Haley Barnett

University of Louisiana at Monroe

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Mary Caldorera‐Moore

Louisiana Tech University

Jamie J. Newman

Louisiana Tech University

India Pursell

Tulane University

Nellie Perez

Louisiana Tech University

Abitha M. Heimbuck

Louisiana Tech University

John Bradley Cart

Duke University

Anna Whitehead

Louisiana State University Health Sciences Center New Orleans

Nicholas A. Peppas

The University of Texas at Austin

Laura Lee

University of Alberta

Tyler R. Priddy‐Arrington

Louisiana Tech University

Cooperative Institutions

Louisiana Tech University

The University of Texas at Austin

Tulane University

Louisiana State University

University of Alberta

Vanderbilt University Medical Center

Oceaneering International (United States)

Huawei German Research Center

Dell Children's Medical Center of Central Texas

Purdue University West Lafayette

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Combination of soluble factors and biomaterial scaffolds enhance human adipose-derived stem/stromal cell myogenesis

Biochemical and Biophysical Research Communications (2020)

Haley Barnett India Pursell Laura Lee Nellie Perez Mary Caldorera‐Moore

10.1016/j.bbrc.2020.06.107

Cite

Citations (3)

The Use of Biomimetic Hydrogels to Direct Stem Cell Differentiation for Tissue Engineering Applications

India Pursell Haley Barnett Anna Whitehead Mary Caldorera‐Moore Jamie J. Newman

Source

Cite

Citations (0)

Development of Responsive Chitosan–Genipin Hydrogels for the Treatment of Wounds

ACS Applied Bio Materials (2019)

Abitha M. Heimbuck Tyler R. Priddy‐Arrington Madison L. Padgett Claire B. Llamas Haley Barnett

Chronic wounds are characterized by an increased bacterial presence, alkaline pH, and excessive wound drainage. Hydrogel biomaterials composed of the carbohydrate polymer chitosan are advantageous for wound healing applications because of their innate antimicrobial and hemostatic properties. Here, genipin-cross-linked-chitosan hydrogels were synthesized and characterized, and their in vitro and in vivo performances were evaluated as a viable wound dressing. Characterization studies demonstrate that the developed chitosan-genipin hydrogels were able to neutralize an environmental pH, while averaging ∼230% aqueous solution uptake, demonstrating their use as a perfusive wound dressing. Bacterial activity studies demonstrate the hydrogels' ability to hinder Escherichia coli growth by ∼70%, while remaining biocompatible in vitro to fibroblast and keratinocyte cells. Furthermore, chitosan-genipin hydrogels promote an enhanced immune response and cellular proliferation in induced pressure wounds in mice. All together, these results reflect the potential of the developed hydrogels to be used as a proactive wound dressing.

Genipin

10.1021/acsabm.9b00266

Cite

Citations (78)

Poly (ethylene glycol) hydrogel scaffolds with multiscale porosity for culture of human adipose-derived stem cells

Journal of Biomaterials Science Polymer Edition (2019)

Haley Barnett Abitha M. Heimbuck India Pursell Rachel A. Hegab Benjamin J. Sawyer

Three-dimensional (3 D) hydrogel scaffolds are an attractive option for tissue regeneration applications because they allow for cell migration, fluid exchange, and can be synthesized to closely mimic the physical properties of the extracellular matrix environment. The material properties of hydrogels play a vital role in cellular migration and differentiation. In light of this, in-depth understanding of material properties is required before such scaffolds can be used to study their influence on cells. Herein, various blends and thicknesses of poly (ethylene glycol) dimethacrylate (PEGDMA) hydrogels were synthesized, flash frozen, and dried by lyophilization to create scaffolds with multiscale porosity. Environmental scanning electron microscopy (ESEM) images demonstrated that lyophilization induced microporous voids in the PEGDMA hydrogels while swelling studies show the hydrogels retain their innate swelling properties. Change in pore size was observed between drying methods, polymer blend, and thickness when imaged in the hydrated state. Human adipose-derived stem cells (hASCs) were seeded on lyophilized and non-lyophilized hydrogels to determine if the scaffolds would support cell attachment and proliferation of a clinically relevant cell type. Cell attachment and morphology of the hASCs were evaluated using fluorescence imaging. Qualitative observations in cell attachment and morphology of hASCs on the surface of the different hydrogel spatial configurations indicate these multiscale porosity hydrogels create a suitable scaffold for hASC culture. These findings offer another factor of tunability in creating biomimetic hydrogels for various tissue engineering applications including tissue repair, regeneration, wound healing, and controlled release of growth factors.

10.1080/09205063.2019.1612725

Cite

Citations (11)

Poly (ethylene glycol) hydrogel elasticity influences human mesenchymal stem cell behavior

Regenerative Biomaterials (2018)

Anna Whitehead Haley Barnett Mary Caldorera‐Moore Jamie J. Newman

Coordinated investigations into the interactions between biologically mimicking (biomimetic) material constructs and stem cells advance the potential for the regeneration and possible direct replacement of diseased cells and tissues. Any clinically relevant therapies will require the development and optimization of methods that mass produce fully functional cells and tissues. Despite advances in the design and synthesis of biomaterial scaffolds, one of the biggest obstacles facing tissue engineering is understanding how specific extracellular cues produced by biomaterial scaffolds influence the proliferation and differentiation of various cell sources. Matrix elasticity is one such tailorable property of synthetic scaffolds that is known to differ between tissues. Here, we investigate the interactions between an elastically tailorable polyethylene glycol (PEG)-based hydrogel platform and human bone marrow-derived mesenchymal stem cells (hMSCs). For these studies, two different hydrogel compositions with elastic moduli in the ranges of 50-60 kPa and 8-10 kPa were implemented. Our findings demonstrate that the different elasticities in this platform can produce changes in hMSC morphology and proliferation, indicating that the platform can be implemented to produce changes in hMSC behavior and cell state for a broad range of tissue engineering and regenerative applications. Furthermore, we show that the platform's different elasticities influence stem cell differentiation potential, particularly when promoting stem cell differentiation toward cell types from tissues with stiffer elasticity. These findings add to the evolving and expanding library of information on stem cell-biomaterial interactions and opens the door for continued exploration into PEG-based hydrogel scaffolds for tissue engineering and regenerative medicine applications.

Regenerative Medicine

Biomaterial

Elasticity

10.1093/rb/rby008

Cite

Citations (44)

Method and Techniques for Myogenic Differentiation of Human Adipose-Derived Stem Cells

Nellie Perez Summer Adams Laura Lee Haley Barnett John Bradley Cart

Source

Cite

Citations (0)

The Role of Notch Signaling and Environmental Factors in Adult Stem Cell Myogenic and Osteogenic Differentiation

John Bradley Cart Nellie Perez India Pursell Chris R. Miller Haley Barnett

Source

Cite

Citations (0)

Influence of extracellular cues of hydrogel biomaterials on stem cell fate

Journal of Biomaterials Science Polymer Edition (2022)

Haley Barnett Mariya Shevchuk Nicholas A. Peppas Mary Caldorera‐Moore

Tissue engineering is a multidisciplinary field that focuses on creating functional tissue through the combination of biomimetic scaffolds, a cell source, and biochemical/physiochemical cues. Stem cells are often used as the cell source due to their multipotent properties and autologous sourcing; however, the combination of physical and chemical cues that regulate their behavior creates challenges in reproducibly directing them to a specific fate. Hydrogel biomaterials are widely explored as tissue scaffolds due to their innate biomimetic properties and tailorability. For these constructs to be successful, properties such as surface chemistry and spatial configuration, stiffness, and degradability of the biomaterial used for the scaffold framework should be analogous to the natural environment of the tissue they are repairing/replacing. This is imperative, as cues from the surrounding extracellular matrix (ECM) influence stem cell behavior and direct cell differentiation to a specific lineage. Hydrogels offer great promise as tools to control stem cell fate, as researchers can modulate the degradation rates, mechanical properties, swelling behavior, and chemical properties of the biomaterial scaffold to mimic the instructive cues of the native ECM. Discussion of the advantages and challenges of utilizing hydrogel biomaterials as the basis of tissue scaffolds is reviewed herein, as well as specific examples of hydrogels in tissue engineering and advances in hydrogel research to achieve desired cell phenotypes.

Biomaterial

Decellularization

Cell fate determination

10.1080/09205063.2022.2054398

Cite

Citations (8)