Shiwani Mandhania

Chaudhary Charan Singh Haryana Agricultural University

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R. S. Sangwan

Central Institute of Medicinal and Aromatic Plants

Ajay Pal

Chaudhary Charan Singh Haryana Agricultural University

S.S. Siwach

Chaudhary Charan Singh Haryana Agricultural University

O. Sangwan

Chaudhary Charan Singh Haryana Agricultural University

V.S. Mor

Chaudhary Charan Singh Haryana Agricultural University

Pin ki

Sombir Singh

Bidhan Chandra Krishi Viswavidyalaya

Neha Rohila

Chaudhary Charan Singh Haryana Agricultural University

Vinod Saharan͙

Maharana Pratap University of Agriculture and Technology

Dharam Paul Chaudhary

ICAR-Indian Institute of Maize Research

Cooperative Institutions

Chaudhary Charan Singh Haryana Agricultural University

Maharana Pratap University of Agriculture and Technology

Swami Keshwanand Rajasthan Agricultural University

ICAR-Indian Institute of Maize Research

Indian Council of Agricultural Research

Indian Agricultural Research Institute

University of Delhi

Lovely Professional University

Central Institute of Post-Harvest Engineering and Technology

Punjab Agricultural University

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Response of biochemical constituents under different sowing periods and their correlation with seed germination in Gossypium arboreum L

International Journal of Chemical Studies (2018)

Sunayana RS Sangwan Shiwani Mandhania

A two-year field experiment was conducted at the Research Farm of Cotton Section, Department of Genetics and Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar to illustrate the effects of weather parameters on biochemical parameters viz. protein content, oil content, sugar content and gossypol content of desi cotton varieties HD 123, HD 324 and HD 432 grown in different environmental conditions. Experimental material was planted on 10 April, 15 May and 5 June during 2015 while on 26 April, 5 May and 2 June during 2016 in a Randomized Block Design (RBD) with six replications. The flowering during both the years started from early July and completed by end of September during both the years. Flower were tagged on weekly basis and seed cotton picked from them were ginned separately and thus this seed was used to estimate the biochemical parameters of different sowing dates and their correlation with seed germination. The response of various biochemical constituents differed in different sowing periods. Early sown condition was suitable for the development of biochemical traits in desirable direction. It leads better seed development which resulted in increased the seed. Seed germination with different biochemical parameters showed positive correlation except gossypol content which showed negative correlation.

Gossypium

Gossypol

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Postharvest handling of ethylene with oxidative and absorptive means

Journal of Food Science and Technology (2023)

Sunil Kumar Ramesh Kumar Bhushan Bibwe Prerna Nath Rajesh K. Singh

10.1007/s13197-023-05777-1

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Citations (1)

Inter-relationship among nutritional quality parameters of maize (Zea mays) genotypes

The Indian Journal of Agricultural Sciences (2012)

Dharam Paul Chaudhary Sapna Sapna Shiwani Mandhania Ravi Kumar

The present study was taken up to understand the inter-relationship among various nutritional components of maize (Zea mays L.) kernel. For this purpose, 23 elite maize genotypes received from different centres of All India Coordinated Research Projects on Maize during 2009 and 2010 were used. The samples were ground to powder and processed for the estimation of various nutritional quality parameters such as protein quality, starch, oil and carotenoids and the data was correlated to find the interrelationship between these components. The results showed that the protein content exhibited a significant negative correlation with two important essential amino acids such as tryptophan and lysine. An inverse correlation was found between starch and oil indicating that breeding for high oil maize may lead to lower grain yield. A significant positive correlation was observed between oil content and 100-kernel weight postulating that although increase in oil down-regulate the starch content, the total grain yield, however, would remain unaffected. Protein content showed a non-significant negative correlation with 100-kernel weight. A significant positive correlation was noticed between protein and starch. Another important finding of this study is the positive correlation observed between oil and fat soluble total carotenoids. Breeding for high oil, therefore, would have the added advantage of biofortification of maize. The findings will help to develop improved maize having an answer to better nutrition.

Biofortification

Negative correlation

10.56093/ijas.v82i8.23049

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Citations (10)

Genetic variability in nutritional profile of elite maize genotypes.

Indian Journal of Agricultural Biochemistry (2012)

Om Prakash Sapna Sapna Shiwani Mandhania Avinash Singode Dharam Paul Chaudhary

The present investigations were conducted to explore the genetic variability in nutritional profile of twenty three elite maize genotypes received from various AICRP maize centres located across India. Protein content did not show any significant variation. However, a significant variation was observed in the protein quality viz; tryptophan (0.30 to 0.77) and lysine (1.14 to 2.96 per cent of protein) content in present genotypes. Starch content did not vary significantly. Oil content in seeds was found to vary from 3–5 per cent on dry weight basis. Wide variability was observed in the carotenoid and β carotene content. Carotenoid content ranged from 16.05 to 32.33 percent, whereas the β -carotene was found to vary from 1.08 to 8.6 per cent. The variability observed in the nutritional profile of present genotypes could be utilized for developing nutritionally superior maize cultivars.

Genetic Variability

Non-invasive ventilation

Carotene

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Antioxidant defense mechanism under salt stress in wheat seedlings

Biologia Plantarum (2006)

Shiwani Mandhania S. Madan Veena Sawhney

The present study was carried out to study the effect of salt stress on cell membrane damage, ion content and antioxidant enzymes in wheat (Triticum aestivum) seedlings of two cultivars salt-tolerant KRL-19 and salt-sensitive WH-542. Seedlings (4-d-old) were irrigated with 0, 50 and 100 mM NaCl. Observations were recorded on the 3rd and 6th day after salt treatment and 2nd day after salt removal. The relative water content declined with induction of salt stress, more in WH-542 than in cv. KRL-19. K+/Na+ ratio in KRL-19 was higher than in WH-542. WH-542 suffered greater damage to cellular membranes due to lipid peroxidation as indicated by higher accumulation of H2O2, MDA and greater leakage of electrolytes than KRL-19. The activities of catalase, peroxidase and ascorbate peroxidase and glutathione reductase increased with increase in salt stress in both the cultivars, however, superoxide dismutase activity declined. Upon desalanization, partial recovery in the activities of these enzymes was observed in KRL-19 and very slow recovery in WH-542.

Glutathione reductase

10.1007/s10535-006-0011-7

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Citations (258)

Pioneering Nit Gene Exploitation to Develop Molecular Diagnostic Assay for Rapid Detection of Cotton Root Rot Incitant, Macrophomina phaseolina (Tassi) Goid, in Field Soil

Journal of Basic Microbiology (2024)

Anil Kumar Saini Mukesh Kumar Karmal Singh Mukul Kumar Bhambhu Rohit Nain

ABSTRACT Cotton root rot caused by Macrophomina phaseolina pose a significant threat to cotton production, leading to substantial yield and quality losses. Early and accurate diagnosis of this pathogen in soil is crucial for effective disease management. This study presents a pioneering investigation into the utilization of the nit gene encoding nitrilase for the development of a molecular diagnostic assay aimed at the rapid detection of M. phaseolina in field soils. The methodology involved the design and validation of primers targeting the Nit gene sequence, followed by the optimization of PCR conditions for efficient amplification. Leveraging state‐of‐the‐art molecular techniques, the assay offers a novel protocol to accurately identify the presence of M. phaseolina in soil with high sensitivity and specificity. The specificity of the designed primers was confirmed through PCR amplification using DNA from M. phaseolina and other related fungi. Sensitivity tests demonstrated that the PCR assay reliably detected M. phaseolina DNA at concentrations as low as 1 ng. Furthermore, the performance of the diagnostic assay was rigorously evaluated using field soil samples with a known status of M. phaseolina infection, demonstrating its reliability and efficacy in real‐world scenarios. This study introduces a novel molecular marker for the detection of M. phaseolina and offers a rapid and efficient means for screening M. phaseolina in large soil samples with minimal time and manpower.

Macrophomina phaseolina

Amplicon

Root rot

10.1002/jobm.202400325

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Citations (2)

Role of biochemical traits for resistance to cotton leaf curl disease (CLCuD) in Gossypium hirsutum L.

Journal of Pharmacognosy and Phytochemistry (2017)

Sonika Sonika RS Sangwan Shiwani Mandhania

Cotton leaf curl disease (CLCuD) is a major biotic constraint that can significantly reduce the crop productivity. Development of resistant variety is most effective method to tackle with this and the successful exploitation of source of resistance requires information on the biochemical traits imparting resistance to CLCuD. We examined the role in imparting resistance to CLCuD of different biochemical traits including total sugar, phenols, gossypol, tannin, crude protein contents and enzymatic activities of peroxidase and polyphenoloxidases in resistant and susceptible cotton genotypes and their F1s at two different growth stages by using their standardized methods. Four genetically diverse cotton genotypes i.e. two resistant (GCH 3 and H 1353) and two highly susceptible to CLCuD (HS 6 and RST 9) were identified from the germplasm during 2013; four F1 hybrids were developed by crossing these genotypes and raised during kharif 2014 and finally evaluated under field conditions in 2015. Two factor analysis depicted that the amount of total phenol, gossypol, tannin, protein and activities of peroxidase & polyphenoloxidase was significantly higher in leaves of resistant genotypes than susceptible genotypes at different growth stages. Also, the significantly higher concentrations of these constituents in resistant genotypes after infection suggested that there is a correlation between increased levels of these constituents and plant resistance. So, these constituents might play an active role in imparting resistance to CLCuD and provide defense mechanism to plants which suggested that these traits could serve as potential biochemical markers and further may be used for early screening of germplasm lines.

Germ plasm

Leaf curl

Gossypol

Phytoplasma

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Morpho-physiological and biochemical responses of cotton (Gossypium hirsutum L.) genotypes upon sucking insect-pest infestations

Physiology and Molecular Biology of Plants (2022)

Vikram Singh Shiwani Mandhania Ajay Pal Taranjeet Kaur Prakash Banakar

Morpho

Insect pest

Plant Physiology

10.1007/s12298-022-01253-w

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Citations (12)

Grey relational analysis based multiresponse optimization for WEDM of ZE41A magnesium alloy

International Journal of Lightweight Materials and Manufacture (2022)

Rajender Kumar Puneet Katyal Shiwani Mandhania

It is considered that corrosion of biodegradable implants causes the release of metal ions from the implant surface, leading to tissue inflammation and subsequent failure of the implant. The alloying composition of the material, as well as the surface features of the material, determines the rate of implant degradation. The present study analyzed the influence of the wire electrical discharge machining (WEDM) process parameters on the rate of material removal, the surface characteristics, and the rate of corrosion of the ZE41A Mg alloy. Weighing samples after one week of immersion in simulated body fluid (SBF) determined the corrosion rate. The experiments were conducted using a Taguchi L16 orthogonal array (OA). WEDM is a method of spark erosion that affects the surface characteristics of magnesium alloy samples by developing overlapping craters and surface oxides that affect the mineralization process. The discharge energy has a significant effect on the response characteristics of the WEDM process, namely rate of material removal (MRR), surface roughness (SR), and corrosion rate (CR), and a trade-off between these response properties is always required to achieve the best performance for biodegradable Mg-alloy implants. As a consequence, grey relational analysis (GRA) was employed to optimize MRR, SR, and CR simultaneously. The study discusses the significance of machining process parameters with the degradation behavior of biomedical implant materials in a simulated body fluid (SBF). According to the current study, the most influential parameters in multi-objective optimization are the Ip and Ton. ZE41A Mg alloy machined samples at optimum WEDM parameters enhanced performance in reduced surface roughness and low in-vitro corrosion rate for ZE41A Mg-alloy. By optimization, the mechanical integrity loss of bone design can be mitigated in relation to bone fracture healing time.

Electrical Discharge Machining

Grey relational analysis

Simulated body fluid

Surface Modification

10.1016/j.ijlmm.2022.06.003

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Citations (23)

Combining ability study on seed cotton yield and its components in desi cotton (Gossypium arboreum L.).

Journal of Cotton Research and Development (2016)

S. R. Pundir Somveer Nimbal R. S. Sangwan S. S. Siwach O. Sangwan

Gossypium

Gossypium barbadense