Qunen Liu

China National Rice Research Institute

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Trends

Author Order

Document Type

Co-Authors

Liyong Cao

China National Rice Research Institute

Yingxin Zhang

China National Rice Research Institute

Weixun Wu

China National Rice Research Institute

Daibo Chen

China National Rice Research Institute

Xiaodeng Zhan

China National Rice Research Institute

Shihua Cheng

China National Rice Research Institute

Shihua Cheng

China National Rice Research Institute

Xihong Shen

China National Rice Research Institute

Ning Yu

Huzhou University

Beifang Wang

Liaoning Technical University

Cooperative Institutions

China National Rice Research Institute

Chinese Academy of Sciences

Chinese Academy of Agricultural Sciences

Rice Research Institute

Hunan Agricultural University

Zhejiang University

Rice University

University of Chinese Academy of Sciences

Huazhong Agricultural University

Ministry of Agriculture and Rural Affairs

Author Statistics

Papers

Citation

H-Index

i-10 index

Research Field

Fine mapping of the lesion mimic and early senescence 1 (lmes1) in rice (Oryza sativa)

Plant Physiology and Biochemistry (2014)

Zhi Li Ying-Xin Zhang Lin Liu Qunen Liu Zhenzhen Bi

Senescence

ORFS

10.1016/j.plaphy.2014.03.031

Cite

Citations (36)

Genetic mapping of a QTL controlling source–sink size and heading date in rice

Gene (2015)

Xiaodeng Zhan Bin Sun Zechuan Lin Zhiqiang Gao Ping Yu

Source size, sink size and heading date (HD) are three important classes of traits that determine the productivity of rice. In this study, a set of recombinant inbred lines (RILs) derived from the cross between an elite indica line Big Grain1 (BG1) and a japonica line Xiaolijing (XLJ) were used to map quantitative trait loci (QTLs) for source–sink size and heading date. Totally, thirty-one QTLs for source size, twenty-two for sink size, four for heading date and seven QTL clusters which included QTLs for multiple traits were identified in three environmental trials. Thirty QTLs could be consistently detected in at least two trials and generally located in the clusters. Using a set of BC4F2 lines, the QTL cluster in C5-1–C5-2 on chromosome 5 was validated to be a major QTL pleiotropically affecting heading date, source size (flag leaf area) and panicle type (neck length of panicle, primary branching number and the ratio of secondary branching number to primary branching number), and was narrowed down to a 309.52 Kb region. QTL clusters described above have a large effect on source–sink size and/or heading date, therefore they should be good resources to improve the adaptability and high yield potential of cultivars genetically.

Sink (geography)

10.1016/j.gene.2015.06.065

Cite

Citations (18)

Identification and mapping of an early leaf senescence trait, es2 mutant, in rice

Pakistan Journal of Botany (2020)

Sittipun Sinumporn Yuyu Chen Peipei Zhang Ying-Xin Zhang Liaqat Shah

Senescence

Identification

Trait

10.30848/pjb2020-6(36)

Cite

Citations (0)

ES5 is involved in the regulation of phosphatidylserine synthesis and impacts on early senescence in rice (Oryza sativa L.)

Plant Molecular Biology (2020)

Mohammad Hasanuzzaman Rani Qunen Liu Ning Yu Yingxin Zhang Beifang Wang

Abstract Leaf senescence, which affects plant growth and yield in rice, is an ideal target for crop improvement and remarkable advances have been made to identify the mechanism underlying this process. We have characterized an early senile mutant es5 ( e arly leaf s enescence 5 ) in rice exhibiting leaf yellowing phenotype after the 4-leaf stage. This phenotype was confirmed by the higher accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA), the disintegration of chloroplasts, reduction in chlorophyll content and photosynthetic rate and up-regulation of senescence-associated genes (SAGs) like Osh36 , OsI57 , and OsI85 . Positional cloning revealed that the es5 phenotype is the result of one base substitution in ES5 , encoding phosphatidylserine synthase (PSS) family protein, which is involved in the base-exchange type reaction to synthesize the minor membrane phospholipid phosphatidylserine. Functional complementation of ES5 in the es5 plants completely restored the wild-type phenotype. Ultra-high-performance liquid chromatography (UHPLC) analysis showed that es5 plants had increased levels of phosphatidylserine (PS) and decreased level of phosphatidylcholine (PC). These results provide evidence about the role of PS in rice leaf senescence.

Senescence

Wild type

10.1007/s11103-019-00961-4

Cite

Citations (14)

Impaired Function of the Calcium-Dependent Protein Kinase, OsCPK12, Leads to Early Senescence in Rice (Oryza sativa L.)

Frontiers in Plant Science (2019)

Beifang Wang Yingxin Zhang Zhenzhen Bi Qunen Liu Tingting Xu

Premature leaf senescence affects plant yield and quality, and numerous researches about it have been conducted until now. In this study, we identified an early senescent mutant es4 in rice (Oryza sativa L.); early senescence appeared approximately at 60 dps and became increasingly senescent with the growth of es4 mutant. We detected that content of reactive oxygen species (ROS) and malonaldehyde (MDA), as well as activity of superoxide dismutase (SOD) were elevated, while chlorophyll content, soluble protein content, activity of catalase (CAT), activity of peroxidase (POD) and photosynthetic rate were reduced in the es4 mutant leaves. We mapped es4 in a 33.5Kb physical distance on chromosome 4 by map-based cloning. Sequencing analysis in target interval indicated there was an eight bases deletion mutation in OsCPK12 which encoded a calcium-dependent protein kinase. Functional complementation of OsCPK12 in es4 completely restored the normal phenotype. We used CRISPR/Cas9 for targeted disruption of OsCPK12 in ZH8015 and all the mutants exhibited the premature senescence. All the results indicated that the phenotype of es4 was caused by the mutation of OsCPK12. Overexpression of OsCPK12 in ZH8015 enhanced the net photosynthetic rate (Pn) and chlorophyll content. OsCPK12 was mainly expressed in green organs. The results of qRT-PCR analysis showed that the expression levels of some key genes involved in senescence, chlorophyll biosynthesis, and photosynthesis were significantly altered in the es4 mutant. Our results demonstrate that the mutant of OsCPK12 triggers the premature leaf senescence; however, the overexpression of OsCPK12 may delay its growth period and provide the potentially positive effect on productivity in rice.

Senescence

10.3389/fpls.2019.00052

Cite

Citations (31)

A proteomic approach identifies novel proteins and metabolites for lesion mimic formation and disease resistance enhancement in rice

Plant Science (2019)

Zhiqiang Gao Qunen Liu Yingxin Zhang Hong Fang Yue Zhang

Metabolic pathway

10.1016/j.plantsci.2019.110182

Cite

Citations (10)

Modulation of rice grain shape and appearance by the GS10-encoded long coiled-coil protein

The Crop Journal (2024)

Yanmin Luo Yuyu Chen Ping Xu Beifang Wang Yiwei Kang

Modulation (music)

Coiled coil

10.1016/j.cj.2024.11.002

Cite

Citations (0)

LMM24 Encodes Receptor-Like Cytoplasmic Kinase 109, Which Regulates Cell Death and Defense Responses in Rice

International Journal of Molecular Sciences (2019)

Yue Zhang Qunen Liu Yingxin Zhang Yuyu Chen Ning Yu

Lesion mimic mutants are excellent models for research on molecular mechanisms of cell death and defense responses in rice. We identified a new rice lesion mimic mutant lmm24 from a mutant pool of indica rice cultivar “ZhongHui8015”. The LMM24 gene was identified by MutMap, and LMM24 was confirmed as a receptor-like cytoplasmic kinase 109 by amino acid sequence analysis. The lmm24 mutant displayed dark brown lesions in leaves and growth retardation that were not observed in wild-type ZH8015. The results of histochemical staining and TUNEL assays showed enhanced ROS accumulation and cell death in lmm24. Chloroplast degradation was observed in lmm24 leaves, with decreased expression of photosynthesis-related genes and increased expression of the senescence-induced STAYGREEN (SGR) gene and other senescence-associated genes. Furthermore, lmm24 exhibited enhanced resistance to rice blast fungus Magnaporthe oryzae (M. oryzae) and up-regulation of defense response genes. Our data demonstrate that LMM24 regulates cell death and defense responses in rice.

Senescence

Magnaporthe

Wild type

10.3390/ijms20133243

Cite

Citations (29)

OsCUL3a Negatively Regulates Cell Death and Immunity by Degrading OsNPR1 in Rice

The Plant Cell (2017)

Qunen Liu Yuese Ning Yingxin Zhang Ning Yu Chunde Zhao

Cullin3-based RING E3 ubiquitin ligases (CRL3), composed of Cullin3 (CUL3), RBX1, and BTB proteins, are involved in plant immunity, but the function of CUL3 in the process is largely unknown. Here, we show that rice (Oryza sativa) OsCUL3a is important for the regulation of cell death and immunity. The rice lesion mimic mutant oscul3a displays a significant increase in the accumulation of flg22- and chitin-induced reactive oxygen species, and in pathogenesis-related gene expression as well as resistance to Magnaporthe oryzae and Xanthomonas oryzae pv oryzae. We cloned the OsCUL3a gene via a map-based strategy and found that the lesion mimic phenotype of oscul3a is associated with the early termination of OsCUL3a protein. Interaction assays showed that OsCUL3a interacts with both OsRBX1a and OsRBX1b to form a multisubunit CRL in rice. Strikingly, OsCUL3a interacts with and degrades OsNPR1, which acts as a positive regulator of cell death in rice. Accumulation of OsNPR1 protein is greater in the oscul3a mutant than in the wild type. Furthermore, the oscul3a osnpr1 double mutant does not exhibit the lesion mimic phenotype of the oscul3a mutant. Our data demonstrate that OsCUL3a negatively regulates cell death and immunity by degrading OsNPR1 in rice.

Plant Immunity

10.1105/tpc.16.00650

Cite

Citations (122)

Genome-Wide Identification and Characterization of the PPPDE Gene Family in Rice

Agronomy (2024)

Wangmin Lian Xiaodeng Zhan Daibo Chen Weixun Wu Qunen Liu

Protein ubiquitination is common and crucial in cellular functions, however, little is known about how deubiquitinating enzymes (DUBs) reverse regulate the ubiquitination signaling process. PPPDE family proteins are a novel class of deubiquitinating peptidases with demonstrated deubiquitination/deSUMOylating activities. In this study, we identified 10 PPPDE genes from the rice (Oryza sativa L.) genome unevenly distributed on five chromosomes, where most of these members have not been reported to date. Based on the gene structure, the OsPPPDE family consists of three distinct subgroups within the phylogenetic tree. Cis-element analysis identified light/phytohormone-responsive, development, and abiotic stress-related elements in the promoters of OsPPPDE. Furthermore, we conducted and analyzed the transcript abundance of OsPPPDE under various tissues and stresses using the transcriptome data of 352 samples from the Rice Expression Database and GEO datasets. Moreover, OsPPPDE5 showed differential regulation of its transcript abundance during Cd and drought stress. Collinearity and syntenic analysis of 101 PPPDEs and PPPDE-like proteins in 10 plant genomes indicated that this family is evolutionarily conserved. Domestication analysis suggests that OsPPPDEs may contribute to indica–japonica divergence using the data from the 3K Rice Genome Project. Our study provides a foundation for further study on the function and molecular mechanism of the OsPPPDE gene family.

Identification

10.3390/agronomy14051035

Cite

Citations (0)