This study aimed to explore the regulatory effects and mechanisms of long noncoding RNA H19 (H19) on pulmonary injury, inflammation, and fibrosis of acute respiratory distress syndrome (ARDS).A rat model of ARDS was established by intratracheal instillation of 2 mg/kg lipopolysaccharide (LPS). qRT-PCR was performed to detect the expression of H19, miR-423-5p, tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, monocyte chemoattractant protein (MCP)-1, and vascular endothelial growth factor (VEGF). Histology score was assessed by hematoxylin-eosin (HE) staining. Enzyme-linked immunosorbent assay (ELISA) was used to detect the levels of proinflammatory cytokines and the content of VEGF in bronchoalveolar lavage fluid (BALF). The lung fibrosis was evaluated using western blot and Masson's trichrome staining. Dual-luciferase reporter gene assay was used for confirming the relationship between miR-423-5p and H19/FOXA1 in alveolar macrophage cells (MH-S) and alveolar epithelial cells (MLE-12). The regulatory effects of H19/miR-423-5p/FOXA1 axis on the inflammation and fibrosis were further analyzed in LPS-induced MH-S cells.The expression of H19 and FOXA1 was significantly up-regulated, while the expression of miR-423-5p was down-regulated in LPS-induced ARDS rats. Silencing of H19 decreased the mRNA expression of TNF-α, IL-1β, IL-6, MCP-1, and VEGF, the contents of TNF-α, IL-1β, IL-6, and VEGF in BALF, and histology score in LPS-induced ARDS rats. H19 knockdown also reduced the fibrosis scores and the protein expression of vimentin and α-SMA, and elevated the protein expression of E-cadherin in LPS-induced ARDS rats. Furthermore, silencing of miR-423-5p and overexpression of FOXA1 reversed the inhibitory effects of si-H19 on the inflammation and fibrosis of LPS-induced MH-S cells.Silencing of H19 relieved the pulmonary injury, inflammation and fibrosis of LPS-induced ARDS in rats. Silencing of H19 also alleviated the inflammation and fibrosis of LPS-induced MH-S cells through regulating the miR-423-5p/FOXA1 axis.
This research aims to explore the effect of l-arginine (Arg) upon lipopolysaccharide (LPS)-induced induction of the oxidative stress as well as subsequent apoptosis within ovine intestinal epithelial cells (IOECs). Through a 16 h incubation, cells were divided into four groups and the medium was replaced with different medium as follows: (1) control (Con), Arg-free Dulbecco's modified Eagle's F12 Ham medium (DMEM); (2) Arg treatment, Arg-free DMEM supplemented with 100 μM Arg; (3) LPS treatment, Arg-free DMEM supplemented with 10 μg/mL LPS; (4) LPS with Arg treatment, Arg-free DMEM supplemented with both 10 μg/mL LPS and 100 μM Arg. After culturing for 24 h in different mediums, some characteristics of cells in the four groups were measured. Addition of Arg increased cell viability induced with LPS compared with the LPS group (p < 0.05). Arg significantly decreased the release of dehydrogenase (LDH) and the production of malonaldehyde (MDA) (p < 0.05) within IOECs challenged by the LPS. Compared with the LPS group, cells treated with Arg and Arg + LPS increased (p < 0.05) mRNA as well as protein expression of glutathione peroxidase 1 (GPx1), catalase (CAT), superoxide dismutase 2 (SOD2), B-cell lymphoma 2 (Bcl2), quinone oxidoreductase 1 (NQO1), heme oxygenase (HO-1), and nuclear factor erythroid 2-related factor 2 (Nrf2). IOEC treatment with Arg reduced significantly (p < 0.05) apoptosis induced by the LPS (12.58 ± 0.79%). The results showed that Arg promoted the protein expression of Nrf2, up-regulated expression of the phase II metabolizing enzymes (NQO1 and HO-1), as well as antioxidative enzymes (GPx1, CAT, and SOD2) for alleviating oxidative injury and protected IOECs from LPS-induced apoptosis.
Based on the self-developed high pressure common rail diesel engine electronic control unit (ECU) and the rail pressure control model, the setting values control strategies for the target pressure step response of the engine under dynamic conditions were studied. The change rate and gradient of the target rail pressure were limited to track changes and change trend of target rail pressure which was acquired according to query ECU MAP. When there was a step response at the target pressure value due to the transient engine state, the value which acquired from the ECU MAP was not directly used as the target control value. Another way, the target rail pressure was approached step by step, and the step is gradually increased. The control strategy was validated on the engine test bench. Test results showed that the method can effectively filter out the mutation of target value caused by the external disturbance, and the overshoot of the rail pressure control, the time witch approached target MAP rail pressure value can also be reduced, and the response ability and stability of rail pressure controlling can be improved on dynamic engine state.
18 F-FDG PET/CT was performed to evaluate possible recurrent B-cell lymphoblastic lymphoma in a 34-year-old man. The images showed multiple foci of increased activity in the nerve root and peripheral nerve. A biopsy confirmed the diagnosis of neurolymphomatosis. After receiving chemotherapy, PET/CT showed progressive disease. The patient subsequently received the CD-19 chimeric antigen receptor T-cell therapy. A follow-up PET/CT acquired 30 days after chimeric antigen receptor T-cell therapy revealed no abnormal FDG activity.
Corn and wheat grains are two starch sources with considerably different ruminal digestion rates, which may lead to differing lipopolysaccharide (LPS) release in both rumen and hindgut affecting animal production. The objectives of this study were to (1) investigate the effects of different ruminal and faecal LPS concentrations induced by starch source (corn vs wheat) and starch concentrations (low vs high) on DMI, ruminal pH, ruminal fermentation patterns, milk production, and inflammatory responses; and (2) evaluate the possible translocation site of LPS in dairy goats. Eight lactating dairy goats with ruminal cannulas were used in a replicated 4 × 4 Latin square design with 2 × 2 factorial arrangement of treatments. Each experimental period consisted of 24 days long including 21 days for adaption and 3 days for data and sample collection. The four treatment diets were: corn and wheat grain combined with low (LS) and high grain starch (HS). Goats were fed equal amounts of a total mixed ration twice daily at 0700 hours and 1900 hours. Replacing corn with wheat in goat diet led to longer (P < 0.02) duration of ruminal pH <5.6, higher ruminal LPS (P < 0.05), but lower faecal LPS concentration. However, no differences between two grains in ruminal pH (mean, minimum and maximum), volatile fatty acids (VFA) and lactic acid concentration were observed. Goats fed HS diets had lower (P < 0.01) ruminal pH and higher (P < 0.01) ruminal concentrations of VFA and lactic acid, as well as higher (P < 0.01) ruminal and faecal LPS concentrations. Starch source did not affect DMI, milk yield and milk components whereas feeding HS versus LS diet had higher milk yield, lactose yield and improved milk efficiency (P < 0.05). Feeding wheat- versus corn-based diet showed only greater (P < 0.05) concentration of toll-like receptor-4, whereas feeding the HS versus LS diet consistently increased blood concentrations of amyloid A, haptoglobin, LPS binding protein, and LPS (P < 0.05). Analysis of Pearson correlation coefficients illustrated that the ruminal LPS concentration is more important than faecal LPS in inflammatory responses. In conclusion, replacing corn with wheat in lactating goat diet had negative impact on ruminal pH but little effects on fermentation characteristics and milk production. Increasing the dietary concentration of starch decreased ruminal pH status and thus increased risk of acidosis, whereas, feeding HS versus LS diets resulted in an improvement in milk yield, milk efficiency, and immunity response. Moreover, rumen acidosis induced by wheat based diet was accompanied with more severe inflammatory responses.
Long-term supplementation of a high-concentrate diet enhances the accumulation of lactate and decrease in pH in goat rumen, thereby disrupting the composition of microbial community. Studies have shown that incorporation of thiamine in high-concentrate diet increases ruminal pH and decreases rumen lactate concentration. To explore the effects of thiamine supplementation with a high-concentrate diet on alteration of the whole ruminal microbiota and their metabolites, 18 mid-lactating Saanen goats were randomly fed with one of three diets: (1) control diet (CON; n = 6; concentrate:forage 30:70), (2) high-concentrate diet (HG; n = 6; concentrate:forage 70:30), and (3) high-concentrate diet with 200 mg of thiamine/kg of DMI (HGT; n = 6; concentrate:forage 70:30). The goats received experimental diets for 8 weeks. Ruminal samples were collected on the last day of the 8 weeks for 16S rRNA gene sequencing and the liquid chromatograph–mass spectrometer (LC-MS) analysis. The results revealed significant alterations of the ruminal bacterial community structure and diversity in HGT groups compared to HG groups, with an overall dominance of Bacteroidetes at the phylum level and Oribacterium ( P < 0.05), Anaerobiospirillum ( P < 0.01), and Fibrobacter ( P < 0.01) at genus level in the HGT group. The LC-MS analysis revealed that thiamine supplementation resulted in lower levels of propionate ( P < 0.05), pyruvate ( P < 0.01), lactate ( P < 0.05), putrescine ( P < 0.05), tyramine ( P < 0.05), and histamine ( P < 0.01) and higher levels of acetate ( P < 0.05), succinates ( P < 0.01), oxaloacetic acid ( P < 0.01), leucine ( P < 0.01), valine ( P < 0.05), linoleic acid ( P < 0.05), docosahexaenoic acid ( P < 0.05), and 4-phenylbutyric acid ( P < 0.05) in the HGT group than in the HG group. The decrease in these compounds enhanced homeostasis in the rumen environment and suppressed epithelial inflammation. Correlation analysis revealed the potential relationships between ruminal metabolites and microbial community. These findings demonstrate that thiamine supplementation can alleviate subacute ruminal acidosis (SARA) by stabilizing the microbial community and reducing toxic unnatural compounds.
Ramie (Boehmeria nivea) is noted for the production of a large biomass that has a high protein content and is rich in antioxidants. It may thus serve as a high-quality forage material to replace alfalfa and improve the meat quality of farmed animals. In this study, we evaluated the carcass characteristics and meat quality of goats when 0, 35%, 75%, and 100% of dietary alfalfa was replaced with ramie. Crude protein content (linear, P < 0.0001) and key muscle color values at 24 h after slaughter decreased with increasing ramie levels. The content of most individual amino acids, non-essential amino acids (NEAA), total amino acids (TAA), branched chain amino acids (BCAA), functional amino acids (FAA), and flavor amino acids (DAA) decreased (P < 0.05) with increasing dietary ramie. The diet in which 35% of alfalfa was replaced with ramie yielded meat with the highest amino acid content, whereas the fatty acid profile was unaffected by the inclusion of ramie. These results indicate that ramie could be used as a potential dietary forage resource for goats, and that substituting 35% of alfalfa with ramie, which is equivalent to 126 g/kg DM content, would be optimal in terms of goat meat quality.
In the transportation of natural gas hydrate slurry, problems such as blockage and sedimentation caused by hydrates aggregation are often encountered. In addition, the risk control of hydrate decomposition is particularly important. Therefore, in order to investigate the flow characteristics and morphology of hydrate slurring, the phase equilibrium model of natural gas hydrate in water-based drilling fluid system is established. It is found that substances such as sodium chloride, potassium chloride, and glycerol all cause a decrease in the temperature at which hydrates are formed. Then, the temperature and pressure distribution in annulus tube are calculated based on the mixed flow model. The results show that the NGS through an annulus could be decomposed under most conditions involved in this paper. The higher the volume concentration of the NGS, the lower the temperature in annulus tube. Because the greater the hydrate concentration, the more hydration decomposition along the process, and the decomposition of the hydrate is endothermic, so the decomposition of the hydrate will make the temperature in annulus tube lower. Under the condition that the hydrate concentration is 10% and the inlet flow rate of the drilling fluid is 160 L/s, the inlet temperature of the drilling fluid has a great influence on the temperature in the ring air pipe. When the inlet temperature is relatively low, it is in the stable state of hydrate at the bottom of well and above part of the area. Therefore, controlling the temperature of drilling fluid entry would be a better measure to control the amount of hydrate decomposition.
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