Jinjun Shan

Nanjing University of Chinese Medicine

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Tong Xie

Nanjing University of Chinese Medicine

Liuqing Di

Nanjing University of Chinese Medicine

Wang Shou-chuan

Nanjing University of Chinese Medicine

Lili Lin

Fujian Agriculture and Forestry University

Jianya Xu

Sanya Central Hospital

Jianjian Ji

Nanjing University of Chinese Medicine

Cunsi Shen

Nanjing University of Chinese Medicine

An Kang

Northeast Agricultural University

Zichen Luo

Affiliated Hospital of North Sichuan Medical College

Wei Zhou

Chengdu University of Traditional Chinese Medicine

Cooperative Institutions

Nanjing University of Chinese Medicine

339

Jiangsu Province Hospital

Nanjing University

Nanjing Medical University

Chinese Academy of Medical Sciences & Peking Union Medical College

Chinese Academy of Sciences

Sichuan University

Capital Medical University

China Pharmaceutical University

Shanghai Jiao Tong University

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Effect of chito-oligosaccharide on the intestinal absorptions of phenylethanoid glycosides in Fructus Forsythiae extract

Phytomedicine (2014)

Wei Zhou Xiaobin Tan Jinjun Shan Ting Liu Baochang Cai

Phenylethanoid

Caco-2

Verbascoside

10.1016/j.phymed.2014.06.016

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Citations (28)

[Effect of respiratory syncytial virus-related pulmonary infection on endogenous metabolites in large intestinal mucosa in mice].

PubMed (2016)

Xin Meng Wang Shou-chuan Jinjun Shan Tong Xie Jianya Xu

To investigate the effect of respiratory syncytial virus (RSV)-related pulmonary infection on endogenous metabolites in large intestinal mucosa in BALB/c mice using metabolomics technology based on gas chromatography-mass spectrometry (GC-MS).Mice were randomly divided into a control group and a RSV pneumonia model group (n=16 each). The mouse model of RSV pneumonia was established using intranasal RSV infection (100×TCID50, 50 μL/mouse, once a day). After 7 days of intranasal RSV infection, the mice were sacrificed and GC-MS was used to identify endogenous metabolites and measure the changes in their relative content in colon tissue. SMCA-P12.0 software was used to perform principal component analysis (PCA) and orthogonal partial least squares-discriminant analysis (OPLS-DA) for endogenous metabolites in colon tissue. The differentially expressed metabolites in colon tissue were imported into the metabolic pathway platform Metaboanalyst to analyze related metabolic pathways.PCA and OPLS-DA showed significant differences between the control and RSV pneumonia model groups. A total of 32 metabolites were identified in the colon tissue of the mice with RSV pneumonia. The RSV pneumonia model group had significant increases in the content of leucine, isoleucine, glycine, alanine, arachidonic acid, and lactic acid, which were related to the valine, leucine, isoleucine, arachidonic acid, and pyruvic acid metabolic pathways.RSV pneumonia might cause metabolic disorders in the large intestinal tissue in mice.

Pyruvic acid

Isoleucine

Metabolic pathway

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Improvement of Intestinal Absorption of Forsythoside A and Chlorogenic Acid by Different Carboxymethyl Chitosan and Chito-oligosaccharide, Application to Flos Lonicerae - Fructus Forsythiae Herb Couple Preparations

PLoS ONE (2013)

Wei Zhou Haidan Wang Xuanxuan Zhu Jinjun Shan Ailing Yin

The current study aims to investigate the effect of chitosan derivatives on the intestinal absorption and bioavailabilities of forsythoside A (FTA) and Chlorogenic acid (CHA), the major active components in Flos Lonicerae - Fructus Forsythiae herb couple. Biopharmaceutics and pharmacokinetics properties of the two compounds have been characterized in vitro, in situ as well as in rats. Based on the identified biopharmaceutics characteristics of the two compounds, the effect of chitosan derivatives as an absorption enhancer on the intestinal absorption and pharmacokinetics of FTA and CHA in pure compound form as well as extract form were investigated in vitro, in situ and in vivo. Both FTA and CHA demonstrated very limited intestinal permeabilities, leading to oral bioavailabilities being only 0.50% and 0.13% in rats, respectively. Results from both in vitro, in situ as well as in vivo studies consistently indicated that Chito-oligosaccharide (COS) at dosage of 25 mg/kg could enhance intestinal permeabilities significantly as well as the in vivo bioavailabilities of both FTA and CHA than CMCs in Flos Lonicerae - Fructus Forsythiae herb couple preparations, and was safe for gastrointestine from morphological observation. Besides, treatment with Flos Lonicerae - Fructus Forsythiae herb couple preparations with COS at the dosage of 25 mg/kg prevented MDCK damage after influenza virus propagation, which was significantly better than control. The current findings not only identified the usefulness of COS for the improved delivery of Flos Lonicerae - Fructus Forsythiae preparations but also demonstrated the importance of biopharmaceutical characterization in the dosage form development of traditional Chinese medicine.

Flos

Chlorogenic Acid

10.1371/journal.pone.0063348

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Citations (28)

Discovery and Current Status of Evaluation System of Bioavailability and Related Pharmaceutical Technologies for Traditional Chinese Medicines—Flos Lonicerae Japonicae—Fructus Forsythiae Herb Couples as an Example

International Journal of Molecular Sciences (2015)

Wei Zhou Baochang Cai Jinjun Shan Wang Shou-chuan Liuqing Di

Traditional Chinese medicines (TCMs) have attracted extensive interest throughout the world due to their long history of health protection and disease control, and the internalization of TCM preparations or patented drugs has been considered a wind vane in the process of TCM modernization. However, multi-target effects, caused by multiple components in TCMs, hinder not only the construction of the quality evaluation system (bioavailability), but also the application of pharmaceutical technologies, which results in the poor efficacy in clinical practice. This review describes the methods in the literature as well as in our thoughts about how to identify the marker components, establish the evaluation system of bioavailability, and improve the bioavailability in TCM preparations. We expect that the current study will be positive and informative.

Flos

10.3390/ijms161226132

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Citations (20)

Size-dependent effects of polystyrene microplastics on gut metagenome and antibiotic resistance in C57BL/6 mice

Ecotoxicology and Environmental Safety (2023)

Bei Gao Xiaochun Shi Shanshan Li Weichen Xu Nan Gao

Microplastic pollution is an emerging threat for marine and terrestrial ecosystems, which has raised global concerns about its implications for human health. Mounting evidence has shown that the gut microbiota plays a key role in human health and diseases. The gut bacteria could be disturbed by many environmental factors, including the microplastic particles. However, the size effect of polystyrene microplastics on mycobiome, as well as gut functional metagenome has not been well studied. In this study, we performed ITS sequencing to explore the size effect of polystyrene microplastics on the fungal composition, in combination with the shotgun metagenomics sequencing to reveal the size effects of polystyrene on the functional metagenome. We found that polystyrene microplastic particles with 0.05-0.1 µm diameter showed greater impact on the bacterial and fungal composition of gut microbiota as well as the metabolic pathways than the polystyrene microplastic particles with 9-10 µm diameter. Our results suggested that size-depended effects should not be ignored in the health risk assessment of microplastics.

Polystyrene

Expanded polystyrene

10.1016/j.ecoenv.2023.114737

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Network pharmacology combined with lipidomics to reveal the regulatory effects and mechanisms of Kangzao granules in the hypothalamus of rats with central precocious puberty

Journal of Pharmaceutical and Biomedical Analysis (2024)

Yanhua Jiang Lixia Yang Hui Chen Jiabin Chen Lingling Yang

Orbitrap

KEGG

Docking (animal)

10.1016/j.jpba.2024.116059

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Development and application of a comprehensive lipidomic analysis to investigate Tripterygium wilfordii-induced liver injury

Analytical and Bioanalytical Chemistry (2016)

Tong Xie Xueping Zhou Wang Shou-chuan Yan Lu Huaxu Zhu

Lipidome

Metabolic pathway

10.1007/s00216-016-9533-9

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Citations (25)

Glycyrrhizic acid improving the liver protective effect by restoring the composition of Lactobacillus

Journal of Functional Foods (2018)

Tianjie Yuan Jing Wang Letian Chen Jinjun Shan Liuqing Di

Dysbiosis

Microecology

CCL4

10.1016/j.jff.2018.11.001

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Citations (11)

Elevated monocytic myeloid‐derived suppressor cells positively correlate with infection frequency in children with RRTIs

European Journal of Immunology (2020)

Jianjian Ji Lishun Ren Yuling Liu Min Zheng Huazhong Fang

In children with recurrent respiratory tract infections (RRTIs), the percentages and numbers of monocytic myeloid-derived suppressor cells (MDSCs) were elevated. The elevated MDSCs positively correlate with respiratory tract infection frequency in RRTIs children. The elevated MDSCs can inhibit CD8+ T-cells proliferation in RRTIs children.

Myeloid-derived Suppressor Cell

10.1002/eji.202048629

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Citations (2)

An LC-MS/MS Method for the Pharmacokinetic and In Vitro Metabolism Studies of Praeruptorin A in Rat

Current Pharmaceutical Analysis (2021)

An Kang Zhuicheng Xu Jinjun Shan Mengmeng Song Tong Xie

Objective: The study aims to investigate the pharmacokinetic profile of Praeruptorin A and khellactone and in vitro hydrolysis of praeruptorin A to khellactone in different biological samples. Methods: A LC-MS/MS method was established. Analytes and internal standard (IS) were isolated using the protein precipitation method and then separated on a Thermo BDS Hypersil C18 (2.1 mm×50 mm, 2.4μm) column using a mobile phase consisting of 0.05% formic acid solution and acetonitrile. Samples were analyzed in positive electrospray-ionization (ESI) mode using multiple reaction monitoring (MRM). Results: The calibration plots gave desirable linearity (r2>0.99) in the concentration range from 0.99-990.0 and 2.0-2000.0 ng/mL for Praeruptorin A and khellactone, respectively. In addition, the LOQs of these analytes were sufficient for vivo pharmacokinetic study and vitro hydrolysis study of Praeruptorin A. The intra-batch and inter-batch precision were all within 14.05%, and the accuracy was between 89.39% and 109.50%. The extraction efficiency of PA and khellactone ranged from 76.35 ~ 89.58%. The matrix effects of analytes and the IS were between 89.67% ~ 105.26%. Conclusion: The liver CYPs mediated by the metabolism of PA may contribute to the systemic exposure of its active metabolite, khellactone, in rats.

Protein precipitation

Sample Preparation

10.2174/1573412917666210827103645

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