Amaryllis H. Van Craenenbroeck

KU Leuven

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Assessing the retinal microvasculature in heart failure: a systematic review and clinical guidance

Inne Vanreusel Jan Van Eijgen Freya Jena Andreas B. Gevaert Amaryllis H. Van Craenenbroeck

eview question / Objective To summarize the current literature on retinal vascular imaging parameters in HF, with particular f o c u s o n p a t h o p h y s i o l o g y, t h e r a p e u t i c implications, and their potential use as markers of HF risk, severity and prognosis.Rationale Despite significant progress in pharmacological and device therapy of heart failure (HF), its long-term prognosis remains poor, a n d i t s p a t h o p h y s i o l o g y i s i n c o m p l e t e l y understood.Microvascular function is impaired early in the development and progression of many pathological processes like HF. Retinal imaging provides a unique opportunity for easy, noninvasive and early detection of systemic microvascular disease. Condition being studiedHeart failure is a chronic condition where the heart is unable to pump blood efficiently to meet the body's needs.This can result from various underlying conditions that damage or overwork the heart muscle, such as cardiovascular comorbidities (like high blood pressure or obesity), coronary artery disease, cardiomyopathies, arrhythmias, heart valve disease… Key characteristics of heart failure include: * Symptoms: Common symptoms include shortness of breath, fatigue, swollen legs, and rapid heartbeat.* Types: Heart failure is often classified into two main types: heart failure with reduced ejection fraction (HFrEF) and heart failure with preserved ejection fraction (HFpEF) INPLASY

10.37766/inplasy2024.5.0114

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SP286PLASMA BETA TRACE PROTEIN PREDICTS MORTALITY IN CKD: RELATION WITH ENDOTHELIAL DYSFUNCTION

Nephrology Dialysis Transplantation (2016)

Amaryllis H. Van Craenenbroeck Abdul Rashid Qureshi Ann‐Christin Bragfors‐Helin Per Simonsson Bengt Lindholm

Endothelial Dysfunction

TRACE (psycholinguistics)

10.1093/ndt/gfw165.03

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Next-generation protein analysis in the pathology department

Journal of Clinical Pathology (2019)

Melek Ahmed Glenn Broeckx Geert Baggerman Karin Schildermans Patrick Pauwels

Traditionally, immunohistochemistry (IHC) is used by pathologists to localise specific proteins or peptides in tissue slides. In the era of personalised medicine, however, molecular tissue analysis becomes indispensable for correct diagnosis, prognosis and therapeutic decision, not only on the DNA or mRNA level but also on the protein level. Combining molecular information with imaging presents many advantages. Therefore, matrix-assisted laser desorption/ionisation imaging mass spectrometry (MALDI IMS) is a promising technique to be added to the armamentarium of the pathologist. Here, we focus on the workflow, advantages and drawbacks of both MALDI IMS and IHC. We also briefly discuss a few other protein imaging modalities and give examples of applications.

MALDI imaging

Mass spectrometry imaging

Modalities

Molecular Pathology

Molecular Imaging

10.1136/jclinpath-2019-205864

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Citations (16)

P1611PRE-ANALYTICAL CONSIDERATIONS IN STUDYING CIRCULATING MICRORNA EXPRESSION: COMPARISON BETWEEN PAIRED EDTA PLASMA, EDTA WHOLE BLOOD AND PAXGENE BLOOD RNA TUBES

Nephrology Dialysis Transplantation (2020)

Veerle Wijtvliet Amaryllis H. Van Craenenbroeck Annemieke Smet Steven Van Laere Annick Massart

Abstract Background and Aims microRNA (miRNA) dysregulations have been related to pathological processes, including kidney disease. Relative stability in blood makes miRNAs attractive biomarkers. The current recommendation is to use fresh EDTA plasma samples (i.e. processed within 30 min. from sampling) to study circulating miRNA. However, cumbersome logistics might preclude broad implementation. Therefore, we investigated the potential of whole blood EDTA and PAXgene blood RNA tubes as alternative sources to study circulating microRNA expression profiling. Method Paired EDTA plasma, EDTA whole blood and PAXgene blood RNA tubes were obtained from 10 healthy adults (50% male). EDTA plasma samples were processed within 30 min. after sampling and immediately stored at -80°C. EDTA whole blood tubes and PAXgene tubes were kept at room temperature for 48 hours after sampling. Subsequently, the content of the EDTA whole blood samples was transferred to a 15 mL Falcon tube and stored at -80°C. PAXgene tubes were transferred to -20°C following the manufacturer’s protocol. Within 1 month of storage, all samples were thawed and miRNA was extracted using the Qiagen miRNeasy serum/plasma kit and subjected to RNA-sequencing (Oxford Genomics Centre). Based on the raw data, a count table was created using the online tool miRDeep* for the identification of both novel and known microRNAs. Subsequent downstream bio-informatic analyses approaches consisted of 1) unsupervised hierarchical clustering with principal component analysis (PCA); 2) calculation of differential miRNA expression using generalized linear models with differences considered significant if the false discovery rate-adjusted p-value was inferior to 10%. Results Initial assessment of the count table showed significant differences in the number of detected microRNAs. A median of 220 different microRNAs was detected in EDTA plasma samples versus 661 in PaxGene samples (p < 0.05) and 490 in EDTA whole blood samples (p < 0.05) (Figure 1A). We also found fewer novel miRNAs in EDTA plasma samples than in PAXgene samples (p < 0.001) and EDTA whole blood samples (p < 0.05). Low count microRNAs, defined as below 10 reads in more than 20% of the samples, were more abundant in Paxgene samples versus EDTA plasma samples (p = 0.0039), but this difference was not significant when comparing EDTA whole blood samples with EDTA plasma samples (Figure 1B). PCA analysis (Figure 1C) showed a clear separation of samples according to the blood collection method, strongly suggesting that the blood collection method predominantly determines the miRNA expression profile. Conclusion Bio-informatic analyses demonstrated different miRNA expression profiles according to three different blood collection methods, underpinning the importance of a standardized method for the collection of blood aimed at studying circulating miRNAs. As such, this study has important implications for the design of novel studies aiming to investigate circulating miRNAs.

Blood sampling

Blood plasma

10.1093/ndt/gfaa142.p1611

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FP746Associations between the measures of physical function, the risk of falls and nutritional status of haemodialysis patients: a cross-sectional study

Nephrology Dialysis Transplantation (2019)

Karsten Vanden Wyngaert Bert Celie Patrick Calders Sunny Eloot Els Holvoet

Cross-sectional study

10.1093/ndt/gfz106.fp746

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Non-Skeletal, as Opposed to Skeletal, Alkaline Phosphatase Associates with Mortality in Patients with CKD Not Yet on Dialysis

Journal of the American Society of Nephrology (2023)

Dieter Smout Hanne S. Joergensen Björn Meijers Sander Dejongh Étienne Cavalier

10.1681/asn.20233411s1395b

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An Intact Dopamine Sensitivity in the Brain: A Necessity to Recover Hyperprolactinemia and Galactorrhea in a Female Hemodialysis Patient?

Case Reports in Nephrology (2017)

Eva Philipse Ester Philipse Theodorus B. Twickler Amaryllis H. Van Craenenbroeck Marie M. Couttenye

A female hemodialysis patient with galactorrhea due to hyperprolactinemia was treated with different dialysis modalities to assess the effect on prolactin levels. A single session of both high-flux hemodialysis and hemodiafiltration resulted in decreased prolactin levels (16,6% and 77,2%, resp.). However, baseline prolactin levels measured immediately before the next dialysis session did not change markedly. After cabergoline treatment was started, prolactin levels normalized and galactorrhea disappeared. Thus, dopaminergic inhibition of prolactin secretion might be reduced in patients with end-stage renal disease. This dopaminergic resistance could be an important mechanism of hyperprolactinemia in hemodialysis patients and its subsequent treatment strategies.

Cabergoline

Galactorrhea

10.1155/2017/3729629

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Effect of Moderate Aerobic Exercise Training on Endothelial Function and Arterial Stiffness in CKD Stages 3-4: A Randomized Controlled Trial

American Journal of Kidney Diseases (2015)

Amaryllis H. Van Craenenbroeck Emeline M. Van Craenenbroeck Katrijn Van Ackeren Christiaan Vrints Viviane M. Conraads

Aerobic Exercise

Brachial artery

10.1053/j.ajkd.2015.03.015

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