Yasue Hosoyamada

Chiba Prefectural University of Health Sciences

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Masako Yamada

Hyogo University of Health Sciences

Tatsuo Sakai

Juntendo University

Koichiro Ichimura

Juntendo University

Yoshiki Kobatake

Teikyo University

Keiichi Kuroda

Astellas Pharma (Japan)

Soichiro Kakuta

Juntendo University

Yuto Kawasaki

Juntendo University

Takayuki Miyaki

Juntendo University

Yoshie Yamauchi

Chiba Prefectural University of Health Sciences

康細

康恵細山田

Cooperative Institutions

Juntendo University

University of Utah

Universidade de São Paulo

Chiba Prefectural University of Health Sciences

Charité - Universitätsmedizin Berlin

Freie Universität Berlin

Chiba University

Mukogawa Women's University

University of Sassari

Humboldt-Universität zu Berlin

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Effect of Fish Oil on Concentrations of Serum and Liver Lipids of Rats Fed the Diet Containing Egg Yolk Phospholipids.

Nippon Eiyo Shokuryo Gakkaishi (1997)

Keiichi Kuroda Yasue Hosoyamada Yoshiki Kobatake

ラットへの高Chol飼料投与は血清や肝臓のChol濃度上昇を起こすが, この飼料に5%卵黄リン脂質を添加すると, 血清, 肝臓Chol濃度は抑制され, 糞中への見かけのChol排泄率は増加した。魚油のみの添加では血清Chol濃度は低下したが, 肝臓Chol濃度の低下は起こらず, Chol排泄率にも影響しなかった。さらに, この飼料に卵黄リン脂質と魚油を同時に添加すると, 血清Chol濃度は低下した。しかし, Chol排泄率はリン脂質のみの添加より, むしろ低下した。魚油と似た脂肪酸組成をもつオキアミリン脂質を添加した飼料でもChol排泄率は低下した。しかし, 魚油の2倍のDHA濃度のDHA濃縮油はサフラワー油と同様にリン脂質によるChol排泄率上昇を抑制する効果を示さなかつた。これらの結果から, 卵黄リン脂質と魚油の同時添加における血清Chol低下作用は, 卵黄リン脂質は魚油脂肪酸と共同して, 糞中へのChol排泄以外の機作によって起こる可能性を示唆していた。また, その作用がDHAなどの高度不飽和脂肪酸によるのかどうか明らかではなかった。

Yolk

10.4327/jsnfs.50.25

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Effects of Dietary Fish Oil and Apple Polyphenol on the Concentration Serum Lipids and Excretion of Fecal Bile Acids in Rats

Journal of Nutritional Science and Vitaminology (2017)

Yasue Hosoyamada Masako Yamada

We studied the effects of fish oil and apple polyphenol combined with a high cholesterol diet in rats, and assessed serum and liver lipids concentrations, serum oxidative stress and fecal bile acid excretion. Young male rats were fed a diet containing the control (Control), apple polyphenol (AP), fish oil (FO) or fish oil+apple polyphenol (FO+AP) for 4 wk. The control diet contained a lard component. Posterior abdominal wall fat and testicle peripheral fat weights decreased in the FO+AP group compared to the AP group. The concentration of total cholesterol in the serum and liver decreased in the FO group and the FO+AP group compared to the Control and the AP groups. The concentration of adiponectin and biological antioxidant potential in the serum increased in the FO group compared to the other groups. The diacron-reactive oxygen metabolites in serum decreased in the FO group and the FO+AP group compared to the Control and the AP groups. The bile acid excretion in feces increased in the AP group, the FO group and the FO+AP group compared to the Control group. These results suggested that the combination of fish oil and apple polyphenol in the diet improved serum and liver lipids, which should assist in the prevention and improvement of metabolic syndrome.

10.3177/jnsv.63.21

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Effect of Dietary Rapeseed Phospholipids on Concentrations of Serum and Liver Lipids in Rats.

Nippon Eiyo Shokuryo Gakkaishi (1993)

Yasue Hosoyamada Yuki FURUI Keiichi Kuroda Yoshiki Kobatake

精製菜種リン脂質の血清と肝臓脂質濃度に及ぼす作用を他のリン脂質と比較検討するために, ラットに5%リン脂質添加飼料を投与し, 高コレステロール付加飼料と普通飼料投与時について, 血清, 肝臓中の脂質濃度に及ぼす作用を検討した。1) 高コレステロール飼料条件において, 菜種リン脂質は大豆, 卵黄の各リン脂質と同様にオリーブ油 (対照) と比較し, 強い血清コレステロール上昇抑制作用を示した。また, 肝臓の総コレステロール濃度では菜種リン脂質は他のリン脂質同様に低下作用を示した。みかけのコレステロ一ル糞中排泄率はいずれのリン脂質投与でも対照の2倍以上に増加していた。2) 4週間の普通飼料投与条件で大豆油 (対照) との比較において, 菜種リン脂質は成長に障害は認めなかった。菜種, 大豆の各リン脂質投与では血清総コレステロールとHDL-コレステロールの各濃度は低下したが, 総コレステロールに対するHDL-コレステロ一ルの比率は菜種リン脂質では低下しなかった。また, いずれのリン脂質投与でも肝臓総コレステロ一ルは低下した。これらの結果から, 菜種リン脂質は他の大豆, 卵黄リン脂質とリン脂質組成, 脂肪酸組成がかなり異なっていたが, 血清と肝臓脂質に対して似た作用をもつことを認めた。

10.4327/jsnfs.46.495

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Influence of Differences of Rat Strain on Serum and Liver Lipid Levels

Yoshiki Kobatake Yasue Hosoyamada

Strain (injury)

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Effect of Dietary Egg Yolk Phospholipids Added Ethanolon Concentration of Serum and Liver Lipids of Rats

Journal for the Integrated Study of Dietary Habits (2011)

Yasue Hosoyamada Yoshie Yamauchi Masako Yamada

We studied the effects of the egg yolk phospholipids (EggPL) with 10% added ethanol on the serum and liver lipid concentrations, and main fatty acids in the liver of rats. Rats were fed a diet containing Lard, EggPL, Lard+ethanol or EggPL+ethanol for 3 weeks.The control group was the Lard group. The concentration of serum phospholipid decreased in the EggPL group compared to the control group. The concentration of total serum cholesterol and triglyceride indicated a tendency to decrease in the EggPL group compared to the control group. The concentration of total liver cholesterol decreased in the EggPL group compared to the control group, but no significant difference was observed between the Lard+Ethanol group and the EggPL+Ethanol group. The concentrations of the liver triglyceride that decreased in the EggPL group were lower than those in the control group, and those in the EggPL+ethanol group were lower than those in the Lard+ethanol group. The concentrations of fatty acids in the liver were found to decrease in C16:0 and to increase in C18:2. These results suggested that the ethanol diets containing the EggPL appear to effectively decrease the concentration of serum and liver lipids. The egg yolk phospholipids, similar to the soybean phospholipids, were expected to prevent dyslipidemia and a fatty liver.

Yolk

Soybean oil

Dyslipidemia

10.2740/jisdh.22.153

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Three-dimensional imaging of podocyte ultrastructure using FE-SEM and FIB-SEM tomography

Cell and Tissue Research (2019)

Takayuki Miyaki Yuto Kawasaki Yasue Hosoyamada Takashi Amari Mui Kinoshita

Abstract Podocytes are specialized epithelial cells used for glomerular filtration in the kidney. They can be divided into the cell body, primary process and foot process. Here, we describe two useful methods for the three-dimensional(3D) visualization of these subcellular compartments in rodent podocytes. The first method, field-emission scanning electron microscopy (FE-SEM) with conductive staining, is used to visualize the luminal surface of numerous podocytes simultaneously. The second method, focused-ion beam SEM (FIB-SEM) tomography, allows the user to obtain serial images from different depths of field, or Z-stacks, of the glomerulus. This allows for the 3D reconstruction of podocyte ultrastructure, which can be viewed from all angles, from a single image set. This is not possible with conventional FE-SEM. The different advantages and disadvantages of FE-SEM and FIB-SEM tomography compensate for the weaknesses of the other. The combination renders a powerful approach for the 3D analysis of podocyte ultrastructure. As a result, we were able to identify a new subcellular compartment of podocytes, “ridge-like prominences” (RLPs).

Glomerulus

10.1007/s00441-019-03118-3

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Investigation of bacterial contamination and usage survey of water-bottles

Journal for the Integrated Study of Dietary Habits (2016)

Masako Yamada Misa Matsuo Yasue Hosoyamada

In recent years, many people have been carrying water-bottles and drink beverages from these bottles at any time. We investigated the microorganisms of both the bottle’s mouth and the bottle’s beverages to understand the sanitary condition. Standard plate counts of 103-105 cfu/cm2 and 104-107 cfu/mL were detected on the mouth of the water-bottle and in the beverages, respectively. Coliform bacteria also were detected, the counts of which were less than the standard plate counts. The causes of the microbial condition were insufficient washing of the water-bottle and mixing of oral bacteria. It is necessary to keep the bottle at a low temperature and consume the beverage as soon as possible.

Water bottle

Bottled water

Coliform bacteria

10.2740/jisdh.27.66

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The ultrastructure of periductal connective tissue and distinctive populations of collagen fibrils associated with ductal epithelia of exocrine glands.

Archives of Histology and Cytology (2003)

Yasue Hosoyamada Tatsuo Sakai

The ultrastructure of the connective tissue around the intraglandular ducts was observed in rat exocrine glands. Connective tissue with a dense population of collagen fibrils was found either around the ducts and blood vessels (perivasculoductal connective tissue; PVDCT) as in the lacrimal and salivary glands and liver, or solely surrounding the ducts (periductal connective tissue; PDCT) as in the exocrine pancreas, whereas the interlobular and intralobular interstitium of the glands-except for the liver-contained substantially fluid-filled spaces without collagen fibrils. The PVDCT and PDCT of these glands contained two populations of collagen fibrils-fibroblast-associated and epithelium-associated-although the development and density of these fibrils varied considerably in individual glands. Both populations of collagen fibrils were most developed in the lacrimal glands, in which the basal aspects of the ductal epithelium and the basement membrane showed considerable undulation associated with a distinctive peribasement membrane zone with amorphous matter and a small population of the fibrils. In the parotid and submandibular glands, both populations were distinct, though poorly developed. In the exocrine pancreas and hepatic Glisson's sheath, the two populations of collagen fibrils were moderately developed, and the basal aspects of the ductal epithelium were characterized by prominent invaginations in which the multilaminar basement membranes and the epithelium-associated collagen fibrils were frequently engulfed. These observations provide evidence that the two populations of collagen fibrils around the ducts are found universally in exocrine glands, and support the hypothesis of the collagen fibril-synthesizing and -secreting ability of ductal epithelial cells.

Anchoring fibrils

Nasal glands

10.1679/aohc.66.407

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Effects of Dietary Apple Polyphenol on Alcohol-induced Dyslipidemia in Obese Zucker Rats

Japanese Journal of Complementary and Alternative Medicine (2016)

Yasue Hosoyamada Masako Yamada

目的: 肥満ラットに, 高コレステロール食とアルコールを摂取し, リンゴポリフェノール(Apple polyphenol:AP）の添加が脂肪蓄積量や脂質異常症に及ぼす影響を明らかにすることを目的とした．方法:5週齢肥満モデル雄ラットを購入し, 4週間飼育した．実験飼料は, 高コレステロール食と10 %アルコール摂取で, リンゴポリフェノール無添加をControl群とし, リンゴポリフェノール添加群をAP群とした．臓器重量, 血清脂質濃度などの測定を行った．結果: 体重増加量, 総飼料摂取量に差は見られなかった．後腹壁脂肪重量は, Control群よりAP群で低下傾向を示した．血清中の総コレステロール, トリグリセリド濃度および血清ALTは, Control群よりAP群で有意に低値を示した．結論：アルコール摂取肥満ラットにおいて, リンゴポリフェノールは, 血清脂質濃度を低下することが明らかとなった．リンゴポリフェノール摂取が, 脂質代謝異常や脂肪肝の予防に役立つことが期待される．

Dyslipidemia

10.1625/jcam.13.63

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Effect of dietary DHA concentrate with high cholesterol to the apparent iron absorption rate in rats

Journal for the Integrated Study of Dietary Habits (2012)

Yasue Hosoyamada Masako Yamada

We studied the effects of the docosahexsaenoic acid concentrate (DHA) along with a high cholesterol (HC) diet given to rats on the epididymal fat pad weight, the serum and liver iron concentrations, and the apparent iron absorption rate. Young male rats were fed a diet containing 10% Butter, 10% DHA, 10% Butter+HC or 10% DHA+HC for 3 weeks. The epididymal fat pad weight decreased in the DHA group compared to the Butter group. The concentration of serum total cholesterol decreased in the DHA group compared to the Butter group, similar to the DHA+HC group when compared to the Butter+HC group. The concentration of serum iron increased in the DHA group compared to the Butter group and the concentration of liver iron decreased in the Butter+HC group and DHA+HC group compared to the Butter group and DHA group. It was clarified that the apparent absorption rates in the observed Butter+HC group and DHA+HC group were higher than the Butter group and DHA group fed the experimental diet for the 1-14 days. However the DHA group was lower than the Butter group fed the experimental diets for the 14-21 days. These results suggest that the concentration of total cholesterol decreased in the DHA+HC group compared to the Butter+HC group, and the accumulation of adipose tissue appeared to decrease in the DHA diet compared to the Butter group. From the apparent absorption rate for each week, it was clarified that the accumulation of iron was changed by the fatty acid composition of the sample lipids used in the experiment and different feeding periods. Furthermore, we are considering the iron intake by the body.

10.2740/jisdh.23.77

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