David F. Lewis

Louisiana State University Health Sciences Center Shreveport

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Yuping Wang

Louisiana State University Health Sciences Center Shreveport

Yang Gu

Louisiana State University Health Sciences Center Shreveport

Brian G. Lake

University of Surrey

Costas Ioannides

University of Surrey

Howard M. Colquhoun

University of Reading

Peter S. Goldfarb

University of Surrey

Maurice Dickins

Simcyp (United Kingdom)

Thomas J. Garite

VPDiagnostics (United States)

Dennis V. Parke

University of Surrey

D. V. Parke

University of Surrey

Cooperative Institutions

Louisiana State University Health Sciences Center Shreveport

University of Surrey

University of Alabama at Birmingham

University of North Carolina at Chapel Hill

Eunice Kennedy Shriver National Institute of Child Health and Human Development

Society for Maternal-Fetal Medicine

Louisiana State University

Baylor College of Medicine

Duke University

University of Cincinnati

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Inhibition of CYP1 by 7,8-dehydrorutaecarpine and its methoxylated derivatives

Journal of Food and Drug Analysis (2020)

Jen‐Chieh Tseng M.-J. Don David F. Lewis Simon Wang Yune‐Fang Ueng

IC50

10.38212/2224-6614.2400

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Citations (3)

Guide to Cytochromes P450: Structure and Function, Second Edition

David F. Lewis

Source

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Citations (21)

Molecular modelling and quantitative structure-activity relationship studies on the interaction of omeprazole with cytochrome P450 isozymes

Toxicology (1998)

David F. Lewis Brian G. Lake

Docking (animal)

10.1016/s0300-483x(97)00159-5

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Citations (38)

Increased Superoxide Generation and Decreased Stress Protein Hsp90 Expression in Human Umbilical Cord Vein Endothelial Cells (HUVECs) from Pregnancies Complicated by Preeclampsia

Hypertension in Pregnancy (2006)

Yang Gu David F. Lewis Yanping Zhang Lynn J. Groome Yuping Wang

Objective: Endothelial dysfunction is associated with increased oxidative stress in the vascular system in women with preeclampsia (PE), a hypertensive disorder occurring during human pregnancy. However, due to the nature of the disease, direct evidence of increased endothelial oxidative stress in the maternal vascular system at an in vivo situation is still lacking. We previously reported that primary cultured endothelial cells (ECs) from umbilical cords (HUVECs) from pregnancies complicated by PE exhibit phenotypic changes compared to those from normal pregnancies such as reduced eNOs expression associated with disorganized endothelial junction protein distribution and increased endothelial permeability. In this study, we sought to determine whether increased oxidative stress was also present in primary cultured HUVECs from women with PE. Methods: HUVECs were isolated from normal and PE pregnancies and EC oxidative stress was examined by superoxide generation using positive nuclear dihydroethidium (DHE) staining as an indicator. Since Hsp90 is believed to have protective effects on endothelial function, we also determined mRNA and protein expression for Hsp90. Using Hsp90 inhibitor geldanamycin (GA), we further determined the potential role of Hsp90 in superoxide generation, eNOs expression, and prostacyclin production of altered EC function associated with PE pregnancies. Results: We found that primary cultured ECs from PE pregnancies showed an increase in DHE positive cells, p < 0.01. Hsp90 protein expression was significantly decreased in ECs from PE compared with that from normal pregnancies, p < 0.05. Inhibition of Hsp90 by GA resulted in an increase in superoxide generation and a decrease in eNOs protein expression. Decreased prostacyclin production was also found in ECs treated with GA. Conclusion: These in vitro HUVEC data suggest that increased endothelial oxidative stress may also occur in the fetal compartment during preeclampsia.

Endothelial Dysfunction

10.1080/10641950600912950

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Citations (37)

Molecular Orbital Calculations on Tumour-Inhibitory Phenyl Aziridines: Qsars

Xenobiotica (1989)

David F. Lewis

1. Molecular orbital calculations, by the MINDO/3 method, on tumour-inhibitory phenyl aziridines are reported.2. The generation of quantitative structure-activity relationships (QSARs) shows that biological activity, chemical reactivity and hydrophobicity can be explained in terms of theoretically-derived electronic structural parameters.

MINDO

Reactivity

HOMO/LUMO

10.3109/00498258909042278

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Citations (10)

Measurement of the energy and power radiated by a pulsed blackbody x-ray source

Physical Review Special Topics - Accelerators and Beams (2006)

H.C. Ives W. A. Stygar D. L. Fehl Lynnette Ramirez S. C. Dropinski

We have developed a diagnostic system that measures the spectrally integrated (i.e. the total) energy and power radiated by a pulsed blackbody x-ray source. The total-energy-and-power (TEP) diagnostic system is optimized for blackbody temperatures between 50 and 350 eV. The system can view apertured sources that radiate energies and powers as high as 2 MJ and 200 TW, respectively, and has been successfully tested at 0.84 MJ and 73 TW on the $Z$ pulsed-power accelerator. The TEP system consists of two pinhole arrays, two silicon-diode detectors, and two thin-film nickel bolometers. Each of the two pinhole arrays is paired with a single silicon diode. Each array consists of a $38\ifmmode\times\else\texttimes\fi{}38$ square array of 10-$\ensuremath{\mu}\mathrm{m}$-diameter pinholes in a 50-$\ensuremath{\mu}\mathrm{m}$-thick tantalum plate. The arrays achromatically attenuate the x-ray flux by a factor of $\ensuremath{\sim}1800$. The use of such arrays for the attenuation of soft x rays was first proposed by Turner and co-workers [Rev. Sci. Instrum. 70, 656 (1999)]. The attenuated flux from each array illuminates its associated diode; the diode's output current is recorded by a data-acquisition system with 0.6-ns time resolution. The arrays and diodes are located 19 and 24 m from the source, respectively. Because the diodes are designed to have an approximately flat spectral sensitivity, the output current from each diode is proportional to the x-ray power. The nickel bolometers are fielded at a slightly different angle from the array-diode combinations, and view (without pinhole attenuation) the same x-ray source. The bolometers measure the total x-ray energy radiated by the source and---on every shot---provide an in situ calibration of the array-diode combinations. Two array-diode pairs and two bolometers are fielded to reduce random uncertainties. An analytic model (which accounts for pinhole-diffraction effects) of the sensitivity of an array-diode combination is presented.

Black-body radiation

Effective radiated power

Pinhole (optics)

10.1103/physrevstab.9.110401

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Citations (16)

Amino acid residue 250 has a functional role in the assembly of rabbit liver microsomal cytochrome P450 2B4

IUBMB Life (1998)

Johannes Schulze Michael Lehnerer David F. Lewis Peter Hlavica

Abstract Cytochrome P450 2B4 lacking amino acids 2‐27, CYP2B4 (Δ2‐27), was mutated at position 250 and expressed in E.coli fused toglutathione S‐transferase. Expression of the E250S variant (holo‐plus apoenzyme) proceeded to an extent comparable with that of CYP2B4 (Δ2‐27), while the protein level of the E250P mutant averaged 42% that of the control pigment. Comparison of these data with the corresponding reduced CO difference spectra of the various CYP2B4 (Δ2‐27) forms revealed that, in the control and E250S preparations, about 90% and 44%, respectively, of the total amount of hemoprotein present existed in the form of holoenzyme, whereas the E250P derivative failed to produce a reduced carbonyl complex. Thus, replacement of the negatively charged E250 with an uncharged, polar serine residue substantially hampered assembly of CYP2B4 (Δ2‐27); introduction of an α‐helix‐disrupting proline completely blocked the formation of holoenzyme. These phenomena suggested that the negative charge of E250, residing in the putative G helix, underwent pairing with some positively charged group, possibly H285 located in the I helix. Deletion of the negative charge obviously perturbed the active‐site geometry such as to affect both the incorporation and/or retention of the heme ligand and the spectral binding of substrates such as hexobarbital.

Residue (chemistry)

Hemeprotein

Helix (gastropod)

10.1080/15216549800202232

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Citations (3)

Downregulation of miR-126-3p expression contributes to increased inflammatory response in placental trophoblasts in preeclampsia

Journal of Reproductive Immunology (2021)

Xiaodan Chu Yang Gu Wenji Sheng Jingxia Sun John A. Morgan

Trophoblast

Syncytiotrophoblasts

Hypoxia

10.1016/j.jri.2021.103281

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Citations (14)

Structural determinations of four mono- and binuclear tertiary phosphine and arsine complexes of copper(I) chloride

Inorganic Chemistry (1976)

J. T. Gill J. J. Mayerle P. S. Welcker David F. Lewis DAVID A. UCKO

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural determinations of four mono- and binuclear tertiary phosphine and arsine complexes of copper(I) chlorideJ. T. Gill, J. J. Mayerle, P. S. Welcker, D. F. Lewis, D. A. Ucko, D. J. Barton, D. Stowens, and S. J. LippardCite this: Inorg. Chem. 1976, 15, 5, 1155–1168Publication Date (Print):May 1, 1976Publication History Published online1 May 2002Published inissue 1 May 1976https://doi.org/10.1021/ic50159a035RIGHTS & PERMISSIONSArticle Views428Altmetric-Citations121LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. These metrics are regularly updated to reflect usage leading up to the last few days.Citations are the number of other articles citing this article, calculated by Crossref and updated daily. Find more information about Crossref citation counts.The Altmetric Attention Score is a quantitative measure of the attention that a research article has received online. Clicking on the donut icon will load a page at altmetric.com with additional details about the score and the social media presence for the given article. Find more information on the Altmetric Attention Score and how the score is calculated. Share Add toView InAdd Full Text with ReferenceAdd Description ExportRISCitationCitation and abstractCitation and referencesMore Options Share onFacebookTwitterWechatLinked InReddit PDF (2 MB) Get e-AlertsSupporting Info (1)»Supporting Information Supporting Information Get e-Alerts

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10.1021/ic50159a035

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Citations (141)

Improving the screening accuracy for preterm labor: is the combination of fetal fibronectin and cervical length in symptomatic patients a useful predictor of preterm birth? A systematic review

American Journal of Obstetrics and Gynecology (2012)

Emily DeFranco David F. Lewis Anthony Odibo

Fetal fibronectin

10.1016/j.ajog.2012.12.015

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Citations (116)