Agnieszka Włodarczyk

Medical University of Silesia

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Magdalena Rost–Roszkowska

University of Silesia in Katowice

L Sonakowska

University of Silesia in Katowice

K Kamińska

University of Silesia in Katowice

Sebastian Student

Silesian University of Technology

Marta Hyra

University of Silesia in Katowice

P. Włodarczyk

Łukasiewicz Research Network - Institute of Non-Ferrous Metals

Izabela Poprawa

University of Silesia in Katowice

Renata Matlakowska

University of Warsaw

A. Dobek

Adam Mickiewicz University in Poznań

A. Patkowski

Adam Mickiewicz University in Poznań

Cooperative Institutions

University of Silesia in Katowice

Institute of Physics

Silesian University of Technology

University of Warsaw

Silesian Center for Heart Disease

Polish Academy of Sciences

Medical University of Silesia

Silesian University in Opava

Adam Mickiewicz University in Poznań

Czech Academy of Sciences, Institute of Physics

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Effect of Ions on the Polymorphism, Effective Charge, and Stability of Human Telomeric DNA. Photon Correlation Spectroscopy and Circular Dichroism Studies

The Journal of Physical Chemistry B (2005)

Agnieszka Włodarczyk Przemysław R. Grzybowski A. Patkowski A. Dobek

The effect of different ions on the formation and behavior of quadruplex structures of the human telomere sequence d(TTAGGG)4 has been studied by photon correlation spectroscopy (PCS) and circular dichroism (CD). The saturation and melting curves obtained in the presence of K+, Na+, Rb+, Li+, Cs+, and Sr2+ ions were recorded by CD spectroscopy and indicated the formation of monomeric quadruplexes. Analysis of the saturation curves obtained at 2 °C has shown that the presence of a single Sr2+ ion per oligomer is sufficient for the formation of a monomeric quadruplex of the DNA sequence studied. In the presence of SrCl2 at a concentration of 50 mM, the formation of tetrameric quadruplexes has been detected. The effect of Sr2+ ions on the formation of quadruplex structures by the human telomere sequence d(TTAGGG)4 is stronger and different from that of the other ions tested. The paper also presents results of a study of electrostatic interactions in solution. The translation diffusion coefficients DT of the structures present in solution have been determined by photon correlation spectroscopy and the effective charges on the structures have been calculated by combining the experimental data with the results based on the coupled mode theory. Analysis of the melting points monitored by the CD method has permitted a determination of Δn, the number of ions released in the process of thermal denaturation. All the results are in good agreement with the predictions based on the theory of polyelectrolytes. The effect of ions on the formation and behavior of quadruplex structures of the human telomere sequence d(TTAGGG)4 has been studied by photon correlation spectroscopy and circular dichroism.

10.1021/jp045274d

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Citations (100)

Autophagy and apoptosis in starved and refedNeocaridina davidi(Crustacea, Malacostraca) midgut

Canadian Journal of Zoology (2018)

Agnieszka Włodarczyk Sebastian Student Magdalena Rost‐Roszkowska

Adult specimens of the freshwater shrimp Neocaridina davidi Bouvier, 1904 (Crustacea) were starved for 7, 14, and 21 days. Specimens from the first and second experimental group were collected for the studies. The majority of animals starved for 21 days died. Additionally, some specimens from each group were refed for 4, 7, and 14 days. The epithelium of the midgut, which is composed of the intestine and hepatopancreas, was analyzed. While the epithelium of the intestine is formed by D- and R-cells, the epithelium of the hepatopancreas has R-, B-, and F-cells. Autophagy and apoptosis in the midgut epithelium were analyzed using transmission electron microscopy and immunohistochemical methods. These processes were only observed in the D-cells of the intestine and the F- and B-cells of the hepatopancreas. Starvation led to a reduction in the amount of reserve material in the B-cells. Although this process activated autophagy in both regions of the midgut, the intestine and hepatopancreas, after refeeding, the level of autophagy decreased. Starvation caused an increase in the apoptotic cells in both organs, while the refeeding caused a decrease in the number of apoptotic cells in both organs analyzed. Refeeding after periods of starvation caused an accumulation of reserve material in the hepatopancreas.

Hepatopancreas

Malacostraca

10.1139/cjz-2018-0104

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Citations (16)

Ultrastructure of the salivary glands in Lithobius forficatus (Myriapoda, Chilopoda, Lithobiidae) according to seasonal and circadian rhythms

Arthropod Structure & Development (2016)

K Kamińska Agnieszka Włodarczyk L Sonakowska A. Ostróżka A. Marchewka

Myriapoda

10.1016/j.asd.2016.09.007

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Citations (9)

The Oxidative Metabolism of Fossil Hydrocarbons and Sulfide Minerals by the Lithobiontic Microbial Community Inhabiting Deep Subterrestrial Kupferschiefer Black Shale

Frontiers in Microbiology (2018)

Agnieszka Włodarczyk Maciej Lirski Anna Fogtman Marta Koblowska Grzegorz Bidziński

Black shales are one of the largest reservoirs of fossil organic carbon and inorganic reduced sulfur on Earth. It is assumed that microorganisms play an important role in the transformations of these sedimentary rocks and contribute to the return of organic carbon and inorganic sulfur to the global geochemical cycles. An outcrop of deep subterrestrial ~256-million-year-old Kupferschiefer black shale was studied to define the metabolic processes of the deep biosphere important in transformations of organic carbon and inorganic reduced sulfur compounds. This outcrop was created during mining activity 12 years ago and since then it has been exposed to the activity of oxygen and microorganisms. The microbial processes were described based on metagenome and metaproteome studies as well as on the geochemistry of the rock. The microorganisms inhabiting the subterrestrial black shale were dominated by bacterial genera such as Pseudomonas, Limnobacter, Yonghaparkia, Thiobacillus, Bradyrhizobium, and Sulfuricaulis. This study on black shale was the first to detect archaea and fungi, represented by Nitrososphaera and Aspergillus genera, respectively. The enzymatic oxidation of fossil aliphatic and aromatic hydrocarbons was mediated mostly by chemoorganotrophic bacteria, but also by archaea and fungi. The dissimilative enzymatic oxidation of primary reduced sulfur compounds was performed by chemolithotrophic bacteria. The geochemical consequences of microbial activity were the oxidation and dehydrogenation of kerogen, as well as oxidation of sulfide minerals.

Thiobacillus

10.3389/fmicb.2018.00972

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Citations (19)

Voltage sensitive dye imaging of transient neuronal assemblies in brain slices under hyperbaric conditions.

Undersea and Hyperbaric Medicine (2008)

Agnieszka Włodarczyk PF McMillan S A Greenfield

We have developed a high pressure optical cell to study large-scale transient neuronal coalitions--"assemblies" using voltage sensitive dye (VSD) fluorescence combined with fast CCD imaging of brain slices under hyperbaric conditions. The new cell has been tested at pressures up to P = 150-200 atm, corresponding to the range over which effects such as "pressure-reversal" of anaesthesia have been described previously. Brain slices were maintained in a flow of artificial cerebrospinal fluid (aCSF) and hyperbaric conditions were controlled to within +/- 0.2 atm using a back-pressure regulator placed in the pumping system. Preliminary VSD imaging experiments were carried out on rat hippocampal slices at pressures up to P approximately 50 atm. An electrode placed in the CA3 region was used to stimulate a signal along the Schaffer collateral towards CA1. First results indicate that good VSD data can be obtained that can be analysed to provide a new view on how hydrostatic pressurisation affects the dynamic propagation of neuronal assemblies.

Hydrostatic pressure

Transient (computer programming)

SIGNAL (programming language)

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Citations (2)

Influence of the modifiers in polyol method on magnetically induced hyperthermia and biocompatibility of ultrafine magnetite nanoparticles

Scientific Reports (2023)

Adrian Radoń Agnieszka Włodarczyk Łukasz Sieroń Magdalena Rost‐Roszkowska Łukasz Chajec

Magnetite nanoparticles (Fe3O4 NPs) are widely tested in various biomedical applications, including magnetically induced hyperthermia. In this study, the influence of the modifiers, i.e., urotropine, polyethylene glycol, and NH4HCO3, on the size, morphology, magnetically induced hyperthermia effect, and biocompatibility were tested for Fe3O4 NPs synthesized by polyol method. The nanoparticles were characterized by a spherical shape and similar size of around 10 nm. At the same time, their surface is functionalized by triethylene glycol or polyethylene glycol, depending on the modifiers. The Fe3O4 NPs synthesized in the presence of urotropine had the highest colloidal stability related to the high positive value of zeta potential (26.03 ± 0.55 mV) but were characterized by the lowest specific absorption rate (SAR) and intrinsic loss power (ILP). The highest potential in the hyperthermia applications have NPs synthesized using NH4HCO3, for which SAR and ILP were equal to 69.6 ± 5.2 W/g and 0.613 ± 0.051 nHm2/kg, respectively. Their application possibility was confirmed for a wide range of magnetic fields and by cytotoxicity tests. The absence of differences in toxicity to dermal fibroblasts between all studied NPs was confirmed. Additionally, no significant changes in the ultrastructure of fibroblast cells were observed apart from the gradual increase in the number of autophagous structures.

Biocompatibility

Triethylene glycol

Zeta potential

Magnetic hyperthermia

Superparamagnetism

10.1038/s41598-023-34738-z

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Citations (18)

Fine structure and histochemical studies on salivary glands and fat body in centipedes (Myriapoda, Chilopoda)

Acta Biologica Cracoviensia. Series Zoologia (2016)

K Kamińska Agnieszka Włodarczyk L Sonakowska Magdalena Rost–Roszkowska

Myriapoda

Centipede

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A novel approach for studying 3D embryo development of crustaceans (freshwater shrimp Neocaridina heteropoda) using the X-ray Microtomography

L Sonakowska Joanna Śróbka Katarzyna Janiszewska K Kamińska Agnieszka Włodarczyk

X-ray microtomography

Freshwater shrimp

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Structure and Ultrastructure of the Endodermal Region of the Alimentary Tract in the Freshwater Shrimp Neocaridina heteropoda (Crustacea, Malacostraca)

PLoS ONE (2015)

L Sonakowska Agnieszka Włodarczyk Izabela Poprawa Marcin Binkowski Joanna Śróbka

The freshwater shrimp Neocaridina heteropoda (Crustacea, Malacostraca, Decapoda) originates from Asia and is one of the species that is widely available all over the world because it is the most popular shrimp that is bred in aquaria. The structure and the ultrastructure of the midgut have been described using X-ray microtomography, transmission electron microscopy, light and fluorescence microscopes. The endodermal region of the alimentary system in N. heteropoda consists of an intestine and a hepatopancreas. No differences were observed in the structure and ultrastructure of males and females of the shrimp that were examined. The intestine is a tube-shaped organ and the hepatopancreas is composed of two large diverticles that are divided into the blind-end tubules. Hepatopancreatic tubules have three distinct zones - proximal, medial and distal. Among the epithelial cells of the intestine, two types of cells were distinguished - D and E-cells, while three types of cells were observed in the epithelium of the hepatopancreas - F, B and E-cells. Our studies showed that the regionalization in the activity of cells occurs along the length of the hepatopancreatic tubules. The role and ultrastructure of all types of epithelial cells are discussed, with the special emphasis on the function of the E-cells, which are the midgut regenerative cells. Additionally, we present the first report on the existence of an intercellular junction that is connected with the E-cells of Crustacea.

Hepatopancreas

Malacostraca

Penaeus monodon

10.1371/journal.pone.0126900

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Citations (37)

Relationship between ROS production, MnSOD activation and periods of fasting and re-feeding in freshwater shrimpNeocaridina davidi(Crustacea, Malacostraca)

PeerJ (2019)

Agnieszka Włodarczyk Grażyna Wilczek Piotr Wilczek Sebastian Student A. Ostróżka

The middle region of the digestive system, the midgut of freshwater shrimp Neocaridina davidi is composed of a tube-shaped intestine and the hepatopancreas formed by numerous caeca. Two types of cells have been distinguished in the intestine, the digestive cells (D-cells) and regenerative cells (R-cells). The hepatopancreatic tubules have three distinct zones distinguished along the length of each tubule-the distal zone with R-cells, the medial zone with differentiating cells, and the proximal zone with F-cells (fibrillar cells) and B-cells (storage cells). Fasting causes activation of cell death, a reduction in the amount of reserve material, and changes in the mitochondrial membrane potential. However, here we present how the concentration of ROS changes according to different periods of fasting and whether re-feeding causes their decrease. In addition, the activation/deactivation of mitochondrial superoxide dismutase (MnSOD) was analyzed. The freshwater shrimps Neocaridina davidi (Crustacea, Malacostraca, Decapoda) were divided into experimental groups: animals starved for 14 days, animals re-fed for 4, 7, and 14 days. The material was examined using the confocal microscope and the flow cytometry. Our studies have shown that long-term starvation increases the concentration of free radicals and MnSOD concentration in the intestine and hepatopancreas, while return to feeding causes their decrease in both organs examined. Therefore, we concluded that a distinct relationship between MnSOD concentration, ROS activation, cell death activation and changes in the mitochondrial membrane potential occurred.

Malacostraca

Hepatopancreas

10.7717/peerj.7399

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Citations (22)