Reaction products obtained from enzymatic acidolysis preparation of MLM-type(M,medium-chain fatty acids;L,long-chain fatty acids) structured lipids(SLs)were studied.The effects of KOH-hydroalcoholic solution to remove free fatty acids on recovery rate and composition of SLs were discussed,such as the volume of the KOH-hydroalcoholic solution,the ethanol-water ratio of the solution,the volume of the hexane and the volume of the hexane in second extraction step.The results indicated that the highest recovery rate was attained with the proportion of KOH-hydroalcoholic solution volume(mL) and reaction products mass(g) 8∶ 1,ethanol and water volume ratio 30∶ 70,hexane volume(mL) and reaction products mass(g) ratio 12∶ 1,in second extraction step hexane volume(mL) and reaction products mass(g) ratio 3∶ 1.Under these conditions the recovery rate of SLs was 94.35%,and triacylglycerol content and the acid value of SLs were 95.27% and 0.11 mg(KOH)/g,respectively.
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