NHL protein family is evolutionarily conserved in either vertebrates or invertebrates. It has been extensively studied in mammals for the function of regulating cell proliferation and differentiation. Brat, known as the homologue of TRIM in insects, may have the same function. In this paper, we identified and characterized BmBrat in silkworm. The gene expression profile indicated that BmBrat was expressed in all tissues and the expression was especially high in ovary and head at larval stage. The expression profile was also detected at different stages of embryo development, and reached a peak at 4th and 5th days of the embryonic period. Subcellular localization of BmBrat in hemocytes revealed that it was specifically expressed in cytoplasm. Over-expression of BmBrat in silkworm significantly reduced the rate of cell proliferation by BrdU staining. This finding suggested that BmBrat played an important role in cell processes.

Bombyx

10.25431/1824-307x/isj.v15i1.149-157

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Genome-wide association analysis of aluminum tolerance related traits in rapeseed (Brassica napus L.) during germination

Genetic Resources and Crop Evolution (2020)

Huanhuan Gao Ye Sang Jiayi Wu Liuyan Wang Ruili Wang

Tassel

Candidate gene

Genome-wide Association Study

Genetic Association

SNP

10.1007/s10722-020-00989-2

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Citations (10)

Direct Exposure of Wheat Seeds to High-Voltage Electrostatic Fields Adversely Affects the Performance ofSitobion avenae(Hemiptera: Aphididae)

Journal of Economic Entomology (2016)

Kun Luo Zhu A. Cao Rui Gao Juan He Guangyu Li

Exposing seeds to a high-voltage electrostatic field (HVEF) may influence the performance of herbivores and improve the germination rate by inducing biological and physiological changes in plants. In the current study, an age-stage, two-sex life table was established to evaluate the effects from directly exposing seeds to HVEFs on the performance of apterous Sitobion avenae (Fabricius) reared on winter wheat. We treated the wheat seeds by exposing them to an HVEF for 20 min at three intensities: 2, 4, or 6 KV/cm. Controls received no treatment (0 KV/cm). The results indicated that the parameters of the net reproductive rate (R0), the intrinsic rate of increase (r), and the mean generation span (T) of S. avenae were significantly reduced by HVEFs through multiple generations. In addition, the age-specific survival rate (lx) and comparison with the results of a Weibull equation analysis suggested that S. avenae had the shortest life span when seeds were exposed to an HVEF at 4 KV/cm. Overall, these findings indicated that direct exposure of wheat seeds to an HVEF at 4 KV/cm could adversely affect the performance of S. avenae.

Sitobion avenae

Population Dynamics

10.1093/jee/tow227

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Functional roles of Arabidopsis CKRC2/YUCCA8 gene and the involvement of PIF4 in the regulation of auxin biosynthesis by cytokinin

Scientific Reports (2016)

Dong‐Wei Di Lei Wu Li Zhang Chen-Wei An Tianzi Zhang

Abstract Auxin and cytokinin (CK) are both important hormones involved in many aspects of plant growth and development. However, the details of auxin biosynthesis and the interaction between auxin and CK are still unclear. Isolation and characterization of an auxin deficient mutant c yto k inin induced root c urling 2 (ckrc2 ) in this work reveal that CKRC2 encodes a previously identified member of YUCCA (YUC) flavin monooxygenase-like proteins (YUC8). Our results show that, like other YUCs, CKRC2/YUC8 is a rate-limiting enzyme for catalyzing the conversion of indole-3-pyruvic acid (IPyA) to indole-3-acetic acid (IAA), acting downstream of CKRC1/TAA1 in the IPyA pathway. Here we show that the transcription of both CKRC1/TAA and CKRC2 / YUC8 can be induced by CK and that the phytochrome-interacting factor 4 (PIF4) is required for this upregulation. Transcription of PIF4 itself is induced by CK via the AHKs-ARR1/12 signalling pathway. These results indicate that PIF4 plays an essential role in mediating the regulatory effect of CK on the transcriptions of CKRC1 and CKRC2 genes in the IPyA pathway of auxin biosynthesis.

Transcription

10.1038/srep36866

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Citations (39)

Transcriptome profiling and phytohormone responses of Arabidopsis roots to different ambient temperatures

Journal of Plant Interactions (2019)

Qionghui Fei Liyuan Liang Fan Li Li Zhang Youxia Li

Ambient temperatures influence plant growth and development, however very little is known about changes in root growth in response to ambient temperature change. Here, we performed transcriptome profiling and compared the differences in gene expression at lower and higher temperatures compared with normal plant growth temperatures. Our analysis of the biological processes and molecular functions regulated by differentially expressed genes revealed that low temperature upregulated carbohydrate metabolism and transmembrane transport, and downregulated signal transduction and defense. High temperature upregulated metabolic processes, transport, and auxin biosynthesis, and downregulated catabolic processes. We found that increased temperature specifically affected the levels of Arabidopsis response regulators, ARR1 and ARR12, to decrease cytokinin signaling, altered the level of the brassinosteroid receptor BRI1 to downregulate brassinosteroid signaling, and changed the level of the gibberellin receptor DELLA to upregulate gibberellin signaling and mediate root elongation. These data contribute to our knowledge of how root growth adapts to elevated ambient temperature under climate warming.

Brassinosteroid

Plant hormone

10.1080/17429145.2019.1634770

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CCDC 972605: Experimental Crystal Structure Determination

The Cambridge Structural Database (2015)

Mingxue Sun Huanhuan Gao Jie Zhao Lei Zhang Kai Xiao

An entry from the Cambridge Structural Database, the world’s repository for small molecule crystal structures. The entry contains experimental data from a crystal diffraction study. The deposited dataset for this entry is freely available from the CCDC and typically includes 3D coordinates, cell parameters, space group, experimental conditions and quality measures.

Crystal (programming language)

10.5517/cc11n2c4

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AtHSPR functions in gibberellin-mediated primary root growth by interacting with KNAT5 and OFP1 in Arabidopsis

Plant Cell Reports (2023)

Guoqiang Yuan Yuke Lian Junmei Wang Taibi Yong Huanhuan Gao

Gibberellic acid

Paclobutrazol

Lateral root

10.1007/s00299-023-03057-y

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Citations (2)