Lactobacillus kosoi Chiou et al . 2018 and Lactobacillus micheneri McFrederick et al . 2018 are closely related, and they share 100 % 16S rRNA gene sequence similarity, 99.6 % pheS gene sequence similarity, 100 % rpoA gene sequence similarity, 97.3 % average nucleotide identity (ANI) value and 76.6 % in silico DNA–DNA hybridization ( is DDH) value, indicating that they represent the same species. Fatty acid methyl esters (FAME) analysis and phenotypic characterization also indicated that L. kosoi and L. micheneri are very similar. We propose L. kosoi Chiou et al . 2018 as a later heterotypic synonym of L. micheneri McFrederick et al . 2018. The taxonomic position of Lactobacillus plantarum subsp. argentoratensis in the L. plantarum group was re-examined using a polyphasic approach, including sequence analyses of 16S rRNA, pheS , rpoA and recA genes, average nucleotide identity analysis, in silico DNA–DNA hybridization, fatty acid methyl ester analysis and phenotypic characterization. Results of 16S rRNA gene sequence analysis indicated that L. plantarum subsp. argentoratensis was closely related to L. plantarum subsp. plantarum , L. pentosus and L. paraplantarum in the L. plantarum group, sharing 99.6–99.7 % 16S rRNA gene sequence similarities. Results of pheS , rpoA and recA gene sequence analyses indicated that L. plantarum subsp. argentoratensis was most closely related to L. plantarum subsp. plantarum , having 91.8 % pheS gene sequence similarity, 98.9 % rpoA gene sequence similarity and 93.1 % recA gene sequence similarity. L. plantarum subsp. argentoratensis DSM 16365 T shared 95.6 % ANI value and 62.9 % is DDH value with L. plantarum subsp. plantarum ATCC 14917 T . The low is DDH value confirmed that L. plantarum subsp. argentoratensis and L. plantarum subsp. plantarum represent two different species, rather than two different subspecies in the L. plantarum group. On the basis of the data from polyphasic characterization obtained in the present study and in previous studies, L. plantarum subsp. argentoratensis is elevated to the species level and represents a novel species of the genus Lactobacillus , for which the name Lactobacillus argentoratensis sp. nov. is proposed and the type strain is DKO 22 T (=CIP 108320 T =DSM 16365 T =JCM 16169 T ). Two novel Gram-stain-positive bacterial strains, designated 1206-1 T and F027-1-2, were isolated from traditional pickle in Heilongjiang Province, PR China, and from the intestinal tract of a honey bee ( Apis mellifera ) in Hubei Province, PR China, respectively. The two bacteria were characterized by a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, fatty acid methyl ester analysis, average nucleotide identity analysis, in silico DNA–DNA hybridization analysis and an analysis of phenotypic features. The results of 16S rRNA gene sequence analysis indicated that strains 1206-1 T and F027-1-2 were distantly related to Lactobacillus sharpeae , Lactobacillus hulanensis , Lactobacillus songhuajiangensis , Lactobacillus pantheris , Lactobacillus thailandensis , Lactobacillus camelliae , Lactobacillus jixianensis , Lactobacillus nasuensis , Lactobacillus baoqingensis , Lactobacillus manihotivorans and Lactobacillus porcinae . Strain 1206-1 T exhibited 94.2–96.4 % 16S rRNA gene sequence similarities, 69.5–83.3 % pheS gene sequence similarities and 73.1–90.3 % rpoA gene sequence similarities to type strains of phylogenetically related species. ANI and is DDH values between strain 1206-1 T and the type strains of phylogenetically related species were 52.7–73.7 % and 21.1–30.1 %, respectively. On the basis of the data obtained in the present study, a novel species, Lactobacillus zhaodongensis sp. nov. is proposed and the type strain is 1206-1 T (=CCM 8981 T =CCTCC AB 2019200 T =LMG 31620 T ).
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In 2018, Nouioui et al. transferred Bifidobacterium gallinarum and Bifidobacterium saeculare to Bifidobacterium pullorum as B. pullorum subsp. gallinarum and B. pullorum subsp. saeculare on the basis of digital DNA–DNA hybridization (dDDH) values. These two new subspecies were validated in the same year. However, we found that the genome (GenBank/ENA/DDBJ accession number JGZJ01000000) of B. pullorum used by Nouioui et al. in the dDDH analysis cannot represent B. pullorum . So, the taxonomic relationship between B. gallinarum , B. saeculare and B. pullorum should be re-examined. B. pullorum DSM 20433 T had 88.7–89.0 % average nucleotide identity (ANI) values and 37.5–38.0 % dDDH values to the type strains of B. gallinarum and B. saeculare , respectively, less than the threshold for species demarcation, confirming that B. pullorum represents a different species from B. gallinarum and B. saeculare . The ANI values and dDDH values between the type strains of B. gallinarum and B. saeculare were 96.7–96.9 % and 73.0–73.3 %, respectively, greater than the threshold for species demarcation, confirming that they represent the same species. Relatively low dDDH values (less than the 79–80 % threshold for subspecies demarcation) between the type strains of B. gallinarum and B. saeculare indicated that B. saeculare can be considered as a subspecies of B. gallinarum . On the basis of the results presented here, (i) B. gallinarum and B. saeculare should not be transferred to B. pullorum ; (ii) we propose B. saeculare Biavati et al. 1992 as a later heterotypic synonym of B. gallinarum Watabe et al. 1983 and as a new subspecies of B. gallinarum , for which the name B. gallinarum subsp. saeculare subsp. nov. is proposed.
Recently, the members of the genus Lactobacillus have been reclassified by Zheng et al. At the same time, Lactobacillus buchneri subsp. silagei , Lactobacillus zhaodongensis , Lactobacillus argentoratensis and Lactobacillus zeae have been successively and validly published, and Lactobacillus kosoi has also been reclassified. In the present study, all these species and subspecies were re-evaluated by 16S rRNA gene sequence analysis or a combination of 16S rRNA gene sequence analysis and phylogenomic treeing. On the basis of the results presented here, we propose to reclassify Lactobacillus zhaodongensis , Lactobacillus zeae , Lactobacillus argentoratensis and Lactobacillus buchneri subsp. silagei as Lacticaseibacillus zhaodongensis comb. nov., Lacticaseibacillus zeae comb. nov., Lactiplantibacillus argentoratensis comb. nov. and Lentilactobacillus buchneri subsp. silagei comb. nov., respectively and Apilactobacillus kosoi as a later heterotypic synonym of Apilactobacillus micheneri .
Seven novel lactic acid bacterial strains, isolated from traditional Chinese pickle, were characterized using a polyphasic approach, including 16S rRNA gene sequence analysis, pheS gene sequence analysis, rpoA gene sequence analysis, determination of DNA G+C content, determination of average nucleotide identity (ANI), in silico DNA–DNA hybridization (isDDH), fatty acid methyl ester (FAME) analysis and an analysis of phenotypic features. Strains 382-1T, 116-1AT, 381-7T, 203-3T, 218-3T and 398-2T were phylogenetically related to the type strains of Lactobacillus plantarum subsp. plantarum , Lactobacillus plantarum subsp. argentoratensis , Lactobacillus pentosus , Lactobacillus paraplantarum , Lactobacillus fabifermentans , Lactobacillus herbarum , Lactobacillus mudanjiangensis , Lactobacillus xiangfangensis , Lactobacillus plajomi and Lactobacillus modestisalitolerans , having 97.1–99.9 % 16S rRNA gene sequence similarities, less than 89.9 % pheS gene sequence similarities, less than 98.0 % rpoA gene sequence similarities, less than 91.2 % ANI values and less than 43.3 % isDDH values. Strain 778-3T was phylogenetically related to the type strains of Lactobacillus hokkaidonensis , Lactobacillus wasatchensis , Lactobacillus oligofermentans , Lactobacillus nenjiangensis , Lactobacillus vaccinostercus and Lactobacillus suebicus , exhibiting 97.0–99.4 % 16S rRNA gene sequence similarities, 78.2–82.1 % pheS gene sequence similarities, 80.0–91.5 % rpoA gene sequence similarities, less than 78.6 % ANI values and less than 22.9 % isDDH values. Based upon the data of polyphasic characterization obtained in the present study, seven novel species, Lactobacillus pingfangensis sp. nov., Lactobacillus daoliensis sp. nov., Lactobacillus nangangensis sp. nov., Lactobacillus daowaiensis sp. nov., Lactobacillus dongliensis sp. nov., Lactobacillus songbeiensis sp. nov. and Lactobacillus kaifaensis sp. nov., are proposed and the type strains are 382-1T (=NCIMB 15187T=CCM 8935T=LMG 31176T), 116-1AT (=NCIMB 15181T=CCM 8934T=LMG 31171T), 381-7T (=NCIMB 15186T=CCM 8930T), 203-3T (=NCIMB 15183T=CCM 8933T=LMG 31172T), 218-3T (=NCIMB 15184T=CCM 8932T=LMG 31173T), 398-2T (=NCIMB 15189T=CCM 8931T=LMG 31174T) and 778-3T (=NCIMB 15191T=CCM 8929T=LMG 31177T), respectively.
Thirteen Gram-stain-positive bacterial strains were isolated from Chinese traditional pickle and the gut of honeybee ( Apis mellifera ). These strains were characterized using a polyphasic taxonomic approach. The data demonstrated that 12 of the 13 strains represented eight novel species belonging to the genera Apilactobacillus , Secundilactobacillus , Levilactobacillus and Lacticaseibacillus ; strains HN36-1 T , 887-11 T , F79-211-2 T , 866-3 T , 6-5(1) T , 13B17 T , 117-1 T and ZW152 T were designated as the type strains. Based upon the data of polyphasic characterization obtained in the present study, eight novel species, Apilactobacillus nanyangensis sp. nov., Secundilactobacillus hailunensis sp. nov., Secundilactobacillus yichangensis sp. nov., Levilactobacillus andaensis sp. nov., Levilactobacillus wangkuiensis sp. nov., Levilactobacillus lanxiensis sp. nov., Lacticaseibacillus mingshuiensis sp. nov. and Lacticaseibacillus suilingensis sp. nov., are proposed and the type strains are HN36-1 T (=JCM 33867 T =CCTCC AB 2019385 T ), 887-11 T (=NCIMB 15201 T =CCM 8950 T =JCM 33864 T =CCTCC AB 2018396 T ), F79-211-2 T (=NCIMB 15254 T =JCM 33866 T =CCTCC AB 2019384 T ), 866-3 T (=JCM 33863 T =CCTCC AB 2019383 T ), 6-5(1) T (=NCIMB 15229 T =CCM 8977 T =JCM 33564 T =CCTCC AB 2019168 T ), 13B17 T (=NCIMB 15230 T =CCM 8979 T =JCM 33565 T =CCTCC AB 2019167 T ), 117-1 T (=NCIMB 15232 T =CCM 8980 T =JCM 33567 T ) and ZW152 T (=JCM 34363 T =CCTCC AB 2020299 T =LMG 32143 T =CCM 9110 T ), respectively.
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