E G Semenova

Russian Academy of Sciences

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V.P. Paribok

Institute of Cytology

Alex Bulychev

Moderna Therapeutics (United States)

Alexey Yu. Semenov

Semenov Institute of Chemical Physics

I. B. Nemecek

Lomonosov Moscow State University

Inna I. Severina

Lomonosov Moscow State University

A V Kargapolov

South Ural State University

L. V. Konevega

Russian Academy of Sciences

N. Ya. Gil’yano

Petersburg Nuclear Physics Institute

S V Kartsova

G G Polianskaia

Institute of Cytology

Cooperative Institutions

Lomonosov Moscow State University

Moscow State University

Russian Academy of Sciences

Institute for Information Transmission Problems

BioEnergetics (United States)

Institute of Cytology

Physico-Technical Institute

Petersburg Nuclear Physics Institute

Moderna Therapeutics (United States)

Takeda (United States)

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ON THE STIMULATION OF DNA SYNTHESIS UNDER X-IRRADIATION OF HeLa CELLS.

V.P. Paribok E G Semenova

HeLa

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[Unscheduled DNA synthesis and repair of HeLa Zh-63 cells sublethally damaged by irradiation].

PubMed (1970)

V.P. Paribok E G Semenova

HeLa

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[The phospholipid composition of the adenosine triphosphatase complex from bull heart mitochondria].

PubMed (1975)

A V Kargapolov S V Kartsova E G Semenova Mikel'saar Kh

Adenosine triphosphatase

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[Effect of the segregation of neutral red, acridine orange and ammonium chloride by L cells (subline LSM) on lysosomal hydrolase activity].

PubMed (1986)

Alex Bulychev E G Semenova O A Assinovskaia

Kinetics of Neutral red (NR) and Acridine orange (AO) uptake by cultured L cells (subline LSM) has been studied. It was found that the uptake of both NR and AO, with their constant concentrations in the medium was characterized as a two-phase process. During 2 hours, these cells concentrated as much as 90% of the total amount of NR and AO taken up during the whole incubation period. The segregation and accumulation of NR, AO as well as NH4Cl took place in lysosomes. NR and AO concentrations within the cells exceed by 600 and 400 times, respectively, those in the medium. NR, AO and NH4+ accumulation in cells resulted in inhibition of the activity of the following lysosomal hydrolases: cathepsins B and D, acid lipase, N-acetyl-beta,D-glucosaminidase, beta-galactosidase, acid phosphatase and galactosyltransferase, the latter being a marker of Golgi apparatus. The effect of lysosomal enzyme activity inhibition on the cell economy, and a possible role of lysosomotropic agents as regulators of the lysosomal apparatus functional activity are discussed.

Acridine orange

Ammonium chloride

Neutral red

Galactosyltransferase

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Characteristics of spontaneously transformed human endothelial ECV304 cell line: II. Functional responses of ECV304 cells

Cell and Tissue Biology (2009)

N. Ya. Gil’yano E G Semenova R. F. Fedortseva L. V. Konevega

Thymidine

10.1134/s1990519x09030092

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Reconstitution of biological molecular generators of electric current. H+-ATPase.

Journal of Biological Chemistry (1976)

Л.А. Драчев A. Jasaitis H.M. Mikelsaar I. B. Nemecek Alexey Yu. Semenov

1. Generation of a transmembrane electric potential difference by oligomycin-sensitive ATPase complex, incorporated into spherical or planar phospholipid membrane, has been demonstrated. To this end, penetrating anion probe and direct voltmeter measurement of electric potential across phospholipid membrane were used. It was found that ATP-induced electric response is sensitive to oligomycin and protonophorous uncouplers. 2. The effect of variations in the phospholipid component of proteoliposomes on the electric generation was studied. It was revealed that the usage of mitochondrial phospholipids and phosphatidylethanolamine allows the highest values of membrane potential to be obtained in the case of ATPase proteoliposomes. In the case of cytochrome oxidase and bacteriorhodopsin proteoliposomes, phosphatidylserine was also shown to be quite suitable. Phosphatidylcholine was absolutely ineffective in all cases. 3. In proteoliposomes, containing both ATPase and bacteriorhodopsin, ATP and light induced generation of the electric field of the same direction. 4. In ATPase + cytochrome oxidase proteoliposomes, ATP hydrolysis and ascorbate oxidation was found to support electric generation of the same direction if cytochrome c was inside vesicles. Oxidation via external cytochrome c resulted in formation of electric field of the direction, opposite to that induced by ATP hydrolysis. 5. The data obtained in experiments with proteoliposomes of different types are discussed. The conclusion is made that conversion of energy of different resources into electric form is a common feature of membraneous energy transducers, which is in agreement with the Mitchellian principle of cellular energetics.

Oligomycin

Phosphatidylethanolamine

F-ATPase

10.1016/s0021-9258(17)32943-5

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Citations (44)

[The effect of cryopreservation on the cytogenetic characteristics of a cell subline of skin fibroblasts from the Indian muntjac].

PubMed (1990)

G G Polianskaia E G Semenova N. A. Shubin

After thawing cells, previously cryopreserved in the presence of dimethyl sulfoxide (DMSO), a decrease in their viability and increase in unscheduled DNA synthesis was observed. In 7 days, these parameters restored to the control level. Cryopreservation without DMSO resulted in the decrease in both cell viability and replicative and unscheduled DNA synthesis. In 14 days, these characteristics were seen to return to the normal level. Cryopreservation of cells without DMSO and their preservation in liquid nitrogen induced the frequency of chromosomal aberrations, mostly chromosomal breaks. The frequency of chromosomal aberrations increased with the duration of cell preservation in liquid nitrogen. The normal level was achieved following 7 days after cell thawing. Cells treated with DMSO only (without cryopreservation) display an increased number of chromosomal and chromatid breaks and translocations. Nonrandom distribution of chromosomal aberrations was observed, with particular chromosomes being involved in the appearance of dicentrics and translocations. The data obtained indicate that cryoprotective activity of DMSO is probably associated with the cell repair systems. The detected antimutagenic and mutagenic activity of DMSO may presumably reflect various conditions for its interaction with cells (with or without cryopreservation), as well as it may be specific for the muntjac cell line used in the present work.

Viability assay

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Citations (1)