To study the chemical constituents of Hemsleya chensnsis.Various chromatographic techniques were used to separate and purify the constituents. The spectral data (MS, NMR) were obtained for structure elucidation.Eight known triterpenoid saponins were isolated from the root of H. chensnsis. Their structures were elucidated as dihydrocucurbitacin B (1), 25-O-acetyl-dihydrocucurbitacin F (2), cucurbitacin F (3), 3-O-(6'-ethyl ester) -beta-D-glucuropyranosyl oleanolic acid-28-O-alpha-L-arabinopyranoside (4), oleanolic acid-3-(6'-methyl ester) -beta-D-glucuropyranosyl (1-3) -alpha-L-arabinopyranoside (5), oleanolic acid-28-O-beta-D-glucopyranosyl-(1-6) -beta-D-glucopyranoside (6), 3-O-(6'-methyl ester) -beta-D-glucuropyranosyl oleanolic acid-28-O-beta-D-glucopyranosyl-(1-6) -beta-D-glucopyranoside (7), 3-O-(6'-methyl ester) -beta-D-glucuropyranosyl (1-2) -beta-D-glucopyranoside-oleanolic acid-28-O-beta-D-glucopyranoside (8).All compounds except for 2 were isolated from H. chensnsis for the first time.
Cancer metastasis is the major reason for cancer related deaths, and the mechanism of cancer metastasis still unclear. RPLP1, a member of a group of proteins known as ribosomal proteins, is associated with tumorigenesis and primary cell immortalization and is involved in cellular transformation. However, the expression and potential function of RPLP1 in TNBC remain unclear.The expression of RPLP1 in TNBC tissues and cell lines were detected with Real-Time PCR and western blotting. 81 cases of TNBC tissue samples and adjacent non-tumor tissue samples were tested by immunochemistry to determine the correlation between the RPLP1 expression and clinicopathological characteristics. In vitro, we determined the role and mechanistic pathways of RPLP1 in tumor metastasis in TNBC cell lines.In this study, we detected high levels of RPLP1 expression in TNBC samples and cell lines. RPLP1 is upregulated in triple-negative breast cancer (TNBC) tissues and cells, and high expression levels correlate with an increased risk of recurrence and metastasis. Furthermore, high RPLP1 expression was associated with a poor prognosis and was an independent prognostic marker for TNBC. In RPLP1-induced cancer metastasis, RPLP1 may increase cancer cell invasion, which is likely the result of its effect on the cancer cell epithelial-mesenchymal transition.Altogether, our findings indicate RPLP1 is a poor prognostic potential biomarker and anti-metastasis candidate therapeutic target in triple-negative breast cancer.
To investigate the effects of Bisphenol A (BPA) on prolactin (PRL) release, pituitary cell proliferation, prolactinoma formation in estrogen-sensitive Fischer 344 (F344) rats.Four-week-old female F344 rats were orally administered with different concentrations of BPA or intraperitoneal injection of estradiol benzoate (estradiolbenzoate, E2) for 12 weeks. Bodyweight, blood RPL level and pituitary weights were observed and recorded. Real-time PCR, western blot and immunohistochemistry analysis were used to detect the mRNA and protein levels of the proliferation markers, including proliferating cell neclear antigen (PCNA), pituitary tumor-transforming gene (PTTG) and its relevant marker ERα. Plasma and urine BPA concentration in patients with prolactinoma and healthy participants were measured as well.Body weights of the rats treated with BPA were significantly decreased compared with those in the control group. The plasma PRL level and the pituitary weights of the rats were higher than those in the control group after BPA treatment. Compared with the control group, the pituitary mRNA and protein expression levels of PCNA and PTTG were significantly increased after BPA treatment. Moreover, ERα expression level was enhanced by the treatment of BPA in comparison with that of the control group. Finally, the plasma BPA concentration in the prolactin tumor patients was significantly higher than that in the healthy participants.BPA can significantly promote pituitary cell proliferation and prolactin secretion in F344 rats, which may have impact on the proliferation and secretion of pituitary cell function through the ERα pathway.
Objective To explore the different surgical procedures for uterine fibroids in patients with ovarian function.Methods The hospital in 2009—2011 received patients with uterine fibroids menopausal transition 150 cases and the use of different surgical methods for the surgical treatment of patients before surgery,after surgery,3 months and 6 months in serum estrogen hormone,testosterone,follicle-stimulating hormone,luteinizing hormone and pituitary prolactin expression were analyzed.Results Compared with before surgery,and myomectomy group,compared to total hysterectomy subtotal hysterectomy group and the group of patients aged 40~44 years 3 months after surgery significantly decreased levels of estrogen,follicle-stimulating hormone levels rise,showed significant changes(P0.05).Patients 6 months after surgery,hysterectomy group and subtotal hysterectomy group,serum levels of various hormones before surgery and myomectomy group,compared to no statistically significant(P0.05).And before surgery,and myomectomy group,compared to total hysterectomy subtotal hysterectomy group and the group of patients aged 45~55 years old in 3 months after surgery,hormonal changes was no significantly different(P0.05).45~50 years-old patient 6 months after surgery,hysterectomy group and the subtotal hysterectomy group,serum estrogen levels were significantly decreased follicle-stimulating hormone significantly increased,and before surgery and myomectomy group with significant difference(P0.05).Conclusion 45~50 years-old menopausal transition for patients with hysterectomy on ovarian function has significant impact will occur early ovarian decline.
Abstract Introduction: The widespread of implementation of computed tomography (CT) has resulted in a drastic increase in the detection of small pulmonary nodules presenting as ground glass opacities (GGOs). Many of these GGOs are atypical adenomatous hyperplasia (AAH), adenocarcinoma in situ (AIS), minimally invasive adenocarcinoma (MIA), and sometimes invasive adenocarcinoma (IAC). The concept of a continuum between the precursor lesions AAH and AIS to MIA and frankly invasive IAC is backed by a wealth of recent data showing a gradual decrease in overall survival from 100% for AAH, AIS and MIA to moderately lower rates for IAC. A growing body of evidence suggests that the immune response is a significant determinant of cancer progression. However, little is known about the immune microenvironment features in the early stages of lung carcinogenesis. Methods: A total of 40 tumor tissue samples including 10 AAH, 10 AIS, 10 MIA and 10 early IAC from 40 patients who underwent surgical resection of pulmonary nodules at Yancheng No.1 People's Hospital, China were included. Immunohistochemistry (IHC) staining for the expression of CD3, CD4, CD8 and programmed death ligand 1 (PD-L1) to assess the role of T-cell immune surveillance in these pre- and early lung cancers were performed. Results: We observed CD3, CD4, CD8 and PD-L1 positivity in AAH, AIS, MIA and IAC, suggesting that immunoediting has started before the invasion. Higher expressions of CD3, CD4, CD8 and PD-L1 in IAC implying tumor invasion. Conclusions: Our preliminary results of the immune subgroups provide evidence for immune recognition in pulmonary premalignancy and are critical for understanding tumorigenesis and the development of effective preventive and interception strategies. Associations with genomic landscape is underway. Citation Format: Liting Guo, Weiwei Shao. Immune microenvironment features of lung adenomatous premalignancy in a Chinese cohort [abstract]. In: Proceedings of the Annual Meeting of the American Association for Cancer Research 2020; 2020 Apr 27-28 and Jun 22-24. Philadelphia (PA): AACR; Cancer Res 2020;80(16 Suppl):Abstract nr 1002.
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