Junichi Koyatsu

Social Insurance Saitama Chuo Hospital

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Kazuhisa Ishi

Juntendo University Urayasu Hospital

Katsunari Kina

Juntendo University

Fujihiko Suzuki

Juntendo University Urayasu Hospital

Akira Saito

Saitama Medical University

Tetsuya Okazaki

Okayama University Hospital

Reiko KAZAMA

Institute of Clinical Research

Hiroshi Nakamura

The Nakamura Hajime Eastern Institute

Mathilde E. Boon

Leiden University

K Ishi

Juntendo University Urayasu Hospital

Tetsuya Okazaki

Institute of Clinical Research

Cooperative Institutions

Juntendo University Urayasu Hospital

Juntendo University

The University of Texas MD Anderson Cancer Center

The University of Tokyo

Massachusetts General Hospital

University of Massachusetts Boston

Osaka University

Institute of Clinical Research

Kyoto University

Cornell University

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[Changes in folate levels after cisplatin treatment of gastric cancer].

PubMed (1996)

Iba T Yuko Yagi Akio Kidokoro K Ishi Junichi Koyatsu

Currently, biochemical modulation for 5-fluorouracil (5-FU) by cisplatin (CDDP) is one of the most successful forms of chemotherapy for gastric cancer. Although the mechanism of this modulation is thought to increase intracellular folate levels, it is still unknown how much CDDP is needed to elevate folate levels. The purpose of this study is to determine the appropriate volume of CDDP as a modulator for 5-FU. Either 5, 20, 100 mg/body of CDDP was administered intravenously to advanced gastric cancer patients just before operation. Four hrs later, the stomach was resected and folate levels were measured in the tumor and normal mucosa by thymidylate synthase binding assay. The results showed folate elevation only after administration of 100 mg/body of CDDP, both in the tumor and mucosa (p < 0.01). In conclusion, if CDDP is infused as a bolus, a relatively large amount is needed to modulate the intratumor folate level.

Folate receptor

Bolus (digestion)

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Citations (1)

Letters to the Editors

Acta Cytologica (1999)

Uma Singhal Harsh Mohan Cristina A.T.S. Mitteldorf Antonio Carlos de Oliveira Misiara Iara Emiko de Carvalho

10.1159/000331317

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Citations (0)

[Quality Management System in Pathological Laboratory].

PubMed (2015)

Junichi Koyatsu Yoshihiko Ueda

Even compared to other clinical laboratories, the pathological laboratory conducts troublesome work, and many of the work processes are also manual. Therefore, the introduction of the systematic management of administration is necessary. It will be a shortcut to use existing standards such as ISO 15189 for this purpose. There is no standard specialized for the pathological laboratory, but it is considered to be important to a pathological laboratory in particular. 1. Safety nianagement of the personnel and environmental conditions. Comply with laws and regulations concerning the handling of hazardous materials. 2. Pre-examination processes. The laboratory shall have documented procedures for the proper collection and handling of primary samples. Developed and documented criteria for acceptance or rejection of samples are applied. 3. Examination processes. Selection, verification, and validation of the examination procedures. Devise a system that can constantly monitor the traceability of the sample. 4. Post-examination processes. Storage, retention, and disposal of clinical samples. 5. Release of results. When examination results fall within established alert or critical intervals, immediately notify the physicians. The important point is to recognize the needs of the client and be aware that pathological diagnoses are always "the final diagnoses".

Traceability

Medical laboratory

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Contents

Acta Cytologica (1998)

Gláucia Maria Machado‐Santelli Masuzo Kamiya Kunio Mizuguchi Makoto Yoshimoto Miyuki Tanaka

10.1159/000331813

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Citations (0)

Cytodiagnosis of Placental Site Trophoblastic Tumor

Acta Cytologica (1998)

Kazuhisa Ishi Fujihiko Suzuki Akira Saito Junichi Koyatsu Satoshi Akutsu

Placental site trophoblastic tumor (PSTT) is a rare form of trophoblastic neoplasm. Approximately 100 cases of PSTT have been reported, but we found no report on its cytodiagnosis.Case 1, a 39-year-old female, came to the hospital because of abnormal genital bleeding. Case 2, a 36-year-old female came because of amenorrhea for a year. In both cases, endometrial smear and intrauterine curettage suggested trophoblastic disease, and hysterectomy was performed. Laboratory data revealed a mild increase in human chorionic gonadotropin (hCG) and beta-hCG but normal human placental lactogen (hPL). In the cytologic examination, the background contained some hemorrhagic and fibrinous areas but no necrosis. Most tumor cells stained light green, were round or polygonal, and contained abundant cytoplasm. Some were palely stained and had vacuoles. Some cells showed hyperchromatism, an irregular nucleus, fine-to-coarse chromatin granules and markedly different sizes. Most of the cells were hPL positive, and a few were hCG positive.Both cases were considered benign because of rare mitoses despite cellular pleomorphism. However, careful follow-up is required. The differential diagnosis of PSTT is difficult from cytologic and biopsy specimens alone but may be achieved with additional magnetic resonance imaging findings and positive staining of hPL and hCG.

Human placental lactogen

Trophoblastic Tumor

Human chorionic gonadotropin

Gestational Trophoblastic Disease

10.1159/000331838

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Citations (12)

Central neurocytoma arising in right lateral ventricle-Cytological and pathological characteristics-

The Journal of the Japanese Society of Clinical Cytology (2004)

Tetsuya Okazaki Junichi Koyatsu Keiko Takeda Hiroshi Nakamura Hiroko Suto

背景:中枢性神経細胞腫central neurocytoma (以下, CN) は, 比較的新しい概念の腫瘍で細胞診の報告例はいまだ少ない. 今回われわれは, 右側脳室に発生したCNを経験したので, 術中の捺印および圧挫処理標本の細胞像を中心に報告する.症例:患者は28歳男性で, 頭部打撲にて救急外来を受診した. 頭部CTで右側脳室に石灰化を伴う腫瘍を認め, MRIでは腫瘍内部に嚢胞を伴っていた. 術中捺印細胞診標本で, 腫瘍細胞はライトグリーン淡染性の線維性物質を背景に, 散在性あるいは結合性の緩い細胞集塊としてみられ, 一部ではロゼット様の細胞配列を示していた. また, 核周囲が明るく抜けた細胞質を有する細胞境界明瞭な細胞も一部にみられた. 術中の組織所見では, エオジン淡染性の細胞質と均一な円形～類円形核を有する腫瘍細胞を認め, 細胞境界は不明瞭であった. 術中の細胞・組織所見と臨床所見からCNが疑われ, 術後の組織および免疫染色所見と電子顕微鏡的検索からCNと診断した.結論:CNの術中迅速診断において, 細胞診を併用することで, 凍結切片標本では捉えることのできない所見を得ることができた. これらの細胞学的所見に加え, 組織所見と臨床所見を考慮することによって, CNの術中迅速診断が可能になると思われた.

Central neurocytoma

10.5795/jjscc.43.380

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Immunocytochemical diagnosis of Bellini duct carcinoma.

PubMed (1997)

Tomio Arai Yasuyuki Kawachi K Ishi Junichi Koyatsu Katsunari Kina

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Cell transfer technique with Malinol mounting medium. An application to immunocytochemistory and ISH methods.

The Journal of the Japanese Society of Clinical Cytology (1996)

Ayako OHONO Katsunari Kina Tetsuya Okazaki Junichi Koyatsu Kazuhisa Ishi

10.5795/jjscc.35.657

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Citations (3)

Interlaboratory Comparison in HercepTest Assessment of HER2 Protein Status in Invasive Breast Carcinoma Fixed With Various Formalin-based Fixatives

Applied immunohistochemistry & molecular morphology (2003)

Kaoru Hashizume Yutaka Hatanaka Yuki Kamihara Tomoyuki Kato Sakae HATA

Although formalin-based fixatives are used in pathologic laboratories, there is no strictly standardized fixation protocol in Japan. To examine interlaboratory variation caused by different conditions of fixation in the assessment of human epidermal growth factor receptor (HER) 2 status on pathologic tissues, 274 archival invasive breast carcinomas from 5 different laboratories were evaluated using the HercepTest. In 1 laboratory in which 10% neutral buffered formalin was used, as recommended by the manufacturer, the overexpression rate was 22.4% and fell within the statistical expected range (20%–30%) for HER2 overexpression in breast carcinomas. The overexpression rates in the other 4 laboratories, in which either 20% nonbuffered formalin or 15% neutral buffered formalin was used, were near the expected range for HER2 overexpression. To clarify the influence of prolonged formalin fixation on the HercepTest, we compared 1-day with 7-day fixations using 36 cases fixed with 20% nonbuffered formalin. Of the 36 cases, 7 showed 3+ staining with 1-day fixation and sustained the same scoring results with 7-day fixation, although the staining intensities in these cases were reduced with the prolonged fixation. These results indicated that the immunohistochemical assessment of HER2 status with the HercepTest was comparatively resistant to prolonged fixation conditions and provided stable staining results in positive cases, particularly 3+ patients.

Breast carcinoma

10.1097/00129039-200312000-00011

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Citations (16)

A case of the mixed mesodermal tumor of the ovary.

The Journal of the Japanese Society of Clinical Cytology (1993)

Akira Saito Junichi Koyatsu Kazuhisa Ishi Sakae Utsuno Shu Saito

Mixed tumor

10.5795/jjscc.32.573

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Citations (0)