Seo-Gyeong Kim

Chungbuk National University

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Jiho Min

Jeonbuk National University

Simranjeet Singh Sekhon

The University of Texas Health Science Center at San Antonio

Sang‐Hee Lee

Kangwon National University

Woo‐Ri Shin

Chungbuk National University

Yang‐Hoon Kim

Chungbuk National University

Ji‐Young Ahn

Chungbuk National University

Yang-Hoon Kim

Gee Na Yang

Hojin Ryu

Chungbuk National University

Kyung-Hwan Kim

Chungbuk National University

Cooperative Institutions

Chungbuk National University

Jeonbuk National University

Korea University

Korea Research Institute of Bioscience and Biotechnology

Cheongju University

Pohang University of Science and Technology

Rural Development Administration

Seoul National University Hospital

Korea University of Science and Technology

Konkuk University

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Advances in pathogen-associated molecules detection using Aptamer based biosensors

Molecular & Cellular Toxicology (2013)

Simranjeet Singh Sekhon Seo-Gyeong Kim Sang‐Hee Lee Am Jang Jiho Min

Aptamer

10.1007/s13273-013-0039-7

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Citations (25)

Aptamer-Based Pathogen Monitoring for Salmonella enterica ser. Typhimurium

Journal of Biomedical Nanotechnology (2018)

Woo‐Ri Shin Simranjeet Singh Sekhon Seo-Gyeong Kim Seung Jae Rhee Gee Na Yang

Salmonella enterica ser. Typhimurium is a foodborne pathogen that causes salmonellosis. Symptoms of salmonellosis include fever, diarrhea, and gastroenteritis. Conventional culture methods for detecting foodborne bacterial pathogens require long incubation time, expensive immunoassay methods, and sample enrichment steps. The objective of this study was to develop an aptamer-based sandwich assay to detect S. enterica ser. Typhimurium. First, S. enterica ser. Typhimurium specific binding aptamers (S11 and S24) were obtained by whole-cell SELEX for high sensitivity and specificity detection using live S. enterica ser. Typhimurium. S11 and S24 aptamers were able to capture S. enterica ser. Typhimurium selectively and distinguish it from other species of Salmonella (S. enterica ser. Typhimurium, S. enterica ser. Choleraesuis, S. enterica ser. Dublin, and S. enterica ser. Enteritidis) and food-borne bacterial pathogens (Escherichia coli K12, Listeria monocytogenes, Shigella sonnei and Staphylococcus aureus) with KD values of 4.41×10-12 M and 3.75×10-11 M, respectively. S. enterica ser. Typhimurium. aptamer-based sandwich assay exhibited a linear response for sensing S. enterica ser. Typhimurium. cells at concentration ranging from 2×101 to 2×105 CFU/mL. This aptamer-based sandwich assay can be used for on-site detection of S. enterica ser. Typhimurium.

Salmonella enterica

Aptamer

10.1166/jbn.2018.2634

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Citations (28)

Rapid Detection of HbA 1c Using DNA Aptamer

한국생물공학회 학술대회 (2014)

Seo-Gyeong Kim Sang‐Hee Lee Woo‐Ri Shin Simranjeet Singh Sekhon Jiho Min

Aptamer

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