N-methyl-N-nitrosourea is metabolised by mouse liver microsomes yielding highly reactive product(s) capable of alkylating cellular macromolecules. Based on cofactor requirement (NADPH, oxygen), inhibition (NaN3) and induction by phenobarbital, 3,4-benz(a)pyrene, and MNU, the reaction is cytochrome P-450-dependent. Analysis of the kinetics and dose dependence of alkylation in vivo and in tissue homogenates confirms the fact that MNU must undergo metabolic activation in vivo.

Nitrosourea

Phenobarbital

Metabolic intermediate

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Citations (1)

[Varying rate of destruction of alkylated molecules of RNA, protein and lipids in normal and neoplastic cells].

PubMed (1973)

Lerman Mi Abakumova OIu Kutsenko Ng Gorbachëva Lb Kukushkina Gv

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[Effect of N-alkyl-N-nitrosourea on the protein-synthesizing system of liver and hepatoma 22a cells].

PubMed (1973)

Abakumova OIu Ugarova TIu Pilipenko Nn Kutsenko Ng Lerman Mi

Nitrosourea

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[Reconstruction of biologically active ribosomes from protein-poor ribonucleoprotein particles and ribosomal protein].

PubMed (1966)

Spirin As Lerman Mi Gavrilova Lp Belitsina Nv

Ribosomal protein

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Citations (1)

[Kinetics of the in vivo synthesis of the polypeptide chain; the effect of translation inhibitors].

PubMed (1975)

Leĭtin Vl Lerman Mi

Effects of aurinetricarbonic acid and cycloheximide on kinetics of polypeptide chain synthesis and polyribosome protile in mouse liver cell in vivo are studied. Both compounds are found to decrease the absolute rate of protein synthesis and to increase the time of polypeptide synthesis. Cycloheximide changed the ratio of translating and non-translating ribosomes and different in size polyribosome types. Aurinetricarbonic acid exerts no effect on polyribosome profile. Effects of cycloheximide and aurinetricarbonic acid on kinetic parameters of translation and a mechanism of action of these compounds are studied.

Polysome

Polypeptide chain

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[Metabolism of N-methyl-N-nitrosourea in various mouse tissues under inhibition of protein and nucleic acid synthesis].

PubMed (1977)

Abakumova OIu Kutsenko Ng Lerman Mi

The contribution of C1-pool and antimetabolite utilization to modification (labeling) of macromolecules of various mouse tissues after a single injection of 1-14C-methylnitrosourea was studied, using inhibitors of protein and nucleic acid synthesis. It was shown that about 50% of radioactivity found in the proteins, lipoproteins and DNA (but not in RNA) might be supplied by the C1-pool and/or through utilization of radioactive metabolites in the first 3-6 hours following the injection of 1-14C-methylnitrosourea. The data obtained allow to evaluete the degree of alkylation of macromolecules in various organs and tissues.

Nitrosourea

Nucleic acid metabolism

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[Cytoplasmic precursors of polyribosomes].

PubMed (1971)

Leĭtin Vl Podobed Ov Lerman Mi

Polysome

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