Wonmok Lee

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

CTX-M-55-Type Extended-Spectrum β-lactamase- Producing Shigella sonnei Isolated from a Korean Patient Who Had Travelled to China

Annals of Laboratory Medicine (2013)

Wonmok Lee Hae‐Sun Chung Hyukmin Lee Jong Hwa Yum Dongeun Yong

Wonmok Lee, M.D., Hae-Sun Chung, M.D., Hyukmin Lee, M.D., Jong Hwa Yum, Ph.D., Dongeun Yong, M.D., Seok Hoon Jeong, M.D., Kyungwon Lee, M.D., and Yunsop Chong, Ph.D.. Ann Lab Med 2013;33:141-4. https://doi.org/10.3343/alm.2013.33.2.141

Shigella sonnei

10.3343/alm.2013.33.2.141

Cite

Citations (28)

Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria

Annals of Laboratory Medicine (2014)

Wonmok Lee Myung-Sook Kim Dongeun Yong Seok Hoon Jeong Kyungwon Lee

By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMérieux, France) in the identification of anaerobes.We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing.Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level.The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria.

10.3343/alm.2015.35.1.69

Cite

Citations (39)

BRCA1/2 mutations, including large genomic rearrangements, among unselected ovarian cancer patients in Korea

Journal of Gynecologic Oncology (2018)

Do‐Hoon Kim Chi‐Heum Cho Sun Young Kwon Nam Hee Ryoo Dong‐Seok Jeon

Objective: We performed small-scale mutation and large genomic rearrangement (LGR) analysis of BRCA1/2 in ovarian cancer patients to determine the prevalence and the characteristics of the mutations.Methods: All ovarian cancer patients who visited a single institution between September 2015 and April 2017 were included.Sanger sequencing, multiplex ligation-dependent probe amplification (MLPA), and long-range polymerase chain reaction (PCR) were performed to comprehensively study BRCA1/2.The genetic risk models BRCAPRO, Myriad, and BOADICEA were used to evaluate the mutation analysis.Results: In total, 131 patients were enrolled.Of the 131 patients, Sanger sequencing identified 16 different BRCA1/2 small-scale mutations in 20 patients (15.3%).Two novel nonsense mutations were detected in 2 patients with a serous borderline tumor and a large-cell neuroendocrine carcinoma.MLPA analysis of BRCA1/2 in Sanger-negative patients revealed 2 LGRs.The LGRs accounted for 14.3% of all identified BRCA1 mutations, and the prevalence of LGRs identified in this study was 1.8% in 111 Sanger-negative patients.The genetic risk models showed statistically significant differences between mutation carriers and non-carriers.The 2 patients with LGRs had at least one blood relative with breast or ovarian cancer.Conclusion: Twenty-two (16.8%) of the unselected ovarian cancer patients had BRCA1/2 mutations that were detected through comprehensive BRCA1/2 genetic testing.Ovarian cancer patients with Sanger-negative results should be considered for LGR detection if they have one blood relative with breast or ovarian cancer.The detection of more BRCA1/2 mutations in patients is important for efforts to provide targeted therapy to ovarian cancer patients.

Sanger sequencing

Nonsense mutation

10.3802/jgo.2018.29.e90

Cite

Citations (8)

Correlation Between C3d Assay and Single Antigen Bead Assay for Detection of Human Leukocyte Antigen Class II Antibodies

Laboratory Medicine Online (2020)

H S Kang Do‐Hoon Kim Jung‐Sook Ha Nam Hee Ryoo Dong‐Seok Jeon

의해 HLA 항체가 생성될 수 있고, 이는 고형 장기이식 수혜자에게 항체매개성 거부반응(antibody-mediated rejection)을 유발할 수 있다[1, 2].HLA는 그 종류가 매우 다양하므로 정확한 HLA 항체의 검출,

Bead

10.47429/lmo.2020.10.4.295

Cite

Citations (0)

Establishing Quality Control Ranges for Antimicrobial Susceptibility Testing of Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus: A Cornerstone to Develop Reference Strains for Korean Clinical Microbiology Laboratories

Annals of Laboratory Medicine (2015)

Sung Kuk Hong Seung Jun Choi Saeam Shin Wonmok Lee Naina Adren Pinto

Quality control (QC) processes are being performed in the majority of clinical microbiology laboratories to ensure the performance of microbial identification and antimicrobial susceptibility testing by using ATCC strains. To obtain these ATCC strains, some inconveniences are encountered concerning the purchase cost of the strains and the shipping time required. This study was focused on constructing a database of reference strains for QC processes using domestic bacterial strains, concentrating primarily on antimicrobial susceptibility testing. Three strains (Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus) that showed legible results in preliminary testing were selected. The minimal inhibitory concentrations (MICs) and zone diameters (ZDs) of eight antimicrobials for each strain were determined according to the CLSI M23. All resulting MIC and ZD ranges included at least 95% of the data. The ZD QC ranges obtained by using the CLSI method were less than 12 mm, and the MIC QC ranges extended no more than five dilutions. This study is a preliminary attempt to construct a bank of Korean QC strains. With further studies, a positive outcome toward cost and time reduction can be anticipated.

Clinical Microbiology

Serial dilution

10.3343/alm.2015.35.6.635

Cite

Citations (5)

A Case of Atypical CML with Micro BCR/ABL Rearrangement

The Korean Journal of Hematology (2008)

Jihye Kim Wonmok Lee Nam Hee Ryoo Jung‐Sook Ha Dong‐Seok Jeon

Microtype BCR/ABL rearrangement is extremely rare and has been known to be associated with neutrophilic chronic myeloid leukemia (N-CML). However, there is more to be understood regarding this phenotype. We report a case of atypical CML that exhibited micro BCR/ABL rearrangement with predominant thrombocytosis. Our patient showed thrombocytosis (1,464×109/L) and megakaryocytosis in the peripheral blood and bone marrow. However, neither leukocytosis nor neutrophilia was observed (white blood cells (WBC), 5.02×109/L neutrophils, 45%). Bone marrow aspirate revealed increased cellularity: 12% basophils, 6% eosinophils, and 9% blasts. The 46,XX,t(9;22)(q34;q11.2),i(17q) chromosome complement was observed in 4 of 20 metaphase cells, and standard BCR/ABL fusion signals were observed in 10% of interphase cells on fluorescence in situ-hybridization (FISH) analysis. Reverse transcriptase-polymerase chain reaction (RT-PCR) was used to acquire the BCR/ABL fusion transcript, the identity of which was confirmed via sequence analysis. Hematologic remission was achieved 2 months after imatinib therapy initiation, and molecular remission was achieved 2 months after hematologic remission. The patient is currently undergoing regular follow-up visits and is in good health. However, further long-term follow up is warranted. The incorporation of imatinib into therapeutic strategies may be further established through the study of more cases of micro BCR/ABL.

Thrombocytosis

Leukocytosis

breakpoint cluster region

Neutrophilia

Imatinib Mesylate

ABL

Philadelphia chromosome

10.5045/kjh.2008.43.3.184

Cite

Citations (1)

Comparison and Evaluation of Diagnostic Assays forClostridium difficileInfection

Laboratory Medicine Online (2017)