Jessica Bispo

Universidade de São Paulo

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Fernanda Esposito

Universidade de São Paulo

Nilton Lincopán

Universidade de São Paulo

Bruna Fuga

Universidade de São Paulo

Herrison Fontana

Universidade de São Paulo

Flávio A. Oliveira

Universidade Estadual de Campinas (UNICAMP)

P.P. Power

University of Buenos Aires

Brenda Cardoso

Universidade de São Paulo

Elder Sano

Universidade de São Paulo

Gabriel Gutkind

University of Buenos Aires

Susan Ienne

Universidade de São Paulo

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Universidade de São Paulo

Institute of Biomedical Science

Hospital Universitário da Universidade de São Paulo

University of Buenos Aires

Consejo Nacional de Investigaciones Científicas y Técnicas

Monash University

Universidade Estadual de Campinas (UNICAMP)

Hospital de Clínicas da Unicamp

Universidade Federal de Minas Gerais

Instituto Butantan

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Critical priority third-generation cephalosporin-resistant Escherichia coli in antibiotic- free retail meat

Research Square (Research Square) (2024)

Bruna Fuga Ingrith Neves Herrison Fontana Jessica Bispo Elder Sano

Abstract Contamination of food by critical priority third-generation cephalosporin-resistant Escherichia coli poses a significant risk to consumers, potentially leading to foodborne illnesses, silent colonization, and/or antibiotic resistance dissemination. In this study, we report the microbiological and genomic features of ESβL/pAmpC-producing E. coli in conventional (CN) and antibiotic-free (AF) retail meat samples sold in different markets in São Paulo city. Nine representative chicken isolates were investigated by whole-genome sequencing. E. coli ST38 (CTX-M-2), ST350 (CMY-2), ST2179 (CTX-M-8), and ST2040 (CTX-M-55) clones were identified in meat samples from CN, and E. coli clones ST57 (CMY-2), ST117 (CTX-M-55), ST443 (CTX-M-2), ST15579 (CTX-M-8), and ST3258 (CTX-M-55) from AF farming systems. Resistomes to other clinically relevant antibiotics, hazardous heavy metals, disinfectants, and herbicides, were also predicted; whereas a broad virulome was detected in E. coli ST2040 and ST350. High-risk lineages ST38 and ST117 were identified in frozen meat from CN and AF, respectively, whereas phylogenetic analysis revealed relationship with poultry clones previously identified in the United States of America and Brazil. Our findings add new perspectives to current epidemiological knowledge regarding the occurrence of ESβL/pAmpC-producing E. coli in the meat industry, reinforcing the need for genomic surveillance of WHO critical priority pathogens in different rearing systems.

Cephalosporin Antibiotic

10.21203/rs.3.rs-4823812/v1

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Emergence of KPC-113 and KPC-114 variants in ceftazidime-avibactam-resistant Klebsiella pneumoniae belonging to high-risk clones ST11 and ST16 in South America

Microbiology Spectrum (2023)

Felipe Vásquez-Ponce Jessica Bispo Johana Becerra Herrison Fontana Jesus G. M. Pariona

Two novel variants of Klebsiella pneumoniae carbapenemase (KPC) associated with resistance to ceftazidime-avibactam (CZA) and designated as KPC-113 and KPC-114 by NCBI were identified in 2020, in clinical isolates of Klebsiella pneumoniae in Brazil. While K. pneumoniae of ST16 harbored the blaKPC-113 variant on an IncFII-IncFIB plasmid, K. pneumoniae of ST11 carried the blaKPC-114 variant on an IncN plasmid. Both isolates displayed resistance to broad-spectrum cephalosporins, β-lactam inhibitors, and ertapenem and doripenem, whereas K. pneumoniae producing KPC-114 showed susceptibility to imipenem and meropenem. Whole-genome sequencing and in silico analysis revealed that KPC-113 presented a Gly insertion between Ambler positions 264 and 265 (R264_A265insG), whereas KPC-114 displayed two amino acid insertions (Ser-Ser) between Ambler positions 181 and 182 (S181_P182insSS) in KPC-2, responsible for CZA resistance profiles. Our results confirm the emergence of novel KPC variants associated with resistance to CZA in international clones of K. pneumoniae circulating in South America. IMPORTANCE KPC-2 carbapenemases are endemic in Latin America. In this regard, in 2018, ceftazidime-avibactam (CZA) was authorized for clinical use in Brazil due to its significant activity against KPC-2 producers. In recent years, reports of resistance to CZA have increased in this country, limiting its clinical application. In this study, we report the emergence of two novel KPC-2 variants, named KPC-113 and KPC-114, associated with CZA resistance in Klebsiella pneumoniae strains belonging to high-risk clones ST11 and ST16. Our finding suggests that novel mutations in KPC-2 are increasing in South America, which is a critical issue deserving active surveillance.

Ceftazidime/avibactam

Ertapenem

Carbapenem

Doripenem

10.1128/spectrum.00374-23

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