Eiichi Takenouchi

Research Field

S-(β-Aminoethyl)-l-cysteine (SAEC), a sulfur analog of l-lysine, significantly inhibited the growth of wild-type strains of Candida species. The growth inhibition of C. pelliculosa depended on SAEC concentrations, but l-Iysine and l-α-aminoadipate restored growth effectively. SAEC-resistant mutants were induced from the wild-type strain of C. pelliculosa by ultraviolet irradiation and N-methyl-N′-nitro-N-nitrosoguanidine treatment. Almost all resistant mutants excreted some l-lysine into the medium. Lysine excretion increased after repeated mutations. The mutant strain SR-V–1263 extracellularly produced about 2 mg/ml of l-lysine after shaking culture for 96 hr. The effect of various factors on lysine accumulation was investigated with strain SR-V–1263. The concentration of extracellular lysine reached a maximum (3.2 mg/ml) in medium containing 2% polypeptone under the experimental conditions.

Strain (injury)

10.1080/00021369.1979.10863536

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Citations (6)

Lysine production by S-(.BETA.-aminoethyl)-L-cysteine resistant mutants of Candida pelliculosa.

Agricultural and Biological Chemistry (1979)

Eiichi Takenouchi Tatsuo Yamamoto Donka K. Nikolova Hidehiko Tanaka Kenji Soda

S-(β-Aminoethyl)-L-cysteine (SAEC), a sulfur analog of L-lysine, significantly inhibited the growth of wild-type strains of Candida species. The growth inhibition of C. pelliculosa de-pended on SAEC concentrations, but L-lysine and L-α-aminoadipate restored growth effectively. SAEC-resistant mutants were induced from the wild-type strain of C. pelliculosa by ultraviolet irradiation and N-methyl-N'-nitro-N-nitrosoguanidine treatment. Almost all resistant mutants excreted some L-lysine into the medium. Lysine excretion increased after repeated mutations. The mutant strain SR-V-1263 extracellularly produced about 2mg/ml of L-lysine after shaking culture for 96 hr. The effect of various factors on lysine accumulation was investigated with strain SR-V-1263. The concentration of extracellular lysine reached a maximum (3.2 mg/ml) in medium containing 2% polypeptone under the experimental conditions.

Strain (injury)

10.1271/bbb1961.43.727

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Citations (5)

Excretion of lysine by lysine sulfur analogue resistant-mutants of Candida pelliculosa.

Agricultural and Biological Chemistry (1977)

Eiichi Takenouchi Donka K. Nikolova Kikuo Awano Kenji Soda Hidehiko Tanaka

10.1271/bbb1961.41.615

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Citations (2)

Lysine Biosynthesis in Yeasts

Bulletin of the Institute for Chemical Research, Kyoto University (1982)