Patrycja Kurowska

Jagiellonian University

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Agnieszka Rak

Jagiellonian University

Ewa Mlyczyńska

Jagiellonian University

Joëlle Dupont

Physiologie de la Reproduction et des Comportements

Monika Dawid

Jagiellonian University

Karolina Pich

Jagiellonian University

Natalia Respekta

Jagiellonian University

Kamil Dobrzyń

University of Warmia and Mazury in Olsztyn

Nina Smolińska

University of Warmia and Mazury in Olsztyn

Christelle Ramé

Physiologie de la Reproduction et des Comportements

Alix Barbe

Physiologie de la Reproduction et des Comportements

Cooperative Institutions

Jagiellonian University

Physiologie de la Reproduction et des Comportements

Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement

Centre National de la Recherche Scientifique

University of Warmia and Mazury in Olsztyn

Université de Tours

Centre Val de Loire

National Research Institute of Animal Production

Polish Academy of Sciences

Institut Français du Cheval et de l'Équitation

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Asprosin, a novel adipokine, in the mouse hypothalamus: expression and in vitro effect on GT1-7 cells proliferation and GnRH secretion. Preliminary data

Endocrine Abstracts (2023)

Dominika Wachowska Natalia Respekta Patrycja Kurowska Anna Błasiak Joëlle Dupont

Searchable abstracts of presentations at key conferences in endocrinology ISSN 1470-3947 (print) | ISSN 1479-6848 (online)

10.1530/endoabs.90.ep865

Cite

Citations (0)

Expression of vaspin and GRP78 in corpus luteum are dependent on estrous phase in Large White pigs

HAL (Le Centre pour la Communication Scientifique Directe) (2019)

Patrycja Kurowska Monika Dawid Ewa Mlyczyńska Wacław Tworzydło Joëlle Dupont

White (mutation)

Large white

Source

Cite

Citations (0)

Correction: Szymanska et al. The Effect of Visfatin on the Functioning of the Porcine Pituitary Gland: An In Vitro Study. Cells 2023, 12, 2835

Cells (2024)

Karolina Szymańska Edyta Rytelewska Ewa Zaobidna Marta Kieżun Marlena Gudelska

In the original publication [...]

10.3390/cells13201741

Cite

Citations (0)

In Vitro Effects of Vaspin on Porcine Granulosa Cell Proliferation, Cell Cycle Progression, and Apoptosis by Activation of GRP78 Receptor and Several Kinase Signaling Pathways Including MAP3/1, AKT, and STAT3

International Journal of Molecular Sciences (2019)

Patrycja Kurowska Ewa Mlyczyńska Monika Dawid Małgorzata Opydo‐Chanek Joëlle Dupont

Vaspin, a visceral adipose tissue-derived serine protease inhibitor, is expressed in the porcine ovary; it induces the activation of various kinases and steroidogenesis. The aim of this study was to examine the effect of vaspin on granulosa (Gc) proliferation, cell cycle regulation, and apoptosis. Porcine Gc was incubated with vaspin (0.01-10 ng/mL) for 24 to 72 h, proliferation was measured using alamarBlue assay, cell cycle progression was assessed using flow cytometry, and cyclin (D, E, and A) protein expression was measured using immunoblotting. Apoptosis was assessed by measuring caspase activity using Caspase-glo 3/7 assay. Furthermore, histone-associated DNA fragments levels were measured using a cell-death detection ELISA; BAX (bcl-2-like protein 4), BCL2 (B-cell lymphoma 2), caspases (-3, -8, and -9), p53 mRNA, and protein expression were assessed using real time PCR and immunoblotting. We found that vaspin significantly enhanced Gc proliferation and cell cycle progression into the S and G2/M phases and decreased apoptosis. We observed that siRNA silencing of the glucose-regulated protein (GRP78) receptor and pharmacological inhibitors of mitogen-activated kinase (MAP3/1/ERK1/2), Janus kinase (STAT3) and protein kinase B (AKT) blocked the ability of vaspin cell proliferation and enhanced caspase-3/7 activities. These results suggest that vaspin via mitogenic effect on porcine Gc acts as a new regulator of ovarian growth, development, or folliculogenesis.

10.3390/ijms20225816

Cite

Citations (34)

Deciphering The Transcriptional Activities of Genes Coding For Adipokines and Their Receptors in Porcine Ex Situ-Protected Mesenchymal Stem Cells Undergoing Adipogenic Differentiation

Annals of Animal Science (2024)

Joanna Romanek M. Samiec J. Jurkiewicz Patrycja Kurowska Agnieszka Rak

Abstract The aim of this study was to investigate the impact of the source of mesenchymal stem cells (MSCs) and their adipogenic derivatives on the relative abundances (RAs) noticed for mRNA transcripts of the selected adipokines (adiponectin and leptin) and their receptors. MSCs were isolated from bone marrow (BM) and subcutaneous adipose tissue (AT ) samples collected post mortem from a total of four gilts (each at the age of 6 months). The stemness of the MSCs was proven via recognizing their abilities to differentiate into adipocytes, osteoblasts, and chondrocytes. By using real-time PCR (RT -qPCR), the quantitative levels of transcriptional activity pinpointed for the adiponectin ( ADIPOQ ), adiponectin receptor 1 ( ADIPOR1) , adiponectin receptor 2 ( ADIPOR2) , leptin ( LEP ), and leptin receptor ( LEPR ) genes were comparatively analyzed between non-differentiated AT -MSCs and BM-MSCs and their cell counterparts undergoing differentiation into adipocytes. A significantly higher RA for ADIPOR1 transcripts was identified in the AT-MSCs compared to the BM-MSCs and their adipogenic derivatives (P≤0.05). Moreover, the quantitative levels of LEPR transcripts were shown to increase significantly among adipocytes originating from differentiated BM-MSCs compared to undifferentiated BM-MSCs, AT -MSCs, and AT -MSC-derived adipocytes (P≤0.05). Nonetheless, there was no significant inter-group variability in the RAs of the ADIPOQ , ADIPOR2 , or LEP mRNA transcripts (P>0.05). Cumulatively, the in vitro models focused on the identification and detailed exploration of transcriptomic signatures of undifferentiated pig BM- and AT -MSCs, and the molecular mechanisms underlying the adipogenic differentiation pathways of the above-indicated two distinct sources of stem cells were developed and optimized in the current investigation for the very first time. These ex vivo porcine models might confirm the expedited functional mobilization of ex situ -protected MSCs and their enhanced capacity to be transcriptionally reprogrammed into adipocytes due to physiopathological alterations in the expression profiles of adipokines and their receptors, which are prompted and progressed in obese and superobese women patients at the peri-pubertal period of ontogenesis. Finally, the models providing comprehensive molecular insights into ex situ -protected porcine BM- and AT -derived MSCs and their differentiated derivatives can be largely suitable for the biotechnologically assisted rescue and restitution of endangered representatives of rare native breeds of domestic pig.

10.2478/aoas-2024-0030

Cite

Citations (0)

THE USE OF CHITIN IN THE MOULTS AND EXOSKELETONS OF MEALWORMS (TENEBRIO MOLITOR) TO REMOVE CATIONIC DYES FROM AQUEOUS SOLUTIONS

Progress on Chemistry and application of Chitin and its Derivatives (2023)

Patrycja Kurowska Tomasz Jóźwiak Urszula Filipkowska Tadeusz Bakuła

We analysed the feasibility of using unmodified chitin exoskeletons and moults of mealworms (Tenebrio molitor) as adsorbents to remove cationic dyes – Basic Violet 10 (BV10) and Basic Red 46 (BR46) – from aqueous solutions. We evaluated the characteristics of the adsorption materials using Fourier-transform infrared spectroscopy and determining the pH of the point of zero charge (pHPZC); the pH effect on dye adsorption effectiveness; dye adsorption kinetics (pseudo–first order, pseudo–second order, and intramolecular diffus ion models); and the maximum adsorption capacity of the adsorbents (Langmuir 1 and 2 and Freundlich isotherms). BV10 adsorption on the tested adsorbents was the highest at pH 3, while adsorption of BR46 was highest at pH 6. The adsorption equilibrium time depended mainly on the dye type and its initial concentration; it was 150–210 min for BV10 and 120–150 min for BR46. The maximum adsorption capacity of mealworm exoskeletons reached 5.56 mg/g for BV10 and 31.53 mg/g for BR46, whereas mealworm moults exhibited a higher maximum adsorption capacity, reaching 6.44 mg/g for BV10 and 5.56 mg/g for BR46.

Mealworm

Cationic polymerization

10.15259/pcacd.28.006

Cite

Citations (3)

The Apelinergic System: Apelin, ELABELA, and APJ Action on Cell Apoptosis: Anti-Apoptotic or Pro-Apoptotic Effect?

Cells (2022)

Natalia Respekta Karolina Pich Monika Dawid Ewa Mlyczyńska Patrycja Kurowska

The apelinergic system comprises two peptide ligands, apelin and ELABELA, and their cognate G-protein-coupled receptor, the apelin receptor APJ. Apelin is a peptide that was isolated from bovine stomach extracts; the distribution of the four main active forms, apelin-36, -17, -13, and pyr-apelin-13 differs between tissues. The mature form of ELABELA-32 can be transformed into forms called ELABELA-11 or -21. The biological function of the apelinergic system is multifaceted, and includes the regulation of angiogenesis, body fluid homeostasis, energy metabolism, and functioning of the cardiovascular, nervous, respiratory, digestive, and reproductive systems. This review summarises the mechanism of the apelinergic system in cell apoptosis. Depending on the cell/tissue, the apelinergic system modulates cell apoptosis by activating various signalling pathways, including phosphoinositide 3-kinase (PI3K), extracellular signal-regulated protein kinase (ERK1/2), protein kinase B (AKT), 5’AMP-activated protein kinase(AMPK), and protein kinase A (PKA). Apoptosis is critically important during various developmental processes, and any dysfunction leads to pathological conditions such as cancer, autoimmune diseases, and developmental defects. The purpose of this review is to present data that suggest a significant role of the apelinergic system as a potential agent in various therapies.

Apelin

Intrinsic apoptosis

10.3390/cells12010150

Cite

Citations (9)

Polycyclic aromatic hydrocarbons in human granulosa cells: first in vivo presence and positive correlation with body mass index and in vitro ovarian cell steroidogenesis regulation

Environmental Toxicology and Pharmacology (2024)

Patrycja Kurowska L. Berthet Christelle Ramé Małgorzata Węgiel Anna Maślanka

Polycyclic Aromatic Hydrocarbons (PAHs) exposure leads to disorders reported in female infertility patients. Our hypothesis is that PAHs accumulate in granulosa cells (Gc) according to body mass index (BMI) and directly affects its functions. All 16 high-priority PAHs were in human FF, Gc and blood plasma with the highest concentration in Gc (GC-MS/MS). Their highest concentration was in obese Gc, except for acenaphthene and acenaphthylene, and positively correlated with BMI. In FF, we noted only positive correlation between naphthalene and BMI, whereas in blood plasma positive correlation between naphthalene, acenaphthene, pyrene and BMI. Phenanthrene and naphthalene but not fluoranthene inhibited totally steroidogenesis (ELISA), CYP19A1 mRNA expression (real-time PCR) and increased oxidative stress index and catalase expression in Gc independently on BMI. While all studied PAHs decreased Gc proliferation (BrdU assay) and viability (Cell Count kit-8 assay). Thus, Gc PAHs concentrations are positively correlated with BMI and alter ovarian functions.

Acenaphthene

Acenaphthylene

Fluoranthene

10.1016/j.etap.2024.104611

Cite

Citations (0)

Expression and Impact of Vaspin on In Vitro Oocyte Maturation through MAP3/1 and PRKAA1 Signalling Pathways

International Journal of Molecular Sciences (2020)

Patrycja Kurowska Ewa Mlyczyńska Anthony Estienne Alix Barbe I. Rajska

Oocyte maturation is a critical stage in embryo production and female reproduction. The aims of this study were to determine: (i) the mRNA and protein expression of vaspin and its receptor 78-kDa glucose-regulated (GRP78) in porcine cumulus–oocyte complexes (COCs) by real-time PCR and Western blot analysis, respectively, and their localisation by immunofluorescence; and (ii) the effects of vaspin on in vitro oocyte maturation (IVM) and the involvement of mitogen ERK1/2 (MAP3/1)- and AMPKα (PRKAA1)-activated kinases in the studied processes. Porcine COCs were matured in vitro for 22 h or 44 h with vaspin at a dose of 1 ng/mL and nuclear maturation assessed by Hoechst 33342 or DAPI staining and the measurement of progesterone (P4) level in the maturation medium. We showed that vaspin and GRP78 protein expression increased in oocytes and cumulus cells after IVM. Moreover, vaspin enhanced significantly porcine oocyte IVM and P4 concentration, as well as MAP3/1 phosphorylation, while decreasing PRKAA1. Using pharmacological inhibitors of MAP3/1 (PD98059) and PRKAA1 (Compound C), we observed that the effect of vaspin was reversed to the control level by all studied parameters. In conclusion, vaspin, by improving in vitro oocyte maturation via MAP3/1 and PRKAA1 kinase pathways, can be a new factor to improve in vitro fertilisation protocols.

In vitro maturation

10.3390/ijms21249342

Cite

Citations (14)

Expression and in vitro effect of phoenixin-14 on the porcine ovarian granulosa cells

Reproductive Biology (2023)

Patrycja Kurowska Ewa Mlyczyńska Julia Wajda Konrad Król Karolina Pich

10.1016/j.repbio.2023.100827

Cite

Citations (1)