Siv Johnsen‐Soriano

Universidad Europea de Madrid

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Emma Arnal

Universidad Europea de Madrid

Francisco J. Romero

Generalitat Valenciana

Francisco Bosch‐Morell

Universidad Cardenal Herrera CEU

María Miranda

Universidad Cardenal Herrera CEU

María Sancho‐Tello

INCLIVA Health Research Institute

Amparo Návea

Institut Català de Retina

M. Díaz-Llopis

Hospital Universitari i Politècnic La Fe

María Muriach

Universitat Jaume I

Jorge M. Barcia

Valencia Catholic University Saint Vincent Martyr

Cristina Peris‐Martínez

Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana

Cooperative Institutions

Universitat de València

Oslo University Hospital

University of Oslo

Universidad Cardenal Herrera CEU

Norwegian Cancer Society

Valencia Catholic University Saint Vincent Martyr

Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana

Johannes Gutenberg University Mainz

Harvard University

Hospital Universitari i Politècnic La Fe

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DHA administration protects antioxidant defenses in human retinal pigmented epithelial cells in high concentration glucose

Acta Ophthalmologica (2010)

Emma Arnal Siv Johnsen‐Soriano FJ ROMERO

Purpose Diabetic retinopathy is a leading cause of visual loss and increased nitric oxide (NO) has been correlated with that. The aim of this study was to evaluate protective properties of Docosahexaenoic acid (DHA) at retinal pigment epithelial (RPE) cells exposed to high glucose concentration. Methods ARPE-19 were cultured 4 days with normal blood glucose concentration (5,5 mM D-glucose), followed by exposure to either normal (5,5 mM) or high (45 mM) concentration of D-glucose during the next 5 days and DHA was added to the culture medium. Four different study groups were obtained: control group (5,5mM D-glucose) (C), control+DHA group(5,5 mM D-glucose+6,7 uM DHA)(CDHA), glucose group (45 mM D-glucose)(G) and glucose+DHA group (45 mM D-glucose+ 6,7 uM DHA)(GDHA). At the end of the experiment cells were washed, sonicated and lysed and the homogenates criopreserved. The total antioxidant capacity was measured with a commercial kit (Antioxidant Assay Kit, Cayman) and to measured the total nitrites a commercial kit was used (Parameter, Total Nitrite, R&D Systems). Results Antioxidant capacity in Ggroup was decreased significantly (0.97±0.10 mM/mg prot) when compared with the Cgroup (1.52±0.09 mM/mg prot). DHA administration reversed this alteration to normal values in the GDHA group (1.41 ± 0.16 mM/mg prot, p< 0.05 vs control). The total nitrites were significantly elevated in Ggroup (1.09±0.35 umol/L/mg prot) when compared to the Cgroup (0.53±0.08 umol/L/mg prot) and DHA administration reversed this alteration to normal values (0.49±0.12 umol/L/mg prot, p< 0.05 vs G). Conclusion DHA might be useful in protecting diabetic retina, though further studies are needed to better understand this mechanisms.

10.1111/j.1755-3768.2010.378.x

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The Anti-Oxidative Component of Docosahexaenoic Acid (DHA) in the Brain in Diabetes

Elsevier eBooks (2013)

Emma Arnal María Miranda Siv Johnsen‐Soriano Francisco J. Romero

10.1016/b978-0-12-405885-9.00013-9

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Citations (2)

Role of oxygen and nitrogen species in experimental uveitis: anti-inflammatory activity of the synthetic antioxidant ebselen

Free Radical Biology and Medicine (2002)

Francisco Bosch‐Morell Joaquı́n Romá Nuria Marı́n Belén Romero Antonio Rodriguez-Galietero

Ebselen

Malondialdehyde

10.1016/s0891-5849(02)00954-1

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Oxidative stress in corneal limbal rings

Acta Ophthalmologica (2010)

Siv Johnsen‐Soriano Emma Arnal FJ ROMERO

Purpose The aim of this work was to study oxidative stress markers in corneal limbal rings stored in Optisol at different preservation times. Methods Total Antioxidant capacity was measured with a commercial kit (Antioxidant Assay Kit, Cayman). Total nitrites were measured with a commercial kit where the amount of total nitrites was monitored spectofotometrically by reading the absorbance at 540 nm. Moreover, HNE (4-hydroxy-2-nonenal) immunohistoquemistry was performed at 3 µm thick paraffin sections. Results The antioxidant capacity in corneal limbal rings preserved for 7 days were decreased significantly (0.5 ± 0.02 mM/mg prot) when compared to a fresh corneal limbal ring (1.1 ± 0.28 mM/mg prot) * p< 0.05. Moreover, the total nitrites were significantly elevated in corneal limbal rings preserved for 7 days (1.47 ± 0.18 umol/L/mg prot) when compared to fresh corneal limbal rings ( 0.89 ± 0.46 umol/L/mg prot) * p< 0.05. Immunohistoquemistry revealed that there exist an increase in HNE positive cells as conservation time increases. Conclusion Oxidative stress factors increases with time in corneal limbal rings preserved in Optisol.

10.1111/j.1755-3768.2010.314.x

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Transient Bevacizumab (Avastin)-Induced Alterations in Rat Eyes

Ophthalmic Research (2008)

María Sancho‐Tello Siv Johnsen‐Soriano María Muriach Francisco Bosch‐Morell Manuel Díaz-Llopis

To study the histopathological, biochemical and functional effects of intravitreal bevacizumab on the rat eye, with special emphasis on its immediate pro-inflammatory features eventually associated with cellular oxidative burden.Histopathological evaluation was performed 24 h, 1 and 4 weeks after bevacizumab (75 microg/rat eye) or saline intravitreal injection, as well as biochemical analysis of oxidative stress-related markers and electroretinograms.Bevacizumab induces a transient inflammatory reaction together with a modification of the b-wave amplitude and latency of the electroretinogram. No changes were observed in any of the oxidative stress markers studied at any time after injection.Intravitreal bevacizumab injection per se generates an immediate, transient and mild inflammation of the rat eye, which is not associated with oxidative stress in ocular tissues.

Intravitreal administration

10.1159/000162263

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DHA restores VEGF via Nrf2 protection pathway inhibiting oxidative damage in RPE at high glucose levels

Investigative Ophthalmology & Visual Science (2013)

Siv Johnsen‐Soriano Emma Arnal Miguel Flores‐Bellver Luis Bonet‐Ponce Francisco J. Romero

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Changes In The Expression Of Genes Related To Oxidative Stress In Rd1 Mice.

Investigative Ophthalmology & Visual Science (2012)

Violeta Sánchez-Vallejo Miguel Flores‐Bellver Raquel Álvarez-Nölting Siv Johnsen‐Soriano María Miranda

Expression (computer science)

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Benefitial Effects of Docosahexaenoic Acid in Experimental Diabetic Retinopathy

Investigative Ophthalmology & Visual Science (2009)

Javier Araiz Emma Arnal María Miranda Siv Johnsen‐Soriano M. Díaz-Llopis

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Ebselen Prevents Chronic Alcohol‐Induced Rat Hippocampal Stress and Functional Impairment

Alcoholism Clinical and Experimental Research (2007)

Siv Johnsen‐Soriano Francisco Bosch‐Morell María Miranda Samuel Asensio Jorge M. Barcia

Background: Most of the previously published data suggest a role for oxidative or nitrosative stress in ethanol‐induced nervous system damage. Moreover, ethanol is able to impair learning abilities in adult mammalian brain, a process suggested to be directly related to hippocampal neurogenesis. Ebselen, a synthetic compound with antioxidant properties, is able to prevent ethanol‐induced impairment of neurogenesis in adult rats. The aim of the present work was to further demonstrate the ability of ebselen to prevent biochemical alterations, and preserve long‐term potentiation (LTP) and learning abilities, in the hippocampus of chronic alcoholic adult rats. Methods: Biochemical markers of oxidative stress (glutathione and malondialdehyde) were assayed in hippocampi of control rats and animals fed a liquid alcoholic diet (Lieber‐De Carli) supplemented or not with ebselen. Long‐term potentiation and hippocampal‐dependent tests were studied in all animal groups. Results: The hippocampal concentrations of glutathione and malondialdehyde were decreased and increased, respectively, in alcohol‐treated animals, and did not differ from those of the control and the alcohol+ebselen groups. Long‐term potentiation in hippocampal slices from ethanol‐treated animals was prevented, when compared with controls, and occurred with a similar profile in control animals and in the alcohol+ebselen groups. Learning ability was tested with the Morris water maze test. Escape latencies were higher in ethanol‐treated rats than in control animals or the ones treated with ethanol+ebselen. Conclusions: The results herein strongly suggest that oxidative mechanisms may underlie the hippocampal effects of ethanol in adult rats, in view of the protective effect of ebselen.

Ebselen

Malondialdehyde

10.1111/j.1530-0277.2006.00329.x

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Citations (42)

IL-2 and IFN-gamma in the retina of diabetic rats

Graefe s Archive for Clinical and Experimental Ophthalmology (2010)

Siv Johnsen‐Soriano María Sancho‐Tello Emma Arnal Amparo Návea Enrique Cervera

Nitrotyrosine

Proinflammatory cytokine

10.1007/s00417-009-1289-x

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Citations (35)