Chengkai Lu

Chinese Academy of Sciences

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Jianqiang Wu

Anhui Agricultural University

Christian Hettenhausen

Riemser Pharma (Germany)

Jinfeng Qi

Kunming Institute of Botany

Yuxing Xu

Union Hospital

Jingxiong Zhang

Kunming Institute of Botany

Saif Ul Malook

University of Florida

Gang Liang

Xishuangbanna Tropical Botanical Garden

Jinpeng Wan

Xishuangbanna Tropical Botanical Garden

Jing Li

Beijing Center for Disease Prevention and Control

Lei Wang

Wuhan Polytechnic University

Cooperative Institutions

Chinese Academy of Sciences

University of Chinese Academy of Sciences

Xishuangbanna Tropical Botanical Garden

Kunming Institute of Botany

Chinese Academy of Agricultural Sciences

Beijing Botanical Garden

Sichuan University

Ministry of Agriculture and Rural Affairs

Chinese Academy of Medical Sciences & Peking Union Medical College

Lanzhou University

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IRON MAN interacts with BRUTUS to maintain iron homeostasis in Arabidopsis

Proceedings of the National Academy of Sciences (2021)

Yang Li Chengkai Lu Chen Yang Li Rihua Lei Meng Pu

IRON MAN (IMA) peptides, a family of small peptides, control iron (Fe) transport in plants, but their roles in Fe signaling remain unclear. BRUTUS (BTS) is a potential Fe sensor that negatively regulates Fe homeostasis by promoting the ubiquitin-mediated degradation of bHLH105 and bHLH115, two positive regulators of the Fe deficiency response. Here, we show that IMA peptides interact with BTS. The C-terminal parts of IMA peptides contain a conserved BTS interaction domain (BID) that is responsible for their interaction with the C terminus of BTS. Arabidopsis thaliana plants constitutively expressing IMA genes phenocopy the bts-2 mutant. Moreover, IMA peptides are ubiquitinated and degraded by BTS. bHLH105 and bHLH115 also share a BID, which accounts for their interaction with BTS. IMA peptides compete with bHLH105/bHLH115 for interaction with BTS, thereby inhibiting the degradation of these transcription factors by BTS. Genetic analyses suggest that bHLH105/bHLH115 and IMA3 have additive roles and function downstream of BTS. Moreover, the transcription of both BTS and IMA3 is activated directly by bHLH105 and bHLH115 under Fe-deficient conditions. Our findings provide a conceptual framework for understanding the regulation of Fe homeostasis: IMA peptides protect bHLH105/bHLH115 from degradation by sequestering BTS, thereby activating the Fe deficiency response.

Homeostasis

10.1073/pnas.2109063118

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Citations (62)

Ultraviolet-B enhances the resistance of multiple plant species to lepidopteran insect herbivory through the jasmonic acid pathway

Scientific Reports (2018)

Jinfeng Qi Mou Zhang Chengkai Lu Christian Hettenhausen Qing Tan

Abstract Land plants protect themselves from ultraviolet-B (UV-B) by accumulating UV-absorbing metabolites, which may also function as anti-insect toxins. Previous studies have shown that UV-B enhances the resistance of different plant species to pierce-sucking pests; however, whether and how UV-B influences plant defense against chewing caterpillars are not well understood. Here we show that UV-B treatment increased Spodoptera litura herbivory-induced jasmonic acid (JA) production in Arabidopsis and thereby Arabidopsis exhibited elevated resistance to S . litura . Using mutants impaired in the biosynthesis of JA and the defensive metabolites glucosinolates (GSs), we show that the UV-B-induced resistance to S . litura is dependent on the JA-regulated GSs and an unidentified anti-insect metabolite(s). Similarly, UV-B treatment also enhanced the levels of JA-isoleucine conjugate and defense-related secondary metabolites in tobacco, rice, and maize after these plants were treated with simulated herbivory of lepidopteran insects; consistently, these plants showed elevated resistance to insect larvae. Using transgenic plants impaired in JA biosynthesis or signaling, we further demonstrate that the UV-B-enhanced defense responses also require the JA pathway in tobacco and rice. Our findings reveal a likely conserved JA-dependent mechanism by which UV-B enhances plant defense against lepidopteran insects.

Jasmonic acid

Spodoptera litura

Helicoverpa zea

Secondary metabolite

10.1038/s41598-017-18600-7

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Citations (53)

Table S5. from The oriental armyworm (Mythimna separata) feeding induces systemic defence responses within and between maize leaves

Figshare (2018)

Saif Ul Malook Jinfeng Qi Christian Hettenhausen Yuxing Xu Cuiping Zhang

Transcription factor genes up-regulated in systemic leave

Mythimna separata

Table (database)

10.6084/m9.figshare.7430105

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Additional file 2: of Comparative analysis of alfalfa (Medicago sativa L.) leaf transcriptomes reveals genotype-specific salt tolerance mechanisms

Figshare (2018)

Yunting Lei Yuxing Xu Christian Hettenhausen Chengkai Lu Guojing Shen

This excel file contains all of the additional Tables (Table S1-S9) associated with the manuscript. Each Table is in a different tab. Table numbers and titles are listed below: Table S1. Summary of clean reads and mapping ratio. Table S2. Number of annotated unigenes in alfalfa. Table S3. Annotations of DEGs in two cultivars of alfalfa. Table S4. The DEGs involved in ROS homeostasis in two cultivars. Table S5. The DEGs involved in Ca2+ signaling in two cultivars. Table S6. The DEGs involved in phytohormone biosynthesis in two cultivars. Table S7. The DEGs involved in ion transport in two cultivars. Table S8. The DEGs involved in transcription factor in two cultivars. Table S9. The photosynthesis-related DEGs in two cultivars. (XLSX 6789Â kb)

Medicago sativa

Medicago

10.6084/m9.figshare.5896315

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Table S1 from The oriental armyworm (Mythimna separata) feeding induces systemic defence responses within and between maize leaves

Figshare (2018)

Saif Ul Malook Jinfeng Qi Christian Hettenhausen Yuxing Xu Cuiping Zhang

Genes detected in all samples

Mythimna separata

Table (database)

10.6084/m9.figshare.7430102

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Forward genetic screen for auxin-deficient mutants by cytokinin

Scientific Reports (2015)

Lei Wu Pan Luo Dong‐Wei Di Li Wang Ming Wang

Abstract Identification of mutants with impairments in auxin biosynthesis and dynamics by forward genetic screening is hindered by the complexity, redundancy and necessity of the pathways involved. Furthermore, although a few auxin-deficient mutants have been recently identified by screening for altered responses to shade, ethylene, N-1-naphthylphthalamic acid (NPA) or cytokinin (CK), there is still a lack of robust markers for systematically isolating such mutants. We hypothesized that a potentially suitable phenotypic marker is root curling induced by CK, as observed in the auxin biosynthesis mutant CK-induced root curling 1 / tryptophan aminotransferase of Arabidopsis 1 ( ckrc1/taa1 ). Phenotypic observations, genetic analyses and biochemical complementation tests of Arabidopsis seedlings displaying the trait in large-scale genetic screens showed that it can facilitate isolation of mutants with perturbations in auxin biosynthesis, transport and signaling. However, unlike transport/signaling mutants, the curled (or wavy) root phenotypes of auxin-deficient mutants were significantly induced by CKs and could be rescued by exogenous auxins. Mutants allelic to several known auxin biosynthesis mutants were re-isolated, but several new classes of auxin-deficient mutants were also isolated. The findings show that CK-induced root curling provides an effective marker for discovering genes involved in auxin biosynthesis or homeostasis.

Genetic screen

10.1038/srep11923

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Genome-Wide Characterization of the Duf247 Gene Family in Rice

Chengkai Lu Feifei Zhang Jun Yang Jinpeng Wan Peng Xu

The domain of unknown function 247 (DUF247) protein family is a large group of proteins that contains a conserved domain with amino acids ranging from 67 to 711, but their function is unknown. In our study, we identified 69 genes that encode DUF247 proteins in the rice genome. We analyzed the structures, phylogenetic relationships, expression profiles, and protein subcellular localizations of these genes. The results showed that all OsDUF247 proteins could be classified into four clades based on their amino acid sequences. Some DUF247 genes with close phylogenetic relationships were located on the same chromosomes, which indicates that gene duplication may have driven the expansion of the DUF247 gene family in the rice genome. The tissue profile analysis showed that most DUF247 genes expressed at constitutive levels in seedlings, roots, stems, and leaves, except for seven genes in panicles. These seven genes were induced by various abiotic stress, including cold, drought, heat, hormone treatment, and especially salt, as demonstrated by further experimental analysis. DUF247 proteins containing transmembrane domains were predicted to be located on the membrane, suggesting their significant roles in rice development and adaptation to the environment. These findings provide useful information for future studies on the function of the DUF247 gene family in rice.

Characterization

10.2139/ssrn.4560842

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bHLH11 inhibits bHLH IVc proteins by recruiting the TOPLESS/TOPLESS-RELATED corepressors in Arabidopsis

bioRxiv (Cold Spring Harbor Laboratory) (2020)

Yang Li Rihua Lei Mengna Pu Yuerong Cai Chengkai Lu

ABSTRACT Iron (Fe) homeostasis is essential for plant growth and development. Many transcription factors play pivotal roles in the maintenance of Fe homeostasis. bHLH11 was identified as a negative transcription factor regulating Fe homeostasis, however, the underlying molecular mechanism remains elusive. We generated two loss-of-function bhlh11 mutants which display the enhanced sensitivity to Fe excess, the increased Fe accumulation and the elevated expression of Fe deficiency responsive genes. bHLH11 protein, localized in both the cytoplasm and nucleus, decreases in response to Fe deficiency. Coexpression assays indicate that bHLH IVc transcription factors (TFs) (bHLH34, bHLH104, bHLH105, and bHLH115) facilitate the nuclear accumulation of bHLH11 protein. Further analysis indicates that bHLH11 represses the transactivity of bHLH IVc TFs towards bHLH Ib genes ( bHLH38, bHLH39, bHLH100 , and bHLH101 ). bHLH11 contains two EAR motifs which are responsible for the repression function by recruiting the TOPLESS/TOPLESS-RELATED (TPL/TPRs) corepressors. Correspondingly, the expression of Fe uptake genes increases in the tpr1 tpr4 tpl mutant. Moreover, genetic analysis reveals that bHLH11 has functions independent of FIT. This study provides insights into the complicate Fe homeostasis signaling network. One-sentence summary bHLH IVc proteins promote the bHLH11 protein accumulation in the nucleus where bHLH11 inhibits the transcriptional activation ability of bHLH IVc via its EAR motifs recruiting the TOPLESS/TOPLESS-RELATED corepressors.

Transcription

Homeostasis

10.1101/2020.04.09.035097

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Argentaffine Cells in the Digestive Tract of Hibernating and Non-hibernating in Chinese Fire-bellied Newt (Cynops orientalis)

International Journal of Morphology (2012)

Zhaohui Xie Duanqiang Guo Chengkai Lu LI Boying Shan Lin-na

In order to further studying the relationship between the gastrointestinal endocrine cells and the hibernation of Chinese fire-bellied newt, histological techniques were used in this study to explore the distributed density and morphology of argentaffine cells in the digestive tract during hibernation and non-hibernation.The argentaffine cells could be observed throughout the digestive tract of Chinese fire-bellied newt during hibernation and non-hibernation and mainly distributed in the acinar epithelial cells, epithelial cells and intrinsic membrane.They had appeared in the shape of goblet, pyramid, ellipse, round, cone-shaped, spindle-shaped.The cytoplasm contains secretory granules at the basal regions with spherical nuclei in the basal regions.Most of them had processes in different directions, suggesting that the argentaffine cells have combined the function of endocrine and exocrine.The distribution density curves of argentaffine cells were generally wavelike during the two periods.It was highest in the body of stomach in hibernation, while in nonhibernation it was highest in the pyloric.Totally the density of argentaffine cells was higher in hibernation period than that in nonhibernation period.The results showed that the activity of the digestive tract was expectedly weakened during the hibernation, but the function of endocrine was actually strengthened.

Hibernating myocardium

Hibernation

10.4067/s0717-95022012000400021

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Genome-wide characterization and analysis of rice DUF247 gene family

Research Square (Research Square) (2024)

Feifei Zhang Jun Yang Amir Sohail Chengkai Lu Peng Xu

Abstract Background The domain of unknown function 247 (DUF247) proteins are involved in plant development and stress response. Rice is an important worldwide cereal crop, although an increasing number of DUF proteins have been identified, the understanding of DUF proteins is still very limited in rice. Results In this study, we identified 69 genes that encode DUF247 proteins in the rice (Oryza sativa) genome by homology searches and domain prediction. All the OsDUF247 proteins were classified into four major groups (I, II, III and IV) by phylogenetic analysis. Remarkably OsDUF247 genes clustered on the chromosomes solely show close phylogenetic relationships, suggesting that gene duplications driven the expansion of the DUF247 gene family in the rice genome. The tissue profile analysis showed that most DUF247 genes expressed at constitutive levels in seedlings, roots, stems, and leaves, except for seven genes (LOC_Os01g21670, LOC_Os03g19700, LOC_Os05g04060, LOC_Os08g26820, LOC_Os08g26840, LOC_Os08g26850 and LOC_Os09g13410) in panicles. These seven genes were induced by various abiotic stress, including cold, drought, heat, hormone treatment, and especially salt, as demonstrated by further experimental analysis. DUF247 proteins contain transmembrane domains located on the membrane, suggesting their significant roles in rice development and adaptation to the environment. Conclusions These findings lay the foundation for functional characterizations of DUF247 genes to unravel their exact role in rice cultivars.

Characterization

10.21203/rs.3.rs-3866022/v1

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Citations (1)