Brigitte M. N. van de Lang-Born

Erasmus MC

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Joop S.E. Laven

Erasmus MC

Bhanu Kalra

Abbott Fund

EAP Steegers

Erasmus MC

Mohamed Fareez Meerasahib

Oxford Brookes University

Ajay Kumar

Indian Institute of Technology Bombay

Marlies E. Kevenaar

Franciscus Vlietland

Sarah Schalekamp‐Timmermans

Erasmus MC

Ramon Dykgraaf

Erasmus University Rotterdam

Axel P. N. Themmen

Erasmus MC

Gopal Savjani

Medical Diagnostic Laboratories (United States)

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Erasmus MC

Erasmus University Rotterdam

Rotterdam University of Applied Sciences

The University of Texas MD Anderson Cancer Center

University Medical Center

Abbott Fund

Medical Diagnostic Laboratories (United States)

Oxford Brookes University

Massachusetts General Hospital

Columbia University

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Serum Anti-Müllerian Hormone Levels Reflect the Size of the Primordial Follicle Pool in Mice

Endocrinology (2006)

Marlies E. Kevenaar Mohamed Fareez Meerasahib Piet Kramer Brigitte M. N. van de Lang-Born Frank H. de Jong

Reproductive aging is the decline of female fertility with age. It is caused by the decrease in the number of growing follicles, resulting from primordial follicle pool depletion. Recently, we have shown that anti-Müllerian hormone (AMH) is produced by growing follicles, and studies in women indicate that serum AMH levels decrease with age and correlate with antral follicle count. However, whether serum AMH levels correlate directly with the size of the primordial follicle pool cannot be determined in women. In this work, we describe studies in mice in which we determined the dynamics of ovarian follicles during aging. Furthermore, we describe the development of a mouse AMH ELISA, allowing us to measure AMH levels in mice, for the first time. We observed that serum AMH levels decline with increasing age, whereas expression of AMH in individual growing follicles, studied by immunohistochemistry, did not change with age. Thus, the decline in serum AMH correlates directly with the decline in the number of growing follicles (r = 0.86, P < 0.0001). We observed that the number of growing follicles correlated with the number of primordial follicles (r = 0.93, P < 0.0001). Similarly, we found a strong correlation between AMH levels and number of primordial follicles (r = 0.83, P < 0.0001). In conclusion, serum AMH levels reflect the size of the primordial follicle pool in aging mice. Therefore, AMH is an excellent marker to assess the quantitative aspect of ovarian reserve, which may be useful for women at risk for early ovarian aging such as survivors of childhood cancers.

Anti-Müllerian hormone

Antral follicle

Ovarian Reserve

10.1210/en.2005-1588

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Reference ranges of anti‐Müllerian hormone and interaction with placental biomarkers in early pregnancy: the Generation R Study, a population-based prospective cohort study

Endocrine Connections (2022)

Ramon Dykgraaf Sarah Schalekamp‐Timmermans Maria C. Adank Sjoerd A.A. van den Berg Brigitte M. N. van de Lang-Born

The primary objective of this study is to establish maternal reference values of anti-Müllerian hormone (AMH) in a fertile multi-ethnic urban pregnant population and to evaluate the effect of gestational age. The secondary objective of this study is to explore the association between AMH and placental biomarkers.This study was embedded in the Generation R Study, an ongoing population-based prospective cohort study from early pregnancy onwards.City of Rotterdam, the Netherlands, out of hospital setting.In 5806 women, serum AMH levels were determined in early pregnancy (median 13.5 weeks; 95% range 10.5-17.2).None.Maternal AMH levels in early pregnancy and its association with placental biomarkers, including human chorionic gonadotrophin (hCG), soluble fms-like tyrosine kinase-1 (sFLT), and placental growth factor (PLGF).A nomogram of AMH in early pregnancy was developed. Serum AMH levels showed a decline with advancing gestational age. Higher AMH levels were associated with a higher level of the placental biomarkers hCG and sFLT in early pregnancy. This last association was predominantly mediated by hCG. AMH levels were negatively associated with PLGF levels.In this large study, we show that AMH levels in early pregnancy decrease with advancing gestational age. The association between AMH and the placental biomarkers hCG, sFLT, and PLGF suggests a better placental development with lower vascular resistance in mothers with higher AMH levels. Hence, AMH might be useful in predicting adverse pregnancy outcomes due to impaired placental development.

Anti-Müllerian hormone

Generation R

Human chorionic gonadotropin

10.1530/ec-22-0320

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Citations (3)