AIM To study effect of soybean isoflavones (SI) on spleen in radiated mice. METHODS 90 male mice were randomly divided into control group, radiated group, radiated plus 0.5% dose SI group. After 2-week feeding, the mice received 4.0 Gy 137Cs gamma-radiation, the cell cycles, cell apoptosis and proliferation on the spleen and the spleen index were observed in radiated after 12 h, 24 h, 1 week and 2 weeks. RESULTS After the mice were radiated, the spleen were significantly atrophy, the rate of the cell apoptosis and the cell cycles of G0-G1 phase in splenocytes were significantly increased (P < 0.01), the cell cycles rate of S phase and the proliferation index were significantly decreased in spleen (P < 0.05). Compared with radiated group, the spleen atrophy and the rate of the cell cycles of G0-G1 phase were significantly decreased (P < 0.05), and the cell cycles of G2-M phase and the proliferation index were significantly increased (P < 0.05) in the mice supplied 0.5% soybean isoflavones. CONCLUSION The soybean isoflavones could significantly increase spleen radioprotective effect in mice.
ms-Tetra (p-alkylthiophenyl) porphyrins and ms-tetra (o-alkylthiophenyl) porphyrins(alkyl=methyl, ethyl, propyl) were synthesized by refluxing the mixture of pyrrole,and para or ortho (alkylthio) benzaldehydes in propionic acid or in acetic acid. Some metal complexes of these porphyrins were prepated by refluxing the mixture of the porphyrin and the corresponding metal salt in acetic acid or in DMF. All the porphyrins described were identified by visible spectrophotometry, nuclear magnetic resonance spectroscopy and elemental analysis.
ArfGAP with SH3 domain, ankyrin repeat and PH domain 3 (ASAP3), previously known as ACAP4, DDEFL1 and UPLC1, is considered to be an important regulator in cancer cell migration/invasion and actin-based cytoskeletal remodeling. However, the underlying mechanisms through which ASAP3 mediates these processes are not well-elucidated. This study reported that in certain types of cancer cells, loss of ASAP3 suppressed cell migration/invasion, in part by destabilizing γ-actin-1 (ACTG1), a cytoskeletal protein considered to be an integral component of the cell migratory machinery, essential for the rearrangement of the dynamic cytoskeletal networks and important in diseases, such as brain malformation, hearing loss and cancer development. The data, for the first time, link ASAP3 with ACTG1 in the regulation of cytoskeletal maintenance and cell motility.
Evaluation of the endoscopic features of Crohn's disease (CD) and ulcerative colitis (UC) is the key diagnostic approach in distinguishing these two diseases. However, making diagnostic differentiation of endoscopic images requires precise interpretation by experienced clinicians, which remains a challenge to date. Therefore, this study aimed to establish a convolutional neural network (CNN)-based model to facilitate the diagnostic classification among CD, UC, and healthy controls based on colonoscopy images.A total of 15,330 eligible colonoscopy images from 217 CD patients, 279 UC patients, and 100 healthy subjects recorded in the endoscopic database of Tongji Hospital were retrospectively collected. After selecting the ResNeXt-101 network, it was trained to classify endoscopic images either as CD, UC, or normal. We assessed its performance by comparing the per-image and per-patient parameters of the classification task with that of the six clinicians of different seniority.In per-image analysis, ResNeXt-101 achieved an overall accuracy of 92.04% for the three-category classification task, which was higher than that of the six clinicians (90.67, 78.33, 86.08, 73.66, 58.30, and 86.21%, respectively). ResNeXt-101 also showed higher differential diagnosis accuracy compared with the best performing clinician (CD 92.39 vs. 91.70%; UC 93.35 vs. 92.39%; normal 98.35 vs. 97.26%). In per-patient analysis, the overall accuracy of the CNN model was 90.91%, compared with 93.94, 78.79, 83.33, 59.09, 56.06, and 90.91% of the clinicians, respectively.The ResNeXt-101 model, established in our study, performed superior to most clinicians in classifying the colonoscopy images as CD, UC, or healthy subjects, suggesting its potential applications in clinical settings.
The curriculum of Communitity Health Nursing has fine operation and practicability.Aiming at this point, the situational teaching method is used in teaching.This method is uniquely excellent in arousing students enthusiasm of learning,improving students' ability to integrate theory with practice, cultivating students' creative thinking and team spirit. The teaching effect has been proved correct in our practice.
Key words:
Communitity health nursing; Nursing education; Situational teaching
Objective To explore the application of question-based lectures made by students in teaching chapter of nuclear chemical and biological weapon damage among field military nursing.Methods The participants were junior students of four-year undergraduate program.Questions on emergency treatment and care of chemical and biological weapon damage were proposed after a lecture by teacher.Students were divided into three groups after class and each group was assigned to a lecture on emergency treatment and care of one kind of weapon damage.Students had to collect information and prepare PPT for the next lecture.After lectures given by students at next class,discussion on the lectures was organized and comments were given by teachers.Results All students approved this kind of teaching activities,97.44％ believed that it can arouse their interest in study,94.87％ participated in the collection of data,89.74％ took part in the discussion,and 87.18％ obtained the sense of achievements.Conclusion Question-based lectures made by students are helpful in stimulating their interest in learning,cultivating their abilities of acquiring and applying knowledge.The teaching activities achieve the preferable results through proper application and meticulous organization.
Key words:
Field military nursing; Student-made lecture; Teaching
Objective: To infer which morphologic change of gastric mucosa has more probability to develop into dysplasia or cancer by detecting the expressions of regenerating gene 4 (Reg4) and Ki67 in different morphologies of intestinal metaplasia in gastric antrum. Methods: Immunohistochemistry and semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR) were used to examine the expression levels of Reg4 and Ki67 proteins and mRNAs in mucosal biopsy specimens of 385 cases of different types of gastric disease. Results: In the gastric mucosal biopsy specimens each from nonatrophic gastritis (NAG), atrophic gastritis (AG), atrophic gastritis with intestinal metaplasia displaying superficial depressed mucosa lesions (DIM), atrophic gastritis with intestinal metaplasia displaying superficial elevated mucosa lesions (EIM) and gastric cancer (GC), the positive rates of Reg4 protein was 12.3%, 26.7%, 43.6%, 93.6% and 31.5%, respectively; the positive rate of Reg4 protein of EIM was significantly different from those of NAG, AG, DIM and GC (P＜0.05). The positive rates of Ki67 protein were 14.8%, 18.7%, 23.1%, 69.2% and 71.2% in NAG, AG, DIM, EIM and GC, respectively; the positive rate of Ki67 protein in EIM was significantly different from those of NAG, AG and DIM (P＜0.05) but not different from that of GC (P＞0.05). The expressions of Reg4 and Ki67 mRNAs in EIM were also significantly higher than those in DIM. Conclusion: The expression levels of Reg4 and Ki67 in EIM were higher than those in DIM, which suggests that EIM may have more probability to develop into dysplasia or cancer than DIM, and DIM may become a more valuable specimen for gastric biopsy. DOI:10.3781/j.issn.1000-7431.2011.10.011
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