate genes affecting implementation of phenotypic traits in Romanov sheep. The revealed polymorphisms associated with the productive traits of sheep can be used in practical breeding as molecular and genetic markers for selection of parental pairs.

Genome-wide Association Study

Candidate gene

Genetic Association

10.29235/1817-7204-2021-59-1-71-80

Cite

Citations (1)