Huajie Gu

Suzhou University of Science and Technology

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i-10 index

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Liling Hao

University of Shanghai for Science and Technology

Zhouping Wang

Jiangnan University

Nuo Duan

Jiangnan University

Shijia Wu

Jiangnan University

Yu Xia

Jiangnan University

Hua Ye

Zunyi Medical University

Haitao Wang

Chinese Academy of Fishery Sciences

Xiaoyuan Ma

Jiangnan University

Fei Xu

Shandong University

Youwen Ye

Yangpu Hospital of Tongji University

Cooperative Institutions

Suzhou University of Science and Technology

University of Shanghai for Science and Technology

Jiangnan University

State Key Laboratory of Food Science and Technology

Shanghai Jiao Tong University

Chinese Academy of Sciences

Central South University

Southeast University

Yangpu Hospital of Tongji University

Tsinghua University

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Oxidative stress and spermatogenesis suppression in the testis of cadmium-treated Bombyx mori larvae

Environmental Science and Pollution Research (2015)

Hongxia Yuan Fenjv Qin Weiqiang Guo Huajie Gu Aihua Shao

Malondialdehyde

Cadmium chloride

Glutathione reductase

Spermatozoon

10.1007/s11356-015-5818-3

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Citations (30)

Graphene oxide-assisted non-immobilized SELEX of okdaic acid aptamer and the analytical application of aptasensor

Scientific Reports (2016)

Huajie Gu Nuo Duan Shijia Wu Liling Hao Yu Xia

Abstract Okadaic acid (OA) is a low-molecular-weight marine toxin from shellfish that causes abdominal pain, vomiting and diarrhea, i.e., diarrheic shellfish poisoning. In this study, a ssDNA aptamer that specifically binds to OA with high affinity was obtained via Systematic Evolution of Ligands by Exponential Enrichment (SELEX) assisted by graphene oxide (GO). This aptamer was then applied to fabricate a novel direct competitive enzyme-linked aptamer assay (ELAA). At the optimized conditions, this ELAA method showed a low detection limit (LOD of 0.01 ng/mL), wide linear range (from 0.025 to 10 ng/mL), good recovery rate (92.86–103.34% in OA-spiked clam samples) and repeatability (RSD of 2.28–4.53%). The proposed method can be used to detect OA in seafood products with high sensitivity and can potentially be adapted for the determination of other small molecular analytes.

Aptamer

Linear range

Repeatability

SELEX Aptamer Technique

10.1038/srep21665

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Citations (100)

A novel aptasensor based on DNA hydrogel for sensitive visual detection of ochratoxin A

Microchimica Acta (2021)

Liling Hao XuTao Liu Sun‐Jie Xu Faliang An Huajie Gu

Aptamer

Absorbance

Rolling circle replication

Nanochemistry

10.1007/s00604-021-05000-y

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Citations (11)

A competitive fluorescent aptasensor for okadaic acid detection assisted by rolling circle amplification

Microchimica Acta (2017)

Huajie Gu Liling Hao Nuo Duan Shijia Wu Yu Xia

Aptamer

Rolling circle replication

Primer (cosmetics)

Deoxyribozyme

Linear range

Marine toxin

10.1007/s00604-017-2293-1

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Citations (27)

Fluorescence Resonance Energy Transfer Aptasensor of Ochratoxin A Constructed Based on Gold Nanorods and DNA Tetrahedrons

Journal of Agricultural and Food Chemistry (2022)

Liling Hao Mengqiu Li Kaimin Peng Tai Ye Xiuxiu Wu

Ochratoxin A (OTA) contamination of corn has received significant attention due to the wide distribution and high toxicity of OTA. The maximum residue limit standard of OTA in corn has been established by the Chinese Government and other unions. Nanoparticle-based fluorescence resonance energy transfer (FRET) assays are promising methods for the sensitive and fast detection of OTA. However, satisfactory detection sensitivity is commonly achieved with complicated signal amplification processes or specific nanoparticle morphologies, which means that these assays are not conducive to fast detection. This study proposes a simple and novel strategy to improve the sensitivity of FRET aptasensors. In this strategy, a DNA tetrahedron was first used in gold nanorod-based FRET aptasensors. DNA tetrahedron-modified gold nanorods are used as fluorescent acceptors, and Cy5-modified complementary sequences of the OTA aptamer are used as fluorescent donors. The aptamers of OTA are embedded in the DNA tetrahedrons, and FRET occurs when the aptamers hybridize with the Cy5-modified complementary sequences. The aptamer-integrated DNA tetrahedron modified on the surface of gold nanorods acts as an anchor, thus avoiding the crowding and entanglement of aptamers. Due to the competitive combination between the OTA aptamers and complementary sequences, the greater the amount of OTA, the less the amount of Cy5-modified complementary sequences that bind with the aptamers and the less the amount of Cy5 that is quenched. Thus, the fluorescence intensity is positively related to the OTA concentration. In this study, in the concentration range of 0.01-10 ng/mL, the fluorescence intensity was found to be linearly related to the logarithmic concentration of OTA. The limit of detection was calculated to be 0.005 ng/mL. The specificity of the developed biosensor was demonstrated to be efficient. The accuracy and stability of the developed aptasensor were also tested, and the method exhibited good performance in real samples.

Nanorod

Ochratoxins

10.1021/acs.jafc.2c03626

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Citations (18)

Development of Aptamer‐Based Non‐labeling Methods

Huajie Gu Liling Hao Zhouping Wang

This chapter focuses on the non-labeling methods, including surface plasmon resonance (SPR), quartz crystal microbalance (QCM), isothermal titration calorimetry (ITC), and microscale thermophoresis (MST). It then introduces their principles and applications in analytical detection and binding affinity. The non-labeling methods discussed have been widely used to determine molecular interactions. Therefore, they were originally employed to measure the binding affinity between aptamers and targets. The greatest advantage of the non-labeling method is that there is no need to label the aptamer, and thus it can avoid making any change of the aptamer. As is known, compared with only the aptamer itself, the change of aptamer may cause some variations in its secondary or tertiary structure. And some of the variations may have a serious effect on the affinity and specificity of the aptamer. Once suitable non-labeling detection instruments, even handheld equipment, are developed, the non-labeling method will be more suitable for real-time monitoring.

Aptamer

Microscale Thermophoresis

Isothermal Titration Calorimetry

Quartz Crystal Microbalance

Microscale chemistry

10.1002/9783527806799.ch11

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Citations (0)

A Review of the Methods for Detection of Staphylococcus aureus Enterotoxins

Toxins (2016)

Shijia Wu Nuo Duan Huajie Gu Liling Hao Hua Ye

Food safety has attracted extensive attention around the world, and food-borne diseases have become one of the major threats to health. Staphylococcus aureus is a major food-borne pathogen worldwide and a frequent contaminant of foodstuffs. Staphylococcal enterotoxins (SEs) produced by some S. aureus strains will lead to staphylococcal food poisoning (SFP) outbreaks. The most common symptoms caused by ingestion of SEs within food are nausea, vomiting, diarrhea and cramps. Children will suffer SFP by ingesting as little as 100 ng of SEs, and only a few micrograms of SEs are enough to cause SPF in vulnerable populations. Therefore, it is a great challenge and of urgent need to detect and identify SEs rapidly and accurately for governmental and non-governmental agencies, including the military, public health departments, and health care facilities. Herein, an overview of SE detection has been provided through a comprehensive literature survey.

Food poisoning

10.3390/toxins8070176

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Citations (150)

Optimization of hot water extraction of polysaccharides from Grifola frondosa by response surface methodology

Shipin Gongye Keji (2013)

Huajie Gu Yan Sun Liangzhi Li Wang Tao-yun Cuiying Hu

Grifola frondosa

Hot water extraction

Source

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Citations (0)

Metagenomic next-generation sequencing confirmed a case of sporadic human infection with Streptococcus suis in an urban area

The Journal of Infection in Developing Countries (2024)

Youwen Ye Huajie Gu Tianyu Li Xiaoyong Dai Bin Shi

Introduction: Streptococcus suis (S. suis) disease is a zoonotic infection caused by invasive S. suis and can lead to meningitis, septic shock, arthritis, and endocarditis. Early treatment is the key to reducing mortality. However, clinical manifestations of most cases are atypical, severely limiting rapid diagnosis and treatment. Case report: Here, we report a 74-year-old female patient diagnosed with S. suis infection. The main symptoms were hearing loss, lumbago, and scattered ecchymosis of the lower extremities and trunk. Blood non-specific infection indexes were significantly increased and platelets were significantly decreased; however, no pathogens were obtained from routine blood culture. Finally, the S. suis infection was confirmed by metagenomic next-generation sequencing (mNGS) of blood and cerebrospinal fluid. After antibiotic treatment, the limb and trunk scattered ecchymosis and lumbago symptoms were significantly relieved, but the hearing did not recover. Conclusions: Human infection with S. suis is rare in central cities, and it is easy to misdiagnose, especially in cases with atypical early symptoms. mNGS technology, combined with clinical observation, is helpful to clarify the direction of diagnosis and treatment, which is conducive to patient recovery.

Streptococcus suis

Ecchymosis

Blood Culture

10.3855/jidc.18805

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Citations (0)

Effects of La~(3+) on the Antioxidase Activity in Winter Wheat Seedlings under Low-temperature Stress

Anhui nongye kexue (2009)

Huajie Gu

[Objective] The study aimed to discuss the regulation action of La3+ in rare earth on the antioxidant enzyme activity of winter wheat seedlings under low-temperature stress.[Method] With the winter wheat variety Yongliang 4 as the tested material,5 concn.grads of La3+ at 5,10,20,30 and 40 mg/kg were set up to spray the wheat seedlings.The test method of simulating the low temperature chilling injury was used to study the regulatory effects of La3+ on SOD,POD and CAT activities in the leaves of winter wheat seedlings.[Result] In the 7 d of La3+ treatment and in the treatment with low temperature at 0,5 and 10 ℃,SOD activity in winter wheat was increased remarkably with the decrease of temperature and showed a trend of first rising and then falling with the La3+ treatment days.CAT and POD activities also showed a trend of first rising and then falling with the decrease of stress temperature and the increase of La3+ treatment days.After the winter wheat was treated with La3+ at 20 mg/kg under low-temperature stress,SOD,POD and CAT activities in the seedlings were higher than that in CK.[Conclusion] Treatment with suitable concn.(20 mg/kg) of La3+ could induce the winter wheat seedlings to produce the resistance against low-temperature stress,which was beneficial to increasing the cold hardiness in wheat.

Winter wheat

Hardiness (plants)

Falling Number

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