喉頭瘢痕症例では, ときに病態が複雑で治療に難渋することがある. 内視鏡では判断できない深部構造の異常が声帯粘膜波動や声帯運動の障害の原因となっている可能性がある. したがって声帯病変のみならず深部の瘢痕, 喉頭の枠組みの変形, 披裂軟骨の偏位や運動障害の有無についても把握する必要がある. 声帯内脂肪注入例, 喉頭外傷症例および喉頭外傷後に喉頭截開術を受けた症例を例に挙げ, 深部に存在する構造異常が声帯の粘膜波動や運動に及ぼす影響について3DCTで検討した. これらの症例は声帯の粘膜波動や声帯運動の障害を認め, 瘢痕病変の存在が疑われた症例である. 声帯内脂肪注入例では過注入により両側の声帯が緊満した結果, 声帯の粘膜波動が障害された. 喉頭外傷症例では輪状披裂関節部での輪状軟骨の骨折と披裂軟骨の脱臼を認めた. 喉頭截開術後の症例では, 片側の披裂軟骨脱臼および両側の甲状軟骨板の上下方向のずれによる声帯レベルの差を認めた. これらは声帯粘膜波動や声帯運動の障害の原因であった. 喉頭瘢痕による音声障害の治療において, 内視鏡で観察できる部分だけでなく, 深部に存在する構造異常の有無についても考慮する必要がある. 3DCTは喉頭領域の複雑な立体配置の描出が可能であり, 喉頭瘢痕症例の評価に適している.

10.5112/jjlp.51.171

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Several Factors Contribute to Dietary Content of Trace Elements

Seikatsu eisei (2007)

Kaoru Yoshida Makiko Umezawa Mari Kitamura Koichi Kuroda

TRACE (psycholinguistics)

Content (measure theory)

10.11468/seikatsueisei.51.385

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The antidiabetic drug metformin inhibits uterine leiomyoma cell proliferation via an AMP-activated protein kinase signaling pathway

Gynecological Endocrinology (2012)

Bin Li Takashi Takeda Kenji Tsuiji Akiko Kondo Mari Kitamura

Uterine leiomyomas are the most common gynecological benign tumors and greatly affect reproductive health and wellbeing. Metformin is the most widely used antidiabetic drug in the world, and there is increasing evidence of a potential efficacy of this agent as an anticancer drug. In order to understand metformin's anti-tumorigenic potential better, in this study, we investigated the inhibitory effect of metformin and expression of key targets of metformin cell signaling in leiomyoma cells. Cell proliferation was assessed after exposure to metformin. Apoptosis was assessed by western blotting for cleaved-PARP and TUNEL staining. The expressions of phosphorylated AMPK and phosphorylated S6 were determined by western blotting. Metformin potently inhibited ELT-3 cell proliferation in a dose-dependent manner. Western blotting analysis demonstrated that metformin induced phosphorylation of AMPK and the inhibitory effect was attenuated with AMPK inhibitor, compound C. In parallel, treatment with metformin decreased phosphorylation of S6 protein. These experimental findings show that metformin is a potent inhibitor of cell proliferation in leiomyoma cells. This effect is mediated by AMPK activation and subsequent inhibition of the mTOR pathway. Thus, this study provides a possible mechanism of the action of metformin in the inhibition of leiomyoma cell growth.

Uterine Leiomyoma

10.3109/09513590.2012.706668

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IL-12 and IL-18 Induction and Subsequent NKT Activation Effects of the Japanese Botanical Medicine Juzentaihoto

International Journal of Molecular Sciences (2008)

Kazuhiko Fujiki Masanori Nakamura Takako Matsuda Mariko Isogai Minako Ikeda

In this study, we first measured some cytokine concentrations in the serum of patients treated with Juzentaihoto (JTT). Of the cytokines measured interleukin (IL) -18 was the most prominently up-regulated cytokine in the serum of patients under long term JTT administration. We next evaluated the effects of JTT in mice, focusing especially on natural killer T (NKT) cell induction. Mice fed JTT were compared to control group ones. After sacrifice, the liver was fixed, embedded and stained. Transmission electron microscope (TEM) observations were performed. Although the mice receiving the herbal medicine had same appearance, their livers were infiltrated with massive mononuclear cells, some of which were aggregated to form clusters. Immunohistochemical staining revealed that there was abundant cytokine expression of IL-12 and IL-18 in the liver of JTT treated mice. To clarify what the key molecules that induce immunological restoration with JTT might be, we next examined in vitro lymphocyte cultures. Mononuclear cells isolated and prepared from healthy volunteers were cultured with and without JTT. Within 24 hours, JTT induced the IL-12 and IL-18 production and later (72 hours) induction of interferon (IFN)-gamma. Oral administration of JTT may induce the expression of IL-12 in the early stage, and IL-18 in the chronic stage, followed by NKT induction. Their activation, following immunological restoration could contribute to anti-tumor effects.

10.3390/ijms9071142

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Citations (25)

Difference between Utilization of Print and Online Versions of Dietary Education Materials for Preschoolers’ Families

The Japanese Journal of Nutrition and Dietetics (2022)

Mari Kitamura Hiroko Nakanishi Rie Horiuchi

【目的】保育所から配付された印刷型，オンライン型の食育教材の家庭での活用状況を調べ，閲覧，活用に繋がる提供方法を検討する。

10.5264/eiyogakuzashi.80.265

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Cysteine enhances clastogenic activity of dimethylarsinic acid

Applied Organometallic Chemistry (2002)

Mari Kitamura Koichi Kuroda Yoko Endo Ginji Endo

Abstract The effects of cysteine on dimethylarsinic acid (DMA)‐induced cytotoxicity and chromosomal aberration were studied using Chinese hamster V79 cells. The IC 50 of DMA, i.e. the concentration resulting in a 50% decrease in cell population of viable cells, was 130 µg ml −1 (0.94 m M ), whereas that in the presence of 50 µg ml −1 (0.28 m M ) cysteine was 20 µg ml −1 (0.14 m M ). The mitotic index with co‐administration of 50 µg ml −1 (0.36 m M ) DMA and 50 µg ml −1 cysteine was 1.4 times that with 50 µg ml −1 DMA alone. Whereas 82% of cells divided twice with 25 µg ml −1 (0.18 m M ) DMA alone, most cells divided only once with co‐administration of 25 µg ml −1 DMA and 50 µg ml −1 cysteine. These results indicated that the increase in mitotic index by cysteine was due to enhancement of mitotic arrest by DMA. With co‐administration of 25 µg ml −1 DMA and 50 µg ml −1 cysteine, tetraploidy was 14.3% higher and fivefold by that with 25 µg ml −1 DMA only. Cysteine at 50 µg ml −1 enhanced induction of chromosomal aberrant cells by DMA. 100 µg ml −1 (0.72 m M ) DMA induced 91% chromosomal aberrant cells in the presence of cysteine, and 12% in the absence of cysteine. Chromatid breaks and chromatid gaps were the most frequent types of aberration induced by co‐administration of DMA and cysteine or DMA alone. Co‐administration of DMA and cysteine produced many attenuated chromosomal figures. The attenuated chromosomal figures always had several chromatid gaps and chromatid breaks. Our findings may provide clues to arsenic carcinogenesis in humans. Copyright © 2002 John Wiley & Sons, Ltd.

Clastogen

Mitotic index

10.1002/aoc.310

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The possibility of excessive intake of trace elements by taking supplements: questionnaire survey on use and perception of dietary supplement

Kaoru Yoshida Maki Kiriki Yuko Kubota Yui Joko Mari Kitamura

Summary Trace elements, such as zinc (Zn), iron (Fe), cupper (Cu) and manganese (Mn) are essential elements but excess amounts of these elements are known to cause many neurodegenerative diseases including Alzheimer's disease and Parkinson's disease. Some supplements of botanical or animal origin contained high concentrations of Zn, Fe, Cu, Mn and aluminium (Al). The risk of excessive intake of Zn, Fe, Cu, Mn and Al is increased by trace elements in food and in dietary supplements. We conducted a questionnaire survey to investigate the use of dietary supplements and dietary habits in 322 young, middle-aged, and older adults. 44.2% of men and 60.6% of women subjects were using or had used supple- ments. The half of women over 50 years old was using supplements. Supplement users had more healthy eating habits than the non-users. 73.2% of the dietary supplement users took the supplements every day. Many current us- ers were taking supplements of botanical or animal origin. These results suggest that the risk of excessive intake of the trace elements is caused by taking food plus botanical or animal origin supplements.

Dietary Reference Intake

Dietary supplement

Food habits

Food supplement

Source

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The Japanese Journal of Nutrition and Dietetics (2002)

Mari Kitamura Sachiko Horiuchi Sadanobu Tsukada M. Fujiwara Mikio Okamura

10.5264/eiyogakuzashi.60.25

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