Yousef Mortazavi

Zanjan University of Medical Sciences

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Saeid Kaviani

Tarbiat Modares University

Samad Nadri

Zanjan University of Medical Sciences

Masoud Soleimani

Tarbiat Modares University

Mehrdad Noruzinia

Tarbiat Modares University

Masoud Soleimani

Tarbiat Modares University

Majid Farshdousti Hagh

Tabriz University of Medical Sciences

Najmaldin Saki

Ahvaz Jundishapur University of Medical Sciences

Ebrahim Nasiri

Mazandaran University of Medical Sciences

Mohammad Shahjahani

Ahvaz Jundishapur University of Medical Sciences

Saeideh Mazloomzadeh

Zanjan University of Medical Sciences

Cooperative Institutions

Zanjan University of Medical Sciences

Tehran University of Medical Sciences

Tarbiat Modares University

University of Tehran

Shahid Beheshti University of Medical Sciences

Pasteur Institute of Iran

Stem Cell Technology Research Center

Biotechnology Research Center

Iran University of Medical Sciences

Shariati Hospital

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In Silico Analysis for Determination and Validation of Human CD20 Antigen 3D Structure

International Journal of Peptide Research and Therapeutics (2017)

Zahra Payandeh Masoumeh Rajabibazl Yousef Mortazavi Azam Rahimpour

10.1007/s10989-017-9654-9

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Citations (3)

Archive of SID Th e survey of airways management equipments of emergency medical centers in Mazandaran Province

Yousef Mortazavi Ebrahim Nasiri H Babatabar Darzi Mahmoudi Hosein

A B S T R A C T Aims: In the critical life threatening situations, efforts of the emergency centers personnel are to eliminate the threatening factors, to keep airways open and save the victim life. To achieve these aims, proper equipments such as instruments for opening and keeping airways open are important. The aim of this study was to survey the status of equipment which are used for airway management of victims in critical situations by personnel of Mazandaran emergent teams. Methods: By using a descriptive study design, Mazandaran province divided into three geographic zones included West, East and Center zone. Three cities randomly chosen from each zone. Emergency centers of these selected cities visited research team members and by using of a checklist, all airways management equipments such as endotracheal tubes, airways, and Ambu bag, a flexible reservoir bag connected by tubing and a non-rebreathing valve to a face mask or endotracheal tube which used for artificial ventilation, surveyed. Results: Data analysis indicated, there is some deficiency in the airways management equipments. There were no infants endotracheal tubes with internal diameter size of 3.5-2.5 mm in the 30% of centers. In the 95% of the centers there were adult endotracheal tubes with internal diameter size of 6-7.5 mm. There were Ambu bag and oropharynx airways in the 86% and 84% of centers respectively. There weren't any nasopharynx airways. Only 84% of centers had proper endotracheal suction equipments. Conlusion: It is necessary to revise the list of equipment and also to provide new devices or/and repair some the airways management equipments of emergency centers.

Endotracheal tube

Personal Protective Equipment

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Changing the pattern of histone H3 methylation following treatment of erythroid progenitors derived from cord blood CD133+ cells with sodium butyrate and thalidomide

Ali Dehghani Fard Saeid Kaviani Mehrdad Noruzinia Masoud Soleimani Abbas Hajifathali

Background: Human β-like globin genes regulation during development from embyonic to adult stage results in generation of different types of hemoglobin with different functions. As β-thalassemia and sickle cell disease are disorders of β-globin chain, epigenetic drugs such as thalidomide and sodium butyrate which can induce γ-globin gene are considered as a novel therapeutic approach. Drugs effective in decreasing DNA methylation and alteration of histone methylation pattern can result in γ-globin gene upregulation. Materials and Methods: This study was performed on erythroid progenitors derived from cord blood CD133+ cells. Erythroid progenitors were treated with thalidomide and sodium butyrate as single and combination therapies in 10 μM concentrations. Chromatin Immuno Percipitation (ChIP) assay was used to evaluate the change in H3K27 methylation pattern. Also, Real-time PCR assay was used to compare the number of DNA fragments resulting from immunoprecipitation in different drug treatment groups. Results: Real-time PCR assay indicated considerable effect of thalidomide single therapy in decreasing H3K27 methylation compared with sodium butyrate and combination therapy. Conclusion: According to the results of this study, it seems that the synergistic effect of thalidomide and sodium butyrate combination therapy on γ-globin gene induction arises from other epigenetic mechanisms.

Sodium butyrate

Chromatin immunoprecipitation

Cord blood

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Ex Vivo Expansion of Umbilical Cord Blood Hematopoietic Stem Cells on Collagen- Fibronectin Coated Electrospun Nano Scaffold

Journal of Advances in Medical and Biomedical Research (2018)

Maryam Islami Yousef Mortazavi Masoud Soleimani Fatemeh Soleimanifar Samad Nadri

Surgical Outcome in Different Types of L4-L5 Degenerative Spondylolisthesis Based on CARDS Classification

Ex vivo

10.30699/jambs.30.142.4

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Citations (2)

N-RAS gene mutation in patients with aplastic anemia and aplastic anemia/ paroxysmal nocturnal hemoglobinuria during evolution to clonal disease

Blood (2000)

Yousef Mortazavi J. Adam Tooze E. C. Gordon‐Smith T. R. Rutherford

Paroxysmal nocturnal hemoglobinuria

Aplastic anemia

clone (Java method)

10.1182/blood.v95.2.646

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Citations (33)

Effect of Zoledronic Acid on Osteoblastic Differentiation of Mesenchymal Stem Cells and Epigenetic Changes

Journal of Analytical & Bioanalytical Techniques (2010)

Mehrdad Noruzinia Majid Frashdousti Hagh Yousef Mortazavi Masood Soleimani Saeed Kaviani

Z acid (ZA) is a bisphosphonate that is used to prevent skeletal fractures in patients with cancer, as well as for treating osteoporosis. Complete mechanism of action of ZA is not understood, and their actions on the osteoblasts are confusing. Epigenetic changes such as DNA methylation are regulatory mechanisms that control stem cell differentiation. In this situation, ZA induces osteoblastic differentiation in MSC, but regulatory mechanisms are unknown. Gene specific methylation of RUNX2, OSX, DLX5 and BSP genes and global methylation status were evaluated by methylation specific PCR (MSP) and specific antibody, respectively. Also, the effect of ZA on the BSP gene expression was evaluated by RT-PCR.

Zoledronic Acid

10.4172/2155-9872.1000054

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Increase of CXCR4 Expression on Expanded Non-enriched Cord Blood CD34+ Cells Using MSCs

DOAJ (DOAJ: Directory of Open Access Journals) (2005)

M Soleymani Hossein Mozdarani A A Pourfath Elah Yousef Mortazavi K Ali Moghadam

Received: 10/Jul/2004, Accepted: 27/Jul/2005 Introduction: A number of potential cell adhesion molecules, which mediate essential cell-to-cell or cell-to-matrix interactions, are expressed on the surface of CD34+ hematopoietic progenitor cells (HPCs), including integrins, CD44, and CXCR4. These molecules are essential for homing process. In this study, we compared the changes of expression of CD44 and CXCR4 on the CD34+ hematopoietic progenitor cells expanded on MSCs in the presence of cytokines. Material and Methods: Cord blood CD34+ cells were expanded using human bone marrow mesenchymal stem cells and cytokines (TPO, SCF, FLt-3, IL-6, and IL-3), and then expression of CD44 and CXCR4 on CD34+ cells were evaluated by flow cytometric analysis. Results: After 2 weeks of serum free culture of CD34+ cells in the presence of cytokines, the expression of CXCR4 on CD34+ cells was decreased 3.4 fold (p<0.05). In contrast, the expression of CXCR4 on CD34+ cells expanded on hMSCs was increased (p<0.05). The expression of CD44 on expanded CD34+ cells in both methods did not differ significantly. Conclusions: Our results indicated that co-culture of cord blood stem cells on hMSCs significantly increased CXCR4 expression on cord blood CD34+ cells.

Cord blood

Homing (biology)

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Role of Glutathione S-transferase (GSTM1, GSTT1) and CYP1A1 (cytochrome p450) Gene Polymorphisms in Susceptibility to Acute Myeloid Leukemia

Middle East journal of cancer (2020)

Yousef Mortazavi Robab Rahimi Fatemeh Azimi Shahrbano Rostami Minoosh Moghimi

Background: Acute myeloid leukemia (AML) may originate from the combination of genetic susceptibility factors and environmental exposure. The aim of this study was to investigate the association of GSTM1 and GSTT1 null genotypes and CYP1A1*2A allele with susceptibility to AML in an Iranian population. Method: In this case-control study, 200 patients with AML and 200 normal individuals as controls were included. GSTM1 and GSTT1 null genotypes were amplified using multiplex PCR and CYP1A1*2A polymorphisms were genotyped by PCR-RFLP. Result: The frequency of GSTM1 null genotype was significantly higher in the control group compared to the case group. The frequency of GSST1 null genotype was significantly lower in the controls. No association was observed between the studied CYP1A1*2A variant and the risk of acute myeloid leukemia. The combination of GSTT1 null genotype and CYP1A1 *2A AA and AC alleles further increased the risk of AML. Conclusion: GSTT1 null genotype can increase the risk of AML, particularly when combined with CYP1A1*2A allele. GSTM1 null genotype can also play a protective role and reduce the risk of AML. However, further studies are required on a larger number of patients.

Glutathione S-transferase

Null allele

Null (SQL)

Genotype frequency

10.30476/mejc.2019.78642.0

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Citations (4)

Domino Logic with an Efficient Variable Threshold Voltage Keeper

1993 IEEE International Symposium on Circuits and Systems (2005)

Amir Amirabadi Yousef Mortazavi Nariman Moezzi-Madani Ali Afzali‐Kusha Mehrdad Nourani

In this work, domino logic with a variable threshold voltage keeper which uses an efficient body bias is proposed. The generator which consists of a capacitor and a diode is based on the voltage doubler technique. In the proposed scheme, the keeper size may be increased to improve the noise-immunity of the domino logic without significantly increasing the power and the delay. In addition, the proposed generator circuit is simple, consumes a smaller area, and operates with a single supply voltage. The results of simulation for a 0.18 /spl mu/m CMOS technology show an improvement of 18% and 59% in power and delay, respectively, for this technique compared to the standard domino logic.

Domino logic

Voltage doubler

Domino

Logic level

10.1109/iscas.2005.1464927

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Citations (3)

Retinoic acid –loaded core-shell fibrous scaffold for neuronal differentiation of trabecular mesenchymal stem cells

DOAJ (DOAJ: Directory of Open Access Journals) (2021)

Khatereh Asadi Yousef Mortazavi Samad Nadri

Objective(s): Scientists believe that they can fabricate a biochemical scaffold and seed stem cells on it to create an extracellular matrix for tissue generation. This study sought to develop retinoic acid (RA)-loaded core-shell fibrous scaffolds (Poly-Caprolactone (PCL)/Polyethylene Oxide (PEO) based on electrospinning technique, to examine neural differentiation of trabecular mesenchymal stem cells (TM-MSCs). Materials and Methods: PEO-PCL core- shell fibrous scaffold was fabricated using coaxial electrospinning and Fourier transform infrared (FTIR) used to evaluate the chemical bond structure, scanning electron microscopy (SEM) has been utilized to evaluate surface topography and fibrous diameter, and transient electron microscopy (TEM) to evaluate core-shell structure. The neural differentiation was evaluated using Real-Time PCR.Results: The results of FTIR, SEM, and TEM confirm the fabrication of core-shell fibrous of PEO-PCL. The fabricated scaffold provides a suitable substrate for adhesion, cell proliferation, and differentiation. SEM images show changes in the morphology of TM-MSCs to neuronal cells. A sustained release of RA from the PEO/PCL scaffold was detected over 14 days. In addition, quantifying the expression of the gene indicates an increase in the gene expression of microtubule-associated protein 2 (MAP-2) gene.Conclusion:The PEO/PCL core-shell fibrous scaffold containing a RA constructed using coaxial electrospinning technique was a suitable substrate for inducing neuronal differentiation of TM-MSCs cultivated on core-shell scaffold.

10.22038/nmj.2021.08.08

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Citations (3)