Kar-Yee Yu

Chang Gung University

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Peng-Nien Huang

Chang Gung University

Guang‐Wu Chen

Chang Gung University

Yueh-Te Lin

Chang Gung Memorial Hospital

Sheng‐Yu Huang

First Affiliated Hospital of Jiangxi Medical College

Shin‐Ru Shih

Chang Gung University

Kuan‐Ting Liu

National Sun Yat-sen University

Chung‐Guei Huang

Chang Gung University

Yu-An Kung

Chang Gung University

Shu‐Li Yang

Linkou Chang Gung Memorial Hospital

Chia-Pei Chen

Linkou Chang Gung Memorial Hospital

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Chang Gung University

Linkou Chang Gung Memorial Hospital

Chang Gung Memorial Hospital

Icahn School of Medicine at Mount Sinai

University of Hong Kong

National Cheng Kung University

National Yang Ming Chiao Tung University

Memorial Hospital of South Bend

Kaohsiung Medical University

Kaohsiung Chang Gung Memorial Hospital

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Antibody titers measured by commercial assays are correlated with neutralizing antibody titers calibrated by international standards

medRxiv (Cold Spring Harbor Laboratory) (2021)

Yu-An Kung Chung‐Guei Huang Sheng‐Yu Huang Kuan‐Ting Liu Peng-Nien Huang

Abstract The World Health Organization (WHO) has highlighted the importance of an international standard (IS) for SARS-CoV-2 neutralizing antibody titer detection, with the aim of calibrating different diagnostic techniques. In this study, IS was applied to calibrate neutralizing antibody titers (IU/mL) and binding antibody titers (BAU/mL) in response to SARS-CoV-2 vaccines. Serum samples were collected from participants receiving the Moderna (n = 20) and Pfizer (n = 20) vaccines at three time points: pre-vaccination, after one dose, and after two doses. We obtained geometric mean titers of 1404.16 and 928.75 IU/mL for neutralizing antibodies after two doses of the Moderna and Pfizer vaccines, respectively. These values provide an important baseline for vaccine development and the implementation of non-inferiority trials. We also compared three commercially available kits from Roche, Abbott, and MeDiPro for the detection of COVID-19 antibodies based on binding affinity to S1 and/or RBD. Our results demonstrated that antibody titers measured by commercial assays are highly correlated with neutralizing antibody titers calibrated by IS.

Antibody titer

Antibody response

10.1101/2021.07.16.21260618

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Factors influencing neutralizing antibody titers elicited by coronavirus disease 2019 vaccines

Microbes and Infection (2022)

Yu-An Kung Sheng‐Yu Huang Chung‐Guei Huang Kuan‐Ting Liu Peng-Nien Huang

Antibody titer

Coronavirus

10.1016/j.micinf.2022.105044

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Quantifying Neutralizing Antibodies in Patients with COVID-19 by a Two-Variable Generalized Additive Model

mSphere (2022)

Kuan‐Ting Liu Yu‐Nong Gong Chung‐Guei Huang Peng-Nien Huang Kar-Yee Yu

Herein, we present a new approach for serological testing for SARS-CoV-2 antibodies using innovative laboratory methods that demonstrate a combination of biology and mathematics. The traditional virus neutralization test is the gold standard method; however, it is time-consuming and poses a risk to medical personnel.

Gold standard (test)

2019-20 coronavirus outbreak

10.1128/msphere.00883-21

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Citations (16)